Abstract:
:In the silkworm Bombyx mori, three fibroin genes, fibroin-heavy-chain (fibH), fibroin-light-chain (fibL) and fibrohexamerin (fhx), are coexpressed only in the posterior silk gland (PSG) cells, while the sericin genes encoding silk glue proteins are expressed in the middle silk gland (MSG) cells. Silk gland factor-2 (SGF-2) is a PSG-specific activator complex of fibH, composed of a LIM-homeodomain protein, Awh, and its cofactors, Ldb and Lcaf. We investigated whether SGF-2 can activate other fibroin genes using transgenic silkworms. The genes for Ldb and Lcaf were expressed ubiquitously in various tissues, while the gene for Awh was expressed strictly specific in PSG of the wild type silkworms. Misexpression of Awh in transgenic silkworms induced ectopic expression of fibL and fhx as well as fibH in MSG. Coincidently with the induction of fibL and fhx by Awh, binding of SGF-2 to the promoter of fibL and fhx was detected in vitro, and SGF-2 binds directly to the fhx core promoter. Ectopic expression of the fibroin genes was observed at high levels in the middle part of MSG. Moreover, fibL and fhx were induced in the anterior silk gland (ASG) of the transgenic silkworms, but fibH was not. These results indicate that Awh is a key activator of all three fibroin genes, and the activity is probably regulated in conjunction with additional factors.
journal_name
Insect Biochem Mol Bioljournal_title
Insect biochemistry and molecular biologyauthors
Kimoto M,Tsubota T,Uchino K,Sezutsu H,Takiya Sdoi
10.1016/j.ibmb.2014.11.003subject
Has Abstractpub_date
2015-01-01 00:00:00pages
29-35eissn
0965-1748issn
1879-0240pii
S0965-1748(14)00177-5journal_volume
56pub_type
杂志文章abstract::Salivary glands separated from internal tissues of the lone star tick, Amblyomma americanum, are competent to synthesize prostaglandins (PGs). Using an in vitro PG biosynthesis assay four major PGs, namely, PGA2/PGB2, PGD2, PGE2, and PGF2 alpha were synthesized. Under standard assay conditions PGA2/PGB2 was the predom...
journal_title:Insect biochemistry and molecular biology
pub_type: 杂志文章
doi:10.1016/s0965-1748(96)00094-x
更新日期:1997-03-01 00:00:00
abstract::The heterodimeric and homodimeric garlic lectins ASAI and ASAII were produced as recombinant proteins in the yeast Pichia pastoris. The proteins were purified as functional dimeric lectins, but underwent post-translational proteolysis. Recombinant ASAII was a single homogenous polypeptide which had undergone C-termina...
journal_title:Insect biochemistry and molecular biology
pub_type: 杂志文章
doi:10.1016/j.ibmb.2008.07.002
更新日期:2008-10-01 00:00:00
abstract::Sulfakinin (SK) is a sulfated insect neuropeptide that is best known for its function as a satiety factor. It displays structural and functional similarities with the vertebrate peptides gastrin and cholecystokinin. Peptidomic studies in multiple insects, crustaceans and arachnids have revealed the widespread occurren...
journal_title:Insect biochemistry and molecular biology
pub_type: 杂志文章
doi:10.1016/j.ibmb.2015.03.008
更新日期:2015-06-01 00:00:00
abstract::The Sf9 insect cell line, derived from Spodoptera frugiperda, was used to study the regulation of adenylate cyclase (AC) activity by octopamine receptors. The cyclic AMP (cAMP) production was stimulated in a concentration-dependent manner by octopamine. Octopamine also elicited a rise in cytosolic Ca(2+). The Ca(2+) e...
journal_title:Insect biochemistry and molecular biology
pub_type: 杂志文章
doi:10.1016/s0965-1748(01)00089-3
更新日期:2002-03-01 00:00:00
abstract::In this paper we review the current status of research on fatty acid absorption and conversion to diacylglycerol in the midgut. We further discuss how diacylglycerol may leave the midgut and associate with lipophorin in hemolymph. We review the present understanding of the role of the lipid transfer particle and lipop...
journal_title:Insect biochemistry and molecular biology
pub_type: 杂志文章,评审
doi:10.1016/s0965-1748(00)00102-8
更新日期:2001-01-01 00:00:00
abstract::Malaria transmission by the mosquito vector requires sporozoite invasion into mosquito salivary glands. Parasites probably enter the glands by specific receptor-ligand interactions with molecules on the surface of the glands. We have undertaken the characterization of salivary gland surface molecules of Aedes aegypti ...
journal_title:Insect biochemistry and molecular biology
pub_type: 杂志文章
doi:10.1016/s0965-1748(99)00025-9
更新日期:1999-06-01 00:00:00
abstract::This analysis considers the structural evidence of a ligand-binding function of the nuclear receptor ultraspiracle (USP). The positions and nature of residues in the ligand-binding domain of USP from six higher insects is evaluated in comparison to the function of conserved residues vertebrate receptors that have been...
journal_title:Insect biochemistry and molecular biology
pub_type: 杂志文章,评审
doi:10.1016/s0965-1748(00)00038-2
更新日期:2000-08-01 00:00:00
abstract::Juvenile hormone esterase (JHE, EC 3.1.1.1) from whole Drosophila melanogaster prepupae has previously been purified by selective precipitations, isoelectric focussing and two column chromatography steps. JHE bands from dried silver-stained SDS-PAGE gels of that material were digested with trypsin. The masses of the t...
journal_title:Insect biochemistry and molecular biology
pub_type: 杂志文章
doi:10.1016/s0965-1748(01)00035-2
更新日期:2001-04-27 00:00:00
abstract::Insect pheromone binding proteins (PBPs) are believed to solubilize and transport hydrophobic sex pheromones across sensillum lymph to membrane-associated pheromone receptors. To address the molecular mechanisms of PBPs in insect pheromone perception, we undertook a systemic study on the PBPs of the black cutworm Agro...
journal_title:Insect biochemistry and molecular biology
pub_type: 杂志文章
doi:10.1016/j.ibmb.2012.12.009
更新日期:2013-03-01 00:00:00
abstract::UDP-glycosyltransferases (UGTs) catalyze the conjugation of a variety of small lipophilic molecules with uridine diphosphate (UDP) sugars, altering them into more water-soluble metabolites. Thereby, UGTs play an important role in the detoxification of xenobiotics and in the regulation of endobiotics. Recently, the gen...
journal_title:Insect biochemistry and molecular biology
pub_type: 杂志文章
doi:10.1016/j.ibmb.2014.04.003
更新日期:2014-07-01 00:00:00
abstract::Two forms of farnesyl diphosphate synthase (FPPS) from the spruce budworm, Choristoneura fumiferana, and one from the armyworm Pseudaletia unipuncta, have been cloned and their catalytic properties assessed. The type-2 FPPS of C. fumiferana (CfFPPS2) was efficient in the prenyl coupling of DMAPP or GPP with [(14)C]IPP...
journal_title:Insect biochemistry and molecular biology
pub_type: 杂志文章
doi:10.1016/j.ibmb.2007.05.012
更新日期:2007-08-01 00:00:00
abstract::Injection of low doses of bacteria into the aquatic larvae of the dipteran insect Chironomus plumosus induces the appearance in their hemolymph of a potent antibacterial activity. We have isolated two 36-residue peptides from this hemolymph which are active against Gram-positive bacteria. The peptides are novel member...
journal_title:Insect biochemistry and molecular biology
pub_type: 杂志文章
doi:10.1016/s0965-1748(98)00101-5
更新日期:1998-12-01 00:00:00
abstract::The cDNAs of an ultraviolet (UV) and long-wavelength (LW) (green) absorbing rhodopsin of the bush brown Bicyclus anynana were partially identified. The UV sequence, encoding 377 amino acids, is 76-79% identical to the UV sequences of the papilionids Papilio glaucus and Papilio xuthus and the moth Manduca sexta. A dend...
journal_title:Insect biochemistry and molecular biology
pub_type: 杂志文章
doi:10.1016/s0965-1748(02)00058-9
更新日期:2002-11-01 00:00:00
abstract::To gain insight into the molecular repertoire of the adult female salivary glands of the tiger mosquito Aedes albopictus, we performed transcriptome and proteome analysis. cDNA clones were sequenced and assembled in clusters of related sequences and the corresponding genes assigned to one of three categories: housekee...
journal_title:Insect biochemistry and molecular biology
pub_type: 杂志文章
doi:10.1016/j.ibmb.2006.10.007
更新日期:2007-02-01 00:00:00
abstract::A cDNA clone containing a 921 bp open-reading frame (307 amino acids; 34 kDa) homologous to the TATA-binding protein (TBP) was isolated and sequenced from a Spodoptera frugiperda cell line that is commonly used in the baculovirus expression system. Analysis of the S. frugiperda TBP (SfTBP) sequence showed that the ami...
journal_title:Insect biochemistry and molecular biology
pub_type: 杂志文章
doi:10.1016/0965-1748(94)90057-4
更新日期:1994-07-01 00:00:00
abstract::The first full-length mRNA for vitellogenin (Vg) from ticks was sequenced. This also represents the first complete sequence of Vg from the Chelicerata and of a heme binding Vg. The Vg cDNA from the American dog tick, Dermacentor variabilis was 5744nt in length (GenBank Accession number AY885250), which coded for a pro...
journal_title:Insect biochemistry and molecular biology
pub_type: 杂志文章
doi:10.1016/j.ibmb.2007.01.004
更新日期:2007-04-01 00:00:00
abstract::The present investigation is an effort to determine the possible roles of microRNAs (miRNAs) in the regulation of protease gene expression in Helicoverpa armigera upon exposure to plant protease inhibitors (PIs). Using Illumina platform, deep sequencing of 12 small RNA libraries was performed from H. armigera larvae f...
journal_title:Insect biochemistry and molecular biology
pub_type: 杂志文章
doi:10.1016/j.ibmb.2014.09.008
更新日期:2014-11-01 00:00:00
abstract::To understand the role that 20-hydroxy-ecdysone (20E) plays during ovarian development in Bombyx mori, we have undertaken the cloning of the silkworm ecdysone receptor (EcR) and a study of its expression during follicular cell differentiation. We have cloned a cDNA that contains a complete open reading frame for a 68....
journal_title:Insect biochemistry and molecular biology
pub_type: 杂志文章
doi:10.1016/0965-1748(95)00024-p
更新日期:1995-07-01 00:00:00
abstract::The molt-intermolt cycle is an essential feature in holometabolous and hemimetabolous insects' development. In the silkworm, silk glands are under dramatic morphological and functional changes with fibroin genes' transcription being repeatedly turned off and on during the molt-intermolt cycles. However, the molecular ...
journal_title:Insect biochemistry and molecular biology
pub_type: 杂志文章
doi:10.1016/j.ibmb.2016.07.002
更新日期:2016-09-01 00:00:00
abstract::Trypsin-like enzymes from the salivary gland complex (SGC) of Lygus hesperus Knight were partially purified by preparative isoelectric focusing (IEF). Enzyme active against Nalpha-benzoyl-L-arginine-p-nitroanilide (BApNA) focused at approximately pH 10 during IEF. This alkaline fraction gave a single activity band whe...
journal_title:Insect biochemistry and molecular biology
pub_type: 杂志文章
doi:10.1016/s0965-1748(01)00123-0
更新日期:2002-04-01 00:00:00
abstract::A pheromonotropic peptide was isolated from brain-suboesophageal ganglion complexes of the adult female gypsy moth, Lymantria dispar, using a 5-step HPLC purification protocol and an in vivo bioassay in Helicoverpa zea. The intact peptide was sequenced by automated Edman degradation. The L. dispar pheromone biosynthes...
journal_title:Insect biochemistry and molecular biology
pub_type: 杂志文章
doi:10.1016/0965-1748(94)90111-2
更新日期:1994-09-01 00:00:00
abstract::Aminopeptidases N (APNs) are a class of ectoenzymes present in lepidopteran larvae midguts, involved in the Bacillus thuringiensis (Bt) toxins mode of action. In the present work, seven aminopeptidases have been cloned from the midgut of Ostrinia nubilalis, the major Lepidopteran corn pest in the temperate climates. S...
journal_title:Insect biochemistry and molecular biology
pub_type: 杂志文章
doi:10.1016/j.ibmb.2010.04.010
更新日期:2010-07-01 00:00:00
abstract::Rhodnius prolixus is an obligatorily hematophagous insect known as an important vector of Chagas disease. Autophagy is a conserved cellular mechanism that acts in response to nutrient starvation, where components of the cytoplasm are sequestered by a double membrane organelle, named autophagosome, which is targeted to...
journal_title:Insect biochemistry and molecular biology
pub_type: 杂志文章
doi:10.1016/j.ibmb.2020.103484
更新日期:2020-12-01 00:00:00
abstract::The sericin 1 primary transcript of the silkworm Bombyx mori is differentially spliced via a tissue- and developmentally-regulated process. From a middle silk gland cDNA library, we have elucidated the sequence of one of the four mRNAs, the 4.0 kb Ser1B mRNA. Determination of alternative or constitutive exons and intr...
journal_title:Insect biochemistry and molecular biology
pub_type: 杂志文章
doi:10.1016/s0965-1748(97)00022-2
更新日期:1997-05-01 00:00:00
abstract::BMCP18(2) is one of the major cuticle proteins identified in the larval cuticle of the silkworm, Bombyx mori. A genomic clone coding for BMCP18 was isolated from a B. mori genomic library, and its structure was analyzed. The BMCP18 gene consists of three exons interspersed by two introns. Bm1 element-like sequences we...
journal_title:Insect biochemistry and molecular biology
pub_type: 杂志文章
doi:10.1016/s0965-1748(00)00166-1
更新日期:2001-04-27 00:00:00
abstract::Phenoloxidase (PO), a melanin-synthesizing enzyme known to play an important role in insect defense, is found as a zymogen (ProPO) in hemolymph and cuticle, where it is activated by proteolysis. We characterized the first proPO cDNA in an eusocial insect, the Apis mellifera honey bee. The AmproPO cDNA contains an ORF ...
journal_title:Insect biochemistry and molecular biology
pub_type: 杂志文章
doi:10.1016/j.ibmb.2005.01.013
更新日期:2005-06-01 00:00:00
abstract::The acyl-CoA-binding proteins (ACBP) constitute a family of conserved proteins that bind acyl-CoA with high affinity and protect it from hydrolysis. Thus, ACBPs may have essential roles in basal cellular lipid metabolism. The genome of the insect Rhodnius prolixus encodes five ACBP genes similar to those described for...
journal_title:Insect biochemistry and molecular biology
pub_type: 杂志文章
doi:10.1016/j.ibmb.2016.03.002
更新日期:2016-05-01 00:00:00
abstract::Eicosanoids play crucial roles in mediating insect immune responses. In vertebrates, phospholipase A2 (PLA2) releases arachidonic acid (AA) from phospholipids (PLs) for biosynthesis of various eicosanoids. However, little AA is found in PLs of lepidopteran insects. Spodoptera exigua, a lepidopteran insect, is known to...
journal_title:Insect biochemistry and molecular biology
pub_type: 杂志文章
doi:10.1016/j.ibmb.2019.103179
更新日期:2019-08-01 00:00:00
abstract::There are multiple stages in the life cycle of Plasmodium that invade host cells. Molecular machinery involved is such host-pathogen interactions constitute excellent drug targets and/or vaccine candidates. A screen using a phage display library has previously demonstrated presence of enolase on the surface of the Pla...
journal_title:Insect biochemistry and molecular biology
pub_type: 杂志文章
doi:10.1016/j.ibmb.2015.11.004
更新日期:2016-01-01 00:00:00
abstract::Many insect developmental color changes are known to be regulated by both ecdysone and juvenile hormone. Yet the molecular mechanisms underlying this regulation have not been well understood. This review highlights the hormonal mechanisms involved in the regulation of two key enzymes [dopa decarboxylase (DDC) and phen...
journal_title:Insect biochemistry and molecular biology
pub_type: 杂志文章,评审
doi:10.1016/j.ibmb.2009.01.008
更新日期:2009-04-01 00:00:00