Continuous Adaptive Evolution of a Fast-Growing Corynebacterium glutamicum Strain Independent of Protocatechuate.

Abstract:

:Corynebacterium glutamicum is a commonly applied host for the industrial production of amino acids. While valued for its robustness, it is somewhat inferior to competing strains such as Escherichia coli because of the relatively low growth rate of 0.40 h-1 in synthetic, industrial media. Accordingly, adaptive laboratory evolution (ALE) experiments were performed in continuous cultivation mode to select for a growth-improved host. To ensure industrial attractiveness, this ALE study aimed at a reduction of dependency on costly growth-boosting additives such as protocatechuate (PCA) or complex media supplements. Consequently, double selection pressures were installed consisting of a steady increase in growth rate demands and a parallel reduction of complex medium fractions. Selection yielded C. glutamicum EVO5 achieving 0.54 h-1 and 1.03 gGlc gCDW-1 h-1 in minimal medium without abovementioned supplements. Sequencing revealed 10 prominent mutations, three of them in key regulator genes.

journal_name

Front Microbiol

authors

Graf M,Haas T,Müller F,Buchmann A,Harm-Bekbenbetova J,Freund A,Nieß A,Persicke M,Kalinowski J,Blombach B,Takors R

doi

10.3389/fmicb.2019.01648

subject

Has Abstract

pub_date

2019-08-06 00:00:00

pages

1648

issn

1664-302X

journal_volume

10

pub_type

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