mSphere of Influence: the Power of Yeast Genetics Still Going Strong!

abstract：:Zoonosis is the leading cause of emerging infectious diseases. In a recent article, R. S. Shabman et al. (mSphere 1[1]:e00070-15, 2016, http://dx.doi.org/10.1128/mSphere.00070-15) report the identification of a novel gammaherpesvirus in a cell line derived from the microbat Myotis velifer incautus. This is the first r...

journal_title：mSphere

authors： Host KM,Damania B

更新日期：2016-02-17 00:00:00

abstract：:Neal Hammer works in the field of bacterial pathogenesis, metabolism, and antibiotic resistance. In this mSphere of Influence article, he reflects on how "Gut inflammation provides a respiratory electron acceptor for Salmonella" by Winter and colleagues (S. E. Winter, P. Thiennimitr, M. G. Winter, B. P. Butler, et al....

journal_title：mSphere

authors： Hammer ND

更新日期：2020-02-26 00:00:00

abstract：:Current in vitro techniques for the culture of microorganisms, and particularly of delicate microbial biofilms, are still mostly limited to low-density plates and manual labor and are not amenable to automation and true high-throughput (HT) applications. We have developed a novel fully automated platform for the forma...

journal_title：mSphere

authors： Srinivasan A,Torres NS,Leung KP,Lopez-Ribot JL,Ramasubramanian AK

更新日期：2017-06-28 00:00:00

abstract：:This study examines the pharmacodynamics of antimicrobials that are used to treat Salmonella with each other and with key components of the innate immune system. Antimicrobial synergy was assessed using time-kill and checkerboard assays. Antimicrobial interactions with innate immunity were studied by employing catheli...

journal_title：mSphere

authors： Sakoulas G,Kumaraswamy M,Kousha A,Nizet V

更新日期：2017-12-06 00:00:00

abstract：:Female Hawaiian bobtail squid, Euprymna scolopes, harbor a symbiotic bacterial community in a reproductive organ, the accessory nidamental gland (ANG). This community is known to be stable over several generations of wild-caught bobtail squid but has, to date, been examined for only one population in Maunalua Bay, Oah...

journal_title：mSphere

authors： Kerwin AH,Nyholm SV

更新日期：2018-03-28 00:00:00

abstract：:Syndiniales are a ubiquitous group of protist parasites that infect and kill a wide range of hosts, including harmful bloom-forming dinoflagellates. Despite the importance of parasitism as an agent of plankton mortality, parasite-host dynamics remain poorly understood, especially over time, hindering the inclusion of ...

journal_title：mSphere

authors： Anderson SR,Harvey EL

更新日期：2020-05-27 00:00:00

abstract：:Increasing evidence shows that exosomes are key regulators in cancer cell-to-cell communication. Several reports on Epstein-Barr virus (EBV)-related malignancies demonstrate that latent membrane protein 1 (LMP1) secreted by exosomes derived from EBV- or LMP1-positive cells can promote cancer progression and metastasis...

journal_title：mSphere

authors： Kobayashi E,Aga M,Kondo S,Whitehurst C,Yoshizaki T,Pagano JS,Shackelford J

更新日期：2018-02-07 00:00:00

abstract：:Bifidobacterium species are important commensals capable of dominating the infant gut microbiome, in part by producing acids that suppress growth of other taxa. Bifidobacterium species are less prone to possessing antimicrobial resistance (AMR) genes (ARGs) than other taxa that may colonize infants. Given that AMR is ...

journal_title：mSphere

authors： Taft DH,Liu J,Maldonado-Gomez MX,Akre S,Huda MN,Ahmad SM,Stephensen CB,Mills DA

更新日期：2018-09-26 00:00:00

abstract：:Bacteria must sense alterations in their environment and respond with changes in function and/or structure in order to cope. Extracytoplasmic function sigma factors (ECF σs) modulate transcription in response to cellular and environmental signals. The symbiotic nitrogen-fixing alphaproteobacterium Sinorhizobium melilo...

journal_title：mSphere

authors： Lang C,Barnett MJ,Fisher RF,Smith LS,Diodati ME,Long SR

更新日期：2018-10-10 00:00:00

abstract：:The genetic stability of every living organism depends on accurate DNA replication and repair systems. Here, we investigated the Aspergillus fumigatusMSH2 mismatch repair (MMR) gene MshA and how it impacts virulence and the evolution of azole resistance. We examined mshA gene variation in 62 environmental and clinical...

journal_title：mSphere

authors： Dos Reis TF,Silva LP,de Castro PA,do Carmo RA,Marini MM,da Silveira JF,Ferreira BH,Rodrigues F,Lind AL,Rokas A,Goldman GH

更新日期：2019-08-07 00:00:00

abstract：:Antibiotic resistance is a global crisis that threatens our ability to treat bacterial infections, such as tuberculosis, caused by Mycobacterium tuberculosis Of the 10 million cases of tuberculosis in 2017, approximately 19% of new cases and 43% of previously treated cases were caused by strains of M. tuberculosis res...

journal_title：mSphere

authors： Harrison GA,Mayer Bridwell AE,Singh M,Jayaraman K,Weiss LA,Kinsella RL,Aneke JS,Flentie K,Schene ME,Gaggioli M,Solomon SD,Wildman SA,Meyers MJ,Stallings CL

更新日期：2019-09-11 00:00:00

abstract：:Infections by malaria parasites can lead to very different clinical outcomes, ranging from mild symptoms to death. Differences in the ability of the spleen to deal with the infected red blood cells (iRBCs) are linked to differences in virulence. Using virulent and avirulent strains of the rodent malaria parasite Plasm...

journal_title：mSphere

authors： Huang X,Huang S,Ong LC,Lim JC,Hurst RJ,Mushunje AT,Matsudaira PT,Han J,Preiser PR

更新日期：2015-12-09 00:00:00

abstract：:Nonribosomal peptide synthases produce short peptides in a manner that is distinct from classical mRNA-dependent ribosome-mediated translation. The Mycobacterium tuberculosis genome harbors a nonribosomal peptide synthase gene, nrp, which is part of a gene cluster proposed to be involved in the biosynthesis of isonitr...

journal_title：mSphere

authors： Bhatt K,Machado H,Osório NS,Sousa J,Cardoso F,Magalhães C,Chen B,Chen M,Kim J,Singh A,Ferreira CM,Castro AG,Torrado E,Jacobs WR Jr,Bhatt A,Saraiva M

更新日期：2018-10-31 00:00:00

abstract：:Type I interferons (IFN) initiate an antiviral state through a signal transduction cascade that leads to the induction of hundreds of IFN-stimulated genes (ISGs) to restrict viral infection. Recently, RNA modifications on both host and viral RNAs have been described as regulators of infection. However, the impact of h...

journal_title：mSphere

authors： Williams GD,Gokhale NS,Snider DL,Horner SM

更新日期：2020-05-13 00:00:00

abstract：:Antibiotic resistance has become a major public health concern as bacteria evolve to evade drugs, leading to recurring infections and a decrease in antibiotic efficacy. Systematic efforts have revealed mechanisms involved in resistance. Yet, in many cases, how these specific mechanisms accelerate or slow the evolution...

journal_title：mSphere

authors： Langevin AM,El Meouche I,Dunlop MJ

更新日期：2020-12-16 00:00:00

abstract：:Control and eradication of foot-and-mouth disease (FMD) are impeded by the existence of a persistent, subclinical phase of infection in ruminants; animals with this status are referred to as carriers. However, the epidemiological significance of these FMD virus (FMDV) carriers is uncertain. In the current investigatio...

journal_title：mSphere

authors： Arzt J,Belsham GJ,Lohse L,Bøtner A,Stenfeldt C

更新日期：2018-09-12 00:00:00

abstract：:The Enterobacterales order of Gram-negative bacteria includes the common nosocomial pathogens Klebsiella pneumoniae, Escherichia coli, Serratia marcescens, and Enterobacter species. Intestinal domination by some colonizing bacterial taxa is associated with subsequent infection, but 16S rRNA gene sequencing is too cost...

journal_title：mSphere

authors： Rao K,Seekatz A,Bassis C,Sun Y,Mantlo E,Bachman MA

更新日期：2020-07-22 00:00:00

abstract：:Feedback mechanisms are fundamental to the control of physiological responses. One important example in gene regulation, termed negative autoregulation (NAR), occurs when a transcription factor (TF) inhibits its own production through transcriptional repression. This enables more-rapid homeostatic control of gene expr...

journal_title：mSphere

authors： Kozuch BC,Shaffer MG,Culyba MJ

更新日期：2020-08-19 00:00:00

abstract：:Brucella, the agent causing brucellosis, is a major zoonotic pathogen with worldwide distribution. Brucella resides and replicates inside infected host cells in membrane-bound compartments called Brucella-containing vacuoles (BCVs). Following uptake, Brucella resides in endosomal BCVs (eBCVs) that gradually mature fro...

journal_title：mSphere

authors： Casanova A,Low SH,Québatte M,Sedzicki J,Tschon T,Ketterer M,Smith K,Emmenlauer M,Ben-Tekaya H,Dehio C

更新日期：2019-06-26 00:00:00

abstract：:Escherichia coli populations undergo repeated replacement of parental genotypes with fitter variants deep in stationary phase. We isolated one such variant, which emerged after 3 weeks of maintaining an E. coli K-12 population in stationary phase. This variant displayed a small colony phenotype and slow growth and was...

journal_title：mSphere

authors： Nandy P,Chib S,Seshasayee A

更新日期：2020-04-15 00:00:00

abstract：:Globally, more antimicrobials are used in food-producing animals than in humans, and the extensive use of medically important human antimicrobials poses a significant public health threat in the face of rising antimicrobial resistance (AMR). The development of novel ionophores, a class of antimicrobials used exclusive...

journal_title：mSphere

authors： Harbison-Price N,Ferguson SA,Heikal A,Taiaroa G,Hards K,Nakatani Y,Rennison D,Brimble MA,El-Deeb IM,Bohlmann L,McDevitt CA,von Itzstein M,Walker MJ,Cook GM

更新日期：2020-03-18 00:00:00

abstract：:Information on severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) spread in Africa is limited by insufficient diagnostic capacity. Here, we assessed the coronavirus disease (COVID-19)-related diagnostic workload during the onset of the pandemic in the central laboratory of Benin, Western Africa; characterize...

journal_title：mSphere

authors： Sander AL,Yadouleton A,Moreira-Soto A,Tchibozo C,Hounkanrin G,Badou Y,Fischer C,Krause N,Akogbeto P,F de Oliveira Filho E,Dossou A,Brünink S,Drosten C,Aïssi MAJ,Harouna Djingarey M,Hounkpatin B,Nagel M,Drexler JF

更新日期：2021-01-13 00:00:00

abstract：:Sepsis caused by Gram-negative bacteria is the consequence of an unrestrained infection that continuously releases lipopolysaccharide (LPS) into the bloodstream, which triggers an uncontrolled systemic inflammatory response leading to multiorgan failure and death. After scrutinizing the immune modulation exerted by a ...

journal_title：mSphere

authors： Ramos-Benitez MJ,Ruiz-Jimenez C,Rosado-Franco JJ,Ramos-Pérez WD,Mendez LB,Osuna A,Espino AM

更新日期：2018-12-19 00:00:00

abstract：:Malaria remains one of the most devastating parasitic diseases worldwide, with 90% of the malaria deaths in Africa in 2013 attributable to Plasmodium falciparum. The clinical symptoms of malaria include cycles of fever, corresponding to parasite rupture from red blood cells every 48 h. Parasite pathways involved in th...

journal_title：mSphere

authors： Thomas P,Sedillo J,Oberstaller J,Li S,Zhang M,Singh N,Wang CC,Udenze K,Jiang RH,Adams JH

更新日期：2016-10-26 00:00:00

abstract：:Clostridioides difficile is an obligately anaerobic, spore-forming, Gram-positive pathogenic bacterium that is considered the leading cause of nosocomial diarrhea worldwide. Recent studies have attempted to understand the biology of the outermost layer of C. difficile spores, the exosporium, which is believed to contr...

journal_title：mSphere

authors： Romero-Rodríguez A,Troncoso-Cotal S,Guerrero-Araya E,Paredes-Sabja D

更新日期：2020-11-18 00:00:00

abstract：:The acquisition of genetic material conferring the arsenal necessary for host virulence is a prerequisite on the path to becoming a plant pathogen. More subtle mutations are also required for the perception of cues signifying the presence of the target host and optimal conditions for colonization. The decision to acti...

journal_title：mSphere

authors： Jourdan S,Francis IM,Deflandre B,Loria R,Rigali S

更新日期：2017-03-01 00:00:00

abstract：:Buruli ulcer is a neglected tropical disease of skin and subcutaneous tissue caused by infection with the pathogen Mycobacterium ulcerans Many critical issues for disease control, such as understanding the mode of transmission and identifying source reservoirs of M. ulcerans, are still largely unknown. Here, we used g...

journal_title：mSphere

authors： Vandelannoote K,Phanzu DM,Kibadi K,Eddyani M,Meehan CJ,Jordaens K,Leirs H,Portaels F,Stinear TP,Harris SR,de Jong BC

更新日期：2019-02-06 00:00:00

abstract：:Amino acids participate in several critical processes in the biology of trypanosomatids, such as osmoregulation, cell differentiation, and host cell invasion. Some of them provide reducing power for mitochondrial ATP synthesis. It was previously shown that alanine, which is formed mainly by the amination of pyruvate, ...

journal_title：mSphere

authors： Girard RMBM,Crispim M,Alencar MB,Silber AM

更新日期：2018-07-18 00:00:00

abstract：:Diana V. Pastrana works in the field of DNA tumor virus biology. In this mSphere of Influence article, she reflects on how the two papers "Donor origin of BKV replication after kidney transplantation" (C. Schmitt, L. Raggub, S. Linnenweber-Held, O. Adams, et al., J Clin Virol 59:120-125, 2014, https://doi.org/10.1016/...

journal_title：mSphere

authors： Pastrana DV

更新日期：2020-01-15 00:00:00

abstract：:Glycine serves as a major source of single carbon units for biochemical reactions within bacterial cells. Utilization of glycine is tightly regulated and revolves around a key group of proteins known as the glycine cleavage system (GCS). Our lab previously identified the transcriptional regulator GcsR (PA2449) as bein...

journal_title：mSphere

authors： Sarwar Z,Lundgren BR,Grassa MT,Wang MX,Gribble M,Moffat JF,Nomura CT

更新日期：2016-04-27 00:00:00