当前位置： SCI文献检索 > BMC BIOLOGY期刊下所有文献 > Cell type-specific expression profiling unravels the development and evolution of stinging cells in sea anemone.

Cell type-specific expression profiling unravels the development and evolution of stinging cells in sea anemone.

Abstract：

BACKGROUND:Cnidocytes are specialized cells that define the phylum Cnidaria. They possess an "explosive" organelle called cnidocyst that is important for prey capture and anti-predator defense. An extraordinary morphological and functional complexity of the cnidocysts has inspired numerous studies to investigate their structure and development. However, the transcriptomes of the cells bearing these unique organelles are yet to be characterized, impeding our understanding of the genetic basis of their biogenesis. RESULTS:In this study, we generated a nematocyte reporter transgenic line of the sea anemone Nematostella vectensis using the CRISPR/Cas9 system. By using a fluorescence-activated cell sorter (FACS), we have characterized cell type-specific transcriptomic profiles of various stages of cnidocyte maturation and showed that nematogenesis (the formation of functional cnidocysts) is underpinned by dramatic shifts in the spatiotemporal gene expression. Among the genes identified as upregulated in cnidocytes were Cnido-Jun and Cnido-Fos1-cnidarian-specific paralogs of the highly conserved c-Jun and c-Fos proteins of the stress-induced AP-1 transcriptional complex. The knockdown of the cnidocyte-specific c-Jun homolog by microinjection of morpholino antisense oligomer results in disruption of normal nematogenesis. CONCLUSIONS:Here, we show that the majority of upregulated genes and enriched biochemical pathways specific to cnidocytes are uncharacterized, emphasizing the need for further functional research on nematogenesis. The recruitment of the metazoan stress-related transcription factor c-Fos/c-Jun complex into nematogenesis highlights the evolutionary ingenuity and novelty associated with the formation of these highly complex, enigmatic, and phyletically unique organelles. Thus, we provide novel insights into the biology, development, and evolution of cnidocytes.

journal_name

BMC Biol

journal_title

BMC biology

authors

Sunagar K,Columbus-Shenkar YY,Fridrich A,Gutkovich N,Aharoni R,Moran Y

doi

10.1186/s12915-018-0578-4

subject

Has Abstract

pub_date

2018-09-27 00:00:00

pages

108

issue

1

issn

1741-7007

pii

10.1186/s12915-018-0578-4

journal_volume

16

pub_type

杂志文章

相关文献

BMC BIOLOGY文献大全
  • Synaptopathy, circuitopathy and the computational biology of Huntington's disease.

    abstract：:Huntington's disease (HD) is one of the most common tandem repeat disorders and presents as a unique trilogy of cognitive, psychiatric and motor symptoms. One of the major mysteries of HD is why it selectively affects specific neuronal populations. A new article in BMC Biology provides a piece in the puzzle of pathoge...

    journal_title：BMC biology

    pub_type： 评论,杂志文章

    doi：10.1186/s12915-018-0539-y

    authors： Hannan AJ

    更新日期：2018-06-27 00:00:00

  • Proteasome inhibition, the pursuit of new cancer therapeutics, and the adaptor molecule p130Cas.

    abstract：:Current interest in proteasome inhibitors for cancer therapy has stimulated considerable research efforts to identify the molecular pathway to their cytotoxicity with a view to identifying the mechanisms of sensitivity and resistance as well as informing the development of new drugs. Zhao and Vuori describe this month...

    journal_title：BMC biology

    pub_type： 评论,杂志文章

    doi：10.1186/1741-7007-9-72

    authors： Chauhan D,Anderson KC

    更新日期：2011-10-28 00:00:00

  • Nitrate enhances skeletal muscle fatty acid oxidation via a nitric oxide-cGMP-PPAR-mediated mechanism.

    abstract：BACKGROUND:Insulin sensitivity in skeletal muscle is associated with metabolic flexibility, including a high capacity to increase fatty acid (FA) oxidation in response to increased lipid supply. Lipid overload, however, can result in incomplete FA oxidation and accumulation of potentially harmful intermediates where mi...

    journal_title：BMC biology

    pub_type： 杂志文章

    doi：10.1186/s12915-015-0221-6

    authors： Ashmore T,Roberts LD,Morash AJ,Kotwica AO,Finnerty J,West JA,Murfitt SA,Fernandez BO,Branco C,Cowburn AS,Clarke K,Johnson RS,Feelisch M,Griffin JL,Murray AJ

    更新日期：2015-12-22 00:00:00

  • S28 peptidases: lessons from a seemingly 'dysfunctional' family of two.

    abstract：:A recent paper in BMC Structural Biology reports the crystal structure of human prolylcarboxypeptidase (PRCP), one of the two members of the S28 peptidase family. Comparison of the substrate-binding site of PRCP with that of its family partner, dipeptidyl dipeptidase 7 (DPP7), helps to explain the different enzymatic ...

    journal_title：BMC biology

    pub_type： 杂志文章

    doi：10.1186/1741-7007-8-87

    authors： Kozarich JW

    更新日期：2010-06-28 00:00:00

  • The intestinal microbiota regulates host cholesterol homeostasis.

    abstract：BACKGROUND:Management of blood cholesterol is a major focus of efforts to prevent cardiovascular diseases. The objective of this study was to investigate how the gut microbiota affects host cholesterol homeostasis at the organism scale. RESULTS:We depleted the intestinal microbiota of hypercholesterolemic female Apoe-...

    journal_title：BMC biology

    pub_type： 杂志文章

    doi：10.1186/s12915-019-0715-8

    authors： Le Roy T,Lécuyer E,Chassaing B,Rhimi M,Lhomme M,Boudebbouze S,Ichou F,Haro Barceló J,Huby T,Guerin M,Giral P,Maguin E,Kapel N,Gérard P,Clément K,Lesnik P

    更新日期：2019-11-27 00:00:00

  • Dorsal spine evolution in threespine sticklebacks via a splicing change in MSX2A.

    abstract：BACKGROUND:Dorsal spine reduction in threespine sticklebacks (Gasterosteus aculeatus) is a classic example of recurrent skeletal evolution in nature. Sticklebacks in marine environments typically have long spines that form part of their skeletal armor. Many derived freshwater populations have evolved shorter spines. Ch...

    journal_title：BMC biology

    pub_type： 杂志文章

    doi：10.1186/s12915-017-0456-5

    authors： Howes TR,Summers BR,Kingsley DM

    更新日期：2017-12-07 00:00:00

  • Parallel evolution of highly conserved plastid genome architecture in red seaweeds and seed plants.

    abstract：BACKGROUND:The red algae (Rhodophyta) diverged from the green algae and plants (Viridiplantae) over one billion years ago within the kingdom Archaeplastida. These photosynthetic lineages provide an ideal model to study plastid genome reduction in deep time. To this end, we assembled a large dataset of the plastid genom...

    journal_title：BMC biology

    pub_type： 杂志文章

    doi：10.1186/s12915-016-0299-5

    authors： Lee J,Cho CH,Park SI,Choi JW,Song HS,West JA,Bhattacharya D,Yoon HS

    更新日期：2016-09-02 00:00:00

  • An elevated plus-maze in mixed reality for studying human anxiety-related behavior.

    abstract：BACKGROUND:A dearth of laboratory tests to study actual human approach-avoidance behavior has complicated translational research on anxiety. The elevated plus-maze (EPM) is the gold standard to assess approach-avoidance behavior in rodents. METHODS:Here, we translated the EPM to humans using mixed reality through a co...

    journal_title：BMC biology

    pub_type： 杂志文章,随机对照试验

    doi：10.1186/s12915-017-0463-6

    authors： Biedermann SV,Biedermann DG,Wenzlaff F,Kurjak T,Nouri S,Auer MK,Wiedemann K,Briken P,Haaker J,Lonsdorf TB,Fuss J

    更新日期：2017-12-21 00:00:00

  • An automated aquatic rack system for rearing marine invertebrates.

    abstract：BACKGROUND:One hundred years ago, marine organisms were the dominant systems for the study of developmental biology. The challenges in rearing these organisms outside of a marine setting ultimately contributed to a shift towards work on a smaller number of so-called model systems. Those animals are typically non-marine...

    journal_title：BMC biology

    pub_type： 杂志文章

    doi：10.1186/s12915-020-00772-w

    authors： Henry JQ,Lesoway MP,Perry KJ

    更新日期：2020-05-04 00:00:00

  • LoxP-FRT Trap (LOFT): a simple and flexible system for conventional and reversible gene targeting.

    abstract：BACKGROUND:Conditional gene knockout (cKO) mediated by the Cre/LoxP system is indispensable for exploring gene functions in mice. However, a major limitation of this method is that gene KO is not reversible. A number of methods have been developed to overcome this, but each method has its own limitations. RESULTS:We d...

    journal_title：BMC biology

    pub_type： 杂志文章

    doi：10.1186/1741-7007-10-96

    authors： Chaiyachati BH,Kaundal RK,Zhao J,Wu J,Flavell R,Chi T

    更新日期：2012-11-30 00:00:00

  • Targeting protein quality control pathways in breast cancer.

    abstract：:The efficient production, folding, and secretion of proteins is critical for cancer cell survival. However, cancer cells thrive under stress conditions that damage proteins, so many cancer cells overexpress molecular chaperones that facilitate protein folding and target misfolded proteins for degradation via the ubiqu...

    journal_title：BMC biology

    pub_type： 杂志文章,评审

    doi：10.1186/s12915-017-0449-4

    authors： Sannino S,Brodsky JL

    更新日期：2017-11-16 00:00:00

  • Be the change you seek in science.

    abstract：:Few would argue that science is better done in silos, with no transparency or sharing of methods and resources. Yet scientists and scientific stakeholders (e.g., academic institutions, funding agencies, journals) alike continue to find themselves at a relative impasse in the implementation of open science practices, s...

    journal_title：BMC biology

    pub_type： 杂志文章

    doi：10.1186/s12915-019-0647-3

    authors： Milham MP,Klein A

    更新日期：2019-03-27 00:00:00

  • Precise A•T to G•C base editing in the zebrafish genome.

    abstract：BACKGROUND:Base editors are a class of genome editing tools with the ability to efficiently induce point mutations in genomic DNA, without inducing double-strand breaks or relying on homology-direct repair as in other such technologies. Recently, adenine base editors (ABEs) have been developed to mediate the conversion...

    journal_title：BMC biology

    pub_type： 杂志文章

    doi：10.1186/s12915-018-0609-1

    authors： Qin W,Lu X,Liu Y,Bai H,Li S,Lin S

    更新日期：2018-11-20 00:00:00

  • De novo assembly of the complex genome of Nippostrongylus brasiliensis using MinION long reads.

    abstract：BACKGROUND:Eukaryotic genome assembly remains a challenge in part due to the prevalence of complex DNA repeats. This is a particularly acute problem for holocentric nematodes because of the large number of satellite DNA sequences found throughout their genomes. These have been recalcitrant to most genome sequencing met...

    journal_title：BMC biology

    pub_type： 杂志文章

    doi：10.1186/s12915-017-0473-4

    authors： Eccles D,Chandler J,Camberis M,Henrissat B,Koren S,Le Gros G,Ewbank JJ

    更新日期：2018-01-11 00:00:00

  • Open questions: CRISPR biology.

    abstract：:CRISPR-Cas systems, the purveyors of adaptive immunity in archaea and bacteria and sources of the new generation of genome engineering tools, have been studied in exquisite molecular detail. However, when it comes to biological functions, ecology, and evolution of CRISPR-Cas, many more intriguing questions remain than...

    journal_title：BMC biology

    pub_type： 杂志文章

    doi：10.1186/s12915-018-0565-9

    authors： Koonin EV

    更新日期：2018-09-24 00:00:00

  • Organization and regulation of intracellular plasma membrane-connected HIV-1 assembly compartments in macrophages.

    abstract：BACKGROUND:In HIV-1-infected human monocyte-derived macrophages (MDMs), virus particles assemble primarily on intracellularly sequestered plasma membrane domains termed intracellular plasma membrane-connected compartments (IPMCs). Despite their clear role in virus formation, little is known of the organization, composi...

    journal_title：BMC biology

    pub_type： 杂志文章

    doi：10.1186/1741-7007-11-89

    authors： Mlcochova P,Pelchen-Matthews A,Marsh M

    更新日期：2013-08-02 00:00:00

  • Ankyrin repeat domain-encoding genes in the wPip strain of Wolbachia from the Culex pipiens group.

    abstract：BACKGROUND:Wolbachia are obligate endosymbiotic bacteria maternally transmitted through the egg cytoplasm that are responsible for several reproductive disorders in their insect hosts, such as cytoplasmic incompatibility (CI) in infected mosquitoes. Species in the Culex pipiens complex display an unusually high number ...

    journal_title：BMC biology

    pub_type： 杂志文章

    doi：10.1186/1741-7007-5-39

    authors： Walker T,Klasson L,Sebaihia M,Sanders MJ,Thomson NR,Parkhill J,Sinkins SP

    更新日期：2007-09-20 00:00:00

  • Will human influences on evolutionary dynamics in the wild pervade the Anthropocene?

    abstract：:The five most pervasive anthropogenic threats to biodiversity are over-exploitation, habitat changes, climate change, invasive species, and pollution. Since all of these threats can affect intraspecific biodiversity-including genetic variation within populations-humans have the potential to induce contemporary microev...

    journal_title：BMC biology

    pub_type： 杂志文章,评审

    doi：10.1186/s12915-017-0476-1

    authors： Pelletier F,Coltman DW

    更新日期：2018-01-15 00:00:00

  • Spontaneous focal activation of invariant natural killer T (iNKT) cells in mouse liver and kidney.

    abstract：BACKGROUND:Invariant natural killer T (iNKT) cells differ from other T cells by their hyperactive effector T-cell status, in addition to the expression of NK lineage receptors and semi-invariant T-cell receptors. It is generally agreed that the immune phenotype of iNKT cells is maintained by repeated activation in peri...

    journal_title：BMC biology

    pub_type： 杂志文章

    doi：10.1186/1741-7007-8-142

    authors： Zeng J,Howard JC

    更新日期：2010-11-30 00:00:00

  • A spruce gene map infers ancient plant genome reshuffling and subsequent slow evolution in the gymnosperm lineage leading to extant conifers.

    abstract：BACKGROUND:Seed plants are composed of angiosperms and gymnosperms, which diverged from each other around 300 million years ago. While much light has been shed on the mechanisms and rate of genome evolution in flowering plants, such knowledge remains conspicuously meagre for the gymnosperms. Conifers are key representa...

    journal_title：BMC biology

    pub_type： 杂志文章

    doi：10.1186/1741-7007-10-84

    authors： Pavy N,Pelgas B,Laroche J,Rigault P,Isabel N,Bousquet J

    更新日期：2012-10-26 00:00:00

  • Replicates and repeats.

    abstract：:The authors of this paper were interested to see whether the expression of three proteins (A, B and C) was altered in a knockout mouse model of a gene encoding Protein X. Each experiment measured the expression of a different protein and the results seem to show a clear increase in protein expression in the mutant mou...

    journal_title：BMC biology

    pub_type： 杂志文章

    doi：10.1186/s12915-016-0254-5

    authors： Bell G

    更新日期：2016-04-07 00:00:00

  • Reagent and laboratory contamination can critically impact sequence-based microbiome analyses.

    abstract：BACKGROUND:The study of microbial communities has been revolutionised in recent years by the widespread adoption of culture independent analytical techniques such as 16S rRNA gene sequencing and metagenomics. One potential confounder of these sequence-based approaches is the presence of contamination in DNA extraction ...

    journal_title：BMC biology

    pub_type： 杂志文章

    doi：10.1186/s12915-014-0087-z

    authors： Salter SJ,Cox MJ,Turek EM,Calus ST,Cookson WO,Moffatt MF,Turner P,Parkhill J,Loman NJ,Walker AW

    更新日期：2014-11-12 00:00:00

  • A fast recoiling silk-like elastomer facilitates nanosecond nematocyst discharge.

    abstract：BACKGROUND:The discharge of the Cnidarian stinging organelle, the nematocyst, is one of the fastest processes in biology and involves volume changes of the highly pressurised (150 bar) capsule of up to 50%. Hitherto, the molecular basis for the unusual biomechanical properties of nematocysts has been elusive, as their ...

    journal_title：BMC biology

    pub_type： 杂志文章

    doi：10.1186/s12915-014-0113-1

    authors： Beckmann A,Xiao S,Müller JP,Mercadante D,Nüchter T,Kröger N,Langhojer F,Petrich W,Holstein TW,Benoit M,Gräter F,Özbek S

    更新日期：2015-01-16 00:00:00

  • Features of the ancestral bilaterian inferred from Platynereis dumerilii ParaHox genes.

    abstract：BACKGROUND:The ParaHox gene cluster is the evolutionary sister to the Hox cluster. Whilst the role of the Hox cluster in patterning the anterior-posterior axis of bilaterian animals is well established, and the organisation of vertebrate Hox clusters is intimately linked to gene regulation, much less is known about the...

    journal_title：BMC biology

    pub_type： 杂志文章

    doi：10.1186/1741-7007-7-43

    authors： Hui JH,Raible F,Korchagina N,Dray N,Samain S,Magdelenat G,Jubin C,Segurens B,Balavoine G,Arendt D,Ferrier DE

    更新日期：2009-07-23 00:00:00

  • Downregulation of the NLRP3 inflammasome by adiponectin rescues Duchenne muscular dystrophy.

    abstract：BACKGROUND:The hormone adiponectin (ApN) exerts powerful anti-inflammatory effects on skeletal muscle and can reverse devastating myopathies, like Duchenne muscular dystrophy (DMD), where inflammation exacerbates disease progression. The NLRP3 inflammasome plays a key role in the inflammation process, and its aberrant ...

    journal_title：BMC biology

    pub_type： 杂志文章

    doi：10.1186/s12915-018-0501-z

    authors： Boursereau R,Abou-Samra M,Lecompte S,Noel L,Brichard SM

    更新日期：2018-03-20 00:00:00

  • A metabolic signature of long life in Caenorhabditis elegans.

    abstract：BACKGROUND:Many Caenorhabditis elegans mutations increase longevity and much evidence suggests that they do so at least partly via changes in metabolism. However, up until now there has been no systematic investigation of how the metabolic networks of long-lived mutants differ from those of normal worms. Metabolomic te...

    journal_title：BMC biology

    pub_type： 杂志文章

    doi：10.1186/1741-7007-8-14

    authors： Fuchs S,Bundy JG,Davies SK,Viney JM,Swire JS,Leroi AM

    更新日期：2010-02-10 00:00:00

  • LIM homeodomain transcription factor Isl1 directs normal pyloric development by targeting Gata3.

    abstract：BACKGROUND:Abnormalities in pyloric development or in contractile function of the pylorus cause reflux of duodenal contents into the stomach and increase the risk of gastric metaplasia and cancer. Abnormalities of the pyloric region are also linked to congenital defects such as the relatively common neonatal hypertroph...

    journal_title：BMC biology

    pub_type： 杂志文章

    doi：10.1186/1741-7007-12-25

    authors： Li Y,Pan J,Wei C,Chen J,Liu Y,Liu J,Zhang X,Evans SM,Cui Y,Cui S

    更新日期：2014-03-27 00:00:00

  • The hidden diversity of ribosomal peptide natural products.

    abstract：:A recent report in BMC Biology on the discovery and analysis of biosynthetic genes for ribosomal peptide natural products confirms that these pathways are much more common and diverse than previously suspected, contributing substantially to the chemical arsenal employed by bacteria. See research article http://www.bio...

    journal_title：BMC biology

    pub_type： 评论,杂志文章

    doi：10.1186/1741-7007-8-83

    authors： Schmidt EW

    更新日期：2010-06-17 00:00:00

  • The study of Priapulus caudatus reveals conserved molecular patterning underlying different gut morphogenesis in the Ecdysozoa.

    abstract：BACKGROUND:The digestive systems of animals can become highly specialized in response to their exploration and occupation of new ecological niches. Although studies on different animals have revealed commonalities in gut formation, the model systems Caenorhabditis elegans and Drosophila melanogaster, which belong to th...

    journal_title：BMC biology

    pub_type： 杂志文章

    doi：10.1186/s12915-015-0139-z

    authors： Martín-Durán JM,Hejnol A

    更新日期：2015-04-21 00:00:00

  • Erv1 of Arabidopsis thaliana can directly oxidize mitochondrial intermembrane space proteins in the absence of redox-active Mia40.

    abstract：BACKGROUND:Many proteins of the mitochondrial intermembrane space (IMS) contain structural disulfide bonds formed by the mitochondrial disulfide relay. In fungi and animals, the sulfhydryl oxidase Erv1 'generates' disulfide bonds that are passed on to the oxidoreductase Mia40, which oxidizes substrate proteins. A diffe...

    journal_title：BMC biology

    pub_type： 杂志文章

    doi：10.1186/s12915-017-0445-8

    authors： Peleh V,Zannini F,Backes S,Rouhier N,Herrmann JM

    更新日期：2017-11-08 00:00:00