Abstract：
BACKGROUND:Ovarian and endometrial high-grade serous carcinomas (HGSCs) have similar clinical and pathological characteristics; however, exhaustive protein expression profiling of these cancers has yet to be reported. METHODS:We performed protein expression profiling on 14 cases of HGSCs (7 ovarian and 7 endometrial) and 18 endometrioid carcinomas (9 ovarian and 9 endometrial) using iTRAQ-based exhaustive and quantitative protein analysis. RESULTS:We identified 828 tumour-expressed proteins and evaluated the statistical similarity of protein expression profiles between ovarian and endometrial HGSCs using unsupervised hierarchical cluster analysis (P<0.01). Using 45 statistically highly expressed proteins in HGSCs, protein ontology analysis detected two enriched terms and proteins composing each term: IMP2 and MCM2. Immunohistochemical analyses confirmed the higher expression of IMP2 and MCM2 in ovarian and endometrial HGSCs as well as in tubal and peritoneal HGSCs than in endometrioid carcinomas (P<0.01). The knockdown of either IMP2 or MCM2 by siRNA interference significantly decreased the proliferation rate of ovarian HGSC cell line (P<0.01). CONCLUSIONS:We demonstrated the statistical similarity of the protein expression profiles of ovarian and endometrial HGSC beyond the organs. We suggest that increased IMP2 and MCM2 expression may underlie some of the rapid HGSC growth observed clinically.
journal_name
Br J Cancerjournal_title
British journal of cancerauthors
Hiramatsu K,Yoshino K,Serada S,Yoshihara K,Hori Y,Fujimoto M,Matsuzaki S,Egawa-Takata T,Kobayashi E,Ueda Y,Morii E,Enomoto T,Naka T,Kimura Tdoi
10.1038/bjc.2016.27subject
Has Abstractpub_date
2016-03-01 00:00:00pages
554-61issue
5eissn
0007-0920issn
1532-1827pii
bjc201627journal_volume
114pub_type
杂志文章abstract：:HER2 / neu is a potential antigen candidate for immunotherapy because of its correlation to a poor prognosis and high expressions in many kinds of epithelial tumours. Especially in the colorectal carcinomas, the higher expression of HER2 / neu is recognized in metastatic regions as well as in primary sites. Several CT...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1054/bjoc.2000.1547
更新日期：2001-01-05 00:00:00
abstract：:Analysis of data from 1434 children with primary renal tumours revealed 57 who developed bone metastases. Of these, 54 were initially recorded as nephroblastoma. Fifty-two of the 57 cases were reviewed histologically, and only 18 were found to be Wilms' tumours. Twenty-three were classified as "Bone-Metastasizing Rena...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/bjc.1980.163
更新日期：1980-06-01 00:00:00
abstract：:Specimens of formalin-fixed, paraffin-embedded non-small-cell lung carcinomas (NSCLCs; n = 187) were analysed immunohistochemically for expression of cyclin A. The analysis was intended to determine whether cyclin A has additional prognostic value for predicting patients' survival and drug response. Of the 187 NSCLCs,...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/bjc.1997.302
更新日期：1997-01-01 00:00:00
abstract：:The ability of human tumour cells to form colonies in soft agar is enhanced by the presence of autologous phagocytic/adherent cells. We investigated the effect of irradiation on the ability of the adherent cells to support human tumour colony formation. Relatively low doses of irradiation significantly increased the g...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/bjc.1983.249
更新日期：1983-11-01 00:00:00
abstract：:Epidemiological, animal, and cell culture studies have identified boron as a chemopreventative agent in prostate cancer. The present objective was to identify boron-induced changes in the DU-145 human prostate cancer cell line. We show that prolonged exposure to pharmacologically-relevant levels of boric acid, the nat...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/sj.bjc.6603009
更新日期：2006-03-27 00:00:00
abstract：:Forty-two patients with small cell lung cancer were treated with a combination of carboplatin, ifosfamide and etoposide. Vincristine was given on day 14 of each course, the courses being repeated every 28 days for a maximum of six. Thoracic radiotherapy was given 4 weeks after the last course of chemotherapy but no pr...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/bjc.1989.228
更新日期：1989-07-01 00:00:00
abstract：:The results of treating two series of patients with Burkitt's lymphoma are presented in the form of survival curves. The second series shows an improved survival rate which seems to be due to a potentiating effect by potassium iodide on oral cyclophosphamide. So far no reason for this effect has been found. ...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/bjc.1971.6
更新日期：1971-03-01 00:00:00
abstract：:During the COVID-19 era, Chinese hospitals have developed a system that enables vulnerable cancer patients to continue to receive high-quality medical care, optimising their survival whilst protecting them. This includes use of digital quick codes, fever clinics and optimal scheduling. We wish to share our experiences...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/s41416-020-0856-0
更新日期：2020-07-01 00:00:00
abstract：:The molecular events that drive the initiation and progression of ovarian adenocarcinoma are not well defined. We have investigated changes in gene expression in ovarian cancer cell lines compared to an immortalized human ovarian surface epithelial cell line (HOSE) using a cDNA array. We identified 17 genes that were ...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1054/bjoc.2000.1620
更新日期：2001-02-02 00:00:00
abstract：:This study describes the effects of the glucolipid synthase inhibitor P4, (DL-threo-1-phenyl-2-palmitoylamino-3-pyrrolidino-1-propanol ), on various functional and phenotypic parameters of 5T33 murine myeloma cells. Cell recovery was reduced by >85% following incubation of the cells for 3 days in the presence of 4 mic...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/sj.bjc.6690792
更新日期：1999-11-01 00:00:00
abstract：:To examine whether weight loss at presentation influences outcome in patients who received chemotherapy for lung cancer or mesothelioma. Multivariate analysis of prospectively collected data 1994-2001. Data were available for age, gender, performance status, histology, stage, response, toxicity, progression-free and o...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/sj.bjc.6601781
更新日期：2004-05-17 00:00:00
abstract：:The cytochrome P450 CYP1B1 is consistently overexpressed in tumour cells as compared to their normal counterparts, but its precise role in drug resistance is yet to be defined. It has been reported that transfection of CYP1B1 results in increased resistance to docetaxel in V79 cells (McFadyen et al, 2001). In this stu...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/sj.bjc.6604195
更新日期：2008-02-12 00:00:00
abstract：:When L2C leukaemic B lymphocytes from guinea-pigs were incubated in vitro with antibody directed to their surface immunoglobulin (Ig), a rapid rise in intracellular adenosine 3':5'-phosphate (cyclic adenosine monophosphate, cAMP) was observed. Estimation of cAMP was by a protein-binding assay using bovine adrenal prot...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/bjc.1979.77
更新日期：1979-04-01 00:00:00
abstract：BACKGROUND:This study is to investigate the effects of geserelin+tamoxifen (TAM) on estradiol level, breast density (BD), endometrial thickness (ET), and blood lipids in premenopausal and perimenopausal women with hormone receptor-positive early-stage breast cancer. METHODS:This study recruited 110 premenopausal and p...
journal_title：British journal of cancer
pub_type： 杂志文章,随机对照试验
doi：10.1038/bjc.2013.324
更新日期：2013-08-06 00:00:00
abstract：:Comparative genomic hybridization (CGH) was used to detect copy number changes of DNA sequences in the Ewing family of tumours (ET). We analysed 20 samples from 17 patients. Fifteen tumours (75%) showed copy number changes. Gains of DNA sequences were much more frequent than losses, the majority of the gains affecting...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/bjc.1997.242
更新日期：1997-01-01 00:00:00
abstract：BACKGROUND:When tumour tissue is unavailable, cell-free DNA (cfDNA)can serve as a surrogate for genetic analyses. Because mutated alleles in cfDNA are usually below 1%, next-generation sequencing (NGS)must be narrowed to target only clinically relevant genes. In this proof-of-concept study, we developed a panel to use ...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/bjc.2017.8
更新日期：2017-03-14 00:00:00
abstract：:The effect on cell-cycle progression in various phases of the cell cycle caused by an acute exposure to hypoxia in absence and presence of misonidazole (MISO) was investigated. Exponentially growing and synchronized cells of the human line NHIK 3025 were exposed to different degrees of hypoxia for a short period (1.5 ...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/bjc.1983.271
更新日期：1983-12-01 00:00:00
abstract：:The influence of anaesthetics on the in vivo response of B16 melanoma to melphalan was studied using an in vitro cell-survival assay. Three anaesthetics were used, Saffan (Althesin) Sagatal (Nembutal) and Hypnorm. When Saffan was administered to tumour-bearing animals before melphalan there was a significant increase ...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/bjc.1978.279
更新日期：1978-12-01 00:00:00
abstract：:Cell viability following short (1 h) contact with paclitaxel or docetaxel was assayed using synchronised HeLa cells. Docetaxel proved almost totally lethal against S-phase cells. Its toxicity was only partial against cells in mitosis, and declined to a minimum with progression to G1. For paclitaxel, cytotoxicity incre...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/bjc.1995.232
更新日期：1995-06-01 00:00:00
abstract：BACKGROUND:We analysed critically the potential usefulness of RNA- and DNA-based biomarkers in supporting conventional histological diagnostic tests for prostate carcinoma (PCa) detection. METHODS:Microarray profiling of gene expression and DNA methylation was performed on 16 benign prostatic hyperplasia (BPH) and 32 ...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/bjc.2014.337
更新日期：2014-08-12 00:00:00
abstract：:Patients with advanced malignant disease show an apparent lesser degree of lymphocyte sensitization to cancer antigen when tested under standard conditions than do early cases. In the serum of cancer patients there is a lymphocyte response depressing factor whose titre rises as the neoplasm becomes more extensive. The...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/bjc.1972.23
更新日期：1972-06-01 00:00:00
abstract：:The efficacy of radioimmunotherapy of tumours with radiolabelled monoclonal antibodies (MAbs) depends on the amount of antibody taken up by the tumour and on its intratumoral distribution. In the case of MAbs directed against intracellular antigens, increasing the permeability of the cytoplasmic membrane may augment t...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/bjc.1995.468
更新日期：1995-11-01 00:00:00
abstract：BACKGROUND:Renal cell carcinoma (RCC) is highly resistant to chemotherapy because of a high apoptotic threshold. Recent evidences suggest that GSK-3beta positively regulates human pancreatic cancer and leukaemia cell survival in part through regulation of nuclear factor (NF-kappaB)-mediated expression of anti-apoptotic...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/sj.bjc.6605437
更新日期：2009-12-15 00:00:00
abstract：:The present study investigates the role of nitric oxide and the involvement of nitric oxide synthase II isoform on the invasion of human colorectal adenocarcinoma cell lines HRT-18 and HT-29. HRT-18 cells, which constitutively express nitric oxide synthase II mRNA were three-fold more invasive in a Matrigel invasion a...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/sj.bjc.6600224
更新日期：2002-04-22 00:00:00
abstract：:The incidence of nodal metastasis in early gastric carcinoma (EGC) is 10-20%. However, the optimal nodal dissection for early gastric carcinoma has not been established. A retrospective study was conducted in 392 consecutive patients who underwent potentially curative distal gastrectomy for EGC between 1962 and 1990. ...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/bjc.1995.540
更新日期：1995-12-01 00:00:00
abstract：:The nature of the invasive growth pattern and microvessel density (MVD) have been suggested to be predictors of prognosis in primary colorectal cancer (CRC) and colorectal liver metastases. The purpose of the present study was to determine whether these two histological features were interrelated and to assess their r...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/sj.bjc.6603677
更新日期：2007-04-10 00:00:00
abstract：:We studied 5555 seminoma patients and 3733 patients with nonseminomatous testicular cancers diagnosed in Southeast England between 1960 and 2004. For both groups survival improved over time: 10-year relative survival increased from 78% in 1960-1969 to 99% in 1990-2004 for seminomas, and from 55 to 95% for nonseminomas...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/sj.bjc.6603589
更新日期：2007-02-12 00:00:00
abstract：:Antibody-dependent cellular cytotoxicity (ADCC) is considered to be the major mechanism through which tumour cells, upon treatment with anti-tumour MAbs, are eliminated in vivo. However, the relative importance of various parameters that influence the efficacy of ADCC is unclear. Here we present in vitro data on the i...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/bjc.1998.518
更新日期：1998-08-01 00:00:00
abstract：:Insulin-like growth factors (IGFs) I and II are implicated in progression of various tumours including colorectal carcinomas. To interfere with the production of IGFs, we treated male nude mice bearing xenografts of HT-29 human colon cancer with various potent growth hormone-releasing hormone (GH-RH) antagonists. Twic...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1054/bjoc.2000.1223
更新日期：2000-05-01 00:00:00
abstract：:Several techniques are under development to diagnose oesophageal adenocarcinoma at an earlier stage. We have demonstrated the potential of Raman spectroscopy, an optical diagnostic technique, for the identification and classification of malignant changes. However, there is no clear recognition of the biochemical chang...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/sj.bjc.6603102
更新日期：2006-05-22 00:00:00
