Abstract:
BACKGROUND:Abnormalities in pyloric development or in contractile function of the pylorus cause reflux of duodenal contents into the stomach and increase the risk of gastric metaplasia and cancer. Abnormalities of the pyloric region are also linked to congenital defects such as the relatively common neonatal hypertrophic pyloric stenosis, and primary duodenogastric reflux. Therefore, understanding pyloric development is of great clinical relevance. Here, we investigated the role of the LIM homeodomain transcription factor Isl1 in pyloric development. RESULTS:Examination of Isl1 expression in developing mouse stomach by immunohistochemistry, whole mount in situ hybridization and real-time quantitative PCR demonstrated that Isl1 is highly expressed in developing mouse stomach, principally in the smooth muscle layer of the pylorus. Isl1 expression was also examined by immunofluorescence in human hypertrophic pyloric stenosis where the majority of smooth muscle cells were found to express Isl1. Isl1 function in embryonic stomach development was investigated utilizing a tamoxifen-inducible Isl1 knockout mouse model. Isl1 deficiency led to nearly complete absence of the pyloric outer longitudinal muscle layer at embryonic day 18.5, which is consistent with Gata3 null mouse phenotype. Chromatin immunoprecipitation, luciferase assays, and electrophoretic mobility shift assays revealed that Isl1 ensures normal pyloric development by directly targeting Gata3. CONCLUSIONS:This study demonstrates that the Isl1-Gata3 transcription regulatory axis is essential for normal pyloric development. These findings are highly clinically relevant and may help to better understand pathways leading to pyloric disease.
journal_name
BMC Bioljournal_title
BMC biologyauthors
Li Y,Pan J,Wei C,Chen J,Liu Y,Liu J,Zhang X,Evans SM,Cui Y,Cui Sdoi
10.1186/1741-7007-12-25subject
Has Abstractpub_date
2014-03-27 00:00:00pages
25issn
1741-7007pii
1741-7007-12-25journal_volume
12pub_type
杂志文章相关文献
BMC BIOLOGY文献大全abstract::CRISPR-Cas systems, the purveyors of adaptive immunity in archaea and bacteria and sources of the new generation of genome engineering tools, have been studied in exquisite molecular detail. However, when it comes to biological functions, ecology, and evolution of CRISPR-Cas, many more intriguing questions remain than...
journal_title:BMC biology
pub_type: 杂志文章
doi:10.1186/s12915-018-0565-9
更新日期:2018-09-24 00:00:00
abstract:BACKGROUND:Nucleolar rDNA is tightly associated with silent heterochromatin, which is important for rDNA stability, nucleolar integration, and cellular senescence. Two pathways have been described that lead to rDNA silencing in yeast: 1) the RENT (regulator of nucleolar silencing and telophase exit) complex, which is c...
journal_title:BMC biology
pub_type: 杂志文章
doi:10.1186/s12915-014-0075-3
更新日期:2014-09-24 00:00:00
abstract:BACKGROUND:Ionotropic receptors (IRs) are a large, divergent subfamily of ionotropic glutamate receptors (iGluRs) that are expressed in diverse peripheral sensory neurons and function in olfaction, taste, hygrosensation and thermosensation. Analogous to the cell biological properties of their synaptic iGluR ancestors, ...
journal_title:BMC biology
pub_type: 杂志文章
doi:10.1186/s12915-019-0651-7
更新日期:2019-04-17 00:00:00
abstract:BACKGROUND:Dorsal spine reduction in threespine sticklebacks (Gasterosteus aculeatus) is a classic example of recurrent skeletal evolution in nature. Sticklebacks in marine environments typically have long spines that form part of their skeletal armor. Many derived freshwater populations have evolved shorter spines. Ch...
journal_title:BMC biology
pub_type: 杂志文章
doi:10.1186/s12915-017-0456-5
更新日期:2017-12-07 00:00:00
abstract:BACKGROUND:Dinoflagellates are taxonomically diverse and ecologically important phytoplankton that are ubiquitously present in marine and freshwater environments. Mostly photosynthetic, dinoflagellates provide the basis of aquatic primary production; most taxa are free-living, while some can form symbiotic and parasiti...
journal_title:BMC biology
pub_type: 杂志文章
doi:10.1186/s12915-020-00782-8
更新日期:2020-05-24 00:00:00
abstract:BACKGROUND:Loss of photosynthesis has occurred independently in several plant and algal lineages, and represents a major metabolic shift with potential consequences for the content and structure of plastid genomes. To investigate such changes, we sequenced the complete plastid genome of the parasitic, non-photosyntheti...
journal_title:BMC biology
pub_type: 杂志文章
doi:10.1186/1741-7007-4-12
更新日期:2006-04-21 00:00:00
abstract:BACKGROUND:One hundred years ago, marine organisms were the dominant systems for the study of developmental biology. The challenges in rearing these organisms outside of a marine setting ultimately contributed to a shift towards work on a smaller number of so-called model systems. Those animals are typically non-marine...
journal_title:BMC biology
pub_type: 杂志文章
doi:10.1186/s12915-020-00772-w
更新日期:2020-05-04 00:00:00
abstract:BACKGROUND:The digestive systems of animals can become highly specialized in response to their exploration and occupation of new ecological niches. Although studies on different animals have revealed commonalities in gut formation, the model systems Caenorhabditis elegans and Drosophila melanogaster, which belong to th...
journal_title:BMC biology
pub_type: 杂志文章
doi:10.1186/s12915-015-0139-z
更新日期:2015-04-21 00:00:00
abstract:BACKGROUND:Invariant natural killer T (iNKT) cells differ from other T cells by their hyperactive effector T-cell status, in addition to the expression of NK lineage receptors and semi-invariant T-cell receptors. It is generally agreed that the immune phenotype of iNKT cells is maintained by repeated activation in peri...
journal_title:BMC biology
pub_type: 杂志文章
doi:10.1186/1741-7007-8-142
更新日期:2010-11-30 00:00:00
abstract:BACKGROUND:In HIV-1-infected human monocyte-derived macrophages (MDMs), virus particles assemble primarily on intracellularly sequestered plasma membrane domains termed intracellular plasma membrane-connected compartments (IPMCs). Despite their clear role in virus formation, little is known of the organization, composi...
journal_title:BMC biology
pub_type: 杂志文章
doi:10.1186/1741-7007-11-89
更新日期:2013-08-02 00:00:00
abstract:BACKGROUND:The study of microbial communities has been revolutionised in recent years by the widespread adoption of culture independent analytical techniques such as 16S rRNA gene sequencing and metagenomics. One potential confounder of these sequence-based approaches is the presence of contamination in DNA extraction ...
journal_title:BMC biology
pub_type: 杂志文章
doi:10.1186/s12915-014-0087-z
更新日期:2014-11-12 00:00:00
abstract:BACKGROUND:Methods for assigning strains to bacterial species are cumbersome and no longer fit for purpose. The concatenated sequences of multiple house-keeping genes have been shown to be able to define and circumscribe bacterial species as sequence clusters. The advantage of this approach (multilocus sequence analysi...
journal_title:BMC biology
pub_type: 杂志文章
doi:10.1186/1741-7007-7-3
更新日期:2009-01-26 00:00:00
abstract:BACKGROUND:Regulatory T cells (Tregs) expressing the transcription factor FOXP3 are crucial mediators of self-tolerance, preventing autoimmune diseases but possibly hampering tumor rejection. Clinical manipulation of Tregs is of great interest, and first-in-man trials of Treg transfer have achieved promising outcomes. ...
journal_title:BMC biology
pub_type: 杂志文章
doi:10.1186/s12915-018-0518-3
更新日期:2018-05-07 00:00:00
abstract::The expansion of genome sequencing projects has produced accumulating evidence for lateral transfer of genes between prokaryotic and eukaryotic genomes. However, it remains controversial whether these genes are of functional importance in their recipient host. Nikoh and Nakabachi, in a recent paper in BMC Biology, tak...
journal_title:BMC biology
pub_type: 评论,杂志文章
doi:10.1186/1741-7007-7-20
更新日期:2009-05-05 00:00:00
abstract:BACKGROUND:Affinity purification followed by mass spectrometry (AP/MS) is a widely used approach to identify protein interactions and complexes. In multicellular organisms, the accurate identification of protein complexes by AP/MS is complicated by the potential heterogeneity of complexes in different tissues. Here, we...
journal_title:BMC biology
pub_type: 杂志文章
doi:10.1186/s12915-016-0286-x
更新日期:2016-08-09 00:00:00
abstract:BACKGROUND:Base editors are a class of genome editing tools with the ability to efficiently induce point mutations in genomic DNA, without inducing double-strand breaks or relying on homology-direct repair as in other such technologies. Recently, adenine base editors (ABEs) have been developed to mediate the conversion...
journal_title:BMC biology
pub_type: 杂志文章
doi:10.1186/s12915-018-0609-1
更新日期:2018-11-20 00:00:00
abstract:BACKGROUND:A key event in plant morphogenesis is the establishment of a division plane. A plant-specific microtubular preprophase band (PPB) accurately predicts the line of cell division, whereas the phragmoplast, another plant-specific array, executes cell division by maintaining this predicted line. Although establis...
journal_title:BMC biology
pub_type: 杂志文章
doi:10.1186/1741-7007-3-11
更新日期:2005-04-14 00:00:00
abstract:BACKGROUND:Accurate determination of mouse positions from video data is crucial for various types of behavioral analyses. While detection of body positions is straightforward, the correct identification of nose positions, usually more informative, is far more challenging. The difficulty is largely due to variability in...
journal_title:BMC biology
pub_type: 杂志文章
doi:10.1186/s12915-017-0377-3
更新日期:2017-05-15 00:00:00
abstract:BACKGROUND:Internal tagging of proteins by inserting small functional peptides into surface accessible permissive sites has proven to be an indispensable tool for basic and applied science. Permissive sites are typically identified by transposon mutagenesis on a case-by-case basis, limiting scalability and their exploi...
journal_title:BMC biology
pub_type: 杂志文章
doi:10.1186/s12915-017-0440-0
更新日期:2017-10-30 00:00:00
abstract:BACKGROUND:The pituitary gland is formed by the juxtaposition of two tissues: neuroectoderm arising from the basal diencephalon, and oral epithelium, which invaginates towards the central nervous system from the roof of the mouth. The oral invagination that reaches the brain from the mouth is referred to as Rathke's po...
journal_title:BMC biology
pub_type: 杂志文章
doi:10.1186/1741-7007-11-27
更新日期:2013-03-28 00:00:00
abstract::Malaria is a common and life-threatening disease endemic in large parts of the world. The emergence of antimalarial drug resistance is threatening disease-control measures that depend heavily on treatment of clinical malaria. The intracellular malaria parasite is particularly vulnerable during its brief extracellular ...
journal_title:BMC biology
pub_type: 评论,杂志文章
doi:10.1186/s12915-015-0185-6
更新日期:2015-09-08 00:00:00
abstract:BACKGROUND:O-fucosyltransferase1 (OFUT1) is a conserved ER protein essential for Notch signaling. OFUT1 glycosylates EGF domains, which can then be further modified by the N-acetylglucosaminyltransferase Fringe. OFUT1 also possesses a chaperone activity that promotes the folding and secretion of Notch. Here, we investi...
journal_title:BMC biology
pub_type: 杂志文章
doi:10.1186/1741-7007-6-1
更新日期:2008-01-14 00:00:00
abstract:BACKGROUND:Rickettsia are intracellular symbionts of eukaryotes that are best known for infecting and causing serious diseases in humans and other mammals. All known vertebrate-associated Rickettsia are vectored by arthropods as part of their life-cycle, and many other Rickettsia are found exclusively in arthropods wit...
journal_title:BMC biology
pub_type: 杂志文章
doi:10.1186/1741-7007-7-6
更新日期:2009-02-02 00:00:00
abstract::Mitochondria are highly dynamic and structurally complex organelles that provide multiple essential metabolic functions. Mitochondrial dysfunction is associated with neurodegenerative conditions such as Parkinson's disease, as well as bacterial infection. Here, we explore the roles of mitochondrial autophagy (mitophag...
journal_title:BMC biology
pub_type: 杂志文章,评审
doi:10.1186/s12915-015-0129-1
更新日期:2015-04-03 00:00:00
abstract::The mitogen-activated protein kinase p38 (p38 MAPK) is activated by a number of stresses. A recent study in BMC Genomics has uncovered the early transcriptional responses to three types of stress and has demonstrated a central role for p38 MAPK in mediating these responses. See research article http://www.biomedcentra...
journal_title:BMC biology
pub_type: 评论,社论
doi:10.1186/1741-7007-8-47
更新日期:2010-04-27 00:00:00
abstract:BACKGROUND:Archaeological studies have revealed a series of cultural changes around the Last Glacial Maximum in East Asia; whether these changes left any signatures in the gene pool of East Asians remains poorly indicated. To achieve deeper insights into the demographic history of modern humans in East Asia around the ...
journal_title:BMC biology
pub_type: 杂志文章
doi:10.1186/1741-7007-9-2
更新日期:2011-01-10 00:00:00
abstract:BACKGROUND:Metazoan lineages exhibit a wide range of regenerative capabilities that vary among developmental stage and tissue type. The most robust regenerative abilities are apparent in the phyla Cnidaria, Platyhelminthes, and Echinodermata, whose members are capable of whole-body regeneration (WBR). This phenomenon h...
journal_title:BMC biology
pub_type: 杂志文章
doi:10.1186/s12915-019-0633-9
更新日期:2019-02-22 00:00:00
abstract::Few would argue that science is better done in silos, with no transparency or sharing of methods and resources. Yet scientists and scientific stakeholders (e.g., academic institutions, funding agencies, journals) alike continue to find themselves at a relative impasse in the implementation of open science practices, s...
journal_title:BMC biology
pub_type: 杂志文章
doi:10.1186/s12915-019-0647-3
更新日期:2019-03-27 00:00:00
abstract:BACKGROUND:Cellular functions can be regulated by cell-cell interactions that are influenced by extra-cellular, density-dependent signaling factors. Dictyostelium grow as individual cells in nutrient-rich sources, but, as nutrients become depleted, they initiate a multi-cell developmental program that is dependent upon...
journal_title:BMC biology
pub_type: 杂志文章
doi:10.1186/s12915-019-0714-9
更新日期:2019-12-02 00:00:00
abstract::The evolution of plastids from cyanobacteria is believed to represent a singularity in the history of life. The enigmatic amoeba Paulinella and its 'recently' acquired photosynthetic inclusions provide a fascinating system through which to gain fresh insight into how endosymbionts become organelles.The plastids, or ch...
journal_title:BMC biology
pub_type: 杂志文章
doi:10.1186/1741-7007-10-35
更新日期:2012-04-24 00:00:00