Abstract:
Background & Objectives:Molecular genetic abnormalities have a significant role not only in diagnosis but also in determining the clinical course and prognosis. Nucleophosmin-1 (NPM-1) is associated with good prognosis while internal tandem duplication of the fms-like tyrosine kinase-3 gene (FLT3-ITD) confers a poor prognosis. Knowledge of the status of these mutations in AML patients not only guides treatment decisions but also helps in predicting response to frontline induction and consolidation chemotherapy as well as the risk of relapse and overall survival. Our objectives were to determine the prevalence, clinico-haematological features and immunophenotypic characteristics of AML patients with FLT3-ITD and NPM1 mutation and to evaluate the response to induction therapy (CR) and disease free survival (DFS) in this cohort of patients. Methods:Patients diagnosed as AML from March 2015 to March 2017 at Armed Forces Institute of Pathology Rawalpindi were included in the study. Clinico-haematologic and immunophenotypic parameters were noted and molecular analysis for FLT3-ITD and NPM1 mutation was performed. Any correlation with cytogenetics or other molecular markers was also studied. Response to standard induction chemotherapy and disease-free survival were assessed. Results:A total of 108 cases of AML were analyzed. Median age was 35 years and 64.8% were males. The median age of the study group was 35 years. Of these, 70 (64.8%) were males while 38 (35.2%) were females. Twenty-nine (26.9%) patients were NPM1 positive, twelve (11.1%) were FLT3-ITD positive while eight (7.4%) were positive for both mutations. Patients with NPM1 mutations were associated with female gender, higher haemoglobin level and platelet counts while those with FLT3-ITD mutations were predominantly seen in male patients and had significantly higher WBC counts, bone marrow blasts, biopsy cellularity and LDH levels. CR rates of NPM1 positive, FLT3-ITD positive and both mutation positive groups were 72%, 60% and 71%, respectively. The median disease-free survival was significantly lower in the FLT3-ITD positive group (7.1 months) as compared to the NPM1 positive group (16.1 months). The median disease-free survival was 12 months and 11.9 months in the NPM1 positive/FLT3-ITD positive and the NPM1 negative/FLT3-ITD negative groups, respectively. Conclusion:AML patients harbouring NPM1 and FLT3-ITD mutations have distinct clinical and haematological characteristics. NPM1 mutations have a better CR and DFS as compared to FLT3-ITD group.
journal_name
Pak J Med Scijournal_title
Pakistan journal of medical sciencesauthors
Mahmood R,Altaf C,Malik HS,Khan SAdoi
10.12669/pjms.35.1.285subject
Has Abstractpub_date
2019-01-01 00:00:00pages
23-28issue
1eissn
1682-024Xissn
1681-715Xpii
PJMS-35-23journal_volume
35pub_type
杂志文章abstract:Objective:To identify the perceptions of the faculty about their current level of pedagogical skills and their desire to improve these skills in future. Methods:This cross-sectional study was conducted at two medical colleges of Dow University of Health Sciences, Karachi from March 1, 2015 to April 30, 2015. A re-vali...
journal_title:Pakistan journal of medical sciences
pub_type: 杂志文章
doi:10.12669/pjms.346.16302
更新日期:2018-11-01 00:00:00
abstract:OBJECTIVE:To determine insulin resistance in non-diabetic chronic hepatitis C patients using Homeostatic Model Assessment of Insulin Resistance (HOMA-IR). METHODOLOGY:Patients having anti-HCV positive were included in this study. Patients with diabetes mellitus, thyroid disease, hyperlipidemias, hypercortisolism and i...
journal_title:Pakistan journal of medical sciences
pub_type: 杂志文章
doi:10.12669/pjms.291.2888
更新日期:2013-01-01 00:00:00
abstract:OBJECTIVE:To assess inpatient management of non-critically ill type 2 diabetics with different insulin regimen. METHODS:We reviewed the medical records of all non-critically ill type 2 diabetic patients more than 18 years of age in medical department of civil hospital Karachi and Dow University of Health Sciences from...
journal_title:Pakistan journal of medical sciences
pub_type: 杂志文章
doi:
更新日期:2014-07-01 00:00:00
abstract:Objective:To compare the efficacy of oral Labetalol versus oral Nifedipine for the treatment of postpartum hypertension. Methods:A prospective randomized controlled trial with parallel assignment was conducted in the department of Obstetrics and Gynecology, Dow University of Health Sciences Karachi, Pakistan, 124 pati...
journal_title:Pakistan journal of medical sciences
pub_type: 杂志文章
doi:10.12669/pjms.35.5.812
更新日期:2019-09-01 00:00:00
abstract:Objectives:To assess maternal and fetal morbidity associated with placenta previa and morbidly adherent placenta (MAP). Methods:All patients with placenta previa who delivered in services hospital from April 1, 2017 to March 31, 2019 were included. Maternal and fetal outcomes were compared amongst patients with placen...
journal_title:Pakistan journal of medical sciences
pub_type: 杂志文章
doi:10.12669/pjms.36.5.1647
更新日期:2020-07-01 00:00:00
abstract:OBJECTIVE:To explore the correlation between magnetic resonance imaging (MRI) features of uterine fibroids (UFs) and therapeutic effects of high-intensity focused ultrasound ablation (HIFUA), and to provide evidence for UFs diagnosis with MRI in clinical practice. METHODS:Forty-three UFs patients who were treated in o...
journal_title:Pakistan journal of medical sciences
pub_type: 杂志文章
doi:10.12669/pjms.314.7294
更新日期:2015-07-01 00:00:00
abstract:OBJECTIVE:To assess the efficacy of intercostal nerve protection by intercostal muscle (ICM) flap in post-thoracotomy pain improvement compared to intracostal suturing. METHODS:In a randomized controlled trial, ninety-four patients undergoing posterolateral thoracotomy surgery were divided into two subgroups. Intracos...
journal_title:Pakistan journal of medical sciences
pub_type: 杂志文章
doi:10.12669/pjms.331.11581
更新日期:2017-01-01 00:00:00
abstract:Objective:Hepatitis is a serious health concern with a high rate of mortality and morbidity world over. Saudi Arabia also has its course of the disease incidence. The data on the prevalence of the disease is still limiting. This study aimed to estimate the prevalence of hepatitis B virus [HBV] and hepatitis C virus [HC...
journal_title:Pakistan journal of medical sciences
pub_type: 杂志文章
doi:10.12669/pjms.342.14278
更新日期:2018-03-01 00:00:00
abstract:Objective:To investigate the predictive validity of Uniform Entrance Test for academic performance in the first two years in various health science degree programs. Methods:A retrospective analysis of admissions data and academic performance of students admitted in under-graduate programs of medicine, dentistry and ph...
journal_title:Pakistan journal of medical sciences
pub_type: 杂志文章
doi:10.12669/pjms.35.2.334
更新日期:2019-03-01 00:00:00
abstract:OBJECTIVES:To evaluate Multiple Choice and Short Essay Question items in Basic Medical Sciences by determining item writing flaws (IWFs) of MCQs along with cognitive level of each item in both methods. METHODS:This analytical study evaluated the quality of the assessment tools used for the first batch in a newly estab...
journal_title:Pakistan journal of medical sciences
pub_type: 杂志文章
doi:10.12669/pjms.301.4458
更新日期:2014-01-01 00:00:00
abstract:Objective:To determine the efficacy of oral eplerenone in anatomical and functional improvement in patients with chronic central serous chorioretinopathy (CSCR). Methods:This quasi experimental study was performed at PNS Shifa Hospital Karachi from September 2018 to February 2019. Study included 23 patients. Patients ...
journal_title:Pakistan journal of medical sciences
pub_type: 杂志文章
doi:10.12669/pjms.35.6.896
更新日期:2019-11-01 00:00:00
abstract:Background and Objective:Cardiovascular deaths usually occur in older pregnancies and arrhythmia is the third most common cause. Our study aimed to determine whether the risk of arrhythmia increases in pregnancy with advanced age. Methods:In total, 280 pregnant women, of whom 98 were of advanced age and 182 were under...
journal_title:Pakistan journal of medical sciences
pub_type: 杂志文章
doi:10.12669/pjms.343.14313
更新日期:2018-05-01 00:00:00
abstract:Objective:Hypertrophic scar is common in burn patients, but treating result could not meet the expectation of the patients and doctors. We have found that certain concentration level of lipopolysaccharide (LPS) stimulated normal fibroblast cells have statistically similar with fibroblast cells from hypertrophic scar on...
journal_title:Pakistan journal of medical sciences
pub_type: 杂志文章
doi:10.12669/pjms.341.13636
更新日期:2018-01-01 00:00:00
abstract:Objective:To investigate the effect of vitamin D deficiency on the levels of thyroid stimulating hormone (TSH), thyroid peroxidase antibody (TPO-Ab), interleukin-1 (IL-1) and high-sensitivity C-reactive protein (hs-CRP) in pregnant women with early pregnancy complicated by subclinical hypothyroidism. Methods:A total o...
journal_title:Pakistan journal of medical sciences
pub_type: 杂志文章
doi:10.12669/pjms.36.6.1982
更新日期:2020-09-01 00:00:00
abstract:OBJECTIVE:Finite element analysis was used to compare preoperative and postoperative stress distribution of a bone healing model of femur fracture, to identify whether broken ends of fractured bone would break or not after fixation dislodgement one year after intramedullary nailing. Method s: Using fast, personalized i...
journal_title:Pakistan journal of medical sciences
pub_type: 杂志文章
doi:
更新日期:2014-03-01 00:00:00
abstract:Objective:A paradigm shift towards a PBL bidirectional dialogic feedback can enhance learners' performance. This study aimed to investigate undergraduate medical students' perceptions of their PBL feedback. Methods:We sent e-mail invitations to a web-based survey to year one and two students at College of Medicine, Ki...
journal_title:Pakistan journal of medical sciences
pub_type: 杂志文章
doi:10.12669/pjms.36.7.1778
更新日期:2020-11-01 00:00:00
abstract:Background and Objective:Dengue infection has evolved into an epidemic during last few years in Pakistan and has been associated with poor outcomes. Literature with respect to mortality risk factors in Dengue infection is not sufficient. This compelled us to conduct this study to find out major contributory factors to ...
journal_title:Pakistan journal of medical sciences
pub_type: 杂志文章
doi:10.12669/pjms.335.13051
更新日期:2017-09-01 00:00:00
abstract:OBJECTIVE:This is aimed at investigating some coagulation and haematologic profile of HIV positive patients on highly active antiretroviral therapy in patients attending clinic at the University of Benin Teaching Hospital. METHODS:This is a correlation study comprising fifty (50) HIV positive patients on HAART between...
journal_title:Pakistan journal of medical sciences
pub_type: 杂志文章
doi:10.12669/pjms.316.7943
更新日期:2015-11-01 00:00:00
abstract:Objectives:To measure correlation and concordance between measured LDL cholesterol (mLDLc) and Friedewald's calculated LDL cholesterol (cLDLc). To compare the mLDLc and cLDLc values for various anthropometric measures and biochemical indices including insulin resistance, nephropathy, glycated hemoglobin and triglycerid...
journal_title:Pakistan journal of medical sciences
pub_type: 杂志文章
doi:10.12669/pjms.35.2.679
更新日期:2019-03-01 00:00:00
abstract:BACKGROUND & OBJECTIVE:Rehabilitation focuses brain-behavior relationship which highlights interaction between psychological and neurobiological factors for better patient care. There is a missing link in the literature about socio-cognitive aspects of frontal lobe epilepsy. Our objective was to examine prefrontal cort...
journal_title:Pakistan journal of medical sciences
pub_type: 杂志文章
doi:10.12669/pjms.331.11742
更新日期:2017-01-01 00:00:00
abstract:Objective:Work-related asthma (WRA) is one of the most common occupational diseases. In this study, we aimed to review diagnosing procedures and the characteristics of patients who were diagnosed with WRA. Methods:Between November 2013 and June 2016; 214 patients were referred to our clinic with WRA suspicion by an oc...
journal_title:Pakistan journal of medical sciences
pub_type: 杂志文章
doi:10.12669/pjms.335.12923
更新日期:2017-09-01 00:00:00
abstract:OBJECTIVE:The present study aimed to assess the prevalence of decreased visual acuity, strabismus, and spectacle wear in children aged 5 to 13 years. METHODS:A cross-sectional study was performed in primary education schools. A total of 1938 participants, including 940 females (48.5%) and 998 males (51.5%) with a mean...
journal_title:Pakistan journal of medical sciences
pub_type: 杂志文章
doi:10.12669/pjms.325.10367
更新日期:2016-09-01 00:00:00
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journal_title:Pakistan journal of medical sciences
pub_type: 杂志文章
doi:10.12669/pjms.301.3706
更新日期:2014-01-01 00:00:00
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journal_title:Pakistan journal of medical sciences
pub_type: 杂志文章
doi:10.12669/pjms.333.12265
更新日期:2017-05-01 00:00:00
abstract:Objective:To compare the effectiveness of learning procedural skills on patients versus mannequins and models. Methods:Seventy four interns from two consecutive batches at the Department of Obstetrics and Gynaecolgy Unit-I at Jinnah Postgraduate Medical Center Karachi participated in the study between April and Septem...
journal_title:Pakistan journal of medical sciences
pub_type: 杂志文章
doi:10.12669/pjms.344.15521
更新日期:2018-07-01 00:00:00
abstract:OBJECTIVE:This study aims to determine expression of Glial Fibrillary Acidic Protein and of Alpha Smooth Muscle Actin (α-SMA) in hepatic stellate cells of CHC cases and their association with stage of fibrosis. METHODS:The study was conducted at Ziauddin University, Clifton Campus during the year 2010-2012. Sixty Chro...
journal_title:Pakistan journal of medical sciences
pub_type: 杂志文章
doi:10.12669/pjms.305.5534
更新日期:2014-09-01 00:00:00
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journal_title:Pakistan journal of medical sciences
pub_type: 杂志文章
doi:10.12669/pjms.336.13188
更新日期:2017-11-01 00:00:00
abstract:UNLABELLED:Objective : Esophageal stethoscope has the advantage of being non-invasive, easily placed and capability to monitor the heart sound. This study was designed to determine whether the ratio of S1 to S2 analyzed by esophageal stethoscope and wireless bluetooth transmission can be accurate indicator that express...
journal_title:Pakistan journal of medical sciences
pub_type: 杂志文章
doi:10.12669/pjms.294.3639
更新日期:2013-07-01 00:00:00
abstract:Objective:During COVID-19 pandemic, the institutions in Pakistan have started online learning. This study explores the perception of teachers and students regarding its advantages, limitations and recommendations. Methods:This qualitative case study was conducted from March to April 2020. Using maximum variation sampl...
journal_title:Pakistan journal of medical sciences
pub_type: 杂志文章
doi:10.12669/pjms.36.COVID19-S4.2785
更新日期:2020-05-01 00:00:00
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journal_title:Pakistan journal of medical sciences
pub_type:
doi:10.12669/pjms.292.2981
更新日期:2013-04-01 00:00:00