Expression, Purification and Characterization of the Hepatitis E Virus Like-Particles in the Pichia pastoris.

Abstract:

:Hepatitis E virus (HEV) is associated with acute hepatitis disease, which may lead to chronic disease in immunocompromised individuals. The disease is particularly severe among pregnant women (20-30% mortality). The only licensed vaccine against HEV, which is available in China, is the Escherichia coli purified recombinant virus-like particles (VLPs) encompassing the 368-660 amino acids (aa) of the viral ORF2 protein. The viral capsid is formed by the ORF2 protein, which harbors three glycosylation sites. Baculo virus expression system has been employed to generate a glycosylated VLP, which encompasses 112-608aa of the ORF2 protein. Here, we sought to produce a recombinant VLP containing 112-608aa of the ORF2 protein in Pichia pastoris (P. pastoris) expression system. The cDNA sequence encoding 112-608aa of the ORF2 protein was fused with the α-mating factor secretion signal coding sequence (for release of the fusion protein to the culture medium) and cloned into the yeast vector pPICZα. Optimum expression of recombinant protein was obtained at 72 h induction in 1.5% methanol using inoculum density (A600) of 80 and at pH-3.0 of the culture medium. Identity of the purified protein was confirmed by mass spectrometry analysis. Further studies revealed the glycosylation pattern and VLP nature of the purified protein. Immunization of BALB/c mice with these VLPs induced potent immune response as evidenced by the high ORF2 specific IgG titer and augmented splenocyte proliferation in a dose dependent manner. 112-608aa ORF2 VLPs produced in P. pastoris appears to be a suitable candidate for development of diagnostic and prophylactic reagents against the hepatitis E.

journal_name

Front Microbiol

authors

Gupta J,Kaul S,Srivastava A,Kaushik N,Ghosh S,Sharma C,Batra G,Banerjee M,Shalimar,Nayak B,Ranjith-Kumar CT,Surjit M

doi

10.3389/fmicb.2020.00141

subject

Has Abstract

pub_date

2020-02-06 00:00:00

pages

141

issn

1664-302X

journal_volume

11

pub_type

杂志文章
  • Characterization of Unexplored Deadwood Mycobiome in Highly Diverse Subtropical Forests Using Culture-independent Molecular Technique.

    abstract::The deadwood mycobiome, also known as wood-inhabiting fungi (WIF), are among the key players in wood decomposition, having a large impact on nutrient cycling in forest soils. However, our knowledge of WIF richness and distribution patterns in different forest biomes is limited. Here, we used pyrotag sequencing of the ...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2017.00574

    authors: Purahong W,Pietsch KA,Lentendu G,Schöps R,Bruelheide H,Wirth C,Buscot F,Wubet T

    更新日期:2017-04-19 00:00:00

  • Resistance Breeding of Common Bean Shapes the Physiology of the Rhizosphere Microbiome.

    abstract::The taxonomically diverse rhizosphere microbiome contributes to plant nutrition, growth and health, including protection against soil-borne pathogens. We previously showed that breeding for Fusarium-resistance in common bean changed the rhizosphere microbiome composition and functioning. Here, we assessed the impact o...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2019.02252

    authors: Mendes LW,de Chaves MG,Fonseca MC,Mendes R,Raaijmakers JM,Tsai SM

    更新日期:2019-10-01 00:00:00

  • The Cacti Microbiome: Interplay between Habitat-Filtering and Host-Specificity.

    abstract::Cactaceae represents one of the most species-rich families of succulent plants native to arid and semi-arid ecosystems, yet the associations Cacti establish with microorganisms and the rules governing microbial community assembly remain poorly understood. We analyzed the composition, diversity, and factors influencing...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2016.00150

    authors: Fonseca-García C,Coleman-Derr D,Garrido E,Visel A,Tringe SG,Partida-Martínez LP

    更新日期:2016-02-12 00:00:00

  • Gymnemic Acids Inhibit Adhesive Nanofibrillar Mediated Streptococcus gordonii-Candida albicans Mono-Species and Dual-Species Biofilms.

    abstract::Dental caries and periodontitis are the most common oral disease of all age groups, affecting billions of people worldwide. These oral diseases are mostly associated with microbial biofilms in the oral cavity. Streptococcus gordonii, an early tooth colonizing bacterium and Candida albicans, an opportunistic pathogenic...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2019.02328

    authors: Veerapandian R,Vediyappan G

    更新日期:2019-10-11 00:00:00

  • Alkyl Protocatechuate-Loaded Nanostructured Lipid Systems as a Treatment Strategy for Paracoccidioides brasiliensis and Paracoccidioides lutzii In Vitro.

    abstract::Dodecyl protocatechuate (dodecyl) is a derivative of protocatechuic acid (3,4-dihydroxybenzoic acid) that possesses anti-oxidant and antifungal properties. Nanostructured lipid systems (NLS) can potentiate the action of many antifungal agents, reducing the required dose and side effects by improving their activity. Th...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2017.01048

    authors: Medina-Alarcón KP,Singulani JL,Voltan AR,Sardi JCO,Petrônio MS,Santos MB,Polaquini CR,Regasini LO,Bolzani VS,da Silva DHS,Chorilli M,Mendes-Giannini MJS,Fusco-Almeida AM

    更新日期:2017-06-12 00:00:00

  • Assessing Population Diversity of Brettanomyces Yeast Species and Identification of Strains for Brewing Applications.

    abstract::Brettanomyces yeasts have gained popularity in many sectors of the biotechnological industry, specifically in the field of beer production, but also in wine and ethanol production. Their unique properties enable Brettanomyces to outcompete conventional brewer's yeast in industrially relevant traits such as production ...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2020.00637

    authors: Colomer MS,Chailyan A,Fennessy RT,Olsson KF,Johnsen L,Solodovnikova N,Forster J

    更新日期:2020-04-09 00:00:00

  • Contribution of Acinetobacter-derived cephalosporinase-30 to sulbactam resistance in Acinetobacter baumannii.

    abstract::The sulbactam resistance rate in Acinetobacter baumannii has increased worldwide. Previous reports have shown that the β-lactamase bla TEM-1 confers resistance to sulbactam in A. baumannii. The purpose of this study was to examine whether other β-lactamases, including the Acinetobacter-derived cephalosporinase (ADC), ...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2015.00231

    authors: Kuo SC,Lee YT,Yang Lauderdale TL,Huang WC,Chuang MF,Chen CP,Su SC,Lee KR,Chen TL

    更新日期:2015-03-25 00:00:00

  • Antimicrobial Protection of Marsupial Pouch Young.

    abstract::Marsupials diverged from eutherian mammals about 148 million years ago and represent a unique lineage of mammals with distinctive morphological and reproductive characteristics. Marsupials have significantly shorter gestation periods than eutherians. Pregnancy typically ranges from 15 to 35 days, with young being born...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章,评审

    doi:10.3389/fmicb.2017.00354

    authors: Cheng Y,Belov K

    更新日期:2017-03-07 00:00:00

  • Composition of Total and Cell-Proliferating Bacterioplankton Community in Early Summer in the North Sea - Roseobacters Are the Most Active Component.

    abstract::Heterotrophic bacterioplankton communities play an important role in organic matter processing in the oceans worldwide. In order to investigate the significance of distinct phylogenetic bacterial groups it is not only important to assess their quantitative abundance but also their growth dynamics in relation to the en...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2017.01771

    authors: Bakenhus I,Dlugosch L,Billerbeck S,Giebel HA,Milke F,Simon M

    更新日期:2017-09-13 00:00:00

  • Identification of Novel Genes Mediating Survival of Salmonella on Low-Moisture Foods via Transposon Sequencing Analysis.

    abstract::Salmonella enterica is the leading foodborne pathogen associated with outbreaks involving low-moisture foods (LMFs). However, the genes involved in Salmonella's long-term survival on LMFs remain poorly characterized. In this study, in-shell pistachios were inoculated with Tn5-based mutant libraries of S. Enteritidis P...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2020.00726

    authors: Jayeola V,McClelland M,Porwollik S,Chu W,Farber J,Kathariou S

    更新日期:2020-05-15 00:00:00

  • An RNA Virome Associated to the Golden Orb-Weaver Spider Nephila clavipes.

    abstract::The golden orb-weaver spider Nephila clavipes, known for its sexual size dimorphism, is abundant and widespread in the New World. The first annotated genome of orb-weaver spiders, exploring N. clavipes, has recently been reported. The study, focused primarily on the diversity of silk specific genes, shed light into th...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2017.02097

    authors: Debat HJ

    更新日期:2017-10-25 00:00:00

  • Diversity and Temporal Dynamics of the Epiphytic Bacterial Communities Associated with the Canopy-Forming Seaweed Cystoseira compressa (Esper) Gerloff and Nizamuddin.

    abstract::Canopy-forming seaweed species of the genus Cystoseira form diverse and productive habitats along temperate rocky coasts of the Mediterranean Sea. Despite numerous studies on the rich macrofauna and flora associated with Cystoseira spp., there is little knowledge about the epiphytic bacteria. We analyzed bacterial pop...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2016.00476

    authors: Mancuso FP,D'Hondt S,Willems A,Airoldi L,De Clerck O

    更新日期:2016-04-08 00:00:00

  • Inhibitory Effect of Volatiles Emitted From Alcaligenes faecalis N1-4 on Aspergillus flavus and Aflatoxins in Storage.

    abstract::Controlling aflatoxigenic Aspergillus flavus and aflatoxins (AFs) in grains and food during storage is a great challenge to humans worldwide. Alcaligenes faecalis N1-4 isolated from tea rhizosphere soil can produce abundant antifungal volatiles, and greatly inhibited the growth of A. flavus in un-contacted face-to-fac...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2019.01419

    authors: Gong AD,Wu NN,Kong XW,Zhang YM,Hu MJ,Gong SJ,Dong FY,Wang JH,Zhao ZY,Liao YC

    更新日期:2019-06-25 00:00:00

  • Modeling Multispecies Gene Flow Dynamics Reveals the Unique Roles of Different Horizontal Gene Transfer Mechanisms.

    abstract::Horizontal gene transfer within diverse bacterial populations occurs through multiple mechanisms of exchange. The most established routes of gene transfer, transduction, transformation, and conjugation, have been characterized in detail, revealing the advantages and limitations of each mechanism. More recently, inters...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2018.02978

    authors: Nazarian P,Tran F,Boedicker JQ

    更新日期:2018-12-04 00:00:00

  • Mass Spectrometry Guided Discovery and Design of Novel Asperphenamate Analogs From Penicillium astrolabium Reveals an Extraordinary NRPS Flexibility.

    abstract::Asperphenamate is a small peptide natural product that has gained much interest due to its antitumor activity. In the recent years numerous bioactive synthetic asperphenamate analogs have been reported, whereas only a handful of natural analogs either of microbial or plant origin has been discovered. Herein we describ...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2020.618730

    authors: Subko K,Wang X,Nielsen FH,Isbrandt T,Gotfredsen CH,Ramos MC,Mackenzie T,Vicente F,Genilloud O,Frisvad JC,Larsen TO

    更新日期:2021-01-15 00:00:00

  • Diversity and Expression of Bacterial Metacaspases in an Aquatic Ecosystem.

    abstract::Metacaspases are distant homologs of metazoan caspase proteases, implicated in stress response, and programmed cell death (PCD) in bacteria and phytoplankton. While the few previous studies on metacaspases have relied on cultured organisms and sequenced genomes, no studies have focused on metacaspases in a natural set...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2016.01043

    authors: Asplund-Samuelsson J,Sundh J,Dupont CL,Allen AE,McCrow JP,Celepli NA,Bergman B,Ininbergs K,Ekman M

    更新日期:2016-07-06 00:00:00

  • No Evidence That Homologs of Key Circadian Clock Genes Direct Circadian Programs of Development or mRNA Abundance in Verticillium dahliae.

    abstract::Many organisms harbor circadian clocks that promote their adaptation to the rhythmic environment. While a broad knowledge of the molecular mechanism of circadian clocks has been gained through the fungal model Neurospora crassa, little is known about circadian clocks in other fungi. N. crassa belongs to the same class...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2020.01977

    authors: Cascant-Lopez E,Crosthwaite SK,Johnson LJ,Harrison RJ

    更新日期:2020-08-28 00:00:00

  • Respiratory Commensal Bacteria Corynebacterium pseudodiphtheriticum Improves Resistance of Infant Mice to Respiratory Syncytial Virus and Streptococcus pneumoniae Superinfection.

    abstract::Corynebacterium pseudodiphtheriticum is a Gram-positive bacterium found as a member of the normal microbiota of the upper respiratory tract. It was suggested that C. pseudodiphtheriticum may be potentially used as a next-generation probiotic for nasal application, although no deep studies were performed in this regard...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2017.01613

    authors: Kanmani P,Clua P,Vizoso-Pinto MG,Rodriguez C,Alvarez S,Melnikov V,Takahashi H,Kitazawa H,Villena J

    更新日期:2017-08-23 00:00:00

  • Genomic Characteristics Distinguish Geographically Distributed Dehalococcoidia.

    abstract::Dehalococcoidia (Dia) class microorganisms are frequently found in various pristine and contaminated environments. Metagenome-assembled genomes (MAGs) and single-cell amplified genomes (SAGs) studies have substantially improved the understanding of Dia microbial ecology and evolution; however, an updated thorough inve...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2020.546063

    authors: Yang Y,Zhang Y,Cápiro NL,Yan J

    更新日期:2020-09-08 00:00:00

  • Evaluation of Probiotic Properties and Safety of Enterococcus faecium Isolated From Artisanal Tunisian Meat "Dried Ossban".

    abstract::Enterococcus faecium strains were isolated from an original biotope, artisanal dried Tunisian meat "Dried Ossban," and evaluated for safety and capacity as probiotics. Gram-positive, catalase negative, and bacteriocin-producing bacteria were screened using selective microbiological media. All isolates were identified ...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2018.01685

    authors: Zommiti M,Cambronel M,Maillot O,Barreau M,Sebei K,Feuilloley M,Ferchichi M,Connil N

    更新日期:2018-08-06 00:00:00

  • Diversity of Pelagic and Benthic Bacterial Assemblages in the Western Pacific Ocean.

    abstract::Despite numerous studies on marine prokaryotes, the vertical distribution patterns of bacterial community, either on the taxonomic composition or the functional structure, remains relatively unexplored. Using HiSeq-derived 16S rRNA data, the depth-related distribution patterns of taxonomic diversity and functional str...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2020.01730

    authors: Wang M,Ma Y,Feng C,Cai L,Li W

    更新日期:2020-09-10 00:00:00

  • Origin, diversity, and maturation of human antiviral antibodies analyzed by high-throughput sequencing.

    abstract::Our understanding of how antibodies are generated and function could help develop effective vaccines and antibody-based therapeutics against viruses such as HIV-1, SARS coronavirus (SARS CoV), and Hendra and Nipah viruses (henipaviruses). Although broadly neutralizing antibodies (bnAbs) against the HIV-1 were observed...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2012.00277

    authors: Prabakaran P,Zhu Z,Chen W,Gong R,Feng Y,Streaker E,Dimitrov DS

    更新日期:2012-08-02 00:00:00

  • Anti-parasitic Peptides from Arthropods and their Application in Drug Therapy.

    abstract::Africa, Asia, and Latin America are regions highly affected by endemic diseases, such as Leishmaniasis, Malaria, and Chagas' disease. They are responsible for the death of 1000s of patients every year, as there is not yet a cure for them and the drugs used are inefficient against the pathogenic parasites. During the l...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章,评审

    doi:10.3389/fmicb.2016.00091

    authors: Lacerda AF,Pelegrini PB,de Oliveira DM,Vasconcelos ÉA,Grossi-de-Sá MF

    更新日期:2016-02-05 00:00:00

  • BALOs Improved Gut Microbiota Health in Postlarval Shrimp (Litopenaeus vannamei) After Being Subjected to Salinity Reduction Treatment.

    abstract::White shrimp, Litopenaeus vannamei, is a widely farmed species. In China, shrimp postlarvae (PL) are frequently subjected to salinity reduction treatment to meet end growers' needs. However, although this treatment effectively reduces vibrio counts, its impact on gut microbiota health is still unknown. In this study, ...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2020.01296

    authors: Cao Q,Najnine F,Han H,Wu B,Cai J

    更新日期:2020-06-30 00:00:00

  • Identification of Genes Involved in Biogenesis of Outer Membrane Vesicles (OMVs) in Salmonella enterica Serovar Typhi.

    abstract::Outer membrane vesicles (OMVs) are nano-sized proteoliposomes discharged from the cell envelope of Gram-negative bacteria. OMVs normally contain toxins, enzymes and other factors, and are used as vehicles in a process that has been considered a generalized, evolutionarily conserved delivery system among bacteria. Furt...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2019.00104

    authors: Nevermann J,Silva A,Otero C,Oyarzún DP,Barrera B,Gil F,Calderón IL,Fuentes JA

    更新日期:2019-02-04 00:00:00

  • Prevalence, Molecular Characterization, and Antibiotic Susceptibility of Cronobacter sakazakii Isolates from Powdered Infant Formula Collected from Chinese Retail Markets.

    abstract::Cronobacter sakazakii is an opportunistic pathogen that causes severe infections in neonates and infants through contaminated powdered infant formula (PIF). Therefore, the aim of this study was a large-scale study on determine the prevalence, molecular characterization and antibiotic susceptibility of C. sakazakii iso...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2017.02026

    authors: Fei P,Jiang Y,Jiang Y,Yuan X,Yang T,Chen J,Wang Z,Kang H,Forsythe SJ

    更新日期:2017-10-17 00:00:00

  • Pseudomonas aeruginosa Quorum-Sensing and Type VI Secretion System Can Direct Interspecific Coexistence During Evolution.

    abstract::It is reported that a wide range of bacterial infections are polymicrobial, and the members in a local microcommunity can influence the growth of neighbors through physical and chemical interactions. Pseudomonas aeruginosa is an important opportunistic pathogen that normally causes a variety of acute and chronic infec...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2018.02287

    authors: Zhao K,Du L,Lin J,Yuan Y,Wang X,Yue B,Wang X,Guo Y,Chu Y,Zhou Y

    更新日期:2018-10-11 00:00:00

  • Subgingival microbiota in health compared to periodontitis and the influence of smoking.

    abstract::The etiology of periodontitis has traditionally been associated to a consortium of three bacterial species-the so-called "red-complex" of periodontal disease-which has been the target for most diagnostic and therapeutic strategies. However, other species have also been found to correlate with disease severity. In addi...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2015.00119

    authors: Camelo-Castillo AJ,Mira A,Pico A,Nibali L,Henderson B,Donos N,Tomás I

    更新日期:2015-02-24 00:00:00

  • Probiotic Lactobacillus rhamnosus GG Promotes Mouse Gut Microbiota Diversity and T Cell Differentiation.

    abstract::Lactic acid bacteria (LAB) are the primary genera of the intestinal flora and have many probiotic functions. In the present study, Lactobacillus rhamnosus GG (LGG) ATCC 53103 was used to treat BALB/c mice. After LGG intervention, both low and high LGG doses were shown to improve the observed OTU, Chao1, ACE, and Shann...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2020.607735

    authors: Shi CW,Cheng MY,Yang X,Lu YY,Yin HD,Zeng Y,Wang RY,Jiang YL,Yang WT,Wang JZ,Zhao DD,Huang HB,Ye LP,Cao X,Yang GL,Wang CF

    更新日期:2020-12-17 00:00:00

  • Differential NtcA Responsiveness to 2-Oxoglutarate Underlies the Diversity of C/N Balance Regulation in Prochlorococcus.

    abstract::Previous studies showed differences in the regulatory response to C/N balance in Prochlorococcus with respect to other cyanobacteria, but no information was available about its causes, or the ecological advantages conferred to thrive in oligotrophic environments. We addressed the changes in key enzymes (glutamine synt...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2017.02641

    authors: Domínguez-Martín MA,López-Lozano A,Clavería-Gimeno R,Velázquez-Campoy A,Seidel G,Burkovski A,Díez J,García-Fernández JM

    更新日期:2018-01-09 00:00:00