当前位置： SCI文献检索 > Frontiers in Microbiology期刊下所有文献 > Biophysical methods for the study of microbial surfaces.

Biophysical methods for the study of microbial surfaces.

Abstract：

:The challenge in studying the surface architecture of different microbial pathogens is to integrate the most current biochemical, spectroscopic, microscopic, and processing techniques. Individually these methods have insufficient sensitivity to reveal complex structures, such as branched, large, viscous polymers with a high structure hydration, size, and complexity. However, when used in combination biophysical techniques are our primary source of information for understanding polydisperse molecules and complex microbial surfaces. Biophysical methods seek to explain biological function in terms of the molecular structures and properties of specific molecules. The sizes of the molecules found in microbial surfaces vary greatly from small fatty acids and sugars to macromolecules like proteins, polysaccharides, and pigments, such as melanin. These molecules, which comprise the building blocks of living organisms, assemble into cells, tissues, and whole organisms by forming complex individual structures with dimensions from 10 to 10,000 nm and larger. Biophysics is directed to determining the structure of specific biological molecules and of the larger structures into which they assemble. Some of this effort involves developing new methods, adapting old methods and building new instruments for viewing these structures. The description of biophysical properties in an experimental model where, properties such as flexibility, hydrodynamic characteristics, and size can be precisely determined is of great relevance to study the affinity of the surfaces with biologically active and inert substrates and the interaction with host molecules. Furthermore, this knowledge could establish the abilities of different molecules and their structures to differentially activate cellular responses. Recent studies in the fungal pathogen Cryptococcus neoformans have demonstrated that the physical properties of its unique polysaccharide capsule correlate with the biological functions associated with the intact capsule and the components comprising the capsule. In this review, we describe the application of biophysical techniques to study and characterize this highly hydrated and fragile fungal surface structure.

journal_name

Front Microbiol

journal_title

Frontiers in microbiology

authors

Frases S,Viana NB,Casadevall A

doi

10.3389/fmicb.2011.00207

subject

Has Abstract

pub_date

2011-10-10 00:00:00

pages

207

issn

1664-302X

journal_volume

pub_type

杂志文章

在线工具

Biophysical methods for the study of microbial surfaces.