A water-specific aquaporin involved in aphid osmoregulation.


:The osmotic pressure of plant phloem sap is generally higher than that of insect body fluids. Water cycling from the distal to proximal regions of the gut is believed to contribute to the osmoregulation of aphids and other phloem-feeding insects, with the high flux of water mediated by a membrane-associated aquaporin. A putative aquaporin referred to as ApAQP1 was identified by RT-PCR of RNA isolated from the guts of pea aphids Acyrthosiphon pisum. The ApAQP1 protein has a predicted molecular mass 28.94kDa. Molecular modeling suggests that ApAQP1 has the general aquaporin topology and possesses the conserved pore properties of water-specific aquaporins. When expressed in Xenopus oocytes, ApAQP1 showed the hallmarks of aquaporin-mediated water transport, including an 18-fold increase in the osmotic water permeability of the oolemma, a reduced activation energy, and inhibition of elevated water transport activity by Hg ions. The ApAQP1 transcript was localised to the stomach and distal intestine, and RNAi-mediated knockdown of its expression resulted in elevated osmotic pressure of the haemolymph. Taken together, these data suggest that ApAQP1 contributes to the molecular basis of water cycling in the aphid gut.


Shakesby AJ,Wallace IS,Isaacs HV,Pritchard J,Roberts DM,Douglas AE




Has Abstract


2009-01-01 00:00:00














  • Identification and mapping of the promoter for the gene encoding the ferritin heavy-chain homologue of the yellow fever mosquito Aedes aegypti.

    abstract::Mosquitoes are responsible for the transmission of numerous human diseases. The recent development of transgenic mosquitoes provides a new tool to examine molecular interactions between insect vectors and the pathogens they transmit. One focus in generating transgenic mosquito lies on expressing anti-pathogenic protei...

    journal_title:Insect biochemistry and molecular biology

    pub_type: 杂志文章


    authors: Pham DQ,Shaffer JJ,Chavez CA,Douglass PL

    更新日期:2003-01-01 00:00:00

  • Molecular sensor of nicotine in taste of Drosophila melanogaster.

    abstract::Nicotine is an alkaloid and potent parasympathomimetic stimulant found in the leaves of many plants including Nicotiana tabacum, which functions as an anti-herbivore chemical and an insecticide. Chemoreceptors embedded in the gustatory receptor neurons (GRNs) enable animals to judge the quality of bitter compounds and...

    journal_title:Insect biochemistry and molecular biology

    pub_type: 杂志文章


    authors: Rimal S,Lee Y

    更新日期:2019-08-01 00:00:00

  • Abamectin resistance in Drosophila is related to increased expression of P-glycoprotein via the dEGFR and dAkt pathways.

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    journal_title:Insect biochemistry and molecular biology

    pub_type: 杂志文章


    authors: Luo L,Sun YJ,Wu YJ

    更新日期:2013-08-01 00:00:00

  • cDNA sequences of two biliproteins, BP1 and BP2, from the cabbage white butterfly, Pieris rapae and their tissue- and stage-specific accumulation.

    abstract::Two similar full-length cDNAs of biliprotein were isolated and shown to encode the two isoelectric forms of biliverdin-binding proteins (BPs): BP1 and BP2 in Pieris rapae. Sequence analysis of two cDNA clones shows that both BPs contain a 567-bp open reading frame which predicts a 189-amino acid protein and a 15-amino...

    journal_title:Insect biochemistry and molecular biology

    pub_type: 杂志文章


    authors: Kim HJ,Yun CY,Han YS,Lee IH,Kang YJ,Jin BR,Seo SJ

    更新日期:2006-01-01 00:00:00

  • Purification and cDNA cloning of evolutionally conserved larval cuticle proteins of the silkworm, Bombyx mori.

    abstract::A specific set of structural proteins termed larval cuticle proteins (LCPs) accumulates in integuments during larval development of the silkworm, Bombyx mori. Two major larval cuticle proteins, LCP17 and LCP22, were purified from the guanidine hydrochloride extract of the larval cuticle, and specific antibodies were r...

    journal_title:Insect biochemistry and molecular biology

    pub_type: 杂志文章


    authors: Nakato H,Takekoshi M,Togawa T,Izumi S,Tomino S

    更新日期:1997-08-01 00:00:00

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    journal_title:Insect biochemistry and molecular biology

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    authors: Dong K,Scott JG

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    journal_title:Insect biochemistry and molecular biology

    pub_type: 杂志文章


    authors: Wei YD,Lee KS,Gui ZZ,Yoon HJ,Kim I,Je YH,Lee SM,Zhang GZ,Guo X,Sohn HD,Jin BR

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  • Iridoid biosynthesis in Chrysomelina larvae: Fat body produces early terpenoid precursors.

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    journal_title:Insect biochemistry and molecular biology

    pub_type: 杂志文章


    authors: Burse A,Schmidt A,Frick S,Kuhn J,Gershenzon J,Boland W

    更新日期:2007-03-01 00:00:00

  • Cloning and characterization of LcEcR: a functional ecdysone receptor from the sheep blowfly Lucilia cuprina.

    abstract::Degenerate oligonucleotides were designed on the basis of conserved amino acid sequences in the DNA binding domains of the ecdysone receptors from Drosophila melanogaster (DmEcR) and Chironomus tentans (CtEcR). Using these oligonucleotides a fragment encoding part of the DNA binding domain of the Lucilia cuprina ecdys...

    journal_title:Insect biochemistry and molecular biology

    pub_type: 杂志文章


    authors: Hannan GN,Hill RJ

    更新日期:1997-06-01 00:00:00

  • Partial characterization of oligosaccharides expressed on midgut microvillar glycoproteins of the mosquito, Anopheles stephensi Liston.

    abstract::Midguts of the malaria-transmitting mosquito, Anopheles stephensi, were homogenized and microvillar membranes prepared by calcium precipitation and differential centrifugation. Oligosaccharides present on the microvillar glycoproteins were identified by lectin blotting before and after in vitro and in situ treatments ...

    journal_title:Insect biochemistry and molecular biology

    pub_type: 杂志文章


    authors: Wilkins S,Billingsley PF

    更新日期:2001-09-01 00:00:00

  • Can BRET-based biosensors be used to characterize G-protein mediated signaling pathways of an insect GPCR, the Schistocerca gregaria CRF-related diuretic hormone receptor?

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    journal_title:Insect biochemistry and molecular biology

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    authors: Lismont E,Verbakel L,Vogel E,Corbisier J,Degroot GN,Verdonck R,Verlinden H,Marchal E,Springael JY,Vanden Broeck J

    更新日期:2020-07-01 00:00:00

  • Molecular cloning and partial characterization of a trypsin-like protein in salivary glands of Lygus hesperus (Hemiptera: Miridae).

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    journal_title:Insect biochemistry and molecular biology

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    authors: Zeng F,Zhu YC,Cohen AC

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    journal_title:Insect biochemistry and molecular biology

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    authors: Bayyareddy K,Andacht TM,Abdullah MA,Adang MJ

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    pub_type: 杂志文章


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    journal_title:Insect biochemistry and molecular biology

    pub_type: 杂志文章


    authors: Mans BJ,Louw AI,Neitz AW

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    journal_title:Insect biochemistry and molecular biology

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    authors: Cariño FA,Koener JF,Plapp FW Jr,Feyereisen R

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    journal_title:Insect biochemistry and molecular biology

    pub_type: 杂志文章


    authors: Brown SE,Howard A,Kasprzak AB,Gordon KH,East PD

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  • Mutations of the silkworm molybdenum cofactor sulfurase gene, og, cause translucent larval skin.

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    journal_title:Insect biochemistry and molecular biology

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    authors: Kômoto N,Sezutsu H,Yukuhiro K,Banno Y,Fujii H

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    journal_title:Insect biochemistry and molecular biology

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    authors: Rückert C,Prasad AN,Garcia-Luna SM,Robison A,Grubaugh ND,Weger-Lucarelli J,Ebel GD

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    journal_title:Insect biochemistry and molecular biology

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    authors: Chang CJ,Wu CP,Lu SC,Chao AL,Ho TH,Yu SM,Chao YC

    更新日期:2012-09-01 00:00:00

  • Resistance to diamide insecticides in diamondback moth, Plutella xylostella (Lepidoptera: Plutellidae) is associated with a mutation in the membrane-spanning domain of the ryanodine receptor.

    abstract::Diamide insecticides such as chlorantraniliprole and flubendiamide are a new class of insecticide that selectively target insect ryanodine receptors (RyR), a distinct class of homo-tetrameric calcium release channels which play a pivotal role in calcium homeostasis in numerous cell types. Resistance to these insectici...

    journal_title:Insect biochemistry and molecular biology

    pub_type: 杂志文章


    authors: Troczka B,Zimmer CT,Elias J,Schorn C,Bass C,Davies TG,Field LM,Williamson MS,Slater R,Nauen R

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    journal_title:Insect biochemistry and molecular biology

    pub_type: 杂志文章


    authors: Li H,Oppert B,Higgins RA,Huang F,Zhu KY,Buschman LL

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  • Bacillus thuringiensis Cry1Ac and Cry1Fa delta-endotoxin binding to a novel 110 kDa aminopeptidase in Heliothis virescens is not N-acetylgalactosamine mediated.

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    journal_title:Insect biochemistry and molecular biology

    pub_type: 杂志文章


    authors: Banks DJ,Jurat-Fuentes JL,Dean DH,Adang MJ

    更新日期:2001-07-26 00:00:00

  • Lipid uptake by insect oocytes.

    abstract::Approximately 30-40% of the dry weight of an insect egg consists of lipid, mostly triacylglycerol (TAG). Although this lipid is essential for the energy needed by the developing embryo, little is known about the mechanism that leads to the accumulation of TAG in the insect egg. Insect oocytes can readily synthesize TA...

    journal_title:Insect biochemistry and molecular biology

    pub_type: 杂志文章,评审


    authors: Ziegler R,Van Antwerpen R

    更新日期:2006-04-01 00:00:00

  • Sequence characterization and expression patterns of defensin and lysozyme encoding genes from the gut of the reduviid bug Triatoma brasiliensis.

    abstract::The cDNAs encoding an intestinal defensin (def1) and lysozyme (lys1) of the reduviid bug Triatoma brasiliensis have been amplified by PCR using specific oligonucleotide primers and 5'- and 3'-RACE, cloned and sequenced. The 576 bp clone has an open reading frame of 282 bp and encodes a pre-prodefensin with 94 amino ac...

    journal_title:Insect biochemistry and molecular biology

    pub_type: 杂志文章


    authors: Araújo CA,Waniek PJ,Stock P,Mayer C,Jansen AM,Schaub GA

    更新日期:2006-07-01 00:00:00

  • Expression of genes encoding proteins involved in ecdysteroidogenesis in the female mosquito, Aedes aegypti.

    abstract::A blood meal induces the ovaries of female Aedes aegypti mosquitoes to produce ecdysteroid hormones that regulate many processes required for egg maturation. Various proteins involved in the intracellular transport and biosynthesis of ecdysteroid precursors have been identified by analysis of Drosophila melanogaster m...

    journal_title:Insect biochemistry and molecular biology

    pub_type: 杂志文章


    authors: Sieglaff DH,Duncan KA,Brown MR

    更新日期:2005-05-01 00:00:00

  • De novo biosynthesis versus sequestration: a network of transport systems supports in iridoid producing leaf beetle larvae both modes of defense.

    abstract::In the larval chrysomelines the de novo synthesis of monoterpenoids (iridoids) is believed to represent the ancestral state in the evolution of chemical defenses. Here we demonstrate that the iridoid producing larvae of Plagiodera versicolora and Phratora laticollis have the potential to sequester precursors from food...

    journal_title:Insect biochemistry and molecular biology

    pub_type: 杂志文章


    authors: Kunert M,Søe A,Bartram S,Discher S,Tolzin-Banasch K,Nie L,David A,Pasteels J,Boland W

    更新日期:2008-10-01 00:00:00

  • A lepidopteran pacifastin member: cloning, gene structure, recombinant production, transcript profiling and in vitro activity.

    abstract::Members of the pacifastin family have been characterized as serine peptidase inhibitors (PI), but their target enzyme(s) are unknown in insects. So far, the structural and biochemical characteristics of pacifastin-like PI have only been studied in locusts. Here we report the molecular identification and functional cha...

    journal_title:Insect biochemistry and molecular biology

    pub_type: 杂志文章


    authors: Breugelmans B,Simonet G,van Hoef V,Van Soest S,Smagghe G,Vanden Broeck J

    更新日期:2009-07-01 00:00:00

  • Ribonucleotide reductase subunits from the yellow fever mosquito, Aedes aegypti: cloning and expression.

    abstract::Ribonucleotide reductase catalyses the de novo synthesis of deoxyribonucleotides. Class I reductases use an iron center to generate a tyrosyl free radical that can initiate formation of the deoxyribonucleotide. These reductases are alpha 2 beta 2 holoenzymes, and the subunits are denoted as R1 and R2. R1 contains the ...

    journal_title:Insect biochemistry and molecular biology

    pub_type: 杂志文章


    authors: Pham DQ,Blachuta BJ,Nichol H,Winzerling JJ

    更新日期:2002-09-01 00:00:00