Abstract:
:The aim of the present study was to extract and characterize bioactive components from separate body organs of Holothuria leucospilota. Preliminary qualitative assessment of the crude extracts was positive for phenols, terpenoids, carbohydrates, flavonoids, saponins, glycosides, cardiac glycosides, steroids, phlobatannins, and tannins in all body organs evaluated. Phenolics were the most abundant group of bioactives accounting for approximately 80%. The extraction solvent mixtures that yielded most compounds evaluated were methanol/acetone (3:1, v:v) and methanol/distilled water (3:1, v:v). In other analyses, GC-MS data revealed diverse metabolic and biologically active compounds, where those in high concentrations included 2-Pentanone, 4-hydroxy-4-methyl- among the ketones; phenol- 2,4-bis(1,1-dimethylethyl)-, a phenol group; and 2-Chlorooctane, a hydrocarbon. Among FA and their methyl/ethyl esters, n-hexadecanoic acid, 5,8,11,14-eicosatetraenoic acid ethyl ester (arachidonic acid), and 5,8,11,14,17-eicosapentaenoic acid methyl ester (EPA) were among the most abundant FAMEs accounting for approximately 50% of the subgroups measured. Data from GC-FID analysis revealed methyl laurate (C12:0), methyl myristate (C14:0), methyl palmitate (C16:0), and methyl stearate (18:0) methyl esters as the most abundant saturated FA, whereas cis-9-oleic methyl ester (C18:1) and methyl linoleate (C18:2) were found as the major monounsaturated FA and PUFA FAMEs, respectively, in the body wall of the species. Taken together, the extraction and characterization of different categories of metabolically and biologically active compounds in various organ extracts of H. leucospilota suggest that the species is potentially a rich source of cholesterol-lowering, antioxidant, antimicrobial, and anticancer agents. These substances are known to benefit human health and assist in disease prevention. These findings justify the use of sea cucumbers in traditional folklore medication and the current interest and attention focused on the species to mine for bioactives in new drugs research.
journal_name
Biomed Res Intjournal_title
BioMed research internationalauthors
Ceesay A,Nor Shamsudin M,Aliyu-Paiko M,Ismail IS,Nazarudin MF,Mohamed Alipiah Ndoi
10.1155/2019/2640684subject
Has Abstractpub_date
2019-04-15 00:00:00pages
2640684eissn
2314-6133issn
2314-6141journal_volume
2019pub_type
杂志文章abstract::Catalases are widely used in many scientific areas. A catalase gene (Kat) from Geobacillus sp. CHB1 encoding a monofunctional catalase was cloned and recombinant expressed in Escherichia coli (E. coli), which was the first time to clone and express this type of catalase of genus Geobacillus strains as far as we know. ...
journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2016/7535604
更新日期:2016-01-01 00:00:00
abstract::Deep venous thrombosis (DVT) constitutes a great threat to health worldwide. Endothelial cell injury and dysfunction comprise the critical contributor for the development of DVT. However, the mechanism behind it remains poorly elucidated. The study is aimed at investigating the role of microRNA-122 (miR-122) and oxida...
journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2020/9791608
更新日期:2020-10-27 00:00:00
abstract::In renal cell carcinoma, chromophobe renal cell carcinoma (ChRCC) is a distinct subtype, whose clinical manifestations often lack specificity, and the molecular mechanisms of ChRCC tumorigenesis remain generally vague. The target of this study was to discover novel biomarkers involved in ChRCC by integrated bioinforma...
journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2020/2671281
更新日期:2020-02-07 00:00:00
abstract:Objective:Mounting evidence has elaborated the implication of long noncoding RNAs (lncRNAs) in tumorigenesis of several cancers, including glioma. However, little was known about the mechanism of lncRNA maternally expressed gene 3 (MEG3) in the development and progression of glioma. This work is designed to explore the...
journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2020/4309161
更新日期:2020-01-11 00:00:00
abstract::Newcastle disease (ND) is one of the most devastating diseases that considerably cripple the global poultry industry. Because of its enormous socioeconomic importance and potential to rapidly spread to naïve birds in the vicinity, ND is included among the list of avian diseases that must be notified to the OIE immedia...
journal_title:BioMed research international
pub_type: 杂志文章,评审
doi:10.1155/2018/7278459
更新日期:2018-08-05 00:00:00
abstract:Objectives:This study aims to determine the changing in hip anatomy parameters with age and reveals the reason for the extorsion of lower extremity in the aged. Design:Retrospective study. Participants:One hundred and forty patients who had received imaging check of the femur and acetabulum between October 2013 and O...
journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2018/1507979
更新日期:2018-09-27 00:00:00
abstract::Polyketide belongs to a family of abundant natural products typically produced by the filamentous soil bacteria Streptomyces. Similar to the biosynthesis of most secondary metabolites produced in the Streptomyces species, polyketide compounds are synthesized through tight regulatory networks in the cell, and thus extr...
journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2013/549737
更新日期:2013-01-01 00:00:00
abstract::The purpose of this study was to assess the impact of 3 months of aromatase inhibition together with progestin add-back on ovarian endometrioma size. This prospective cohort study was performed at University Medical Center (UC San Diego). Women trying to conceive were excluded. After informed consent, all women were t...
journal_title:BioMed research international
pub_type: 临床试验,杂志文章
doi:10.1155/2015/878517
更新日期:2015-01-01 00:00:00
abstract:Objective:To investigate whether preventive administration of a proton pump inhibitor (PPI) can reduce the occurrence and development of traumatic granuloma (TG) following type IV-VI cordectomy. Methods:We retrospectively analyzed the status of postoperative granulomas in 37 patients who underwent type IV-VI cordectom...
journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2020/4178376
更新日期:2020-08-10 00:00:00
abstract::Sun Island Bund Wetland (SIBW) is a river floodplain wetland located at the south part of Heilongjiang Province in Northeast China. An investigation of the influence of habitat type on macroinvertebrates assemblages structure was conducted in July 2016. Nine (9) sampling sites were selected based on sediment type, wat...
journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2019/2650678
更新日期:2019-02-13 00:00:00
abstract::Diosmin has been widely used to treat patients with vascular pain for its potent anti-inflammatory and analgesic effects. To evaluate the therapeutic effects of Diosmin in the treatment of radicular pain, we conducted an investigator-initiated, randomized, active-controlled noninferiority trial between January 1, 2009...
journal_title:BioMed research international
pub_type: 杂志文章,随机对照试验
doi:10.1155/2017/6875968
更新日期:2017-01-01 00:00:00
abstract::Nutrient overload leads to impaired muscle oxidative capacity and insulin sensitivity. However, comparative analyses of the effects of dietary manipulation on skeletal muscles with different fiber composition are lacking. This study aimed to investigate the selective adaptations in the soleus and tibialis anterior mus...
journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2014/569623
更新日期:2014-01-01 00:00:00
abstract:OBJECTIVE:Identification of the factors that influence sedentary behaviour in older adults is important for the design of appropriate intervention strategies. In this study, we determined the prevalence of sedentary behaviour and its association with physical, cognitive, and psychosocial status among older adults resid...
journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2017/9160504
更新日期:2017-01-01 00:00:00
abstract:BACKGROUND:Physical activity is associated with reduced mortality and morbidity. Cardiac rehabilitation (CR) is an effective intervention for patients with cardiovascular disease (CVD). Unfortunately, women are less likely to engage in, or sustain, regular physical activity. Objectives were to (1) describe women's guid...
journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2013/121030
更新日期:2013-01-01 00:00:00
abstract:Objective:Epidemiological studies have shown a correlation between periodontal disease (PD) and age-related macular degeneration (AMD). However, the results have been inconsistent, and no relevant meta-analysis has been performed on this topic. Hence, we performed a meta-analysis to evaluate whether the two diseases ar...
journal_title:BioMed research international
pub_type: 杂志文章,评审
doi:10.1155/2020/4753645
更新日期:2020-09-28 00:00:00
abstract::Ablative therapies have been utilized with increasing frequency for the treatment of Barrett's esophagus with and without dysplasia. Multiple modalities are available for topical ablation of the esophagus, but radiofrequency ablation (RFA) remains the most commonly used. There have been significant advances in techniq...
journal_title:BioMed research international
pub_type: 杂志文章,评审
doi:10.1155/2014/642063
更新日期:2014-01-01 00:00:00
abstract::Dental modeling resins have been developed for use in areas where highly precise resin structures are needed. The manufacturers claim that these polymethyl methacrylate/methyl methacrylate (PMMA/MMA) resins show little or no shrinkage after polymerization. This study examined the polymerization shrinkage of five denta...
journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2014/914739
更新日期:2014-01-01 00:00:00
abstract::After pancreatoduodenectomy, complication rates are up to 40%. To predict the risk of developing postoperative pancreatic fistula or severe complications, various factors were evaluated. 110 consecutive patients undergoing pancreatoduodenectomy at our institute between January 2012 and September 2014 with complete CT ...
journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2015/824525
更新日期:2015-01-01 00:00:00
abstract::Unipotent spermatogonial stem cells (SSCs) can be transformed into ESC-like cells that exhibit pluripotency in vitro. However, except for mouse models, their characterization and their origins have remained controversies in other models including humans. This controversy has arisen primarily from the lack of the direc...
journal_title:BioMed research international
pub_type: 临床试验,杂志文章
doi:10.1155/2013/143028
更新日期:2013-01-01 00:00:00
abstract:Aim:The aim of this study was to explore the correlation of chemokine receptor CXCR3 with M2 macrophage infiltration, various clinicopathological features, and prognosis in patients diagnosed with gastric cancer (GC). Methods:Expression of CXCR3 protein and M2 macrophage was evaluated in 156 GC patients and correspond...
journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2019/6832867
更新日期:2019-05-21 00:00:00
abstract::Carbonic anhydrases (CAs) are enzymes that catalyse the reversible hydration of CO2 to bicarbonate. CA XII is considered a potential biomarker of tumor cells and a promising target for specific therapies. The aim of the current study was to develop new monoclonal antibodies (MAbs) against human recombinant CA XII and ...
journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2014/309307
更新日期:2014-01-01 00:00:00
abstract:PURPOSE:In order to establish inspection routines for individual intraocular lenses (IOLs), their surfaces have to be measured separately. Currently available measurement devices lack this functionality. The purpose of this study is to evaluate a new topography measurement device based on wavefront analysis for measuri...
journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2013/363742
更新日期:2013-01-01 00:00:00
abstract::The early diagnosis of Alzheimer's disease (AD) and mild cognitive impairment (MCI) is very important for treatment research and patient care purposes. Few biomarkers are currently considered in clinical settings, and their use is still optional. The objective of this work was to determine whether multimodal and nonpr...
journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2015/961314
更新日期:2015-01-01 00:00:00
abstract::Sarcopenia is defined as a syndrome characterized by progressive and generalized loss of muscle mass and strength. The more rationale approach to delay the progression of sarcopenia is based on the combination of proper nutrition, possibly associated with the use of dietary supplements and a regular exercise program. ...
journal_title:BioMed research international
pub_type: 杂志文章,评审
doi:10.1155/2015/524948
更新日期:2015-01-01 00:00:00
abstract::Destruction of extracellular matrix (ECM) is one of the basic steps of tumor invasion and metastasis. Matrix metalloproteinase (MMP) 9, a kind of zinc-ion-dependent endopeptidase, can degrade almost all protein components in the ECM, destroy the histological barrier of tumor cell invasion, and play a key role in tumor...
journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2020/4350783
更新日期:2020-04-15 00:00:00
abstract::Although the dynamic motions and peptidyl transferase activity seem to be embedded in the rRNAs, the ribosome contains more than 50 ribosomal proteins (r-proteins), whose functions remain largely elusive. Also, the precise forms of some of these r-proteins, as being part of the ribosome, are not structurally solved du...
journal_title:BioMed research international
pub_type: 杂志文章,评审
doi:10.1155/2015/539238
更新日期:2015-01-01 00:00:00
abstract::The objective of this study was to investigate the effects of selective inducible nitric oxide synthase and neuronal nitric oxide synthase inhibitors on cartilage regeneration. The study involved 27 Wistar rats that were divided into five groups. On Day 1, both knees of 3 rats were resected and placed in a formalin so...
journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2016/7857345
更新日期:2016-01-01 00:00:00
abstract::[This corrects the article DOI: 10.1155/2020/4671349.]. ...
journal_title:BioMed research international
pub_type: 杂志文章,已发布勘误
doi:10.1155/2020/7638969
更新日期:2020-06-30 00:00:00
abstract::Heart failure (HF) can be defined as cardiac structural or functional abnormality leading to a series of symptoms due to deficiency of oxygen delivery. In the clinical practice, acute heart failure (AHF) is usually performed as cardiogenic shock (CS), pulmonary edema, and single or double ventricle congestive heart fa...
journal_title:BioMed research international
pub_type: 杂志文章,评审
doi:10.1155/2016/7263187
更新日期:2016-01-01 00:00:00
abstract::Pinelliae Rhizoma Praeparatum Cum Alumine (PRPCA) is useful for eliminating dampness and phlegm in clinical settings, targeting the main mechanisms of insomnia as defined in traditional Chinese medicine. However, little is known regarding the sedative and hypnotic effects of PRPCA. In the present study, we examined th...
journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2019/6198067
更新日期:2019-04-28 00:00:00