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Molecular cloning and sequencing of cDNAs encoding insecticidal peptides from the primitive hunting spider, Plectreurys tristis (Simon).

Abstract：

:Plectreurys tristis cephalothorax mRNA was isolated and amplified by PCR using degenerate primers corresponding to reverse translated mature Plt-VI toxin. An oligonucleotide corresponding to a portion of the amplified product was then used to screen a P. tristis cDNA library. The cDNAs from 10 positive clones were sequenced. Eight of these cDNAs corresponded to Plt-VI toxin, one to Plt-XI toxin, and one was very similar to Plt-VIII toxin, with the exception of a single amino acid substitution. Analysis of these cDNAs indicated that these toxins are initially synthesized as prepro-forms which undergo signal cleavage followed by additional processing at both their N- and C-termini to produce the mature products.

journal_name

Insect Biochem Mol Biol

journal_title

Insect biochemistry and molecular biology

authors

Leisy DJ,Mattson JD,Quistad GB,Kramer SJ,Van Beek N,Tsai LW,Enderlin FE,Woodworth AR,Digan ME

doi

10.1016/0965-1748(95)00070-4

subject

Has Abstract

pub_date

1996-05-01 00:00:00

pages

411-7

issue

5

eissn

0965-1748

issn

1879-0240

pii

0965174895000704

journal_volume

26

pub_type

杂志文章

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