当前位置： SCI文献检索 > INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY期刊下所有文献 > Biosynthetic pathway of arachidonic acid in Spodoptera exigua in response to bacterial challenge.

Biosynthetic pathway of arachidonic acid in Spodoptera exigua in response to bacterial challenge.

Abstract：

:Eicosanoids play crucial roles in mediating insect immune responses. In vertebrates, phospholipase A2 (PLA2) releases arachidonic acid (AA) from phospholipids (PLs) for biosynthesis of various eicosanoids. However, little AA is found in PLs of lepidopteran insects. Spodoptera exigua, a lepidopteran insect, is known to use eicosanoids to mediate immunity. Although AA was not detected in PLs of hemocytes and fat body (two immune tissues) of naïve larvae, it was detected at small but significant level after bacterial infection, suggesting induction of AA biosynthesis for immunity. Based on a mammalian AA biosynthetic pathway, this study hypothesizes that AA is synthesized from C18 polyunsaturated fatty acid (PUFA) precursor by subsequent desaturation and elongation reactions because PLs of S. exigua larvae are rich in linoleic acid. After inhibiting PLA2 activity to prevent release of free fatty acids, different PUFA precursors were injected to S. exigua larvae followed by assessment of eicosanoid-mediated cellular immune response. ω-6 PUFAs were effective in inducing immune response whereas α-linolenic acid (an ω-3 PUFA) was not. Several fatty acyl desaturases (SeDESs) have been predicted from S. exigua transcriptomes. Specific inhibitors against Δ5 or Δ6 DESs inhibited eicosanoid-mediated immune responses. Furthermore, RNA interference (RNAi) specific to Δ5 or Δ6 DES genes significantly suppressed eicosanoid-mediated immune responses. Four very long chain fatty acid elongase genes (SeEloV-A ∼ SeEloV-D) were predicted. Among respective RNAi treatments of these genes, only one RNAi treatment specific to type 5 elongase (SeEloV-B) suppressed eicosanoid-mediated immune response. These results suggest that S. exigua larvae can synthesize AA from linoleic acid via Δ5- and Δ6-desaturations by SeDESs along with chain elongation by SeEloV-B. Finally, this study showed significant fitness cost of uncontrolled AA biosynthesis. AA injection alone without bacterial challenge significantly induced both cellular and humoral immune responses. This unnecessary energy expense due to free AA resulted in reduced pupal size and decreased adult egg production. The detrimental effect of free AA explains physiological significance of little AA content in lepidopteran insects except for life-or-death situation such as pathogen infection.

journal_name

Insect Biochem Mol Biol

journal_title

Insect biochemistry and molecular biology

authors

Hasan MA,Ahmed S,Kim Y

doi

10.1016/j.ibmb.2019.103179

subject

Has Abstract

pub_date

2019-08-01 00:00:00

pages

103179

eissn

0965-1748

issn

1879-0240

pii

S0965-1748(19)30231-0

journal_volume

111

pub_type

杂志文章

相关文献

INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY文献大全
  • Characterization of Kunitz-type protease inhibitor purified from hemolymph of Galleria mellonella larvae.

    abstract：:We characterized a Kunitz-type protease inhibitor (Gm KTPI) obtained from the hemolymph of Galleria mellonella larvae immunized with Escherichia coli. The structural analysis of the cloned cDNA showed that it consists of 56 residues derived from the precursor of 75 amino acids. The peptide was constitutively produced ...

    journal_title：Insect biochemistry and molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.ibmb.2010.08.007

    authors： Lee JH,Kim CH,Shin YP,Park HJ,Park S,Lee HM,Kim BS,Lee IH

    更新日期：2010-12-01 00:00:00

  • The multicopper oxidase gene family in the brown planthopper, Nilaparvata lugens.

    abstract：:The multicopper oxidase (MCO) family of enzymes includes laccases, ascorbate oxidases, bilirubin oxidases and a subgroup of metal oxidases. On the basis of a bioinformatics investigation, we identified 7 genes encoding putative multicopper oxidase proteins in the genome of the brown planthopper (BPH), Nilaparvata luge...

    journal_title：Insect biochemistry and molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.ibmb.2015.06.010

    authors： Ye YX,Pan PL,Kang D,Lu JB,Zhang CX

    更新日期：2015-08-01 00:00:00

  • Mutations of the silkworm molybdenum cofactor sulfurase gene, og, cause translucent larval skin.

    abstract：:Normal silkworms (Bombyx mori) have opaque larval skin due to uric acid accumulation in the epidermis while a mutant, og, is translucent owing to a deficiency in xanthine dehydrogenase (XDH), which synthesizes uric acid. Molybdenum cofactor (MoCo) sulfurase is responsible for XDH activation in various organisms. A sil...

    journal_title：Insect biochemistry and molecular biology

    pub_type： 杂志文章

    doi：10.1016/s0965-1748(03)00006-7

    authors： Kômoto N,Sezutsu H,Yukuhiro K,Banno Y,Fujii H

    更新日期：2003-04-01 00:00:00

  • The ecdysone regulatory cascade and ovarian development in lepidopteran insects: insights from the silkmoth paradigm.

    abstract：:The developing ovariole of the silkmoth Bombyx mori represents an excellent model for studies on the changes that occur in gene expression during the execution of long-term developmental programs. All stages of follicle development, which differ from each other by 2-2.5 h of developmental time, are organized in a sing...

    journal_title：Insect biochemistry and molecular biology

    pub_type： 杂志文章,评审

    doi：10.1016/j.ibmb.2003.06.012

    authors： Swevers L,Iatrou K

    更新日期：2003-12-01 00:00:00

  • Characterization of the Spodoptera frugiperda TATA-binding protein: nucleotide sequence and response to baculovirus infection.

    abstract：:A cDNA clone containing a 921 bp open-reading frame (307 amino acids; 34 kDa) homologous to the TATA-binding protein (TBP) was isolated and sequenced from a Spodoptera frugiperda cell line that is commonly used in the baculovirus expression system. Analysis of the S. frugiperda TBP (SfTBP) sequence showed that the ami...

    journal_title：Insect biochemistry and molecular biology

    pub_type： 杂志文章

    doi：10.1016/0965-1748(94)90057-4

    authors： Rasmussen C,Rohrmann GF

    更新日期：1994-07-01 00:00:00

  • Vasopressin-like peptide and its receptor function in an indirect diuretic signaling pathway in the red flour beetle.

    abstract：:The insect arginine vasopressin-like (AVPL) peptide is of special interest because of its potential function in the regulation of diuresis. Genome sequences of the red flour beetle Tribolium castaneum yielded the genes encoding AVPL and AVPL receptor, whereas the homologous sequences are absent in the genomes of the f...

    journal_title：Insect biochemistry and molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.ibmb.2008.04.006

    authors： Aikins MJ,Schooley DA,Begum K,Detheux M,Beeman RW,Park Y

    更新日期：2008-07-01 00:00:00

  • BmEts upregulates promoter activity of lebocin in Bombyx mori.

    abstract：:The Ets family protein BmEts is assumed to be implicated in determination of diapause in the embryogenesis of Bombyx mori. In this study, we found that expression of BmEts was increased in the fat body and other tissues of the 5th instar larvae in response to Escherichia coli injection. Cotransfection experiments usin...

    journal_title：Insect biochemistry and molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.ibmb.2012.03.004

    authors： Tanaka H,Sagisaka A,Fujita K,Furukawa S,Ishibashi J,Yamakawa M

    更新日期：2012-07-01 00:00:00

  • Identification of life-stage and tissue-specific splice variants of an inward rectifying potassium (Kir) channel in the yellow fever mosquito Aedes aegypti.

    abstract：:Inward-rectifier potassium (Kir) channels play key roles in nerve, muscle, and epithelial cells in mammals, but their physiological roles in insects remain to be determined. The yellow fever mosquito (Aedes aegypti) possesses five different genes encoding Kir channel subunits: Kir1, Kir2A, Kir2B, Kir2B', and Kir3. We ...

    journal_title：Insect biochemistry and molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.ibmb.2014.03.003

    authors： Rouhier MF,Piermarini PM

    更新日期：2014-05-01 00:00:00

  • Action pattern, specificity, lytic activities, and physiological role of five digestive beta-glucanases isolated from Periplaneta americana.

    abstract：:Three laminarinases (LAM, LIC 1, and LIC 2) and two cellulases (CEL 1 and CEL 2) were purified to homogeneity from Periplaneta americana midguts. These beta-glucanases are secreted by salivary glands, stabilized by calcium ions, and have pH optima around 6. LAM (46 kDa) is active only on laminarin, native or with oxid...

    journal_title：Insect biochemistry and molecular biology

    pub_type： 杂志文章

    doi：10.1016/s0965-1748(03)00121-8

    authors： Genta FA,Terra WR,Ferreira C

    更新日期：2003-11-01 00:00:00

  • Comparative transcriptome analysis of chemosensory genes in two sister leaf beetles provides insights into chemosensory speciation.

    abstract：:Divergence in chemosensory traits has been posited as an important component of chemosensory speciation in insects. In particular, chemosensory genes expressed in the peripheral sensory neurons are likely to influence insect behaviors such as preference for food, oviposition sites, and mates. Despite their key role in...

    journal_title：Insect biochemistry and molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.ibmb.2016.11.001

    authors： Zhang B,Zhang W,Nie RE,Li WZ,Segraves KA,Yang XK,Xue HJ

    更新日期：2016-12-01 00:00:00

  • Purification and properties of a beta-glycosidase purified from midgut cells of Spodoptera frugiperda (Lepidoptera) larvae.

    abstract：:Two beta-glycosidases (BG) (Mr 47,000 and Mr 50,000) were purified from Spodoptera frugiperda (Lepidoptera: Noctuidae) midguts. These two polypeptides associate or dissociate depending on the medium ionic strength. The Mr 47,000 BG probably has two active sites. One of the putative active sites (cellobiase site) hydro...

    journal_title：Insect biochemistry and molecular biology

    pub_type： 杂志文章

    doi：10.1016/s0965-1748(00)00090-4

    authors： Marana SR,Terra WR,Ferreira C

    更新日期：2000-12-01 00:00:00

  • The Aedes aegypti glutathione transferase family.

    abstract：:In this report, we describe the glutathione transferase (GST) gene family in the dengue vector Aedes aegypti and suggest a novel role for a new class of mosquito GSTs. Twenty-six GST genes are present in Ae. aegypti, two of which are alternatively spliced to give a total of 29 transcripts for cytosolic GSTs. The six c...

    journal_title：Insect biochemistry and molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.ibmb.2007.05.018

    authors： Lumjuan N,Stevenson BJ,Prapanthadara LA,Somboon P,Brophy PM,Loftus BJ,Severson DW,Ranson H

    更新日期：2007-10-01 00:00:00

  • Transcription factors, CncC and Maf, regulate expression of CYP6BQ genes responsible for deltamethrin resistance in Tribolium castaneum.

    abstract：:Insecticide resistance is a global problem that presents an ongoing challenge to control insects that destroy crops, trees and transmit diseases. Dramatic progress has been made during the last decade on identification of insecticide resistance-associated genes. In one of the most common resistance mechanisms, insects...

    journal_title：Insect biochemistry and molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.ibmb.2015.08.002

    authors： Kalsi M,Palli SR

    更新日期：2015-10-01 00:00:00

  • An immunophilin is a component of the insect ecdysone receptor (EcR) complex.

    abstract：:The ecdysone receptor (EcR) complex has been identified in the prothoracic gland of Manduca sexta by specific immunoprecipitation and Western blot analyses, and includes EcR, ultraspiracle (USP) and FKBP46. The EcR complex binds ponasterone A in a dose-dependent manner with a Kd of 7.04 x 10(-9) M. Immunocytochemistry...

    journal_title：Insect biochemistry and molecular biology

    pub_type： 杂志文章

    doi：10.1016/s0965-1748(97)00080-5

    authors： Song Q,Alnemri ES,Litwack G,Gilbert LI

    更新日期：1997-11-01 00:00:00

  • Constitutive expression and enzymatic activity of Tan protein in brain and epidermis of Ceratitis capitata and of Drosophila melanogaster wild-type and tan mutants.

    abstract：:The present report shows a partial biochemical characterization and life cycle expression of N-β-alanyldopamine hydrolase (Tan protein) in Ceratitis capitata and Drosophila melanogaster. This enzyme catalyzes the hydrolysis of N-β-alanyldopamine (NBAD), the main tanning precursor of insect brown cuticles. It also play...

    journal_title：Insect biochemistry and molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.ibmb.2011.04.007

    authors： Pérez MM,Sabio G,Badaracco A,Quesada-Allué LA

    更新日期：2011-09-01 00:00:00

  • Synthetic rates of key stored fatty acids in the biosynthesis of sex pheromone in the moth Heliothis virescens.

    abstract：:Using a tracer-tracee approach, we fed 1-d-old virgin Heliothis virescens U-(13)C-glucose and analyzed the key labeled fatty acids, (Z)-11-hexadecenoate, hexadecanoate and octadecanoate, known to be intermediates in pheromone biosynthesis, by mass isotopomer distribution analysis. This method allowed determination of ...

    journal_title：Insect biochemistry and molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.ibmb.2012.08.004

    authors： Foster SP,Anderson KG

    更新日期：2012-11-01 00:00:00

  • Infection density of Wolbachia and incompatibility level in two planthopper species, Laodelphax striatellus and Sogatella furcifera.

    abstract：:Wolbachia, a bacterial endosymbiote of arthropods, causes cytoplasmic incompatibility (CI) in many insect species. CI traits were studied in two planthopper species, Laodelphax striatellus and Sogatella furcifera, and Wolbachia densities in these planthopper species were calculated by quantitative PCR methods. The CI ...

    journal_title：Insect biochemistry and molecular biology

    pub_type： 杂志文章

    doi：10.1016/s0965-1748(00)00180-6

    authors： Noda H,Koizumi Y,Zhang Q,Deng K

    更新日期：2001-04-27 00:00:00

  • Molecular sensor of nicotine in taste of Drosophila melanogaster.

    abstract：:Nicotine is an alkaloid and potent parasympathomimetic stimulant found in the leaves of many plants including Nicotiana tabacum, which functions as an anti-herbivore chemical and an insecticide. Chemoreceptors embedded in the gustatory receptor neurons (GRNs) enable animals to judge the quality of bitter compounds and...

    journal_title：Insect biochemistry and molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.ibmb.2019.103178

    authors： Rimal S,Lee Y

    更新日期：2019-08-01 00:00:00

  • Circadian transcription factors differentially regulate features of the adult overwintering diapause in the Northern house mosquito, Culex pipiens.

    abstract：:The short days of late summer and early fall are the environmental cues that most temperate insects and other animals use to predict winter's arrival. Although it is still unclear precisely how insects measure daylength, there is mounting evidence that the circadian clock regulates seasonal responses including photope...

    journal_title：Insect biochemistry and molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.ibmb.2020.103365

    authors： Chang V,Meuti ME

    更新日期：2020-06-01 00:00:00

  • Molecular cloning of trypsin-like cDNAs and comparison of proteinase activities in the salivary glands and gut of the tarnished plant bug Lygus lineolaris (Heteroptera: Miridae).

    abstract：:Using specific proteinase inhibitors, we demonstrated that serine proteinases in the tarnished plant bug, Lygus lineolaris, are major proteinases in both salivary glands and gut tissues. Gut proteinases were less sensitive to inhibition than proteinases from the salivary glands. Up to 80% azocaseinase and 90% of BApNA...

    journal_title：Insect biochemistry and molecular biology

    pub_type： 杂志文章

    doi：10.1016/s0965-1748(03)00094-8

    authors： Zhu YC,Zeng F,Oppert B

    更新日期：2003-09-01 00:00:00

  • HR38, an ortholog of NR4A family nuclear receptors, mediates 20-hydroxyecdysone regulation of carbohydrate metabolism during mosquito reproduction.

    abstract：:The Aedes aegypti mosquito is the principal vector for many dangerous human viral diseases. Carbohydrate metabolism (CM) is essential for supplying the energy necessary for host seeking, blood digestion and rapid egg development of this vector insect. The steroid hormone 20-hydroxyecdysone (20E) and the ecdysone recep...

    journal_title：Insect biochemistry and molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.ibmb.2018.02.003

    authors： Dong D,Zhang Y,Smykal V,Ling L,Raikhel AS

    更新日期：2018-05-01 00:00:00

  • Purification and characterization of NADH-dependent glutamate synthase from the silkworm fat body (Bombyx mori).

    abstract：:NADH-dependent glutamate synthase (Nadh-Gogat; EC 1.41.14) was purified 766-fold from the fat body of 5th instar larvae of the silkworm with a final specific activity of 13.8 units/mg protein by a produce including ammonium sulfate fraction, Q-Sepharose HP ion exchange column chromatography, Blue Sepharose FF affinity...

    journal_title：Insect biochemistry and molecular biology

    pub_type： 杂志文章

    doi：10.1016/s0965-1748(98)00019-8

    authors： Hirayama C,Saito H,Konno K,Shinbo H

    更新日期：1998-07-01 00:00:00

  • Xenobiotic response in Drosophila melanogaster: sex dependence of P450 and GST gene induction.

    abstract：:The effect of xenobiotics (phenobarbital and atrazine) on the expression of Drosophila melanogaster CYP genes encoding cytochromes P450, a gene family generally associated with detoxification, was analyzed by DNA microarray hybridization and verified by real-time RT-PCR in adults of both sexes. Only a small subset of ...

    journal_title：Insect biochemistry and molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.ibmb.2006.05.009

    authors： Le Goff G,Hilliou F,Siegfried BD,Boundy S,Wajnberg E,Sofer L,Audant P,ffrench-Constant RH,Feyereisen R

    更新日期：2006-08-01 00:00:00

  • Juvenile hormone promotes locust fat body cell polyploidization and vitellogenesis by activating the transcription of Cdk6 and E2f1.

    abstract：:Juvenile hormone (JH) is known to promote cell polyploidization for insect vitellogenesis and egg production, but the underlying mechanisms remain poorly understood. Using the migratory locust Locusta migratoria as a model system, we report here that the expression of cyclin-dependent kinase 6 (Cdk6) and adenovirus E2...

    journal_title：Insect biochemistry and molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.ibmb.2018.09.002

    authors： Wu Z,Guo W,Yang L,He Q,Zhou S

    更新日期：2018-11-01 00:00:00

  • Identification of pheromone components and their binding affinity to the odorant binding protein CcapOBP83a-2 of the Mediterranean fruit fly, Ceratitis capitata.

    abstract：:The Mediterranean fruit fly (or medfly), Ceratitis capitata (Wiedemann; Diptera: Tephritidae), is a serious pest of agriculture worldwide, displaying a very wide larval host range with more than 250 different species of fruit and vegetables. Olfaction plays a key role in the invasive potential of this species. Unfortu...

    journal_title：Insect biochemistry and molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.ibmb.2014.02.005

    authors： Siciliano P,He XL,Woodcock C,Pickett JA,Field LM,Birkett MA,Kalinova B,Gomulski LM,Scolari F,Gasperi G,Malacrida AR,Zhou JJ

    更新日期：2014-05-01 00:00:00

  • Glutathione transferases and glutathione-binding proteins of termites: purification and characterisation.

    abstract：:Termites have an important role in the cycling of carbon and trace elements in the biosphere through their degradation of wood, grasses and humus. Glutathione transferases (GSTs) are detoxication enzymes found in all organisms; GSTs are known to protect insects from the toxic effects of plant chemicals and pesticides....

    journal_title：Insect biochemistry and molecular biology

    pub_type： 杂志文章

    doi：10.1016/s0965-1748(96)00019-7

    authors： Haritos VS,Butty JS,Brennan SE,French JR,Ahokas JT

    更新日期：1996-06-01 00:00:00

  • Opsin cDNA sequences of a UV and green rhodopsin of the satyrine butterfly Bicyclus anynana.

    abstract：:The cDNAs of an ultraviolet (UV) and long-wavelength (LW) (green) absorbing rhodopsin of the bush brown Bicyclus anynana were partially identified. The UV sequence, encoding 377 amino acids, is 76-79% identical to the UV sequences of the papilionids Papilio glaucus and Papilio xuthus and the moth Manduca sexta. A dend...

    journal_title：Insect biochemistry and molecular biology

    pub_type： 杂志文章

    doi：10.1016/s0965-1748(02)00058-9

    authors： Vanhoutte KJ,Eggen BJ,Janssen JJ,Stavenga DG

    更新日期：2002-11-01 00:00:00

  • Identification of an aspartylglucosaminidase-like protein in the venom of the parasitic wasp Asobara tabida (Hymenoptera: Braconidae).

    abstract：:This study was designed to identify one of the main components of venomous secretions of the endoparasitic wasp Asobara tabida. By using electrophoretic methods, partial amino acid sequencing and immunostaining, we demonstrated the presence of an aspartylglucosaminidase (AGA)-like protein in the venom of this insect. ...

    journal_title：Insect biochemistry and molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.ibmb.2004.03.001

    authors： Moreau SJ,Cherqui A,Doury G,Dubois F,Fourdrain Y,Sabatier L,Bulet P,Saarela J,Prévost G,Giordanengo P

    更新日期：2004-05-01 00:00:00

  • Identification of cytochrome P450 and glutathione-S-transferase genes preferentially expressed in chemosensory organs of the swallowtail butterfly, Papilio xuthus L.

    abstract：:The swallowtail butterfly, Papilio xuthus L., feeds exclusively on members of the plant family, Rutaceae. Female butterflies lay eggs in response to specific chemicals contained in their host plants. They perceive a variety of polar compounds as oviposition stimulants through the tarsal chemosensilla of the foreleg by...

    journal_title：Insect biochemistry and molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.ibmb.2005.03.013

    authors： Ono H,Ozaki K,Yoshikawa H

    更新日期：2005-08-01 00:00:00

  • Sugar epitopes as potential universal disease transmission blocking targets.

    abstract：:One promising method to prevent vector-borne diseases is through the use of transmission blocking vaccines (TBVs). However, developing several anti-pathogen TBVs may be impractical. In this study, we have identified a conserved candidate carbohydrate target in the midguts of several Arthropod vectors. A screen of the ...

    journal_title：Insect biochemistry and molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.ibmb.2004.09.005

    authors： Dinglasan RR,Valenzuela JG,Azad AF

    更新日期：2005-01-01 00:00:00