Pneumococcal VncR Strain-Specifically Regulates Capsule Polysaccharide Synthesis.

Abstract:

:Capsular polysaccharides (CPS), a major virulence factor in Streptococcus pneumoniae, become thicker during blood invasion while not during asymptomatic nasopharyngeal colonization. However, the underlying mechanism controlling this differential pneumococcal CPS regulation remain unclear. Here, we show how VncR, the response regulator of the vancomycin resistance locus (vncRS operon), regulates CPS expression in vncR mutants in three serotype (type 2, 3, and 6B) backgrounds upon exposure to serum lactoferrin (LF). Comparative analysis of CPS levels in the wild type (WT) of three strains and their isogenic vncR mutants after LF exposure revealed a strain-specific alteration in CPS production. Consistently, VncR-mediated strain-specific CPS production is correlated with pneumococcal virulence, in vivo. Electrophoretic mobility-shift assay and co-immunoprecipitation revealed an interaction between VncR and the cps promoter (cpsp) in the presence of serum. In addition, in silico analysis uncovered this protein-DNA interaction, suggesting that VncR binds with the cpsp, and recognizes the strain-specific significance of the tandem repeats in cpsp. Taken together, the interaction of VncR and cpsp after serum exposure plays an essential role in regulating differential strain-specific CPS production, which subsequently determines strain-specific systemic virulence. This study highlights how host protein LF contributes to pneumococcal VncR-mediated CPS production. As CPS plays a significant role in immune evasion, these findings suggest that drugs designed to interrupt the VncR-mediated CPS production could help to combat pneumococcal infections.

journal_name

Front Microbiol

authors

Ghosh P,Shah M,Ravichandran S,Park SS,Iqbal H,Choi S,Kim KK,Rhee DK

doi

10.3389/fmicb.2019.02279

subject

Has Abstract

pub_date

2019-10-01 00:00:00

pages

2279

issn

1664-302X

journal_volume

10

pub_type

杂志文章
  • Dual Repressive Function by Cip1, a Budding Yeast Analog of p21, in Cell-Cycle START Regulation.

    abstract::Cip1, a newly identified yeast analog of p21, is a Cln3-CDK inhibitor that negatively regulates cell-cycle START. However, its function remains poorly understood. In this study, we found that deletion of CLN3 did not result in bypass of G1-phase arrest caused by Cip1 overexpression. Cip1 depletion in cln3-null mutants...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2020.01623

    authors: Li P,Liu X,Hao Z,Jia Y,Zhao X,Xie D,Dong J,Zeng F

    更新日期:2020-07-09 00:00:00

  • Detection of autotrophic verrucomicrobial methanotrophs in a geothermal environment using stable isotope probing.

    abstract::Genomic analysis of the methanotrophic verrucomicrobium "Methylacidiphilum infernorum" strain V4 has shown that most pathways conferring its methanotrophic lifestyle are similar to those found in proteobacterial methanotrophs. However, due to the large sequence divergence of its methane monooxygenase-encoding genes (p...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2012.00303

    authors: Sharp CE,Stott MB,Dunfield PF

    更新日期:2012-08-17 00:00:00

  • Decoding the Role of Glycans in Malaria.

    abstract::Complications arising from malaria are a concern for public health authorities worldwide, since the annual caseload in humans usually exceeds millions. Of more than 160 species of Plasmodium, only 4 infect humans, with the most severe cases ascribed to Plasmodium falciparum and the most prevalent to Plasmodium vivax. ...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章,评审

    doi:10.3389/fmicb.2017.01071

    authors: Gomes PS,Feijó DF,Morrot A,Freire-de-Lima CG

    更新日期:2017-06-09 00:00:00

  • Solvent and Water Mediated Structural Variations in Deoxynivalenol and Their Potential Implications on the Disruption of Ribosomal Function.

    abstract::Fusarium head blight (FHB) is a disease of cereal crops caused by trichothecene producing Fusarium species. Trichothecenes, macrocylicic fungal metabolites composed of three fused rings (A-C) with one epoxide functionality, are a class of mycotoxins known to inhibit protein synthesis in eukaryotic ribosomes. These tox...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2016.01239

    authors: Foroud NA,Shank RA,Kiss D,Eudes F,Hazendonk P

    更新日期:2016-08-17 00:00:00

  • Saccharomyces cerevisiae - Insects Association: Impacts, Biogeography, and Extent.

    abstract::Over the last few years, an increasing number of studies have reported the existence of an association between the budding yeast Saccharomyces cerevisiae and insects. The discovery of this relationship has called into question the hypothesis that S. cerevisiae is unable to survive in nature and that the presence of S....

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章,评审

    doi:10.3389/fmicb.2020.01629

    authors: Meriggi N,Di Paola M,Cavalieri D,Stefanini I

    更新日期:2020-07-14 00:00:00

  • Resistance of Animal Strains of Pseudomonas aeruginosa to Carbapenems.

    abstract::Carbapenems are major antibiotics reserved to human medicine. This study aimed to investigate the mechanisms of carbapenem resistance of a selection of Pseudomonas aeruginosa veterinary strains from the French network Resapath. Thirty (5.7%) imipenem and/or meropenem non-susceptible P. aeruginosa of canine (n = 24), f...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2017.01847

    authors: Haenni M,Bour M,Châtre P,Madec JY,Plésiat P,Jeannot K

    更新日期:2017-09-29 00:00:00

  • Antimicrobial Protection of Marsupial Pouch Young.

    abstract::Marsupials diverged from eutherian mammals about 148 million years ago and represent a unique lineage of mammals with distinctive morphological and reproductive characteristics. Marsupials have significantly shorter gestation periods than eutherians. Pregnancy typically ranges from 15 to 35 days, with young being born...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章,评审

    doi:10.3389/fmicb.2017.00354

    authors: Cheng Y,Belov K

    更新日期:2017-03-07 00:00:00

  • Comparative Genomics Reveals Specific Genetic Architectures in Nicotine Metabolism of Pseudomonas sp. JY-Q.

    abstract::Microbial degradation of nicotine is an important process to control nicotine residues in the aqueous environment. In this study, a high active nicotine degradation strain named Pseudomonas sp. JY-Q was isolated from tobacco waste extract (TWE). This strain could completely degrade 5.0 g l-1 nicotine in 24 h under opt...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2017.02085

    authors: Li J,Qian S,Xiong L,Zhu C,Shu M,Wang J,Jiao Y,He H,Zhang F,Linhardt RJ,Zhong W

    更新日期:2017-10-31 00:00:00

  • The Human Gastric Microbiome Is Predicated upon Infection with Helicobacter pylori.

    abstract::The human gastric lumen is one of the most hostile environments of the human body suspected to be sterile until the discovery of Helicobacter pylori (H.p.). State of the art next generation sequencing technologies multiply the knowledge on H.p. functional genomics as well as on the colonization of supposed sterile hum...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2017.02508

    authors: Klymiuk I,Bilgilier C,Stadlmann A,Thannesberger J,Kastner MT,Högenauer C,Püspök A,Biowski-Frotz S,Schrutka-Kölbl C,Thallinger GG,Steininger C

    更新日期:2017-12-14 00:00:00

  • Genome-Wide Analysis of Salicylate and Dibenzofuran Metabolism in Sphingomonas Wittichii RW1.

    abstract::Sphingomonas wittichii RW1 is a bacterium isolated for its ability to degrade the xenobiotic compounds dibenzodioxin and dibenzofuran (DBF). A number of genes involved in DBF degradation have been previously characterized, such as the dxn cluster, dbfB, and the electron transfer components fdx1, fdx3, and redA2. Here ...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2012.00300

    authors: Coronado E,Roggo C,Johnson DR,van der Meer JR

    更新日期:2012-08-23 00:00:00

  • Mass Spectrometry Guided Discovery and Design of Novel Asperphenamate Analogs From Penicillium astrolabium Reveals an Extraordinary NRPS Flexibility.

    abstract::Asperphenamate is a small peptide natural product that has gained much interest due to its antitumor activity. In the recent years numerous bioactive synthetic asperphenamate analogs have been reported, whereas only a handful of natural analogs either of microbial or plant origin has been discovered. Herein we describ...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2020.618730

    authors: Subko K,Wang X,Nielsen FH,Isbrandt T,Gotfredsen CH,Ramos MC,Mackenzie T,Vicente F,Genilloud O,Frisvad JC,Larsen TO

    更新日期:2021-01-15 00:00:00

  • Extracellular DNA metabolism in Haloferax volcanii.

    abstract::Extracellular DNA is found in all environments and is a dynamic component of the microbial ecosystem. Microbial cells produce and interact with extracellular DNA through many endogenous mechanisms. Extracellular DNA is processed and internalized for use as genetic information and as a major source of macronutrients, a...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2014.00057

    authors: Chimileski S,Dolas K,Naor A,Gophna U,Papke RT

    更新日期:2014-02-20 00:00:00

  • Stimulation of the Internal Ribosome Entry Site (IRES)-Dependent Translation of Enterovirus 71 by DDX3X RNA Helicase and Viral 2A and 3C Proteases.

    abstract::The translation of enterovirus 71 (EV71) is mediated by an internal ribosome entry site (IRES)-dependent manner. EV71 IRES comprises five highly structured domains (domains II-VI) in the 5'-untranslated region of the viral mRNA. A conserved AUG triplet residing in domain VI is proposed to be the ribosome entry site. I...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2018.01324

    authors: Su YS,Tsai AH,Ho YF,Huang SY,Liu YC,Hwang LH

    更新日期:2018-06-19 00:00:00

  • Comparative Analyses of the Digestive Tract Microbiota of New Guinean Passerine Birds.

    abstract::The digestive tract microbiota (DTM) plays a plethora of functions that enable hosts to exploit novel niches. However, our understanding of the DTM of birds, particularly passerines, and the turnover of microbial communities along the digestive tract are limited. To better understand how passerine DTMs are assembled, ...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2018.01830

    authors: Bodawatta KH,Sam K,Jønsson KA,Poulsen M

    更新日期:2018-08-10 00:00:00

  • Biophysical methods for the study of microbial surfaces.

    abstract::The challenge in studying the surface architecture of different microbial pathogens is to integrate the most current biochemical, spectroscopic, microscopic, and processing techniques. Individually these methods have insufficient sensitivity to reveal complex structures, such as branched, large, viscous polymers with ...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2011.00207

    authors: Frases S,Viana NB,Casadevall A

    更新日期:2011-10-10 00:00:00

  • Wasted Bread as Substrate for the Cultivation of Starters for the Food Industry.

    abstract::The amount of bread wasted daily worldwide, throughout its entire lifecycle, from production to distribution, is estimated to be hundreds of tons, therefore representing both economic and environmental issues. This work aimed at the valorization of wasted bread, setting-up a protocol for obtaining a growth medium to b...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2020.00293

    authors: Verni M,Minisci A,Convertino S,Nionelli L,Rizzello CG

    更新日期:2020-02-28 00:00:00

  • Chloroplast symbiosis in a marine ciliate: ecophysiology and the risks and rewards of hosting foreign organelles.

    abstract::Simultaneous use of both heterotrophic and autotrophic metabolism ("mixotrophy") is common among protists. Strombidium rassoulzadegani is a planktonic mixotrophic marine ciliate that saves chloroplasts from its algal food and obtains a nutritional subsidy via photosynthesis. Cultures from the northeast, northwest, and...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2012.00321

    authors: McManus GB,Schoener DM,Haberlandt K

    更新日期:2012-09-04 00:00:00

  • Soil Bacterial Function Associated With Stylo (Legume) and Bahiagrass (Grass) Is Affected More Strongly by Soil Chemical Property Than by Bacterial Community Composition.

    abstract::Soil microbes are driver of nutrient cycling, with microbial function affected by community composition and soil chemical property. Legume and grass are ubiquitous in many ecosystems, however, their differential effects on microbial function are less understood. Here we constructed compartmented rhizobox planted with ...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2019.00798

    authors: Zhou Y,Qin Y,Liu X,Feng Z,Zhu H,Yao Q

    更新日期:2019-04-12 00:00:00

  • Antimicrobial active herbal compounds against Acinetobacter baumannii and other pathogens.

    abstract::Bacterial pathogens cause a number of lethal diseases. Opportunistic bacterial pathogens grouped into ESKAPE pathogens that are linked to the high degree of morbidity, mortality and increased costs as described by Infectious Disease Society of America. Acinetobacter baumannii is one of the ESKAPE pathogens which cause...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章,评审

    doi:10.3389/fmicb.2015.00618

    authors: Tiwari V,Roy R,Tiwari M

    更新日期:2015-06-18 00:00:00

  • Corrigendum: Integrating DNA Methylation and Gene Expression Data in the Development of the Soybean-Bradyrhizobium N2-Fixing Symbiosis.

    abstract::[This corrects the article on p. 518 in vol. 7, PMID: 27148207.]. ...

    journal_title:Frontiers in microbiology

    pub_type: 已发布勘误

    doi:10.3389/fmicb.2016.00952

    authors: Davis-Richardson AG,Russell JT,Dias R,McKinlay AJ,Canepa R,Fagen JR,Rusoff KT,Drew JC,Kolaczkowski B,Emerich DW,Triplett EW

    更新日期:2016-06-15 00:00:00

  • TaqMan qPCR for Quantification of Clonostachys rosea Used as a Biological Control Agent Against Fusarium graminearum.

    abstract::Clonostachys rosea is a biological control agent against Fusarium graminearum in small grain cereals and maize. Infections with F. graminearum do not only reduce the yield but, due to the production of mycotoxins, also affect the entire value chain of food and feed. In addition, production of other secondary metabolit...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2019.01627

    authors: Gimeno A,Sohlberg E,Pakula T,Limnell J,Keller B,Laitila A,Vogelgsang S

    更新日期:2019-07-16 00:00:00

  • Molecular Characteristics of First IMP-4-Producing Enterobacter cloacae Sequence Type 74 and 194 in Korea.

    abstract::The worldwide dissemination of carbapenemase-producing Enterobacteriaceae (CPE) has become a major therapeutic concern in clinical settings. Enterobacter cloacae is a major pathogen that causes serious hospital-acquired infections. We investigated the clinical characteristics and molecular mechanisms of the first IMP-...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2017.02343

    authors: Lee JH,Bae IK,Lee CH,Jeong S

    更新日期:2017-11-28 00:00:00

  • Biphenyl Modulates the Expression and Function of Respiratory Oxidases in the Polychlorinated-Biphenyls Degrader Pseudomonas pseudoalcaligenes KF707.

    abstract::Pseudomonas pseudoalcaligenes KF707 is a soil bacterium which is known for its capacity to aerobically degrade harmful organic compounds such as polychlorinated biphenyls (PCBs) using biphenyl as co-metabolite. Here we provide the first genetic and functional analysis of the KF707 respiratory terminal oxidases in cell...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2017.01223

    authors: Sandri F,Fedi S,Cappelletti M,Calabrese FM,Turner RJ,Zannoni D

    更新日期:2017-06-30 00:00:00

  • Increased Denitrification Rates Associated with Shifts in Prokaryotic Community Composition Caused by Varying Hydrologic Connectivity.

    abstract::While modern developments in agriculture have allowed for increases in crop yields and rapid human population growth, they have also drastically altered biogeochemical cycles, including the biotransformation of nitrogen. Denitrification is a critical process performed by bacteria and fungi that removes nitrate in surf...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2017.02304

    authors: Tomasek A,Staley C,Wang P,Kaiser T,Lurndahl N,Kozarek JL,Hondzo M,Sadowsky MJ

    更新日期:2017-11-22 00:00:00

  • Bacterial Active Community Cycling in Response to Solar Radiation and Their Influence on Nutrient Changes in a High-Altitude Wetland.

    abstract::Microbial communities inhabiting high-altitude spring ecosystems are subjected to extreme changes in solar irradiance and temperature throughout the diel cycle. Here, using 16S rRNA gene tag pyrosequencing (cDNA) we determined the composition of actively transcribing bacteria from spring waters experimentally exposed ...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2016.01823

    authors: Molina V,Hernández K,Dorador C,Eissler Y,Hengst M,Pérez V,Harrod C

    更新日期:2016-11-17 00:00:00

  • Tet(C) Gene Transfer between Chlamydia suis Strains Occurs by Homologous Recombination after Co-infection: Implications for Spread of Tetracycline-Resistance among Chlamydiaceae.

    abstract::Chlamydia suis is a swine pathogen that has also recently been found to cause zoonotic infections of the human eye, pharynx, and gastrointestinal tract. Many strains contain a tetracycline class C gene [tet(C)] cassette that confers tetracycline resistance. The cassette was likely originally acquired by horizontal gen...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2017.00156

    authors: Marti H,Kim H,Joseph SJ,Dojiri S,Read TD,Dean D

    更新日期:2017-02-07 00:00:00

  • Phylodynamic analysis of a viral infection network.

    abstract::Viral infections by sexual and droplet transmission routes typically spread through a complex host-to-host contact network. Clarifying the transmission network and epidemiological parameters affecting the variations and dynamics of a specific pathogen is a major issue in the control of infectious diseases. However, co...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2012.00278

    authors: Shiino T

    更新日期:2012-07-31 00:00:00

  • Transcriptome analyses to investigate symbiotic relationships between marine protists.

    abstract::Rhizaria are an important component of oceanic plankton communities worldwide. A number of species harbor eukaryotic microalgal symbionts, which are horizontally acquired in the environment at each generation. Although these photosymbioses are determinant for Rhizaria ability to thrive in oceanic ecosystems, the mecha...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2015.00098

    authors: Balzano S,Corre E,Decelle J,Sierra R,Wincker P,Da Silva C,Poulain J,Pawlowski J,Not F

    更新日期:2015-03-17 00:00:00

  • Modeling the Inactivation of Intestinal Pathogenic Escherichia coli O157:H7 and Uropathogenic E. coli in Ground Chicken by High Pressure Processing and Thymol.

    abstract::Disease causing Escherichia coli commonly found in meat and poultry include intestinal pathogenic E. coli (iPEC) as well as extraintestinal types such as the Uropathogenic E. coli (UPEC). In this study we compared the resistance of iPEC (O157:H7) to UPEC in chicken meat using High Pressure Processing (HPP) in with (th...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2016.00920

    authors: Chien SY,Sheen S,Sommers CH,Sheen LY

    更新日期:2016-06-14 00:00:00

  • Two Major Clades of Bradyrhizobia Dominate Symbiotic Interactions with Pigeonpea in Fields of Côte d'Ivoire.

    abstract::In smallholder farms of Côte d'Ivoire, particularly in the northeast of the country, Cajanus cajan (pigeonpea) has become an important crop because of its multiple beneficial facets. Pigeonpea seeds provide food to make ends meet, are sold on local markets, and aerial parts serve as forage for animals. Since it fixes ...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2016.01793

    authors: Fossou RK,Ziegler D,Zézé A,Barja F,Perret X

    更新日期:2016-11-11 00:00:00