当前位置： SCI文献检索 > INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY期刊下所有文献 > Contribution of Ldace1 gene to acetylcholinesterase activity in Colorado potato beetle.

Contribution of Ldace1 gene to acetylcholinesterase activity in Colorado potato beetle.

Abstract：

:The Colorado potato beetle (CPB), Leptinotarsa decemlineata is an important economic pest of potato worldwide. Resistance to organophosphates and carbamates in CPB has been associated in some cases to point mutations in the acetylcholinesterase (AChE) gene Ldace2, an orthologue of Drosophila melanogaster Dmace2. In this paper we report cloning and sequencing of Ldace1, an orthologue of Anopheles gambiae Agace1 that was previously unknown in CPB. The Ldace1 coding enzyme contains all residues conserved in a functionally active AChE. Ldace1 is expressed at higher levels (between 2- and 11-fold) than Ldace2 in embryos, in the four larval instars and in adults. Specific interference of Ldace1 by means of dsRNA injection resulted in a reduction of AChE activity to an approximate 50% compared to control, whilst interference of Ldace2 reduced AChE activity to an approximate 85%. Analysis of zymograms of AChE activity after interference indicates that LdAChE1 is the enzyme predominantly responsible for the activity visualised. Interference of Ldace1 in CPB adults caused a significant increase in mortality (43%) as early as three days post-injection (p.i.), suggesting the essential role of Ldace1. Interference of Ldace2 also caused a significant increase in mortality (29%) compared to control, although at seven days p.i. The effect of the interference of Ldace1 on susceptibility to the organophosphate chlorpyrifos points out that LdAChE1 could be a main target for this insecticide. In the light of our results, studies associating resistance in CPB to mutations in Ldace2 should be reviewed, taking into consideration analysis of the Ldace1 gene.

journal_name

Insect Biochem Mol Biol

journal_title

Insect biochemistry and molecular biology

authors

Revuelta L,Ortego F,Díaz-Ruíz JR,Castañera P,Tenllado F,Hernández-Crespo P

doi

10.1016/j.ibmb.2011.06.001

subject

Has Abstract

pub_date

2011-10-01 00:00:00

pages

795-803

issue

10

eissn

0965-1748

issn

1879-0240

pii

S0965-1748(11)00123-8

journal_volume

41

pub_type

杂志文章

相关文献

INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY文献大全
  • Host hemolymph proteins and protein digestion in larval Habrobracon hebetor (Hymenoptera: braconidae).

    abstract：:Host plasma proteins and protein digestion in larval parasitoids were studied during trophic interactions of the ectoparasitoid Habrobracon hebetor Say (Hymenoptera: Braconidae), with a host, larvae of the Indianmeal moth, Plodia interpunctella Hübner (Lepidoptera: Pyralidae). We could detect no apparent differences i...

    journal_title：Insect biochemistry and molecular biology

    pub_type： 杂志文章

    doi：10.1016/s0965-1748(00)00066-7

    authors： Baker JE,Fabrick JA

    更新日期：2000-10-01 00:00:00

  • Silencing the odorant receptor co-receptor RproOrco affects the physiology and behavior of the Chagas disease vector Rhodnius prolixus.

    abstract：:Olfaction is one of the main sensory modalities that allow insects to interpret their environment. Several proteins, including odorant-binding proteins (OBPs) and odorant receptors (ORs), are involved in this process. Odorant receptors are ion channels formed by a binding unit OR and an odorant receptor co-receptor (O...

    journal_title：Insect biochemistry and molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.ibmb.2015.02.012

    authors： Franco TA,Oliveira DS,Moreira MF,Leal WS,Melo AC

    更新日期：2016-02-01 00:00:00

  • A protein from the cabbage looper, Trichoplusia ni, regulated by a bacterial infection is homologous to 3-dehydroecdysone 3beta-reductase.

    abstract：:During the screening of immune-regulated genes from the cabbage looper, Trichoplusia ni, a 3-dehydroecdysone 3beta-reductase homologue (DERH) was cloned. In the course of development, 3-dehydroecdysone 3beta-reductase mediates the conversion of 3-dehydroecdysone (3dE) secreted from the prothoracic glands to ecdysone (...

    journal_title：Insect biochemistry and molecular biology

    pub_type： 杂志文章

    doi：10.1016/s0965-1748(01)00145-x

    authors： Lundström A,Kang D,Liu G,Fernandez C,Warren JT,Gilbert LI,Steiner H

    更新日期：2002-08-01 00:00:00

  • Sindbis virus induces transport processes and alters expression of innate immunity pathway genes in the midgut of the disease vector, Aedes aegypti.

    abstract：:The midgut of hematophagous insects is the initial site of infection by arthropod-borne viruses (arboviruses) and plays a crucial role in vector competence. To further understand processes that occur in the midgut in response to infection by an arbovirus, DNA microarrays were used to analyze gene expression changes fo...

    journal_title：Insect biochemistry and molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.ibmb.2005.07.006

    authors： Sanders HR,Foy BD,Evans AM,Ross LS,Beaty BJ,Olson KE,Gill SS

    更新日期：2005-11-01 00:00:00

  • Acp70A regulates Drosophila pheromones through juvenile hormone induction.

    abstract：:Mated Drosophila melanogaster females show a decrease in mating receptivity, enhanced ovogenesis, egg-laying and activation of juvenile hormone (JH) production. Components in the male seminal fluid, especially the sex peptide ACP70A stimulate these responses in females. Here we demonstrate that ACP70A is involved in t...

    journal_title：Insect biochemistry and molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.ibmb.2014.11.008

    authors： Bontonou G,Shaik HA,Denis B,Wicker-Thomas C

    更新日期：2015-01-01 00:00:00

  • Drosophila odorant receptors are novel seven transmembrane domain proteins that can signal independently of heterotrimeric G proteins.

    abstract：:Olfaction in Drosophila is mediated by a large family of membrane-bound odorant receptor proteins (Ors). In heterologous cells, we investigated whether the structural features and signalling mechanisms of ligand-binding Drosophila Ors are consistent with them being G protein-coupled receptors (GPCRs). The detailed mem...

    journal_title：Insect biochemistry and molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.ibmb.2008.05.002

    authors： Smart R,Kiely A,Beale M,Vargas E,Carraher C,Kralicek AV,Christie DL,Chen C,Newcomb RD,Warr CG

    更新日期：2008-08-01 00:00:00

  • The 30kP protease A responsible for 30-kDa yolk protein degradation of the silkworm, Bombyx mori: cDNA structure, developmental change and regulation by feeding.

    abstract：:We have cloned and sequenced the cDNA encoding the major component (43-kDa peptide) of 30kP protease A which selectively hydrolyzes 30-kDa yolk proteins of the silkworm, Bombix mori. The deduced amino acid sequence consisted of 318 amino acids and shared sequences conserved in many serine proteases. Northern blot anal...

    journal_title：Insect biochemistry and molecular biology

    pub_type： 杂志文章

    doi：10.1016/s0965-1748(00)00135-1

    authors： Maki N,Yamashita O

    更新日期：2001-03-15 00:00:00

  • Expression of genes encoding proteins involved in ecdysteroidogenesis in the female mosquito, Aedes aegypti.

    abstract：:A blood meal induces the ovaries of female Aedes aegypti mosquitoes to produce ecdysteroid hormones that regulate many processes required for egg maturation. Various proteins involved in the intracellular transport and biosynthesis of ecdysteroid precursors have been identified by analysis of Drosophila melanogaster m...

    journal_title：Insect biochemistry and molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.ibmb.2005.01.011

    authors： Sieglaff DH,Duncan KA,Brown MR

    更新日期：2005-05-01 00:00:00

  • Intersex regulates female external genital and imaginal disc development in the silkworm.

    abstract：:As a component of the mediator complex, the intersex (ix) gene product is involved in the sex determination pathway of the Drosophila melanogaster. IX functions together with the female-specific product of doublesex (dsx) at the bottom of the hierarchy to implement female sexual differentiation. Here we analyzed the f...

    journal_title：Insect biochemistry and molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.ibmb.2019.02.003

    authors： Xu J,Yu Y,Chen K,Huang Y

    更新日期：2019-05-01 00:00:00

  • Biosynthesis of L-alanine, a major amino acid of fibroin in Samia cynthia ricini.

    abstract：:The derivation of alanine in fibroin was investigated using NMR and selective isotopic labelling. 2H2O infused orally into 5th instar larvae was incorporated into the proton of the methyl group of alanine in fibroin. Proton exchange among alanine, glycine and serine was also found. Incorporation of 13C from [2-(13)C]a...

    journal_title：Insect biochemistry and molecular biology

    pub_type： 杂志文章

    doi：10.1016/s0965-1748(99)00120-4

    authors： Osanai M,Okudaira M,Naito J,Demura M,Asakura T

    更新日期：2000-03-01 00:00:00

  • In silico approach of azadirachtin binding with actins.

    abstract：:In silico docking analysis reported here suggests that insect cellular cytoskeletal beta-actin could be the target of Azadirachtin A (Aza-the principle bioactive compound of neem seeds). The best docking energy of -40.09 kcal/mol at 8.73 A RMSD and predicted hydrogen bond between Arg210 and carboxymethyl group of Aza ...

    journal_title：Insect biochemistry and molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.ibmb.2007.03.010

    authors： Pravin Kumar R,Manoj MN,Kush A,Annadurai RS

    更新日期：2007-06-01 00:00:00

  • Identification of G protein coupled receptors for opsines and neurohormones in Rhodnius prolixus. Genomic and transcriptomic analysis.

    abstract：:The importance of Chagas disease motivated the scientific effort to obtain the complete genomic sequence of the vector species Rhodnius prolixus, this information is also relevant to the understanding of triatomine biology in general. The central nervous system is the key regulator of insect physiology and behavior. N...

    journal_title：Insect biochemistry and molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.ibmb.2015.05.003

    authors： Ons S,Lavore A,Sterkel M,Wulff JP,Sierra I,Martínez-Barnetche J,Rodriguez MH,Rivera-Pomar R

    更新日期：2016-02-01 00:00:00

  • Glutathione S-transferase in the defence against pyrethroids in insects.

    abstract：:The correlation between the natural levels of GST and the tolerance to the insecticide decamethrin (dMT), as well as the interaction between the molecules of affinity purified enzyme and the insecticide were investigated in order to collect further information on the obscure role of the Glutathione S-transferase syste...

    journal_title：Insect biochemistry and molecular biology

    pub_type： 杂志文章

    doi：10.1016/s0965-1748(00)00123-5

    authors： Kostaropoulos I,Papadopoulos AI,Metaxakis A,Boukouvala E,Papadopoulou-Mourkidou E

    更新日期：2001-03-15 00:00:00

  • Identification of a Bombyx collagenous protein with multiple short domains of Gly-Xaa-Yaa repeats: cDNA characterization and regulation of expression.

    abstract：:Using a differential screening strategy, a 2 kb cDNA was isolated from a differentiating Bombyx mori imaginal wing discs library. The available reading frame of 1710 bp specifies a conceptual protein with two distinct regions: a 395 amino acid region corresponding to 105 repetitions of the Gly-Xaa-Yaa triple-helical f...

    journal_title：Insect biochemistry and molecular biology

    pub_type： 杂志文章

    doi：10.1016/s0965-1748(96)00032-x

    authors： Chareyre P,Besson MT,Fourche J,Bosquet G

    更新日期：1996-07-01 00:00:00

  • The ecdysone regulatory cascade and ovarian development in lepidopteran insects: insights from the silkmoth paradigm.

    abstract：:The developing ovariole of the silkmoth Bombyx mori represents an excellent model for studies on the changes that occur in gene expression during the execution of long-term developmental programs. All stages of follicle development, which differ from each other by 2-2.5 h of developmental time, are organized in a sing...

    journal_title：Insect biochemistry and molecular biology

    pub_type： 杂志文章,评审

    doi：10.1016/j.ibmb.2003.06.012

    authors： Swevers L,Iatrou K

    更新日期：2003-12-01 00:00:00

  • Transcriptional activation of the Drosophila ecdysone receptor by insect and plant ecdysteroids.

    abstract：:A number of insect ecdysteroids, plant ecdysteroids and juvenoids were assayed for their ability to activate Drosophila nuclear receptors in transfected tissue culture cells. Discrete modifications to 20-hydroxyecdysone, the apparent natural ligand for the ecdysone receptor (EcR), conferred dramatic changes on the tra...

    journal_title：Insect biochemistry and molecular biology

    pub_type： 杂志文章

    doi：10.1016/s0965-1748(00)00075-8

    authors： Baker KD,Warren JT,Thummel CS,Gilbert LI,Mangelsdorf DJ

    更新日期：2000-11-01 00:00:00

  • Molecular characterization of an arachnid sodium channel gene from the varroa mite (Varroa destructor).

    abstract：:Voltage-gated sodium channels are essential for the generation and propagation of action potentials in most excitable cells. They are the target sites of several classes of insecticides and acaricides. Isolation of full-length sodium channel cDNA is a critical and often difficult step toward an understanding of insect...

    journal_title：Insect biochemistry and molecular biology

    pub_type： 杂志文章

    doi：10.1016/s0965-1748(03)00068-7

    authors： Wang R,Huang ZY,Dong K

    更新日期：2003-07-01 00:00:00

  • The dual roles of Armigeres subalbatus prophenoloxidase V in parasite melanization and egg chorion melanization in the mosquito Ar. subalbatus.

    abstract：:Phenoloxidases (POs) play key roles in various physiological functions in insects, e.g., cuticular sclerotization, wound healing, egg tanning, cuticle formation and melanotic encapsulaction of pathogens. Previously, we identified five POs, designated As-pro-PO I-V, from the mosquito Armigeres subalbatus and demonstrat...

    journal_title：Insect biochemistry and molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.ibmb.2015.07.016

    authors： Tsao IY,Chen JW,Li CJ,Lo HL,Christensen BM,Chen CC

    更新日期：2015-09-01 00:00:00

  • Considerations on the structural evidence of a ligand-binding function of ultraspiracle, an insect homolog of vertebrate RXR.

    abstract：:This analysis considers the structural evidence of a ligand-binding function of the nuclear receptor ultraspiracle (USP). The positions and nature of residues in the ligand-binding domain of USP from six higher insects is evaluated in comparison to the function of conserved residues vertebrate receptors that have been...

    journal_title：Insect biochemistry and molecular biology

    pub_type： 杂志文章,评审

    doi：10.1016/s0965-1748(00)00038-2

    authors： Jones G,Jones D

    更新日期：2000-08-01 00:00:00

  • The control of BhB10-1 gene expression in the salivary gland of Bradysia hygida (Diptera, Sciaridae) is disrupted in vivo by a delayed effect of cycloheximide.

    abstract：:BhB10-1 is an amplified gene present in DNA puff B10. This gene is very active in the salivary gland regions S1 and S3 at the end of the larval development. Two transcripts of this gene, 1.3 and 1.1 kb in size, were detected. A secretory protein, SP23, is the product of BhB10-1. In this work, we present evidence suppo...

    journal_title：Insect biochemistry and molecular biology

    pub_type： 杂志文章

    doi：10.1016/s0965-1748(01)00156-4

    authors： Conacci ME,Coelho PS,Valente V,de F Sousa J,Paçó-Larson ML,de Almeida JC

    更新日期：2002-07-01 00:00:00

  • Molecular cloning of a multidomain cysteine protease and protease inhibitor precursor gene from the tobacco hornworm (Manduca sexta) and functional expression of the cathepsin F-like cysteine protease domain.

    abstract：:A Manduca sexta (tobacco hornworm) cysteine protease inhibitor, MsCPI, purified from larval hemolymph has an apparent molecular mass of 11.5 kDa, whereas the size of the mRNA is very large (∼9 kilobases). MsCPI cDNA consists of a 9,273 nucleotides that encode a polypeptide of 2,676 amino acids, which includes nine tan...

    journal_title：Insect biochemistry and molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.ibmb.2010.08.003

    authors： Miyaji T,Murayama S,Kouzuma Y,Kimura N,Kanost MR,Kramer KJ,Yonekura M

    更新日期：2010-12-01 00:00:00

  • Horizontal gene transfer and functional diversification of plant cell wall degrading polygalacturonases: Key events in the evolution of herbivory in beetles.

    abstract：:Plant cell walls are the largest reservoir of organic carbon on earth. To breach and utilize this carbohydrate-rich protective barrier, microbes secrete plant cell wall degrading enzymes (PCWDEs) targeting pectin, cellulose and hemicelluloses. There is a growing body of evidence that genomes of some herbivorous insect...

    journal_title：Insect biochemistry and molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.ibmb.2014.06.008

    authors： Kirsch R,Gramzow L,Theißen G,Siegfried BD,Ffrench-Constant RH,Heckel DG,Pauchet Y

    更新日期：2014-09-01 00:00:00

  • The biosynthesis and processing of vitellogenin in the fat bodies of females and males of the cockroach Leucophaea maderae.

    abstract：:The juvenile hormone analog (JHA) methoprene was used to induce the synthesis of the yolk protein precursor vitellogenin (Vg) in adult females and males of the cockroach Leucophaea maderae. The female- and male-produced vitellogenin (VgF and VgM, respectively) contained polypeptides of 112, 95, 92, and 54 kDa. Also pr...

    journal_title：Insect biochemistry and molecular biology

    pub_type： 杂志文章

    doi：10.1016/s0965-1748(97)00071-4

    authors： Don-Wheeler G,Engelmann F

    更新日期：1997-11-01 00:00:00

  • Functional expression and characterisation of a gut facilitative glucose transporter, NlHT1, from the phloem-feeding insect Nilaparvata lugens (rice brown planthopper).

    abstract：:Phloem-sap feeding Hemipteran insects have access to a sucrose-rich diet but are dependent on sucrose hydrolysis and hexose transport for carbon nutrition. A cDNA library from Nilaparvata lugens (rice brown planthopper) was screened for clones encoding potential transmembrane transporters. A selected cDNA, NlHT1, enco...

    journal_title：Insect biochemistry and molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.ibmb.2007.07.001

    authors： Price DR,Wilkinson HS,Gatehouse JA

    更新日期：2007-11-01 00:00:00

  • Characterization of cholesterol transport from midgut to fat body in Manduca sexta larvae.

    abstract：:Using in vitro methods, we investigated the transfer of cholesterol from larval Manduca sexta midgut to the hemolymph lipoprotein, lipophorin, and the transfer of cholesterol from lipophorin to larval fat body. In the midgut, transfer of free cholesterol shows saturation kinetics, but the apparent Km is higher than th...

    journal_title：Insect biochemistry and molecular biology

    pub_type： 杂志文章

    doi：10.1016/s0965-1748(02)00051-6

    authors： Yun HK,Jouni ZE,Wells MA

    更新日期：2002-09-01 00:00:00

  • Vasopressin-like peptide and its receptor function in an indirect diuretic signaling pathway in the red flour beetle.

    abstract：:The insect arginine vasopressin-like (AVPL) peptide is of special interest because of its potential function in the regulation of diuresis. Genome sequences of the red flour beetle Tribolium castaneum yielded the genes encoding AVPL and AVPL receptor, whereas the homologous sequences are absent in the genomes of the f...

    journal_title：Insect biochemistry and molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.ibmb.2008.04.006

    authors： Aikins MJ,Schooley DA,Begum K,Detheux M,Beeman RW,Park Y

    更新日期：2008-07-01 00:00:00

  • Partial characterization of oligosaccharides expressed on midgut microvillar glycoproteins of the mosquito, Anopheles stephensi Liston.

    abstract：:Midguts of the malaria-transmitting mosquito, Anopheles stephensi, were homogenized and microvillar membranes prepared by calcium precipitation and differential centrifugation. Oligosaccharides present on the microvillar glycoproteins were identified by lectin blotting before and after in vitro and in situ treatments ...

    journal_title：Insect biochemistry and molecular biology

    pub_type： 杂志文章

    doi：10.1016/s0965-1748(01)00040-6

    authors： Wilkins S,Billingsley PF

    更新日期：2001-09-01 00:00:00

  • Functional evaluation of Heat Shock Proteins 70 (HSP70/HSC70) on Rhodnius prolixus (Hemiptera, Reduviidae) physiological responses associated with feeding and starvation.

    abstract：:Blood-sucking vectors must overcome thermal stress caused by intake of proportionally large amounts of warm blood from their hosts. In response to this, Heat Shock Proteins (HSPs) such as the widely studied HSP70 family (the inducible HSP70 and the cognate form HSC70, known for their role in preserving essential cellu...

    journal_title：Insect biochemistry and molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.ibmb.2016.07.011

    authors： Paim RMM,Araujo RN,Leis M,Sant'anna MRV,Gontijo NF,Lazzari CR,Pereira MH

    更新日期：2016-10-01 00:00:00

  • Medfly transposable elements: diversity, evolution, genomic impact and possible applications.

    abstract：:The medfly genome has been shown to contain a rich assortment of transposable elements from the mariner, Tc1, hAT and gypsy/Ty3 families. These elements display different levels of diversity, abundance and distribution in the genome. The presence of actively transposing elements in the medfly genome is revealed by hyb...

    journal_title：Insect biochemistry and molecular biology

    pub_type： 杂志文章,评审

    doi：10.1016/j.ibmb.2003.06.015

    authors： Gomulski LM,Torti C,Murelli V,Bonizzoni M,Gasperi G,Malacrida AR

    更新日期：2004-02-01 00:00:00

  • Identification of cytochrome P450 and glutathione-S-transferase genes preferentially expressed in chemosensory organs of the swallowtail butterfly, Papilio xuthus L.

    abstract：:The swallowtail butterfly, Papilio xuthus L., feeds exclusively on members of the plant family, Rutaceae. Female butterflies lay eggs in response to specific chemicals contained in their host plants. They perceive a variety of polar compounds as oviposition stimulants through the tarsal chemosensilla of the foreleg by...

    journal_title：Insect biochemistry and molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.ibmb.2005.03.013

    authors： Ono H,Ozaki K,Yoshikawa H

    更新日期：2005-08-01 00:00:00