Abstract:
:Among disease-vectors, the evolution of the tick innate immune system is still lagging when compared to insects. Such an investigation, which was initiated, by first cloning and sequencing lectins associated in the innate immunity of invertebrates and having fibrinogen related domains, helped in the sequencing of cDNA encoding for OMFREP from the soft tick, Ornithodoros moubata. Also obtained were Ixoderin A and Ixoderin B cDNA sequences from the hard tick Ixodes ricinus. Tissue-specific expression of OMFREP showed that it was present primarily in the hemocytes and salivary glands. Ixoderin A besides sharing a similar expression profile was also expressed in the midgut. Both showed significantly high homology to the lectin Dorin M, from O. moubata. Further, phylogenetic comparisons between these molecules of the soft and hard ticks showed their relatedness to Tachylectins 5A and 5B, involved in the innate immunity of Tachypleus tridentatus and ficolins from both vertebrates and invertebrates. Ixoderin B showing tissue-specific expression only in the salivary glands and the sequence displaying certain motif differences in homology point towards a possible function different from the other two molecules. This is the first report of lectin-like sequences, with a fibrinogen-domain, from the hard tick I. ricinus and a preliminary phylogenetic study of these tick sequences with related fibrinogen-domain containing sequences highlights a possible role for them in the innate immunity of the ticks.
journal_name
Insect Biochem Mol Bioljournal_title
Insect biochemistry and molecular biologyauthors
Rego RO,Hajdusek O,Kovár V,Kopácek P,Grubhoffer L,Hypsa Vdoi
10.1016/j.ibmb.2005.04.001subject
Has Abstractpub_date
2005-09-01 00:00:00pages
991-1004issue
9eissn
0965-1748issn
1879-0240pii
S0965-1748(05)00101-3journal_volume
35pub_type
杂志文章abstract::During the screening of immune-regulated genes from the cabbage looper, Trichoplusia ni, a 3-dehydroecdysone 3beta-reductase homologue (DERH) was cloned. In the course of development, 3-dehydroecdysone 3beta-reductase mediates the conversion of 3-dehydroecdysone (3dE) secreted from the prothoracic glands to ecdysone (...
journal_title:Insect biochemistry and molecular biology
pub_type: 杂志文章
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abstract::Phenol oxidase (PO) is known to play an important role in defense mechanisms in insect immunity. It is present as a zymogen in insect hemolymph, and can be activated by a specific proteolytic reaction that is stimulated by microbial cell wall components. The pro-phenol oxidase (pro-PO) purified from the larval hemolym...
journal_title:Insect biochemistry and molecular biology
pub_type: 杂志文章
doi:10.1016/s0965-1748(97)00066-0
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journal_title:Insect biochemistry and molecular biology
pub_type: 杂志文章,评审
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更新日期:2002-10-01 00:00:00
abstract::Three laminarinases (LAM, LIC 1, and LIC 2) and two cellulases (CEL 1 and CEL 2) were purified to homogeneity from Periplaneta americana midguts. These beta-glucanases are secreted by salivary glands, stabilized by calcium ions, and have pH optima around 6. LAM (46 kDa) is active only on laminarin, native or with oxid...
journal_title:Insect biochemistry and molecular biology
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abstract::Mosquitoes are responsible for the transmission of numerous human diseases. The recent development of transgenic mosquitoes provides a new tool to examine molecular interactions between insect vectors and the pathogens they transmit. One focus in generating transgenic mosquito lies on expressing anti-pathogenic protei...
journal_title:Insect biochemistry and molecular biology
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journal_title:Insect biochemistry and molecular biology
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journal_title:Insect biochemistry and molecular biology
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doi:10.1016/s0965-1748(00)00009-6
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journal_title:Insect biochemistry and molecular biology
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journal_title:Insect biochemistry and molecular biology
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journal_title:Insect biochemistry and molecular biology
pub_type: 杂志文章
doi:10.1016/j.ibmb.2015.08.002
更新日期:2015-10-01 00:00:00
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journal_title:Insect biochemistry and molecular biology
pub_type: 杂志文章,评审
doi:10.1016/s0965-1748(00)00102-8
更新日期:2001-01-01 00:00:00
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journal_title:Insect biochemistry and molecular biology
pub_type: 杂志文章
doi:10.1016/j.ibmb.2020.103392
更新日期:2020-07-01 00:00:00
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journal_title:Insect biochemistry and molecular biology
pub_type: 杂志文章
doi:10.1016/j.ibmb.2007.12.007
更新日期:2008-05-01 00:00:00
abstract::The Aedes aegypti mosquito is the principal vector for many dangerous human viral diseases. Carbohydrate metabolism (CM) is essential for supplying the energy necessary for host seeking, blood digestion and rapid egg development of this vector insect. The steroid hormone 20-hydroxyecdysone (20E) and the ecdysone recep...
journal_title:Insect biochemistry and molecular biology
pub_type: 杂志文章
doi:10.1016/j.ibmb.2018.02.003
更新日期:2018-05-01 00:00:00
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journal_title:Insect biochemistry and molecular biology
pub_type: 杂志文章
doi:10.1016/j.ibmb.2018.10.001
更新日期:2018-12-01 00:00:00
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journal_title:Insect biochemistry and molecular biology
pub_type: 杂志文章
doi:10.1016/s0965-1748(97)00039-8
更新日期:1997-07-01 00:00:00
abstract::The effect of xenobiotics (phenobarbital and atrazine) on the expression of Drosophila melanogaster CYP genes encoding cytochromes P450, a gene family generally associated with detoxification, was analyzed by DNA microarray hybridization and verified by real-time RT-PCR in adults of both sexes. Only a small subset of ...
journal_title:Insect biochemistry and molecular biology
pub_type: 杂志文章
doi:10.1016/j.ibmb.2006.05.009
更新日期:2006-08-01 00:00:00
abstract::We have previously cloned and characterized BmJHE, a juvenile hormone (JH)-selective esterase (JHE) that is important for JH titer regulation in the silkworm Bombyx mori. Here, we sought to determine whether multiple genes might function as JH-specific esterase in this species. We searched for putative carboxyl/cholin...
journal_title:Insect biochemistry and molecular biology
pub_type: 杂志文章
doi:10.1016/j.ibmb.2009.12.015
更新日期:2010-02-01 00:00:00
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journal_title:Insect biochemistry and molecular biology
pub_type: 杂志文章
doi:10.1016/j.ibmb.2010.08.007
更新日期:2010-12-01 00:00:00
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journal_title:Insect biochemistry and molecular biology
pub_type: 杂志文章
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journal_title:Insect biochemistry and molecular biology
pub_type: 杂志文章
doi:10.1016/j.ibmb.2019.103206
更新日期:2019-09-01 00:00:00
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journal_title:Insect biochemistry and molecular biology
pub_type: 杂志文章
doi:10.1016/s0965-1748(96)00064-1
更新日期:1996-09-01 00:00:00
abstract::There is increasing interest in the development of effective mosquito repellents of natural origin to reduce transmission of diseases such as malaria and yellow fever. To achieve this we have employed an in vitro competition assay involving odorant-binding proteins (OBPs) of the malaria mosquito, Anopheles gambiae, wi...
journal_title:Insect biochemistry and molecular biology
pub_type: 杂志文章
doi:10.1016/j.ibmb.2018.03.008
更新日期:2018-05-01 00:00:00
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journal_title:Insect biochemistry and molecular biology
pub_type: 杂志文章
doi:10.1016/j.ibmb.2007.08.004
更新日期:2007-12-01 00:00:00
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journal_title:Insect biochemistry and molecular biology
pub_type: 杂志文章
doi:10.1016/j.ibmb.2011.12.006
更新日期:2012-04-01 00:00:00
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journal_title:Insect biochemistry and molecular biology
pub_type: 杂志文章
doi:10.1016/j.ibmb.2014.07.006
更新日期:2014-10-01 00:00:00
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journal_title:Insect biochemistry and molecular biology
pub_type: 杂志文章
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journal_title:Insect biochemistry and molecular biology
pub_type: 杂志文章
doi:10.1016/j.ibmb.2010.04.010
更新日期:2010-07-01 00:00:00
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journal_title:Insect biochemistry and molecular biology
pub_type: 杂志文章
doi:10.1016/j.ibmb.2019.103176
更新日期:2019-08-01 00:00:00
abstract::The immune state of insects is defined by a set of proteins that is absent in the naive state. To explore the immune system of Trichoplusia ni in more detail we have employed a PCR differential display technique to compare the mRNA population of untreated last instar larvae to that of immunized animals. In the primary...
journal_title:Insect biochemistry and molecular biology
pub_type: 杂志文章
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