当前位置： SCI文献检索 > INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY期刊下所有文献 > Subunit composition of pro-phenol oxidase from Manduca sexta: molecular cloning of subunit ProPO-P1.

Subunit composition of pro-phenol oxidase from Manduca sexta: molecular cloning of subunit ProPO-P1.

Abstract：

:Phenol oxidase (PO) is known to play an important role in defense mechanisms in insect immunity. It is present as a zymogen in insect hemolymph, and can be activated by a specific proteolytic reaction that is stimulated by microbial cell wall components. The pro-phenol oxidase (pro-PO) purified from the larval hemolymph of Manduca sexta contains two polypeptides in equal amounts as revealed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). A cDNA for one of the polypeptides, now designated proPO-p2, has been isolated (Hall et al. (1995) Proc. Natl. Acad. Sci. USA, 92, 7764-7768). We purified pro-PO from plasma of M. sexta and characterized its subunit composition. A cDNA for M. sexta proPO-p1 was isolated from a larval hemocyte cDNA library. M. sexta proPO-p1 is 78% identical in amino acid sequence to Bombyx mori proPO-p1, but only 50% to M. sexta or B. mori proPO-p2. Immunofluorescence labelling and in situ hybridization showed that the pro-PO is synthesized in a single hemocyte type, the oenocytoids. Analysis of pro-PO by size exclusion high-pressure liquid chromatography (HPLC) revealed that pro-PO exists as monomeric, dimeric, trimeric or multimeric structures depending on the ionic strength. All of these isoforms of the protein have phenol oxidase activity upon activation with a detergent, cetylpyridinium chloride. In analysis by non-denaturing PAGE, the majority of the purified pro-PO was present as two dimers of distinct mobility (fast and slow forms). Both forms contain proPO-p1 and proPO-p2, suggesting that they are heterodimers. Individual larvae can contain the slow form, the fast form, or both, which suggests that the slow and fast forms of proPO are allelic variants. These results indicate that there are two pro-PO genes in M. sexta, which are coordinately expressed in oenocytoids, and whose products form predominantly heterodimers in plasma.

journal_name

Insect Biochem Mol Biol

journal_title

Insect biochemistry and molecular biology

authors

Jiang H,Wang Y,Ma C,Kanost MR

doi

10.1016/s0965-1748(97)00066-0

subject

Has Abstract

pub_date

1997-10-01 00:00:00

pages

835-50

issue

10

eissn

0965-1748

issn

1879-0240

pii

S0965-1748(97)00066-0

journal_volume

27

pub_type

杂志文章

相关文献

INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY文献大全
  • Efficient disruption of endogenous Bombyx gene by TAL effector nucleases.

    abstract：:Engineered nucleases are proteins that are able to cleave DNA at specified sites in the genome. These proteins have recently been used for gene targeting in a number of organisms. We showed earlier that zinc finger nucleases (ZFNs) can be used for generating gene-specific mutations in Bombyx mori by an error-prone DNA...

    journal_title：Insect biochemistry and molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.ibmb.2012.10.011

    authors： Sajwan S,Takasu Y,Tamura T,Uchino K,Sezutsu H,Zurovec M

    更新日期：2013-01-01 00:00:00

  • The E75 gene of Manduca sexta and comparison with its Drosophila homolog.

    abstract：:The ecdysone-inducible E75 gene responsible for the 75B puff of Drosophila melanogaster encodes a family of proteins which are members of the steroid receptor superfamily. These proteins are believed to be involved in the regulation of ecdysone response. In order to investigate the evolutionary conservation of E75, we...

    journal_title：Insect biochemistry and molecular biology

    pub_type： 杂志文章

    doi：10.1016/0965-1748(93)90086-8

    authors： Segraves WA,Woldin C

    更新日期：1993-01-01 00:00:00

  • The wing-specific cuticular protein LmACP7 is essential for normal wing morphogenesis in the migratory locust.

    abstract：:Wings are an indispensable structure in many insects for their foraging, courtship, escape from predators, and migration. Cuticular proteins are major components of the insect cuticle and wings, but there is limited information on how cuticular proteins may play an essential role in wing morphogenesis. We identified a...

    journal_title：Insect biochemistry and molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.ibmb.2019.103206

    authors： Zhao X,Gou X,Liu W,Ma E,Moussian B,Li S,Zhu K,Zhang J

    更新日期：2019-09-01 00:00:00

  • Comparative genomics of insect juvenile hormone biosynthesis.

    abstract：:The biosynthesis of insect juvenile hormone (JH) and its neuroendocrine control are attractive targets for chemical control of insect pests and vectors of disease. To facilitate the molecular study of JH biosynthesis, we analyzed ESTs from the glands producing JH, the corpora allata (CA) in the cockroach Diploptera pu...

    journal_title：Insect biochemistry and molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.ibmb.2006.01.013

    authors： Noriega FG,Ribeiro JM,Koener JF,Valenzuela JG,Hernandez-Martinez S,Pham VM,Feyereisen R

    更新日期：2006-04-01 00:00:00

  • Non-active site residues Cys69 and Asp150 affected the enzymatic properties of glutathione S-transferase AdGSTD3-3.

    abstract：:To elucidate how non-active site residues support the catalytic function, five selected residues of AdGSTD3-3 isoenzyme were changed to AdGSTD1-1 residues by means of site-directed mutagenesis. Analysis of the kinetic parameters indicated that Cys69Gln and Asp150Ser showed marked differences in Vmax and Km compared wi...

    journal_title：Insect biochemistry and molecular biology

    pub_type： 杂志文章

    doi：10.1016/s0965-1748(03)00103-6

    authors： Wongtrakul J,Udomsinprasert R,Ketterman AJ

    更新日期：2003-10-01 00:00:00

  • Circadian transcription factors differentially regulate features of the adult overwintering diapause in the Northern house mosquito, Culex pipiens.

    abstract：:The short days of late summer and early fall are the environmental cues that most temperate insects and other animals use to predict winter's arrival. Although it is still unclear precisely how insects measure daylength, there is mounting evidence that the circadian clock regulates seasonal responses including photope...

    journal_title：Insect biochemistry and molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.ibmb.2020.103365

    authors： Chang V,Meuti ME

    更新日期：2020-06-01 00:00:00

  • Cloning and functional characterization of the Anopheles albimanus DMT1/NRAMP homolog: implications in iron metabolism in mosquitoes.

    abstract：:In addition to its wide role in metabolism, iron in insects has been implicated in vitellogenesis and the immune response. The NRAMP family comprises a well-conserved family of divalent cation transporters in metazoans. To gain insight on the role of NRAMP in Anopheles albimanus, we cloned a cDNA encoding a 571-residu...

    journal_title：Insect biochemistry and molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.ibmb.2007.02.009

    authors： Martínez-Barnetche J,García Solache M,Neri Lecona A,Tello López AT,del Carmen Rodríguez M,Gamba G,Vázquez N,Rodríguez MH,Lanz-Mendoza H

    更新日期：2007-06-01 00:00:00

  • Molecular and functional characterisation of resilin across three insect orders.

    abstract：:Resilin is an important elastomeric protein of insects, with roles in the storage and release of energy during a variety of different functional categories including flight and jumping. To date, resilin genes and protein function have been characterised only in a small number of flying insects, despite their importanc...

    journal_title：Insect biochemistry and molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.ibmb.2011.08.002

    authors： Lyons RE,Wong DC,Kim M,Lekieffre N,Huson MG,Vuocolo T,Merritt DJ,Nairn KM,Dudek DM,Colgrave ML,Elvin CM

    更新日期：2011-11-01 00:00:00

  • Biotin-containing proteins of the insect nervous system, a potential source of interference with immunocytochemical localization procedures.

    abstract：:When the biotinylated Manduca sexta adipokinetic hormone gene was used as a probe for in situ hybridization, the intrinsic neurosecretory cells were stained with a biotin detection system that contained streptavidin or avidin. Further experiments showed that the DNA probe was not necessary for staining these cells by ...

    journal_title：Insect biochemistry and molecular biology

    pub_type： 杂志文章

    doi：10.1016/0965-1748(94)00095-y

    authors： Ziegler R,Engler DL,Davis NT

    更新日期：1995-05-01 00:00:00

  • Prostaglandin E2 in the salivary glands of the female tick, Amblyomma americanum (L.): calcium mobilization and exocytosis.

    abstract：:A cholera toxin-sensitive, prostaglandin E2 (PGE2) specific receptor has been identified in the plasma membrane fraction of tick salivary glands. In the present study, we report that stimulation of dispersed salivary glands of the lone star tick Amblyomma americanum (L.) with 1 nM to 10 microM PGE2 increased the intra...

    journal_title：Insect biochemistry and molecular biology

    pub_type： 杂志文章

    doi：10.1016/s0965-1748(98)00018-6

    authors： Qian Y,Yuan J,Essenberg RC,Bowman AS,Shook AL,Dillwith JW,Sauer JR

    更新日期：1998-04-01 00:00:00

  • Molecular cloning of a multidomain cysteine protease and protease inhibitor precursor gene from the tobacco hornworm (Manduca sexta) and functional expression of the cathepsin F-like cysteine protease domain.

    abstract：:A Manduca sexta (tobacco hornworm) cysteine protease inhibitor, MsCPI, purified from larval hemolymph has an apparent molecular mass of 11.5 kDa, whereas the size of the mRNA is very large (∼9 kilobases). MsCPI cDNA consists of a 9,273 nucleotides that encode a polypeptide of 2,676 amino acids, which includes nine tan...

    journal_title：Insect biochemistry and molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.ibmb.2010.08.003

    authors： Miyaji T,Murayama S,Kouzuma Y,Kimura N,Kanost MR,Kramer KJ,Yonekura M

    更新日期：2010-12-01 00:00:00

  • Isolation and characterization of two digestive trypsin-like proteinases from larvae of the stalk corn borer, Sesamia nonagrioides.

    abstract：:Two digestive trypsin-like proteinases from Sesamia nonagrioides Lef. (Lepidoptera: Noctuidae) larvae were purified by benzamidine-Sepharose affinity chromatography. The purified enzymes showed molecular size of 27 (trypsin-I) and 24 KDa (trypsin-II). Amino acid analysis and N-terminal sequencing confirmed their relat...

    journal_title：Insect biochemistry and molecular biology

    pub_type： 杂志文章

    doi：10.1016/s0965-1748(98)00121-0

    authors： Novillo C,Castañera P,Ortego F

    更新日期：1999-02-01 00:00:00

  • Molecular cloning and induction of nuclear receptors from insect cell lines.

    abstract：:Fragments of EcR and USP were cloned from two insect cell lines, Sf21 and High Five cells (derived respectively from Spodoptera frugiperda and Trichoplusia ni), using a PCR-based approach employing degenerate primers designed on the basis of conserved regions of nuclear receptors, together with 5'- and 3'-RACE. An add...

    journal_title：Insect biochemistry and molecular biology

    pub_type： 杂志文章

    doi：10.1016/s0965-1748(01)00144-8

    authors： Chen JH,Turner PC,Rees HH

    更新日期：2002-06-01 00:00:00

  • Genetic analysis by DNA fingerprinting in tsetse fly genomes.

    abstract：:Genomic DNA from tsetse flies (Diptera: Glossinidae: Glossina Wiedemann) was analyzed by hybridization using the whole M13 phage as a probe to reveal DNA fingerprinting (DNAfp) profiles. Intrapopulation variability, measured by comparison of DNAfp profiles of tsetse flies from a large colony of G. brevipalpis, showed ...

    journal_title：Insect biochemistry and molecular biology

    pub_type： 杂志文章

    doi：10.1016/0965-1748(93)90111-5

    authors： Blanchetot A,Gooding RH

    更新日期：1993-12-01 00:00:00

  • Identification of life-stage and tissue-specific splice variants of an inward rectifying potassium (Kir) channel in the yellow fever mosquito Aedes aegypti.

    abstract：:Inward-rectifier potassium (Kir) channels play key roles in nerve, muscle, and epithelial cells in mammals, but their physiological roles in insects remain to be determined. The yellow fever mosquito (Aedes aegypti) possesses five different genes encoding Kir channel subunits: Kir1, Kir2A, Kir2B, Kir2B', and Kir3. We ...

    journal_title：Insect biochemistry and molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.ibmb.2014.03.003

    authors： Rouhier MF,Piermarini PM

    更新日期：2014-05-01 00:00:00

  • Characterization of a juvenile hormone-regulated chymotrypsin-like serine protease gene in Aedes aegypti mosquito.

    abstract：:After female mosquitoes ingest blood from vertebrate hosts, exopeptidases and endopeptidases are required for digesting blood proteins in the midgut into amino acids, which female mosquitoes use to build yolk proteins. These proteases are not always present in the midgut, and their diverse expression patterns suggest ...

    journal_title：Insect biochemistry and molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.ibmb.2007.10.008

    authors： Bian G,Raikhel AS,Zhu J

    更新日期：2008-02-01 00:00:00

  • Aedes aegypti alkaline phosphatase ALP1 is a functional receptor of Bacillus thuringiensis Cry4Ba and Cry11Aa toxins.

    abstract：:Bacillus thuringiensis subs. israelensis produces at least three Cry toxins (Cry4Aa, Cry4Ba, and Cry11Aa) that are active against Aedes aegypti larvae. Previous work characterized a GPI-anchored alkaline phosphatase (ALP1) as a Cry11Aa binding molecule from the gut of A. aegypti larvae. We show here that Cry4Ba binds ...

    journal_title：Insect biochemistry and molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.ibmb.2012.06.001

    authors： Jiménez AI,Reyes EZ,Cancino-Rodezno A,Bedoya-Pérez LP,Caballero-Flores GG,Muriel-Millan LF,Likitvivatanavong S,Gill SS,Bravo A,Soberón M

    更新日期：2012-09-01 00:00:00

  • Infection density of Wolbachia and incompatibility level in two planthopper species, Laodelphax striatellus and Sogatella furcifera.

    abstract：:Wolbachia, a bacterial endosymbiote of arthropods, causes cytoplasmic incompatibility (CI) in many insect species. CI traits were studied in two planthopper species, Laodelphax striatellus and Sogatella furcifera, and Wolbachia densities in these planthopper species were calculated by quantitative PCR methods. The CI ...

    journal_title：Insect biochemistry and molecular biology

    pub_type： 杂志文章

    doi：10.1016/s0965-1748(00)00180-6

    authors： Noda H,Koizumi Y,Zhang Q,Deng K

    更新日期：2001-04-27 00:00:00

  • LmCht5-1 promotes pro-nymphal molting during locust embryonic development.

    abstract：:Chitinases, key enzymes involved in degradation of chitin, have been repeatedly shown to play an indispensable role during insect post-embryonic molting processes at stage transitions. However, how chitinases affect insect embryonic development remains to be analyzed. In this study, we investigated the role of chitina...

    journal_title：Insect biochemistry and molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.ibmb.2018.09.001

    authors： Zhang T,Liu W,Li D,Gao L,Ma E,Zhu KY,Moussian B,Li S,Zhang J

    更新日期：2018-10-01 00:00:00

  • Molecular characterization of neuropeptide elevenin and two elevenin receptors, IsElevR1 and IsElevR2, from the blacklegged tick, Ixodes scapularis.

    abstract：:Understanding salivation in hematophagous arthropod vectors is crucial to developing novel methods to prevent vector-borne disease transmission. The interactions between the tick, host, and pathogens during salivation are highly complex, and are dynamically regulated by the tick central nervous system (synganglion). R...

    journal_title：Insect biochemistry and molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.ibmb.2018.07.005

    authors： Kim D,Šimo L,Park Y

    更新日期：2018-10-01 00:00:00

  • A diverse family of serine proteinase genes expressed in cotton boll weevil (Anthonomus grandis): implications for the design of pest-resistant transgenic cotton plants.

    abstract：:Fourteen different cDNA fragments encoding serine proteinases were isolated by reverse transcription-PCR from cotton boll weevil (Anthonomus grandis) larvae. A large diversity between the sequences was observed, with a mean pairwise identity of 22% in the amino acid sequence. The cDNAs encompassed 11 trypsin-like sequ...

    journal_title：Insect biochemistry and molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.ibmb.2004.06.001

    authors： Oliveira-Neto OB,Batista JA,Rigden DJ,Fragoso RR,Silva RO,Gomes EA,Franco OL,Dias SC,Cordeiro CM,Monnerat RG,Grossi-De-Sá MF

    更新日期：2004-09-01 00:00:00

  • Tripeptides derived from reactive centre loop of potato type II protease inhibitors preferentially inhibit midgut proteases of Helicoverpa armigera.

    abstract：:Potato type II protease inhibitors (Pin-II PIs) impede the growth of lepidopteran insects by inhibiting serine protease-like enzymes in the larval gut. The three amino acid reactive centre loop (RCL) of these proteinaceous inhibitors is crucial for protease binding and is conserved across the Pin-II family. However, t...

    journal_title：Insect biochemistry and molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.ibmb.2018.02.001

    authors： Saikhedkar NS,Joshi RS,Bhoite AS,Mohandasan R,Yadav AK,Fernandes M,Kulkarni KA,Giri AP

    更新日期：2018-04-01 00:00:00

  • cDNA sequences of two biliproteins, BP1 and BP2, from the cabbage white butterfly, Pieris rapae and their tissue- and stage-specific accumulation.

    abstract：:Two similar full-length cDNAs of biliprotein were isolated and shown to encode the two isoelectric forms of biliverdin-binding proteins (BPs): BP1 and BP2 in Pieris rapae. Sequence analysis of two cDNA clones shows that both BPs contain a 567-bp open reading frame which predicts a 189-amino acid protein and a 15-amino...

    journal_title：Insect biochemistry and molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.ibmb.2005.10.006

    authors： Kim HJ,Yun CY,Han YS,Lee IH,Kang YJ,Jin BR,Seo SJ

    更新日期：2006-01-01 00:00:00

  • PCR differential display of immune gene expression in Trichoplusia ni.

    abstract：:The immune state of insects is defined by a set of proteins that is absent in the naive state. To explore the immune system of Trichoplusia ni in more detail we have employed a PCR differential display technique to compare the mRNA population of untreated last instar larvae to that of immunized animals. In the primary...

    journal_title：Insect biochemistry and molecular biology

    pub_type： 杂志文章

    doi：10.1016/0965-1748(95)00080-1

    authors： Kang D,Liu G,Gunne H,Steiner H

    更新日期：1996-02-01 00:00:00

  • An ungrouped cuticular protein is essential for normal endocuticle formation in the brown planthopper.

    abstract：:Using transcriptome analysis of tissues of the brown planthopper (BPH), Nilaparvata lugens, we identified a gene tentatively designated NlCP21.92 that was expressed at high levels in the integument. Spatiotemporal expression profiling with quantitative PCR and Western blotting verified its integument-specific expressi...

    journal_title：Insect biochemistry and molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.ibmb.2018.06.001

    authors： Lu JB,Luo XM,Zhang XY,Pan PL,Zhang CX

    更新日期：2018-09-01 00:00:00

  • Recent advances in understanding mechanisms of insect cuticle differentiation.

    abstract：:Insects possess a cuticle that covers all tissues exposed to the outside world including the body, the fore- and hindgut and the luminal side of the tracheae. The cuticle is a multifunctional device that protects its carriers against dehydration, arms them against predators, constitutes a physical barrier to prevent p...

    journal_title：Insect biochemistry and molecular biology

    pub_type： 杂志文章,评审

    doi：10.1016/j.ibmb.2010.03.003

    authors： Moussian B

    更新日期：2010-05-01 00:00:00

  • Comparative analysis of proteinase activities of Bacillus thuringiensis-resistant and -susceptible Ostrinia nubilalis (Lepidoptera: Crambidae).

    abstract：:Proteinase activities were compared in soluble and membrane fractions of guts obtained from larvae of Bacillus thuringiensis-resistant and -susceptible Ostrinia nubilalis. Overall, serine proteinases from soluble fractions of the susceptible strain were more active than those of the resistant strain. The soluble tryps...

    journal_title：Insect biochemistry and molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.ibmb.2004.03.010

    authors： Li H,Oppert B,Higgins RA,Huang F,Zhu KY,Buschman LL

    更新日期：2004-08-01 00:00:00

  • Molecular characterization of the VLDL receptor homolog mediating binding of lipophorin in oocyte of the mosquito Aedes aegypti.

    abstract：:Lipophorin (Lp) functions as a yolk protein precursor in the mosquito Aedes aegypti and it is internalized via receptor-mediated endocytosis (Insect Biochem. Mol. Biol., 30 (2000) 1161). We cloned and molecularly characterized a putative mosquito ovarian lipophorin receptor (AaLpRov) cDNA. The cDNA has a length of 346...

    journal_title：Insect biochemistry and molecular biology

    pub_type： 杂志文章

    doi：10.1016/s0965-1748(01)00068-6

    authors： Cheon HM,Seo SJ,Sun J,Sappington TW,Raikhel AS

    更新日期：2001-06-22 00:00:00

  • Purification and characterization of the plasma membrane glycosidases of Drosophila melanogaster spermatozoa.

    abstract：:Previous studies from our laboratory have demonstrated the presence of two integral proteins with glycosidase activity in the plasma membrane of Drosophila melanogaster spermatozoa and we have suggested that these enzymes might have a role in sperm-egg binding. In this study the glycosidases have been purified and cha...

    journal_title：Insect biochemistry and molecular biology

    pub_type： 杂志文章

    doi：10.1016/s0965-1748(02)00031-0

    authors： Cattaneo F,Ogiso M,Hoshi M,Perotti ME,Pasini ME

    更新日期：2002-08-01 00:00:00

  • Involvement of PI3K/Akt signaling in PTTH-stimulated ecdysteroidogenesis by prothoracic glands of the silkworm, Bombyx mori.

    abstract：:The prothoracicotropic hormone (PTTH) stimulates ecdysteroidogenesis by prothoracic gland in larval insects. Previous studies showed that Ca(2+), cAMP, extracellular signal-regulated kinase (ERK), and tyrosine kinase are involved in PTTH-stimulated ecdysteroidogenesis by the prothoracic glands of both Bombyx mori and ...

    journal_title：Insect biochemistry and molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.ibmb.2010.12.004

    authors： Gu SH,Young SC,Lin JL,Lin PL

    更新日期：2011-03-01 00:00:00