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Isolation, characterization and chemical synthesis of a new insect defensin from Chironomus plumosus (Diptera).

Abstract：

:Injection of low doses of bacteria into the aquatic larvae of the dipteran insect Chironomus plumosus induces the appearance in their hemolymph of a potent antibacterial activity. We have isolated two 36-residue peptides from this hemolymph which are active against Gram-positive bacteria. The peptides are novel members of the insect defensin family and their sequences present marked differences with those of insect defensins isolated from other dipteran species. We have developed a method for efficient renaturation of this cysteine-rich molecule and obtained a highly pure synthetic Chironomus defensin.

journal_name

Insect Biochem Mol Biol

journal_title

Insect biochemistry and molecular biology

authors

Lauth X,Nesin A,Briand JP,Roussel JP,Hetru C

doi

10.1016/s0965-1748(98)00101-5

subject

Has Abstract

pub_date

1998-12-01 00:00:00

pages

1059-66

issue

12

eissn

0965-1748

issn

1879-0240

pii

S0965174898001015

journal_volume

28

pub_type

杂志文章

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