Abstract：
:We have previously cloned and characterized BmJHE, a juvenile hormone (JH)-selective esterase (JHE) that is important for JH titer regulation in the silkworm Bombyx mori. Here, we sought to determine whether multiple genes might function as JH-specific esterase in this species. We searched for putative carboxyl/cholinesterase (CCE) genes having GQSAG, a highly conserved motif in JHE, by the use of silkworm genomic database. Five novel CCE genes (Bmcce-1-5) were identified and their cDNA sequences and intron-exon structures were determined. We investigated the developmental expression patterns of these CCE genes by real-time quantitative PCR analysis and found that their expression patterns varied among developmental stages and organs. Of the proteins produced by the five genes, only BmCCE-5 had the ability to degrade JH; however, this protein might not function as a JH-specific esterase in vivo as it had a high K(m) value for JH. On the other hand, BmCCE-5 degraded general esterase substrates efficiently. Since Bmcce-5 was strongly expressed in Malpighian tubules and the gut, it might function in digestion or xenobiotic metabolism. Our results suggest that of the CCEs containing a GQSAG motif only BmJHE can function as a JH-specific degradation enzyme in the silkworm.
journal_name
Insect Biochem Mol Bioljournal_title
Insect biochemistry and molecular biologyauthors
Tsubota T,Shimomura M,Ogura T,Seino A,Nakakura T,Mita K,Shinoda T,Shiotsuki Tdoi
10.1016/j.ibmb.2009.12.015subject
Has Abstractpub_date
2010-02-01 00:00:00pages
100-12issue
2eissn
0965-1748issn
1879-0240pii
S0965-1748(09)00195-7journal_volume
40pub_type
杂志文章abstract：:The phytophagous stink bug, Nezara viridula (L.) infests multiple plant species and impacts agricultural production worldwide. We analyzed the transcriptomes of N. viridula accessory salivary gland (ASG), principal salivary gland (PSG) and gut, with a focus on putative digestive proteases and nucleases that present a ...
journal_title：Insect biochemistry and molecular biology
pub_type： 杂志文章
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pub_type： 杂志文章,评审
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journal_title：Insect biochemistry and molecular biology
pub_type： 杂志文章
doi：10.1016/j.ibmb.2007.04.010
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journal_title：Insect biochemistry and molecular biology
pub_type： 杂志文章
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journal_title：Insect biochemistry and molecular biology
pub_type： 杂志文章
doi：10.1016/j.ibmb.2013.10.003
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journal_title：Insect biochemistry and molecular biology
pub_type： 杂志文章
doi：10.1016/s0965-1748(97)00071-4
更新日期：1997-11-01 00:00:00
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journal_title：Insect biochemistry and molecular biology
pub_type： 杂志文章
doi：10.1016/0965-1748(94)90009-4
更新日期：1994-03-01 00:00:00
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journal_title：Insect biochemistry and molecular biology
pub_type： 杂志文章
doi：10.1016/j.ibmb.2017.07.005
更新日期：2017-09-01 00:00:00
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journal_title：Insect biochemistry and molecular biology
pub_type： 杂志文章,评审
doi：10.1016/j.ibmb.2004.03.012
更新日期：2004-07-01 00:00:00
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journal_title：Insect biochemistry and molecular biology
pub_type： 杂志文章
doi：10.1016/j.ibmb.2006.08.005
更新日期：2006-11-01 00:00:00
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pub_type： 杂志文章
doi：10.1016/j.ibmb.2009.09.004
更新日期：2009-11-01 00:00:00
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journal_title：Insect biochemistry and molecular biology
pub_type： 杂志文章
doi：10.1016/j.ibmb.2015.04.002
更新日期：2015-06-01 00:00:00
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journal_title：Insect biochemistry and molecular biology
pub_type： 杂志文章
doi：10.1016/j.ibmb.2019.103179
更新日期：2019-08-01 00:00:00
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journal_title：Insect biochemistry and molecular biology
pub_type： 杂志文章
doi：10.1016/0965-1748(94)00095-y
更新日期：1995-05-01 00:00:00
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journal_title：Insect biochemistry and molecular biology
pub_type： 杂志文章
doi：10.1016/j.ibmb.2015.01.002
更新日期：2015-03-01 00:00:00
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journal_title：Insect biochemistry and molecular biology
pub_type： 杂志文章
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更新日期：2005-11-01 00:00:00
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journal_title：Insect biochemistry and molecular biology
pub_type： 杂志文章
doi：10.1016/j.ibmb.2008.07.002
更新日期：2008-10-01 00:00:00
abstract：:The present investigation is an effort to determine the possible roles of microRNAs (miRNAs) in the regulation of protease gene expression in Helicoverpa armigera upon exposure to plant protease inhibitors (PIs). Using Illumina platform, deep sequencing of 12 small RNA libraries was performed from H. armigera larvae f...
journal_title：Insect biochemistry and molecular biology
pub_type： 杂志文章
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更新日期：2014-11-01 00:00:00
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pub_type： 杂志文章
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更新日期：1997-11-01 00:00:00
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journal_title：Insect biochemistry and molecular biology
pub_type： 杂志文章
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更新日期：2019-06-01 00:00:00
abstract：:The short days of late summer and early fall are the environmental cues that most temperate insects and other animals use to predict winter's arrival. Although it is still unclear precisely how insects measure daylength, there is mounting evidence that the circadian clock regulates seasonal responses including photope...
journal_title：Insect biochemistry and molecular biology
pub_type： 杂志文章
doi：10.1016/j.ibmb.2020.103365
更新日期：2020-06-01 00:00:00
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journal_title：Insect biochemistry and molecular biology
pub_type： 杂志文章
doi：10.1016/j.ibmb.2012.03.004
更新日期：2012-07-01 00:00:00
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journal_title：Insect biochemistry and molecular biology
pub_type： 杂志文章
doi：10.1016/s0965-1748(99)00025-9
更新日期：1999-06-01 00:00:00
abstract：:Phenoloxidases (POs) play key roles in various physiological functions in insects, e.g., cuticular sclerotization, wound healing, egg tanning, cuticle formation and melanotic encapsulaction of pathogens. Previously, we identified five POs, designated As-pro-PO I-V, from the mosquito Armigeres subalbatus and demonstrat...
journal_title：Insect biochemistry and molecular biology
pub_type： 杂志文章
doi：10.1016/j.ibmb.2015.07.016
更新日期：2015-09-01 00:00:00
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journal_title：Insect biochemistry and molecular biology
pub_type： 杂志文章
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更新日期：2019-06-01 00:00:00
abstract：:Two similar full-length cDNAs of biliprotein were isolated and shown to encode the two isoelectric forms of biliverdin-binding proteins (BPs): BP1 and BP2 in Pieris rapae. Sequence analysis of two cDNA clones shows that both BPs contain a 567-bp open reading frame which predicts a 189-amino acid protein and a 15-amino...
journal_title：Insect biochemistry and molecular biology
pub_type： 杂志文章
doi：10.1016/j.ibmb.2005.10.006
更新日期：2006-01-01 00:00:00
