Bacillus thuringiensis Cry1Ac and Cry1Fa delta-endotoxin binding to a novel 110 kDa aminopeptidase in Heliothis virescens is not N-acetylgalactosamine mediated.

Abstract:

:We determined that Bacillus thuringiensis Cry1Ac and Cry1Fa delta-endotoxins recognize the same 110, 120 and 170 kDa aminopeptidase N (APN) molecules in brush border membrane vesicles (BBMV) from Heliothis virescens. The 110 kDa protein, not previously identified as an APN, contained a variant APN consensus sequence identical to that found in Helicoverpa punctigera APN 2. PCR amplification of H. virescens cDNA based on this sequence and a conserved APN motif yielded a 0.9 kb product that has 89% sequence homology with H. punctigera APN 2. Western blots revealed that the 110 kDa molecule was not recognized by soybean agglutinin, indicating the absence of GalNAc. A 125I labeled-Cry1Ac domain III mutant (509QNR(511)-AAA) that has an altered GalNAc binding pocket (Lee et al., Appl. Environ. Microbiol. 65 (1999) 4513) showed abolished binding to the 120 APN, reduced binding to the 170 kDa APN, and enhanced binding to the 110 kDa APN. Periodate treated H. virescens BBMV blots were also probed with 125I labeled-Cry1Ac and 509QNR(511)-AAA toxins. Both toxins still recognized the 110 kDa APN and a >210 kDa molecule which may be a cadherin-like protein. Additionally, 125I-(509)QNR(511)-AAA recognized periodate treated 170 kDa APN. Results indicate that the 110 kDa APN is distinct from other Cry1 toxin binding APNs and may be the first described Cry1Ac-binding APN that does not contain GalNAc.

authors

Banks DJ,Jurat-Fuentes JL,Dean DH,Adang MJ

doi

10.1016/s0965-1748(01)00038-8

subject

Has Abstract

pub_date

2001-07-26 00:00:00

pages

909-18

issue

9

eissn

0965-1748

issn

1879-0240

pii

S0965-1748(01)00038-8

journal_volume

31

pub_type

杂志文章
  • Identification of a Bombyx collagenous protein with multiple short domains of Gly-Xaa-Yaa repeats: cDNA characterization and regulation of expression.

    abstract::Using a differential screening strategy, a 2 kb cDNA was isolated from a differentiating Bombyx mori imaginal wing discs library. The available reading frame of 1710 bp specifies a conceptual protein with two distinct regions: a 395 amino acid region corresponding to 105 repetitions of the Gly-Xaa-Yaa triple-helical f...

    journal_title:Insect biochemistry and molecular biology

    pub_type: 杂志文章

    doi:10.1016/s0965-1748(96)00032-x

    authors: Chareyre P,Besson MT,Fourche J,Bosquet G

    更新日期:1996-07-01 00:00:00

  • Host hemolymph proteins and protein digestion in larval Habrobracon hebetor (Hymenoptera: braconidae).

    abstract::Host plasma proteins and protein digestion in larval parasitoids were studied during trophic interactions of the ectoparasitoid Habrobracon hebetor Say (Hymenoptera: Braconidae), with a host, larvae of the Indianmeal moth, Plodia interpunctella Hübner (Lepidoptera: Pyralidae). We could detect no apparent differences i...

    journal_title:Insect biochemistry and molecular biology

    pub_type: 杂志文章

    doi:10.1016/s0965-1748(00)00066-7

    authors: Baker JE,Fabrick JA

    更新日期:2000-10-01 00:00:00

  • Localization of the Drosophila male accessory gland protein Acp36DE in the mated female suggests a role in sperm storage.

    abstract::In many insect species, sperm transferred in a single mating are stored by the female in specialized organs and are gradually used to fertilize eggs. Thus, insects must have mechanisms to ensure that substantial numbers of sperm reach and become stored in the storage organs. We report here that a glycoprotein, Acp36DE...

    journal_title:Insect biochemistry and molecular biology

    pub_type: 杂志文章

    doi:10.1016/s0965-1748(96)00064-1

    authors: Bertram MJ,Neubaum DM,Wolfner MF

    更新日期:1996-09-01 00:00:00

  • A RhoGAP protein as a main immune suppressive factor in the Leptopilina boulardi (Hymenoptera, Figitidae)-Drosophila melanogaster interaction.

    abstract::To protect its eggs, the endoparasitoid wasp Leptopilina boulardi injects immune suppressive factors into Drosophila melanogaster host larvae. These factors are localized in the female long gland and reservoir. We analyzed the protein content of these tissues and found that it strongly differed between virulent and av...

    journal_title:Insect biochemistry and molecular biology

    pub_type: 杂志文章

    doi:10.1016/j.ibmb.2004.10.004

    authors: Labrosse C,Stasiak K,Lesobre J,Grangeia A,Huguet E,Drezen JM,Poirie M

    更新日期:2005-02-01 00:00:00

  • Functional expression and characterisation of a gut facilitative glucose transporter, NlHT1, from the phloem-feeding insect Nilaparvata lugens (rice brown planthopper).

    abstract::Phloem-sap feeding Hemipteran insects have access to a sucrose-rich diet but are dependent on sucrose hydrolysis and hexose transport for carbon nutrition. A cDNA library from Nilaparvata lugens (rice brown planthopper) was screened for clones encoding potential transmembrane transporters. A selected cDNA, NlHT1, enco...

    journal_title:Insect biochemistry and molecular biology

    pub_type: 杂志文章

    doi:10.1016/j.ibmb.2007.07.001

    authors: Price DR,Wilkinson HS,Gatehouse JA

    更新日期:2007-11-01 00:00:00

  • Transcriptome comparison between honey bee queen- and worker-destined larvae.

    abstract::Caste differentiation in the female honey bee is one of the most intriguing polyphenism phenomena. This developmental switch depends on the differential expression of entire suites of the genes involved in the larval fate between the queens and workers. In this study, we compared the transcriptome differences between ...

    journal_title:Insect biochemistry and molecular biology

    pub_type: 杂志文章

    doi:10.1016/j.ibmb.2012.05.004

    authors: Chen X,Hu Y,Zheng H,Cao L,Niu D,Yu D,Sun Y,Hu S,Hu F

    更新日期:2012-09-01 00:00:00

  • Structural characteristics of a novel antifreeze protein from the longhorn beetle Rhagium inquisitor.

    abstract::Antifreeze proteins (AFPs) are characterized by their capacity to inhibit the growth of ice and are produced by a variety of polar fish, terrestrial arthropods and other organisms inhabiting cold environments. This capacity reflects their role as stabilizers of supercooled body fluids. The longhorn beetle Rhagium inqu...

    journal_title:Insect biochemistry and molecular biology

    pub_type: 杂志文章

    doi:10.1016/j.ibmb.2010.11.002

    authors: Kristiansen E,Ramløv H,Højrup P,Pedersen SA,Hagen L,Zachariassen KE

    更新日期:2011-02-01 00:00:00

  • Substrate-stereoselectivity of a high-affinity glutamate transporter cloned from the CNS of the cockroach Diploptera punctata.

    abstract::A cDNA encoding a Na(+)-dependent glutamate transporter has been cloned from the brain of the cockroach Diploptera punctata. The cDNA encodes a transporter protein of 481 amino acids, designated DipEAAT1, which when expressed in baculovirus infected insect cells, resulted in a 40-50 fold increase in [(3)H]L-glutamate ...

    journal_title:Insect biochemistry and molecular biology

    pub_type: 杂志文章

    doi:10.1016/s0965-1748(00)00004-7

    authors: Donly C,Jevnikar J,McLean H,Caveney S

    更新日期:2000-05-01 00:00:00

  • Biotin-containing proteins of the insect nervous system, a potential source of interference with immunocytochemical localization procedures.

    abstract::When the biotinylated Manduca sexta adipokinetic hormone gene was used as a probe for in situ hybridization, the intrinsic neurosecretory cells were stained with a biotin detection system that contained streptavidin or avidin. Further experiments showed that the DNA probe was not necessary for staining these cells by ...

    journal_title:Insect biochemistry and molecular biology

    pub_type: 杂志文章

    doi:10.1016/0965-1748(94)00095-y

    authors: Ziegler R,Engler DL,Davis NT

    更新日期:1995-05-01 00:00:00

  • Rhodnius prolixus supergene families of enzymes potentially associated with insecticide resistance.

    abstract::Chagas disease or American trypanosomiasis, is a potentially life-threatening illness caused by the protozoan parasite, Trypanosoma cruzi. Once known as an endemic health problem of poor rural populations in Latin American countries, it has now spread worldwide. The parasite is transmitted by triatomine bugs, of which...

    journal_title:Insect biochemistry and molecular biology

    pub_type: 杂志文章

    doi:10.1016/j.ibmb.2015.06.005

    authors: Schama R,Pedrini N,Juárez MP,Nelson DR,Torres AQ,Valle D,Mesquita RD

    更新日期:2016-02-01 00:00:00

  • Aberrant splicing of the Drosophila melanogaster phenylalanine hydroxylase pre-mRNA caused by the insertion of a B104/roo transposable element in the Henna locus.

    abstract::We report the insertion of the transposable element B104 in the Phenylalanine hydroxylase gene of the Drosophila mutant Henna-recessive 3. Its presence alters the Phenylalanine hydroxylase splicing pattern, producing at least two aberrant mRNAs which contain part of the B104 sequence interrupting the coding region. Th...

    journal_title:Insect biochemistry and molecular biology

    pub_type: 杂志文章

    doi:10.1016/s0965-1748(99)00002-8

    authors: Ruiz-Vázquez P,Silva FJ

    更新日期:1999-04-01 00:00:00

  • Inhibition of insect juvenile hormone epoxide hydrolase: asymmetric synthesis and assay of glycidol-ester and epoxy-ester inhibitors of trichoplusia ni epoxide hydrolase.

    abstract::Juvenile hormone (JH) undergoes metabolic degradation by two major pathways involving JH esterase and JH epoxide hydrolase (EH). While considerable effort has been focussed on the study of JH esterase and the development of inhibitors for this enzyme, much less has been reported on the study of JH-EH. In this work, th...

    journal_title:Insect biochemistry and molecular biology

    pub_type: 杂志文章

    doi:10.1016/s0965-1748(00)00048-5

    authors: Linderman RJ,Roe RM,Harris SV,Thompson DM

    更新日期:2000-08-01 00:00:00

  • Purification, properties and heteromeric association of type-1 and type-2 lepidopteran farnesyl diphosphate synthases.

    abstract::Two forms of farnesyl diphosphate synthase (FPPS) from the spruce budworm, Choristoneura fumiferana, and one from the armyworm Pseudaletia unipuncta, have been cloned and their catalytic properties assessed. The type-2 FPPS of C. fumiferana (CfFPPS2) was efficient in the prenyl coupling of DMAPP or GPP with [(14)C]IPP...

    journal_title:Insect biochemistry and molecular biology

    pub_type: 杂志文章

    doi:10.1016/j.ibmb.2007.05.012

    authors: Sen SE,Cusson M,Trobaugh C,Béliveau C,Richard T,Graham W,Mimms A,Roberts G

    更新日期:2007-08-01 00:00:00

  • The role of a single gene encoding the Single von Willebrand factor C-domain protein (SVC) in bumblebee immunity extends beyond antiviral defense.

    abstract::The Single von Willebrand factor C-domain proteins (SVCs) are a group of short proteins mainly found in arthropods. They are proposed to be responsive in relation to environmental challenges including the nutritional status, bacterial and viral infections. The SVC protein Vago acts as a cross-talk molecule between the...

    journal_title:Insect biochemistry and molecular biology

    pub_type: 杂志文章

    doi:10.1016/j.ibmb.2017.10.002

    authors: Wang H,Smagghe G,Meeus I

    更新日期:2017-12-01 00:00:00

  • Biosynthesis of L-alanine, a major amino acid of fibroin in Samia cynthia ricini.

    abstract::The derivation of alanine in fibroin was investigated using NMR and selective isotopic labelling. 2H2O infused orally into 5th instar larvae was incorporated into the proton of the methyl group of alanine in fibroin. Proton exchange among alanine, glycine and serine was also found. Incorporation of 13C from [2-(13)C]a...

    journal_title:Insect biochemistry and molecular biology

    pub_type: 杂志文章

    doi:10.1016/s0965-1748(99)00120-4

    authors: Osanai M,Okudaira M,Naito J,Demura M,Asakura T

    更新日期:2000-03-01 00:00:00

  • Two single mutations commonly cause qualitative change of nonspecific carboxylesterases in insects.

    abstract::Carboxylesterases provide key mechanisms of resistance to insecticides, particularly organophosphates (OPs), in insects. One resistance mechanism is a qualitative change in the properties of a carboxylesterase. Two mutant forms, G151D and W271L, have been observed, mostly in dipteran species, to affect substrate speci...

    journal_title:Insect biochemistry and molecular biology

    pub_type: 杂志文章

    doi:10.1016/j.ibmb.2010.09.004

    authors: Cui F,Lin Z,Wang H,Liu S,Chang H,Reeck G,Qiao C,Raymond M,Kang L

    更新日期:2011-01-01 00:00:00

  • Distribution of cuticular protein mRNAs in silk moth integument and imaginal discs.

    abstract::The distributions of mRNAs for two cuticular proteins of Hyalophora cecropia were examined with RT-PCR and in situ hybridization. For major regions of larval and pupal cuticle, there was a strong correspondence between the type of cuticle and the predominant cuticular protein message found. Epidermal cells underlying ...

    journal_title:Insect biochemistry and molecular biology

    pub_type: 杂志文章

    doi:10.1016/j.ibmb.2003.06.010

    authors: Gu S,Willis JH

    更新日期:2003-12-01 00:00:00

  • Biochemical characterization of maintenance DNA methyltransferase DNMT-1 from silkworm, Bombyx mori.

    abstract::DNA methylation is an important epigenetic mechanism involved in gene expression of vertebrates and invertebrates. In general, DNA methylation profile is established by de novo DNA methyltransferases (DNMT-3A, -3B) and maintainance DNA methyltransferase (DNMT-1). DNMT-1 has a strong substrate preference for hemimethyl...

    journal_title:Insect biochemistry and molecular biology

    pub_type: 杂志文章

    doi:10.1016/j.ibmb.2015.01.008

    authors: Mitsudome T,Mon H,Xu J,Li Z,Lee JM,Patil AA,Masuda A,Iiyama K,Morokuma D,Kusakabe T

    更新日期:2015-03-01 00:00:00

  • A non-invasive method for silencing gene transcription in honeybees maintained under natural conditions.

    abstract::In the Apis mellifera post-genomic era, RNAi protocols have been used in functional approaches. However, sample manipulation and invasive methods such as injection of double-stranded RNA (dsRNA) can compromise physiology and survival. To circumvent these problems, we developed a non-invasive method for honeybee gene k...

    journal_title:Insect biochemistry and molecular biology

    pub_type: 杂志文章

    doi:10.1016/j.ibmb.2008.10.011

    authors: Nunes FM,Simões ZL

    更新日期:2009-02-01 00:00:00

  • Structural analysis of gene encoding cuticle protein BMCP18, and characterization of its putative transcription factor in the silkworm, Bombyx mori.

    abstract::BMCP18(2) is one of the major cuticle proteins identified in the larval cuticle of the silkworm, Bombyx mori. A genomic clone coding for BMCP18 was isolated from a B. mori genomic library, and its structure was analyzed. The BMCP18 gene consists of three exons interspersed by two introns. Bm1 element-like sequences we...

    journal_title:Insect biochemistry and molecular biology

    pub_type: 杂志文章

    doi:10.1016/s0965-1748(00)00166-1

    authors: Togawa T,Shofuda K,Yaginuma T,Tomino S,Nakato H,Izumi S

    更新日期:2001-04-27 00:00:00

  • Molecular characterization of the VLDL receptor homolog mediating binding of lipophorin in oocyte of the mosquito Aedes aegypti.

    abstract::Lipophorin (Lp) functions as a yolk protein precursor in the mosquito Aedes aegypti and it is internalized via receptor-mediated endocytosis (Insect Biochem. Mol. Biol., 30 (2000) 1161). We cloned and molecularly characterized a putative mosquito ovarian lipophorin receptor (AaLpRov) cDNA. The cDNA has a length of 346...

    journal_title:Insect biochemistry and molecular biology

    pub_type: 杂志文章

    doi:10.1016/s0965-1748(01)00068-6

    authors: Cheon HM,Seo SJ,Sun J,Sappington TW,Raikhel AS

    更新日期:2001-06-22 00:00:00

  • Rhodnius heme-binding protein (RHBP) is a heme source for embryonic development in the blood-sucking bug Rhodnius prolixus (Hemiptera, Reduviidae).

    abstract::We have previously shown that Rhodnius prolixus' eggs and hemolymph are pink due to the presence of the hemeprotein Rhodnius heme-binding protein (RHBP). In the hemolymph it functions as an antioxidant. Nevertheless, its function in eggs has not been determined. Here we present evidence that RHBP is a source of heme f...

    journal_title:Insect biochemistry and molecular biology

    pub_type: 杂志文章

    doi:10.1016/s0965-1748(01)00163-1

    authors: Braz GR,Moreira MF,Masuda H,Oliveira PL

    更新日期:2002-04-01 00:00:00

  • Odorant-binding protein-based identification of natural spatial repellents for the African malaria mosquito Anopheles gambiae.

    abstract::There is increasing interest in the development of effective mosquito repellents of natural origin to reduce transmission of diseases such as malaria and yellow fever. To achieve this we have employed an in vitro competition assay involving odorant-binding proteins (OBPs) of the malaria mosquito, Anopheles gambiae, wi...

    journal_title:Insect biochemistry and molecular biology

    pub_type: 杂志文章

    doi:10.1016/j.ibmb.2018.03.008

    authors: Kröber T,Koussis K,Bourquin M,Tsitoura P,Konstantopoulou M,Awolola TS,Dani FR,Qiao H,Pelosi P,Iatrou K,Guerin PM

    更新日期:2018-05-01 00:00:00

  • Inhibition of cAMP signalling cascade-mediated Ca2+ influx by a prothoracicostatic peptide (Mas-MIP I) via dihydropyridine-sensitive Ca2+ channels in the prothoracic glands of the silkworm, Bombyx mori.

    abstract::Measurements of Ca(2+) influx in Fura-2/AM loaded prothoracic glands (PGs) of the silkworm, Bombyx mori, after application of forskolin or the cAMP analogue, 8-bromo-cAMP, showed a steady increase in [Ca(2+)](i), which was of extracellular origin and was inhibited, in both cases, by the dihydropyridine (DHP) derivativ...

    journal_title:Insect biochemistry and molecular biology

    pub_type: 杂志文章

    doi:10.1016/s0965-1748(02)00206-0

    authors: Dedos SG,Birkenbeil H

    更新日期:2003-02-01 00:00:00

  • Pharmacological profiles of recombinant and native insect nicotinic acetylcholine receptors.

    abstract::Nicotinic acetylcholine receptors (nAChRs) are targets for insect-selective neonicotinoid insecticides exemplified by imidacloprid (IMI) and mammalian-selective nicotinoids including nicotine and epibatidine (EPI). Despite their importance, insect nAChRs are poorly understood compared with their vertebrate counterpart...

    journal_title:Insect biochemistry and molecular biology

    pub_type: 杂志文章

    doi:10.1016/j.ibmb.2005.08.006

    authors: Tomizawa M,Millar NS,Casida JE

    更新日期:2005-12-01 00:00:00

  • Resistance to diamide insecticides in diamondback moth, Plutella xylostella (Lepidoptera: Plutellidae) is associated with a mutation in the membrane-spanning domain of the ryanodine receptor.

    abstract::Diamide insecticides such as chlorantraniliprole and flubendiamide are a new class of insecticide that selectively target insect ryanodine receptors (RyR), a distinct class of homo-tetrameric calcium release channels which play a pivotal role in calcium homeostasis in numerous cell types. Resistance to these insectici...

    journal_title:Insect biochemistry and molecular biology

    pub_type: 杂志文章

    doi:10.1016/j.ibmb.2012.09.001

    authors: Troczka B,Zimmer CT,Elias J,Schorn C,Bass C,Davies TG,Field LM,Williamson MS,Slater R,Nauen R

    更新日期:2012-11-01 00:00:00

  • Mutation of a novel ABC transporter gene is responsible for the failure to incorporate uric acid in the epidermis of ok mutants of the silkworm, Bombyx mori.

    abstract::ok mutants of the silkworm, Bombyx mori, exhibit highly translucent larval skin resulting from the inability to incorporate uric acid into the epidermal cells. Here we report the identification of a gene responsible for the ok mutation using positional cloning and RNAi experiments. In two independent ok mutant strains...

    journal_title:Insect biochemistry and molecular biology

    pub_type: 杂志文章

    doi:10.1016/j.ibmb.2013.03.011

    authors: Wang L,Kiuchi T,Fujii T,Daimon T,Li M,Banno Y,Kikuta S,Kikawada T,Katsuma S,Shimada T

    更新日期:2013-07-01 00:00:00

  • Non-active site residues Cys69 and Asp150 affected the enzymatic properties of glutathione S-transferase AdGSTD3-3.

    abstract::To elucidate how non-active site residues support the catalytic function, five selected residues of AdGSTD3-3 isoenzyme were changed to AdGSTD1-1 residues by means of site-directed mutagenesis. Analysis of the kinetic parameters indicated that Cys69Gln and Asp150Ser showed marked differences in Vmax and Km compared wi...

    journal_title:Insect biochemistry and molecular biology

    pub_type: 杂志文章

    doi:10.1016/s0965-1748(03)00103-6

    authors: Wongtrakul J,Udomsinprasert R,Ketterman AJ

    更新日期:2003-10-01 00:00:00

  • Cloning and functional characterization of the Anopheles albimanus DMT1/NRAMP homolog: implications in iron metabolism in mosquitoes.

    abstract::In addition to its wide role in metabolism, iron in insects has been implicated in vitellogenesis and the immune response. The NRAMP family comprises a well-conserved family of divalent cation transporters in metazoans. To gain insight on the role of NRAMP in Anopheles albimanus, we cloned a cDNA encoding a 571-residu...

    journal_title:Insect biochemistry and molecular biology

    pub_type: 杂志文章

    doi:10.1016/j.ibmb.2007.02.009

    authors: Martínez-Barnetche J,García Solache M,Neri Lecona A,Tello López AT,del Carmen Rodríguez M,Gamba G,Vázquez N,Rodríguez MH,Lanz-Mendoza H

    更新日期:2007-06-01 00:00:00

  • Characterization of midgut trypsin-like enzymes and three trypsinogen cDNAs from the lesser grain borer, Rhyzopertha dominica (Coleoptera: Bostrichidae).

    abstract::Protein digestion in the lesser grain borer, Rhyzopertha dominica (F.) (Coleoptera: Bostrichidae), results from the action of a complex of serine proteinases present in the midgut. In this study we partially characterized trypsin-like enzyme activity against N-alpha-benzoyl-L-arginine p-nitroanilide (BApNA) in midgut ...

    journal_title:Insect biochemistry and molecular biology

    pub_type: 杂志文章

    doi:10.1016/s0965-1748(99)00081-8

    authors: Zhu YC,Baker JE

    更新日期:1999-12-01 00:00:00