Abstract:
AIMS:Chronic renal allograft loss is still an unsolved problem in kidney transplantation. We evaluated the impact of FTY720, a S1P receptor agonist, known to deplete lymphocytes from the peripheral blood by sequestering them into lymph nodes and Peyer's patches, on blood lymphocytes and graft infiltrating cells in a rat model of chronic real allograft rejection. METHODS:LEW rats served as recipients for LEW.1U7B kidney grafts. All animals were treated with CsA (5mg/kg) for 10 days after renal transplantation and monitored for kidney function, peripheral blood lymphocytes and graft infiltrating cells. In the intervention group (n=7) FTY720 therapy was started 7 weeks post-KTx in a dose of 0.5 mg/kg p. o. three times a week. RESULTS:In the control group the survival of the rats was 9, 11, 18 and 4 × 24 weeks, in the intervention group 2 × 8, 9, 2 × 11, 18 and 20 weeks. While in the intervention group the number of T- and B-lymphocytes in the peripheral blood was successfully reduced during FTY720 treatment, both groups showed significant amounts of T- and B-lymphocytes in the kidney grafts. Animals in both groups developed donor specific antibodies, extensive albuminuria and severe chronic changes in the grafts. CONCLUSIONS:FTY720 was highly effective to reduce T- and B-lymphocytes in the peripheral blood, but not effective in clearing their infiltration in the graft. Graft survival was not prolonged by FTY720 treatment starting late after kidney transplantation.
journal_name
Transpl Immunoljournal_title
Transplant immunologyauthors
Koch M,Poehnert D,Nashan Bdoi
10.1016/j.trim.2016.01.003subject
Has Abstractpub_date
2016-03-01 00:00:00pages
12-7eissn
0966-3274issn
1878-5492pii
S0966-3274(16)30003-Xjournal_volume
35pub_type
杂志文章abstract::B(io) B(reedding)/O(ttawa) K(alsburg) rats spontaneously develop insulin-dependent type 1 diabetes. Days before BB/OK rats become diabetic, their body seems to be flabby which may be attributed to loss of subcutaneous fat. However, the rats are normoglycemic and manifest 3-4 days later. This observation prompted us to...
journal_title:Transplant immunology
pub_type: 杂志文章
doi:10.1016/j.trim.2011.01.003
更新日期:2011-05-01 00:00:00
abstract::We present management strategies utilised for the first case of an urgent live-donor ABO incompatible B blood group renal transplant, in a patient with a prior A blood group lung transplant for cystic fibrosis. Three years on, renal function is excellent and stable, whilst lung function has improved. ...
journal_title:Transplant immunology
pub_type: 杂志文章
doi:10.1016/j.trim.2016.09.002
更新日期:2016-11-01 00:00:00
abstract:BACKGROUND:Every transplanted organ relies on a reliable and sound vascular system. Therefore, our study focused on the investigation if platelet inhibition alone or combined with mTOR-inhibition has a beneficial effect on the microvascular integrity in allogeneic murine skin grafts. METHODS:Skin transplantation was p...
journal_title:Transplant immunology
pub_type: 杂志文章
doi:10.1016/j.trim.2015.09.005
更新日期:2015-11-01 00:00:00
abstract:BACKGROUND:Xenotransplantation is an appealing alternative to human allotransplantation because of a worldwide shortage of organs. One of the obstacles for xenografts is cellular rejection by the innate immune system, comprised of NK cells, monocytes, and macrophages. In this study the inhibitory function of HLA-G1, a ...
journal_title:Transplant immunology
pub_type: 杂志文章
doi:10.1016/j.trim.2014.12.004
更新日期:2015-03-01 00:00:00
abstract::This study provides data on HLA-A, -B, and -DRB1 frequencies among 861 end-stage renal disease (ESRD) patients from Croatia and estimates the benefit of the kidney exchange program by comparing HLA distribution and assessing HLA mismatches (MMs) within a group of ESRD patients who received kidney grafts from 707 cadav...
journal_title:Transplant immunology
pub_type: 杂志文章
doi:10.1016/j.trim.2020.101318
更新日期:2020-10-01 00:00:00
abstract:BACKGROUND:Results from published studies on the association of donor or recipient IL-6 -174G/C (rs1800795) polymorphism with acute rejection (AR) of renal allograft are conflicting. We performed a meta-analysis to estimate the possible association. METHODS:Studies were identified by searching PUBMED and EMBASE until ...
journal_title:Transplant immunology
pub_type: 杂志文章,meta分析,评审
doi:10.1016/j.trim.2011.10.003
更新日期:2012-01-01 00:00:00
abstract:BACKGROUND:B cell depletion by anti-CD20 antibody is used in desensitization protocols and for treatment of antibody-mediated rejection (AMR). However, little is known about the efficacy and the mechanism(s) of action. METHODS:A mouse model of HLA sensitization was used to study the effectiveness of anti-CD20 treatmen...
journal_title:Transplant immunology
pub_type: 杂志文章
doi:10.1016/j.trim.2008.05.004
更新日期:2008-07-01 00:00:00
abstract::We investigated the dynamics of the CD4+ and CD8+ lymphocyte subsets, and the expression of activation markers in cardiac transplant recipients. We tested 132 peripheral blood samples from 62 cardiac transplant recipients using fluorescent staining and flow cytometry analysis. The results were correlated with histolog...
journal_title:Transplant immunology
pub_type: 杂志文章
doi:10.1016/s0966-3274(02)00072-2
更新日期:2002-11-01 00:00:00
abstract::Adenoviral gene transfer is a potential ex vivo gene therapy for islet transplantation. However, the immunogenicity of adenoviral vectors can potentially impair vector efficacy in transplants where long-term gene expression is required. We investigated the effect of this antiviral immunity on vector expression and isl...
journal_title:Transplant immunology
pub_type: 杂志文章
doi:10.1016/j.trim.2009.02.001
更新日期:2009-05-01 00:00:00
abstract::Models of severe combined immuno-deficient (SCID) mice reconstituted with a competent human immune system represent a valuable tool for the study of human immune responses in vivo. Reconstitution with human cells can be achieved using large numbers of peripheral blood lymphocytes, but levels of engraftment are poor an...
journal_title:Transplant immunology
pub_type: 杂志文章
doi:10.1016/j.trim.2005.07.002
更新日期:2005-12-01 00:00:00
abstract::The neomacrolide antibiotic azithromycin is known to have an anti-inflammatory effect and is increasingly being used in the treatment of chronic inflammatory pulmonary diseases. We investigated whether azithromycin influenced matrix remodeling. Matrix metalloproteinase (MMP)-9 protein levels were measured by ELISA in ...
journal_title:Transplant immunology
pub_type: 杂志文章
doi:10.1016/j.trim.2011.06.006
更新日期:2011-09-01 00:00:00
abstract::Circulating host lymphocytes recognize, bind to, become activated by, and infiltrate engrafted allogeneic tissues. The mechanisms responsible for these early events which lead to acute immunological rejection have not been precisely defined. We have examined sequentially in vitro lymphocyte binding patterns in a kidne...
journal_title:Transplant immunology
pub_type: 杂志文章
doi:10.1016/0966-3274(95)80035-2
更新日期:1995-06-01 00:00:00
abstract::Calcineurin a calmodulin-dependent phosphatase plays a critical role in calcium-dependent activation of T-lymphocytes and is the major target for the inhibitory actions of the immunosuppressive drugs Tacrolimus (FK506) and Cyclosporin A (CsA). Calcineurin is a dimeric protein consisting of distinct A (catalytic) and B...
journal_title:Transplant immunology
pub_type: 杂志文章
doi:10.1016/s0966-3274(00)00020-4
更新日期:2000-06-01 00:00:00
abstract::Adenovirus-mediated CTLA4Ig gene transfer has been reported to enhance graft survival in several rodent transplantation models. In this study, we investigated the efficacy of ex vivo and systemic transfer of the CTLA4Ig gene by adenoviral vectors in pancreatic islet allo-transplantation. Islet grafts from BN rats were...
journal_title:Transplant immunology
pub_type: 杂志文章
doi:10.1016/S0966-3274(02)00153-3
更新日期:2003-01-01 00:00:00
abstract:BACKGROUND:We recently reported that autoreactive antibody (Ab) against nuclear histone H1 had been identified as an immunosuppressive factor in a rat tolerogenic orthotopic liver transplantation (OLT) model. The present study aimed to determine whether the up-regulation of antihistone H1 Ab by histone H1 vaccination l...
journal_title:Transplant immunology
pub_type: 杂志文章
doi:10.1016/j.trim.2007.01.003
更新日期:2007-04-01 00:00:00
abstract::Mouse hearts transplanted into MHC disparate donors are usually rejected 1 week after placement. It is widely accepted that alloantigen-reactive helper T lymphocytes (HTL) and cytotoxic T lymphocytes (CTL) are the key mediators of acute allograft rejection. This report demonstrates that the presence or absence of 'tra...
journal_title:Transplant immunology
pub_type: 杂志文章
doi:10.1016/0966-3274(95)80028-x
更新日期:1995-09-01 00:00:00
abstract::Induction of donor-specific hyporesponsiveness would minimize the need for intensive immunosuppression in the clinical setting of graft rejection and dendritic cells (DCs) might be useful tools for this purpose. Besides their ability to induce immunogenic T-cell responses, these antigen presenting cells can lead to T-...
journal_title:Transplant immunology
pub_type: 杂志文章
doi:10.1016/j.trim.2006.03.001
更新日期:2006-06-01 00:00:00
abstract::Mycophenolate mofetil (MMF) a potent immunosuppressive agent, has recently been approved for clinical use (CellCept) in renal transplant patients in combination with cyclosporine (CsA). With the expanded use of tacrolimus (Prograf) as well in renal transplant patients, there is a lack of pharmacokinetic studies clarif...
journal_title:Transplant immunology
pub_type: 临床试验,杂志文章
doi:10.1016/s0966-3274(97)80042-1
更新日期:1997-09-01 00:00:00
abstract:OBJECTIVE:Acute rejection is a major cause of morbidity and mortality after solid organ transplantation. Therefore, optimizing treatment strategies and improving curative effect is urgent and necessary. Reliable biomarkers for acute rejection and the underlying molecular mechanisms remain to be determined. METHODS:In ...
journal_title:Transplant immunology
pub_type: 杂志文章
doi:10.1016/j.trim.2020.101320
更新日期:2020-10-01 00:00:00
abstract:BACKGROUND AND PURPOSE:Since the Fas/Fas Ligand (FasL) interaction has been recognized as an apoptotic pathway, it eliminates the activated T cells and promotes the survival of grafts. In this study, the effect of FasL transfection of pig chondrocytes on allogeneic transplantation was examined in vitro and in vivo. ME...
journal_title:Transplant immunology
pub_type: 杂志文章
doi:10.1016/j.trim.2008.02.001
更新日期:2008-05-01 00:00:00
abstract:BACKGROUND:Correct identification of the specificity of antibodies directed against HLA using single antigen Luminex beads (SALB) is essential in current HLA laboratory practice for transplantation. The aim of this study was to investigate the magnitude of concordance and discordance among laboratories in testing for a...
journal_title:Transplant immunology
pub_type: 杂志文章,多中心研究
doi:10.1016/j.trim.2014.10.003
更新日期:2015-01-01 00:00:00
abstract::Although natural killer cells lyse targets without pre-sensitization, and in an MHC-unrestricted manner, they can also respond to healthy allogeneic cells of different MHC type. Such alloreactivity is a consequence of NK cells using clonally distributed, inhibitory MHC class I receptors to achieve tolerance to healthy...
journal_title:Transplant immunology
pub_type: 杂志文章,评审
doi:10.1016/j.trim.2006.09.022
更新日期:2006-12-01 00:00:00
abstract::It is known that the expression level of the heat shock protein (HSP) is elevated in allograft tissues, but the specific role of the HSP in the acute rejection has not been elucidated. This study aims to determine how and when the HSP72 molecule works immunologically in the process of acute allograft rejection from a ...
journal_title:Transplant immunology
pub_type: 杂志文章
doi:10.1016/j.trim.2004.08.003
更新日期:2004-12-01 00:00:00
abstract::TIRC7 delivers essential signals during immune activation as antibodies targeting TIRC7 inhibit lymphocyte proliferation and Th1 cytokine expression in vitro and prolonged kidney and heart allograft survival in vivo. Immunohistochemical analysis of biopsy specimens from human renal allografts undergoing rejection desp...
journal_title:Transplant immunology
pub_type: 杂志文章
doi:10.1016/j.trim.2006.09.027
更新日期:2006-11-01 00:00:00
abstract:BACKGROUND:After lung transplantation, the major complication limiting the long-term survival of allografts is obliterative bronchiolitis (OB), characterized by chronic rejection. Innate immune responses contribute to the development of OB. In this study, we used a murine heterotopic tracheal transplantation mouse mode...
journal_title:Transplant immunology
pub_type: 杂志文章
doi:10.1016/j.trim.2018.11.006
更新日期:2019-04-01 00:00:00
abstract:BACKGROUND:Graft-versus-host disease is one of the major complications after allogeneic bone marrow transplantation, but it is not easy to anticipate the onset. Cytokines released by type 1 T helper cells are thought to play a pivotal role in acute graft-versus-host disease aGVHD. The ability to predict the likely occu...
journal_title:Transplant immunology
pub_type: 杂志文章
doi:10.1016/j.trim.2005.10.002
更新日期:2006-01-01 00:00:00
abstract::Immunological monitoring of transplant recipients is an attractive concept. Cytokines provide an obvious focus for research, as they are central to the human immune response. This study aimed to identify cytokines whose sequential gene expression differentiated rejectors from non-rejectors immediately following renal ...
journal_title:Transplant immunology
pub_type: 杂志文章
doi:10.1016/j.trim.2005.02.003
更新日期:2005-06-01 00:00:00
abstract::Complex polygenic and multifactorial diseases remain a challenge for human geneticists. Here we aim to remind basic definitions of multifactorial diseases and the genetic related concepts underlying classical methods. Knowledge on pathophysiological process and the genetic information available conditions the design o...
journal_title:Transplant immunology
pub_type: 杂志文章,评审
doi:10.1016/j.trim.2005.03.015
更新日期:2005-08-01 00:00:00
abstract::The effect of blocking the expression of the mucosal addressin cell adhesion molecule-1 (MAdCAM-1) in a graft by an antibody, and immunohistochemical changes in the graft were monitored, using a rat small intestinal transplantation model. Dark Agouti (DA) rat small intestines were heterotopically transplanted into Lew...
journal_title:Transplant immunology
pub_type: 杂志文章
doi:10.1016/j.trim.2007.01.011
更新日期:2007-06-01 00:00:00
abstract:BACKGROUND:The potential of mesenchymal stem cells (MSCs) for immunosuppression has been tested in transplantation, but its mechanisms are not fully understood. This study investigated the role of MSC-expressing B7-H1 in the induction of immune tolerance to cardiac allografts by the combination therapy of MSCs and rapa...
journal_title:Transplant immunology
pub_type: 杂志文章
doi:10.1016/j.trim.2014.06.005
更新日期:2014-08-01 00:00:00