Abstract：
:cAMP-dependent-protein kinase (PKA) is a central player of the adipokinetic signal that controls the mobilization of stored lipids in the fat body. Previous studies showed that adipokinetic hormone (AKH) rapidly activates PKA from the fat body of Manduca sexta (Arrese et al. (J. Lipid. Res. 40(3): 556)). As a part of our investigation on lipolysis in insects, here we report the purification and characterization of the catalytic subunit of PKA from the fat body of M. sexta and its role in the direct activation of the TG lipase in vitro. PKA was purified to apparent homogeneity and the identity of the protein was confirmed by MALDI-TOF and Western blot analysis. The enzyme showed a high affinity for Mg-ATP (Km = 39 microM) and Kemptide (Km = 31 microM) and was strongly inhibited by the PKA specific inhibitors PKI 5-24 and H89. Manduca sexta PKA only recognized serine residues as phosphate acceptor; theronine or tyrosine containing peptides were not phosphorylated. Purified fat body TG-lipase proved to be a good substrate of the purified kinase. However, phosphorylation of the lipase did not enhance the lipolytic activity of the enzyme in vitro. These results suggest that, besides lipase phosphorylation, the mechanism of AKH-induced activation of the lipolysis requires the involvement of other proteins and/or signals.
journal_name
Insect Biochem Mol Bioljournal_title
Insect biochemistry and molecular biologyauthors
Patel R,Soulages JL,Wells MA,Arrese ELdoi
10.1016/j.ibmb.2004.08.008subject
Has Abstractpub_date
2004-12-01 00:00:00pages
1269-79issue
12eissn
0965-1748issn
1879-0240pii
S0965-1748(04)00152-3journal_volume
34pub_type
杂志文章abstract：:We have previously cloned and characterized BmJHE, a juvenile hormone (JH)-selective esterase (JHE) that is important for JH titer regulation in the silkworm Bombyx mori. Here, we sought to determine whether multiple genes might function as JH-specific esterase in this species. We searched for putative carboxyl/cholin...
journal_title：Insect biochemistry and molecular biology
pub_type： 杂志文章
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更新日期：2019-01-01 00:00:00
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更新日期：2008-08-01 00:00:00
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journal_title：Insect biochemistry and molecular biology
pub_type： 杂志文章
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更新日期：2006-03-01 00:00:00
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journal_title：Insect biochemistry and molecular biology
pub_type： 杂志文章
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pub_type： 杂志文章
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pub_type： 杂志文章
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更新日期：2002-06-01 00:00:00
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pub_type： 杂志文章
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pub_type： 杂志文章
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journal_title：Insect biochemistry and molecular biology
pub_type： 杂志文章
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更新日期：1995-07-01 00:00:00
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journal_title：Insect biochemistry and molecular biology
pub_type： 杂志文章
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更新日期：2001-04-27 00:00:00
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journal_title：Insect biochemistry and molecular biology
pub_type： 杂志文章
doi：10.1016/j.ibmb.2018.10.003
更新日期：2018-12-01 00:00:00
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journal_title：Insect biochemistry and molecular biology
pub_type： 杂志文章
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更新日期：2012-07-01 00:00:00
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journal_title：Insect biochemistry and molecular biology
pub_type： 杂志文章
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更新日期：2019-05-01 00:00:00
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pub_type： 杂志文章
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更新日期：2008-10-01 00:00:00
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pub_type： 杂志文章
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更新日期：2016-10-01 00:00:00
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journal_title：Insect biochemistry and molecular biology
pub_type： 杂志文章
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更新日期：2007-05-01 00:00:00
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journal_title：Insect biochemistry and molecular biology
pub_type： 杂志文章
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更新日期：2017-09-01 00:00:00
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journal_title：Insect biochemistry and molecular biology
pub_type： 杂志文章,评审
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更新日期：2006-04-01 00:00:00
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journal_title：Insect biochemistry and molecular biology
pub_type： 杂志文章
doi：10.1016/j.ibmb.2017.05.005
更新日期：2017-07-01 00:00:00
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pub_type： 杂志文章
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更新日期：2011-03-01 00:00:00
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journal_title：Insect biochemistry and molecular biology
pub_type： 杂志文章
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更新日期：2012-09-01 00:00:00
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pub_type： 杂志文章
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更新日期：2019-08-01 00:00:00
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journal_title：Insect biochemistry and molecular biology
pub_type： 杂志文章
doi：10.1016/j.ibmb.2004.01.003
更新日期：2004-04-01 00:00:00
abstract：:Outbreaks of the black army cutworm, Actebia fennica, are associated with recently burned sites, where larvae feed on early successional plants. In the present paper we show that smoke volatiles stimulate juvenile hormone biosynthesis in virgin females, resulting in a more rapid rate of oocyte maturation and a signifi...
journal_title：Insect biochemistry and molecular biology
pub_type： 杂志文章
doi：10.1016/s0965-1748(00)00058-8
更新日期：2000-08-01 00:00:00
