Abstract:
:Malaria transmission by the mosquito vector requires sporozoite invasion into mosquito salivary glands. Parasites probably enter the glands by specific receptor-ligand interactions with molecules on the surface of the glands. We have undertaken the characterization of salivary gland surface molecules of Aedes aegypti to identify candidate receptors for Plasmodium gallinaceum sporozoite invasion. Monoclonal antibodies (mAbs) were generated against antigen enriched for salivary gland membranes and basal lamina. A panel of 44 mAbs were generated that bound to surface molecules of mosquito tissues. Twenty-four mAbs bound exclusively to salivary glands, six bound to salivary glands and ovaries, one bound to salivary gland and midgut, and 13 bound to all tissues tested. We present data on the immunolocalization and biochemical characteristics of the antigens. Many of the salivary gland-specific mAbs bound preferentially to the median and distal lateral lobes of the salivary glands, indicating that there are anatomical region-specific biochemical differences on the gland surface. These lobes of the salivary glands are the preferential sites of malaria sporozoite invasion. Therefore, antigens specific for these regions are promising candidate receptors for sporozoite invasion. The present identification of surface molecules of mosquito salivary glands by means of monoclonal antibodies represents the first description of individual molecules on the mosquito salivary gland surface. This work lays the basis for further studies on the molecular mechanisms involved in malaria sporozoite invasion of mosquito salivary glands.
journal_name
Insect Biochem Mol Bioljournal_title
Insect biochemistry and molecular biologyauthors
Barreau C,Conrad J,Fischer E,Lujan HD,Vernick KDdoi
10.1016/s0965-1748(99)00025-9subject
Has Abstractpub_date
1999-06-01 00:00:00pages
515-26issue
6eissn
0965-1748issn
1879-0240pii
S0965174899000259journal_volume
29pub_type
杂志文章abstract::This analysis considers the structural evidence of a ligand-binding function of the nuclear receptor ultraspiracle (USP). The positions and nature of residues in the ligand-binding domain of USP from six higher insects is evaluated in comparison to the function of conserved residues vertebrate receptors that have been...
journal_title:Insect biochemistry and molecular biology
pub_type: 杂志文章,评审
doi:10.1016/s0965-1748(00)00038-2
更新日期:2000-08-01 00:00:00
abstract::Previous studies from our laboratory have demonstrated the presence of two integral proteins with glycosidase activity in the plasma membrane of Drosophila melanogaster spermatozoa and we have suggested that these enzymes might have a role in sperm-egg binding. In this study the glycosidases have been purified and cha...
journal_title:Insect biochemistry and molecular biology
pub_type: 杂志文章
doi:10.1016/s0965-1748(02)00031-0
更新日期:2002-08-01 00:00:00
abstract::The first required step in ecdysteroid (molting hormone) biosynthesis, dietary cholesterol (C) conversion to 7-dehydrocholesterol (7dC) via 7,8-dehydrogenation, is mediated by a microsomal cytochrome-P450 monooxygenase specific to the larval prothoracic gland. A subsequent series of unknown "black-box" oxidations of 7...
journal_title:Insect biochemistry and molecular biology
pub_type: 杂志文章
doi:10.1016/0965-1748(95)00007-i
更新日期:1995-06-01 00:00:00
abstract::The study of hemolymph melanization in Lepidoptera has contributed greatly to our understanding of its role in insect immunity. Manduca sexta in particular has been an excellent model for identifying the myriad components of the phenoloxidase (PO) cascade and their activation through exposure to pathogen-associated mo...
journal_title:Insect biochemistry and molecular biology
pub_type: 杂志文章
doi:10.1016/j.ibmb.2015.01.002
更新日期:2015-03-01 00:00:00
abstract::When the biotinylated Manduca sexta adipokinetic hormone gene was used as a probe for in situ hybridization, the intrinsic neurosecretory cells were stained with a biotin detection system that contained streptavidin or avidin. Further experiments showed that the DNA probe was not necessary for staining these cells by ...
journal_title:Insect biochemistry and molecular biology
pub_type: 杂志文章
doi:10.1016/0965-1748(94)00095-y
更新日期:1995-05-01 00:00:00
abstract::The three-member Egf gene family from the polydnavirus Microplitis demolitor bracovirus (MdBV) encodes novel proteins distinguished by a shared cysteine-rich motif. Prior studies determined that one family member, Egf1.0, inhibits melanization of hemolymph from the moth Manduca sexta by disabling phenoloxidase activat...
journal_title:Insect biochemistry and molecular biology
pub_type: 杂志文章
doi:10.1016/j.ibmb.2010.04.009
更新日期:2010-07-01 00:00:00
abstract::Molecules in the midgut of the tsetse fly (Diptera: Glossinidiae) are thought to play an important role in the life cycle of African trypanosomes by influencing their initial establishment in the midgut and subsequent differentiation events that ultimately affect parasite transmission. It is thus important to determin...
journal_title:Insect biochemistry and molecular biology
pub_type: 杂志文章
doi:10.1016/s0965-1748(02)00063-2
更新日期:2002-11-01 00:00:00
abstract::The green blowfly species Lucilia cuprina and Lucilia sericata are economically important pests for the sheep industries of Australia and New Zealand. L. cuprina has long been considered a good target for a genetic pest management program. In addition, L. sericata maggots are used in the cleaning of wounds and necroti...
journal_title:Insect biochemistry and molecular biology
pub_type: 杂志文章
doi:10.1016/j.ibmb.2010.09.006
更新日期:2011-01-01 00:00:00
abstract::The immune state of insects is defined by a set of proteins that is absent in the naive state. To explore the immune system of Trichoplusia ni in more detail we have employed a PCR differential display technique to compare the mRNA population of untreated last instar larvae to that of immunized animals. In the primary...
journal_title:Insect biochemistry and molecular biology
pub_type: 杂志文章
doi:10.1016/0965-1748(95)00080-1
更新日期:1996-02-01 00:00:00
abstract::The innate immune system of insects is divided into humoral and cellular defense responses. Humoral defenses include antimicrobial peptides, the cascades that regulate coagulation and melanization of hemolymph, and the production of reactive intermediates of oxygen and nitrogen. Cellular defenses refer to hemocyte-med...
journal_title:Insect biochemistry and molecular biology
pub_type: 杂志文章,评审
doi:10.1016/s0965-1748(02)00092-9
更新日期:2002-10-01 00:00:00
abstract::Inhibition of the binding of [3H]ponasterone A ([3H]PoA) by ecdysone agonists including diacylhydrazines such as RH-5849, tebufenozide (RH-5992) and methoxyfenozide (RH-2485) was examined in intact Drosophila Kc cells. The reciprocal logarithm of the concentration at which there is 50% inhibition of [3H]PoA binding, p...
journal_title:Insect biochemistry and molecular biology
pub_type: 杂志文章
doi:10.1016/s0965-1748(01)00105-9
更新日期:2002-02-01 00:00:00
abstract::Potato synthesises high levels of proteinase inhibitors in response to insect attack. This can adversely affect protein digestion in the insects, leading to reduced growth, delayed development and lowered fecundity. Colorado potato beetle overcomes this defence mechanism by changing the composition of its digestive pr...
journal_title:Insect biochemistry and molecular biology
pub_type: 杂志文章
doi:10.1016/j.ibmb.2004.01.003
更新日期:2004-04-01 00:00:00
abstract::A cholera toxin-sensitive, prostaglandin E2 (PGE2) specific receptor has been identified in the plasma membrane fraction of tick salivary glands. In the present study, we report that stimulation of dispersed salivary glands of the lone star tick Amblyomma americanum (L.) with 1 nM to 10 microM PGE2 increased the intra...
journal_title:Insect biochemistry and molecular biology
pub_type: 杂志文章
doi:10.1016/s0965-1748(98)00018-6
更新日期:1998-04-01 00:00:00
abstract::The increasing resistance level of insect pest species is a major concern to agriculture worldwide. The cotton bollworm, Helicoverpa armigera, is one of the most important pest species due to being highly polyphagous, geographically widespread, and resistant towards many chemical classes of insecticides. We previously...
journal_title:Insect biochemistry and molecular biology
pub_type: 杂志文章
doi:10.1016/j.ibmb.2014.07.006
更新日期:2014-10-01 00:00:00
abstract::Approximately 30-40% of the dry weight of an insect egg consists of lipid, mostly triacylglycerol (TAG). Although this lipid is essential for the energy needed by the developing embryo, little is known about the mechanism that leads to the accumulation of TAG in the insect egg. Insect oocytes can readily synthesize TA...
journal_title:Insect biochemistry and molecular biology
pub_type: 杂志文章,评审
doi:10.1016/j.ibmb.2006.01.014
更新日期:2006-04-01 00:00:00
abstract::Inflammation is an important vertebrate defense mechanism against ecto-parasites for which ticks have evolved numerous mechanisms of modulation. AM-33 and TSGP4, related lipocalins from the soft ticks Argas monolakensis and Ornithodoros savignyi bind cysteinyl leukotrienes with high affinity as measured by isothermal ...
journal_title:Insect biochemistry and molecular biology
pub_type: 杂志文章
doi:10.1016/j.ibmb.2008.06.002
更新日期:2008-09-01 00:00:00
abstract::Flightin was initially identified in Drosophila melanogaster. Previous work has shown that Drosophila flightin plays a key role in indirect flight muscle (IFM) function and has limited expression in the IFM. In this study, we demonstrated that flightin is conserved across the Pancrustacea species, including winged ins...
journal_title:Insect biochemistry and molecular biology
pub_type: 杂志文章
doi:10.1016/j.ibmb.2013.02.006
更新日期:2013-05-01 00:00:00
abstract::A defense mechanism in the hemocytes and cuticle of developing Ceratitis capitata has been demonstrated (Marmaras and Charalambidis, 1992; Marmaras et al., 1993a; Marmaras et al., 1993b). To elucidate further the mechanism and the regulation of defense reactions, we studied this process in relation to melanization in ...
journal_title:Insect biochemistry and molecular biology
pub_type: 杂志文章
doi:10.1016/0965-1748(94)90052-3
更新日期:1994-07-01 00:00:00
abstract::During the screening of immune-regulated genes from the cabbage looper, Trichoplusia ni, a 3-dehydroecdysone 3beta-reductase homologue (DERH) was cloned. In the course of development, 3-dehydroecdysone 3beta-reductase mediates the conversion of 3-dehydroecdysone (3dE) secreted from the prothoracic glands to ecdysone (...
journal_title:Insect biochemistry and molecular biology
pub_type: 杂志文章
doi:10.1016/s0965-1748(01)00145-x
更新日期:2002-08-01 00:00:00
abstract::The biosynthesis of insect juvenile hormone (JH) and its neuroendocrine control are attractive targets for chemical control of insect pests and vectors of disease. To facilitate the molecular study of JH biosynthesis, we analyzed ESTs from the glands producing JH, the corpora allata (CA) in the cockroach Diploptera pu...
journal_title:Insect biochemistry and molecular biology
pub_type: 杂志文章
doi:10.1016/j.ibmb.2006.01.013
更新日期:2006-04-01 00:00:00
abstract::Among disease-vectors, the evolution of the tick innate immune system is still lagging when compared to insects. Such an investigation, which was initiated, by first cloning and sequencing lectins associated in the innate immunity of invertebrates and having fibrinogen related domains, helped in the sequencing of cDNA...
journal_title:Insect biochemistry and molecular biology
pub_type: 杂志文章
doi:10.1016/j.ibmb.2005.04.001
更新日期:2005-09-01 00:00:00
abstract::The major beta-1,3-glucanase from Tenebrio molitor (TLam) was purified to homogeneity (yield, 6%; enrichment, 113 fold; specific activity, 4.4 U/mg). TLam has a molecular weight of 50 kDa and a pH optimum of 6. It is an endoglucanase that hydrolyzes beta-1,3-glucans as laminarin and yeast beta-1,3-1,6-glucan, but is i...
journal_title:Insect biochemistry and molecular biology
pub_type: 杂志文章
doi:10.1016/j.ibmb.2009.10.003
更新日期:2009-12-01 00:00:00
abstract::Eicosanoids play crucial roles in mediating insect immune responses. In vertebrates, phospholipase A2 (PLA2) releases arachidonic acid (AA) from phospholipids (PLs) for biosynthesis of various eicosanoids. However, little AA is found in PLs of lepidopteran insects. Spodoptera exigua, a lepidopteran insect, is known to...
journal_title:Insect biochemistry and molecular biology
pub_type: 杂志文章
doi:10.1016/j.ibmb.2019.103179
更新日期:2019-08-01 00:00:00
abstract::Termites have an important role in the cycling of carbon and trace elements in the biosphere through their degradation of wood, grasses and humus. Glutathione transferases (GSTs) are detoxication enzymes found in all organisms; GSTs are known to protect insects from the toxic effects of plant chemicals and pesticides....
journal_title:Insect biochemistry and molecular biology
pub_type: 杂志文章
doi:10.1016/s0965-1748(96)00019-7
更新日期:1996-06-01 00:00:00
abstract::A novel serine protease, named as scolonase, was purified and characterized from the tissue of the Korean centipede, Scolopendra subspinipes mutilans. Purified scolonase showed an apparent molecular weight of 25 kDa on sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis and an isoelectric point of 4.8 o...
journal_title:Insect biochemistry and molecular biology
pub_type: 杂志文章
doi:10.1016/j.ibmb.2003.10.003
更新日期:2004-03-01 00:00:00
abstract::In moth species that utilize alkenyl sex pheromones, the epoxidation of alkenes confers further diversity on the chemical structures of pheromone components. Hc_epo1 (CYP341B14), the first pheromone gland (PG)-specific epoxidase identified from the fall webworm Hyphantria cunea (Erebidae), specifically epoxidizes the ...
journal_title:Insect biochemistry and molecular biology
pub_type: 杂志文章
doi:10.1016/j.ibmb.2019.03.002
更新日期:2019-05-01 00:00:00
abstract::The first full-length mRNA for vitellogenin (Vg) from ticks was sequenced. This also represents the first complete sequence of Vg from the Chelicerata and of a heme binding Vg. The Vg cDNA from the American dog tick, Dermacentor variabilis was 5744nt in length (GenBank Accession number AY885250), which coded for a pro...
journal_title:Insect biochemistry and molecular biology
pub_type: 杂志文章
doi:10.1016/j.ibmb.2007.01.004
更新日期:2007-04-01 00:00:00
abstract::BhB10-1 is an amplified gene present in DNA puff B10. This gene is very active in the salivary gland regions S1 and S3 at the end of the larval development. Two transcripts of this gene, 1.3 and 1.1 kb in size, were detected. A secretory protein, SP23, is the product of BhB10-1. In this work, we present evidence suppo...
journal_title:Insect biochemistry and molecular biology
pub_type: 杂志文章
doi:10.1016/s0965-1748(01)00156-4
更新日期:2002-07-01 00:00:00
abstract::EFLamide (EFLa) is a neuropeptide known for a long time from crustaceans, chelicerates and myriapods. Recently, EFLa-encoding genes were identified in the genomes of apterygote hexapods including basal insect species. In pterygote insects, however, evidence of EFLa was limited to partial sequences in the bed bug (Cime...
journal_title:Insect biochemistry and molecular biology
pub_type: 杂志文章
doi:10.1016/j.ibmb.2020.103376
更新日期:2020-07-01 00:00:00
abstract::Juvenile hormone (JH) undergoes metabolic degradation by two major pathways involving JH esterase and JH epoxide hydrolase (EH). While considerable effort has been focussed on the study of JH esterase and the development of inhibitors for this enzyme, much less has been reported on the study of JH-EH. In this work, th...
journal_title:Insect biochemistry and molecular biology
pub_type: 杂志文章
doi:10.1016/s0965-1748(00)00048-5
更新日期:2000-08-01 00:00:00