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High-throughput detection of knockdown resistance in Myzus persicae using allelic discriminating quantitative PCR.

Abstract：

:The peach-potato aphid Myzus persicae (Sulzer) has developed resistance to pyrethroid insecticides as a result of a mechanism conferring reduced nervous system sensitivity, termed knockdown resistance (kdr). This reduced sensitivity is caused by two mutations, L1014F (kdr) and M918T (super-kdr), in the para-type voltage-gated sodium channel. We have developed a diagnostic dose bioassay to detect kdr and provide preliminary information on the genotype present. We also developed two allelic discrimination PCR assays to determine precisely the genotypes of the two mutations (L1014F and M918T) in individual M. persicae using fluorescent Taqman MGB probes. In combination with assays for elevated carboxylesterase levels and modified acetylcholinesterase (MACE), this suite of assays allows for rapid high-throughput diagnosis, in individual aphids, of the three main resistance mechanisms of practical importance in the UK.

journal_name

Insect Biochem Mol Biol

journal_title

Insect biochemistry and molecular biology

authors

Anstead JA,Williamson MS,Eleftherianos I,Denholm I

doi

10.1016/j.ibmb.2004.06.002

subject

Has Abstract

pub_date

2004-08-01 00:00:00

pages

871-7

issue

8

eissn

0965-1748

issn

1879-0240

pii

S096517480400089X

journal_volume

34

pub_type

杂志文章

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