Abstract:
BACKGROUND:The combined effects of Human papillomavirus (HPV) and Herpes simplex type 1 (HSV-1) infections and their effects on cancer cell radioresistance are unexplored. MATERIALS AND METHODS:An HPV16-positive hypopharyngeal carcinoma cell line (UD-SCC-2) was infected with wt-HSV-1 at low multiplicity of infection (MOI) and irradiated with 2 Gy at 24 h postinfection. Viability assays and quantitative reverse-transcriptase PCR for HPV16 E6, E7, nuclear factor kappa B1, B-cell CLL/lymphoma 2 (BCL2), and caspases 3, 8 and 9 at 24, and 72 h, as well as immunocytochemistry for BCL2, caspase 3, cyclin E, mouse double minute 2 homolog (MDM2), HSV-1 and Ki-67 were performed at 144 h postirradiation. RESULTS:At 144 h, cell viability was significantly lowered by irradiation only in uninfected cells. Infection combined with irradiation resulted in increased expression of E6, E7, BCL2 and NF-κB1 at 144 h. Simultaneously, E6 and E7 were down-regulated in non-irradiated infected cells. Irradiation and infection with 0.00001 MOI separately up-regulated caspase 3 but infection with 0.0001 MOI halved its expression in irradiated cells. CONCLUSION:HSV-1 infection modulates radioresistance of HPV16-positive hypopharyngeal carcinoma cells.
journal_name
Anticancer Resjournal_title
Anticancer researchauthors
Turunen A,Hukkanen V,Kulmala J,Syrjanen Ssubject
Has Abstractpub_date
2016-02-01 00:00:00pages
565-74issue
2eissn
0250-7005issn
1791-7530pii
36/2/565journal_volume
36pub_type
杂志文章abstract:AIM:To investigate whether there is a difference in selenium levels before and after radiotherapy (RT) and to study the effects of serum selenium levels on RT-related toxicity in patients undergoing RT for head and neck cancer. PATIENTS AND METHODS:A population of 47 consecutive patients was enrolled in the study. RT ...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:2012-08-01 00:00:00
abstract::The cyclin-dependent kinase inhibitor p21WAF1 participates in cell growth, differentiation, and apoptosis. p21WAF1 can be induced by green tea polyphenol EGCG in several cancer cell types, but its role in the oral cancer cell response to EGCG is not known. We found that EGCG upregulates p21WAF1 in an oral carcinoma ce...
journal_title:Anticancer research
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doi:
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journal_title:Anticancer research
pub_type: 杂志文章
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journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:2005-11-01 00:00:00
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journal_title:Anticancer research
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journal_title:Anticancer research
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journal_title:Anticancer research
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doi:
更新日期:2016-03-01 00:00:00
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journal_title:Anticancer research
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journal_title:Anticancer research
pub_type: 杂志文章
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更新日期:2017-09-01 00:00:00
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journal_title:Anticancer research
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doi:
更新日期:2014-02-01 00:00:00
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journal_title:Anticancer research
pub_type: 杂志文章
doi:
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journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:1995-05-01 00:00:00
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journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:1992-05-01 00:00:00
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journal_title:Anticancer research
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doi:
更新日期:1999-01-01 00:00:00
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journal_title:Anticancer research
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doi:
更新日期:2015-05-01 00:00:00
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journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:1995-03-01 00:00:00
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journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:2013-11-01 00:00:00
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journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:2003-07-01 00:00:00
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journal_title:Anticancer research
pub_type: 杂志文章
doi:
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journal_title:Anticancer research
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doi:
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journal_title:Anticancer research
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doi:
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journal_title:Anticancer research
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doi:
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journal_title:Anticancer research
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doi:
更新日期:2010-05-01 00:00:00
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journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:2012-07-01 00:00:00
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journal_title:Anticancer research
pub_type: 杂志文章
doi:
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journal_title:Anticancer research
pub_type: 杂志文章
doi:
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journal_title:Anticancer research
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doi:
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journal_title:Anticancer research
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doi:
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journal_title:Anticancer research
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doi:
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