Whole-Genome Sequencing to Determine Origin of Multinational Outbreak of Sarocladium kiliense Bloodstream Infections.

Abstract:

:We used whole-genome sequence typing (WGST) to investigate an outbreak of Sarocladium kiliense bloodstream infections (BSI) associated with receipt of contaminated antinausea medication among oncology patients in Colombia and Chile during 2013-2014. Twenty-five outbreak isolates (18 from patients and 7 from medication vials) and 11 control isolates unrelated to this outbreak were subjected to WGST to elucidate a source of infection. All outbreak isolates were nearly indistinguishable (<5 single-nucleotide polymorphisms), and >21,000 single-nucleotide polymorphisms were identified from unrelated control isolates, suggesting a point source for this outbreak. S. kiliense has been previously implicated in healthcare-related infections; however, the lack of available typing methods has precluded the ability to substantiate point sources. WGST for outbreak investigation caused by eukaryotic pathogens without reference genomes or existing genotyping methods enables accurate source identification to guide implementation of appropriate control and prevention measures.

journal_name

Emerg Infect Dis

authors

Etienne KA,Roe CC,Smith RM,Vallabhaneni S,Duarte C,Escadon P,Castaneda E,Gomez BL,de Bedout C,López LF,Salas V,Hederra LM,Fernandez J,Pidal P,Hormazabel JC,Otaiza F,Vannberg FO,Gillece J,Lemmer D,Driebe EM,Engleth

doi

10.3201/eid2203.151193

subject

Has Abstract

pub_date

2016-03-01 00:00:00

pages

476-81

issue

3

eissn

1080-6040

issn

1080-6059

journal_volume

22

pub_type

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