Abstract:
:The adaptor protein-2 (AP-2) complex refers to a class of proteins that play an important role in cargo recognition and are involved in clathrin-mediated endocytosis of cargo proteins from the plasma membrane in animal cells. However, there have been few studies of AP-2 in insects. Here, we isolated an AP-2 gene from Apis cerana cerana (A. cerana cerana) and explored the function of this gene, named AccAP2m. The obtained amino acid sequence of AccAP2m is highly conserved across species and contains conserved features of the AP-2 family. Quantitative real-time PCR analysis showed that AccAP2m mRNA levels were high in newly emerged workers, and the expression levels were higher in muscle than in other tissues. The expression of AccAP2m was altered in response to various environmental stress factors. Moreover, overexpression of recombinant AccAP2m protein enhanced the resistance of the bacteria to HgCl2 , CdCl2 , and cumyl hydroperoxide. In addition, after knockdown of AccAP2m in A. cerana cerana by RNA interference (RNAi), the transcription of some antioxidant genes was downregulated, and the activities of some antioxidant enzymes decreased. Taken together, these results indicate that AccAP2m may play an indispensable role in response to oxidative stresses.
journal_name
J Cell Biochemjournal_title
Journal of cellular biochemistryauthors
Wang X,Chao Y,Wang Y,Xu B,Wang C,Li Hdoi
10.1002/jcb.28919subject
Has Abstractpub_date
2019-10-01 00:00:00pages
16600-16613issue
10eissn
0730-2312issn
1097-4644journal_volume
120pub_type
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