Characterization of a Novel Tanay Virus Isolated From Anopheles sinensis Mosquitoes in Yunnan, China.

Abstract:

:Globally, mosquitoes are known to be competent vectors to various arboviruses that cause serious and debilitating diseases to humans and animals. Conversely, mosquitoes harbor a wide array of insect specific viruses (ISVs) that are generally neglected. Extensive characterization of these ISVs is important in understanding their persistence infection effect on host behavior and arbovirus transmission. Herein, we report first time isolation of Tanay virus (TANAV) isolate YN15_103_01 in Anopheles sinensis mosquitoes from Yunnan Province, China. Phylogenetically, the isolate's nucleotide identity had more than 14.47% variance compared to previous TANAV isolates, and it clustered into an independent branch within the genus Sandewavirus in the newly proposed taxon Negevirus. TANAV growth and high titers was attained in Aag2 cells (107 PFU/mL) but with no CPE observed up to 7 days.p.i. compared to C6/36 cells that exhibited extensive CPE at 48 h.p.i. with titers of 107 PFU/mL. Contrarywise, the viral isolate did not replicate in vertebrate cell lines. Electron microscopy analyses showed that its final maturation process takes place in the cell cytoplasm. Notably, the predicted viral proteins were verified to be corresponding to the obtained SDS-PAGE protein bands. Our findings advance forth new and vital knowledge important in understanding insect specific viruses, especially TANAV.

journal_name

Front Microbiol

authors

Zhao L,Mwaliko C,Atoni E,Wang Y,Zhang Y,Zhan J,Hu X,Xia H,Yuan Z

doi

10.3389/fmicb.2019.01963

subject

Has Abstract

pub_date

2019-08-22 00:00:00

pages

1963

issn

1664-302X

journal_volume

10

pub_type

杂志文章
  • Fungal ITS1 Deep-Sequencing Strategies to Reconstruct the Composition of a 26-Species Community and Evaluation of the Gut Mycobiota of Healthy Japanese Individuals.

    abstract::The study of mycobiota remains relatively unexplored due to the lack of sufficient available reference strains and databases compared to those of bacterial microbiome studies. Deep sequencing of Internal Transcribed Spacer (ITS) regions is the de facto standard for fungal diversity analysis. However, results are often...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2017.00238

    authors: Motooka D,Fujimoto K,Tanaka R,Yaguchi T,Gotoh K,Maeda Y,Furuta Y,Kurakawa T,Goto N,Yasunaga T,Narazaki M,Kumanogoh A,Horii T,Iida T,Takeda K,Nakamura S

    更新日期:2017-02-15 00:00:00

  • Binding of Campylobacter jejuni FliW Adjacent to the CsrA RNA-Binding Pockets Modulates CsrA Regulatory Activity.

    abstract::Campylobacter jejuni CsrA is an mRNA-binding, post-transcriptional regulator that controls many metabolic- and virulence-related characteristics of this important pathogen. In contrast to E. coli CsrA, whose activity is modulated by binding to small non-coding RNAs (sRNAs), C. jejuni CsrA activity is controlled by bin...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2020.531596

    authors: Bogacz M,El Abbar FM,Cox CA,Li J,Fiedler JS,Tran LKH,Tran PMH,Daugherty CL,Blake KH,Wang Z,Azadi P,Thompson SA

    更新日期:2021-01-11 00:00:00

  • Adaptation to Bleaching: Are Thermotolerant Symbiodiniaceae Strains More Successful Than Other Strains Under Elevated Temperatures in a Model Symbiotic Cnidarian?

    abstract::The ability of some symbiotic cnidarians to resist and better withstand stress factors that cause bleaching is a trait that is receiving increased attention. The adaptive bleaching hypothesis postulates that cnidarians that can form a stable symbiosis with thermotolerant Symbiodiniaceae strains may cope better with in...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2020.00822

    authors: Newkirk CR,Frazer TK,Martindale MQ,Schnitzler CE

    更新日期:2020-05-05 00:00:00

  • Resistance of Animal Strains of Pseudomonas aeruginosa to Carbapenems.

    abstract::Carbapenems are major antibiotics reserved to human medicine. This study aimed to investigate the mechanisms of carbapenem resistance of a selection of Pseudomonas aeruginosa veterinary strains from the French network Resapath. Thirty (5.7%) imipenem and/or meropenem non-susceptible P. aeruginosa of canine (n = 24), f...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2017.01847

    authors: Haenni M,Bour M,Châtre P,Madec JY,Plésiat P,Jeannot K

    更新日期:2017-09-29 00:00:00

  • Atypical Porcine Pestivirus as a Novel Type of Pestivirus in Pigs in China.

    abstract::Pestiviruses are highly variable RNA viruses. A growing number of novel pestiviruses has been discovered in domestic and wild species in the last two decades. Recently, a novel atypical porcine pestivirus (APPV) linked with the development of congenital tremor (CT) in neonatal pigs was described in Europe and the Amer...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2017.00862

    authors: Yuan J,Han Z,Li J,Huang Y,Yang J,Ding H,Zhang J,Zhu M,Zhang Y,Liao J,Zhao M,Chen J

    更新日期:2017-05-11 00:00:00

  • Alpine Snow Algae Microbiome Diversity in the Coast Range of British Columbia.

    abstract::Snow algae blooms contain bacteria, fungi, and other microscopic organisms. We surveyed 55 alpine snow algae blooms, collecting a total of 68 samples, from 12 mountains in the Coast Range of British Columbia, Canada. We used microscopy and rDNA metabarcoding to document biodiversity and query species and taxonomic ass...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2020.01721

    authors: Yakimovich KM,Engstrom CB,Quarmby LM

    更新日期:2020-07-28 00:00:00

  • Rapid Sequencing of Multiple RNA Viruses in Their Native Form.

    abstract::Long-read nanopore sequencing by a MinION device offers the unique possibility to directly sequence native RNA. We combined an enzymatic poly-A tailing reaction with the native RNA sequencing to (i) sequence complex population of single-stranded (ss)RNA viruses in parallel, (ii) detect genome, subgenomic mRNA/mRNA sim...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2019.00260

    authors: Wongsurawat T,Jenjaroenpun P,Taylor MK,Lee J,Tolardo AL,Parvathareddy J,Kandel S,Wadley TD,Kaewnapan B,Athipanyasilp N,Skidmore A,Chung D,Chaimayo C,Whitt M,Kantakamalakul W,Sutthent R,Horthongkham N,Ussery DW,Jonsson

    更新日期:2019-02-25 00:00:00

  • Serologic Evaluation of Healthcare Workers Caring for COVID-19 Patients in the Republic of Korea.

    abstract::The safety of healthcare workers (HCWs) against severe acute respiratory syndrome virus 2 (SARS-CoV-2) transmission is an important aspect of managing the coronavirus disease 2019 (COVID-19) pandemic. In the South Korea, highly stringent infection prevention and control (IPC) guidelines are implemented, and reports of...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2020.587613

    authors: Ko JH,Lee JY,Kim HA,Kang SJ,Baek JY,Park SJ,Hyun M,Jo IJ,Chung CR,Kim YJ,Kang ES,Choi YK,Chang HH,Jung SI,Peck KR

    更新日期:2020-11-20 00:00:00

  • Use of Rhizobacteria and Mycorrhizae Consortium in the Open Field as a Strategy for Improving Crop Nutrition, Productivity and Soil Fertility.

    abstract::Plant growth promoting rhizobacteria (PGPR) and arbuscular mycorrhizal fungi (AMF) are known for their beneficial effects. In recent years, more attention has been paid to their use as biofertilizers to reduce the use of chemical fertilizers causing significant damage to the environment. To have high plant yields, bio...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2019.01106

    authors: Raklami A,Bechtaoui N,Tahiri AI,Anli M,Meddich A,Oufdou K

    更新日期:2019-05-21 00:00:00

  • Bacillus amyloliquefaciens Ameliorates Dextran Sulfate Sodium-Induced Colitis by Improving Gut Microbial Dysbiosis in Mice Model.

    abstract::Several Bacillus strains exert beneficial effects on the maintenance of intestinal homeostasis and host health. However, whether Bacillus amyloliquefaciens (BA) can improve gut microbial dysbiosis and ameliorate colitis is unknown. Therefore, we conducted the present study to investigate the effects of BA administrati...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2018.03260

    authors: Cao G,Wang K,Li Z,Tao F,Xu Y,Lan J,Chen G,Yang C

    更新日期:2019-01-08 00:00:00

  • From Petri Dish to Patient: Bioavailability Estimation and Mechanism of Action for Antimicrobial and Immunomodulatory Natural Products.

    abstract::The new era of multidrug resistance of pathogens against frontline antibiotics has compromised the immense therapeutic gains of the 'golden age,' stimulating a resurgence in antimicrobial research focused on antimicrobial and immunomodulatory components of botanical, fungal or microbial origin. While much valuable inf...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章,评审

    doi:10.3389/fmicb.2019.02470

    authors: Sadgrove NJ,Jones GL

    更新日期:2019-10-31 00:00:00

  • Composition of Total and Cell-Proliferating Bacterioplankton Community in Early Summer in the North Sea - Roseobacters Are the Most Active Component.

    abstract::Heterotrophic bacterioplankton communities play an important role in organic matter processing in the oceans worldwide. In order to investigate the significance of distinct phylogenetic bacterial groups it is not only important to assess their quantitative abundance but also their growth dynamics in relation to the en...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2017.01771

    authors: Bakenhus I,Dlugosch L,Billerbeck S,Giebel HA,Milke F,Simon M

    更新日期:2017-09-13 00:00:00

  • Diatom-Bacteria Interactions Modulate the Composition and Productivity of Benthic Diatom Biofilms.

    abstract::Benthic diatoms are dominant primary producers in intertidal mudflats and constitute a major source of organic carbon to consumers and decomposers residing within these ecosystems. They typically form biofilms whose species richness, community composition and productivity can vary in response to environmental drivers ...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2019.01255

    authors: Koedooder C,Stock W,Willems A,Mangelinckx S,De Troch M,Vyverman W,Sabbe K

    更新日期:2019-06-05 00:00:00

  • Challenging Oil Bioremediation at Deep-Sea Hydrostatic Pressure.

    abstract::The Deepwater Horizon accident has brought oil contamination of deep-sea environments to worldwide attention. The risk for new deep-sea spills is not expected to decrease in the future, as political pressure mounts to access deep-water fossil reserves, and poorly tested technologies are used to access oil. This also a...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章,评审

    doi:10.3389/fmicb.2016.01203

    authors: Scoma A,Yakimov MM,Boon N

    更新日期:2016-08-03 00:00:00

  • Prioritized Expression of BDH2 under Bulk Translational Repression and Its Contribution to Tolerance to Severe Vanillin Stress in Saccharomyces cerevisiae.

    abstract::Vanillin is a potent fermentation inhibitor derived from the lignocellulosic biomass in biofuel production, and high concentrations of vanillin result in the pronounced repression of bulk translation in Saccharomyces cerevisiae. Studies on genes that are efficiently translated even in the presence of high concentratio...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2016.01059

    authors: Ishida Y,Nguyen TT,Kitajima S,Izawa S

    更新日期:2016-07-06 00:00:00

  • Anti-microfouling Activity of Glycomyces sediminimaris UTMC 2460 on Dominant Fouling Bacteria of Iran Marine Habitats.

    abstract::Discovery of environmentally safe anti-fouling agent is currently of considerable interest, due to the continuous impact of biofoulers on the marine habitats and the adverse effects of biocides on the environment. This study reports the anti-adhesion effect of marine living Actinobacteria against fouling strains isola...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2018.03148

    authors: Heidarian S,Mohammadipanah F,Maghsoudlou A,Dashti Y,Challis GL

    更新日期:2019-01-09 00:00:00

  • Characterization of a Fusarium graminearum Salicylate Hydroxylase.

    abstract::Salicylic acid (SA) plays an important role in regulating plant defense responses against pathogens. However, pathogens have evolved ways to manipulate plant SA-mediated defense signaling. Fusarium graminearum causes Fusarium head blight (FHB) and reduces crop yields and quality by producing various mycotoxins. In thi...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2018.03219

    authors: Hao G,Naumann TA,Vaughan MM,McCormick S,Usgaard T,Kelly A,Ward TJ

    更新日期:2019-01-08 00:00:00

  • Determining the Distribution of Fluorescent Organic Matter in the Indian Ocean Using in situ Fluorometry.

    abstract::In order to determine the dynamics of marine fluorescent organic matter (FOM) using high-resolution spatial data, in situ fluorometers have been used in the open ocean. In this study, we measured FOM during the Global Ocean Ship-based Hydrographic Investigations Program (GO-SHIP) expedition from early December 2019 to...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2020.589262

    authors: Shigemitsu M,Uchida H,Yokokawa T,Arulananthan K,Murata A

    更新日期:2020-12-23 00:00:00

  • Fonsecaea pedrosoi Sclerotic Cells: Secretion of Aspartic-Type Peptidase and Susceptibility to Peptidase Inhibitors.

    abstract::Fonsecaea pedrosoi is a dematiaceous fungus and the main causative agent of chromoblastomycosis that is a chronic disease usually affecting the human skin and subcutaneous tissues, which causes deformations and incapacities, being frequently refractory to available therapies. A typical globe-shaped, multiseptated and ...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2018.01383

    authors: Palmeira VF,Goulart FRV,Granato MQ,Alviano DS,Alviano CS,Kneipp LF,Santos ALS

    更新日期:2018-06-29 00:00:00

  • Inhibitory Effect of Volatiles Emitted From Alcaligenes faecalis N1-4 on Aspergillus flavus and Aflatoxins in Storage.

    abstract::Controlling aflatoxigenic Aspergillus flavus and aflatoxins (AFs) in grains and food during storage is a great challenge to humans worldwide. Alcaligenes faecalis N1-4 isolated from tea rhizosphere soil can produce abundant antifungal volatiles, and greatly inhibited the growth of A. flavus in un-contacted face-to-fac...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2019.01419

    authors: Gong AD,Wu NN,Kong XW,Zhang YM,Hu MJ,Gong SJ,Dong FY,Wang JH,Zhao ZY,Liao YC

    更新日期:2019-06-25 00:00:00

  • Modeling Multispecies Gene Flow Dynamics Reveals the Unique Roles of Different Horizontal Gene Transfer Mechanisms.

    abstract::Horizontal gene transfer within diverse bacterial populations occurs through multiple mechanisms of exchange. The most established routes of gene transfer, transduction, transformation, and conjugation, have been characterized in detail, revealing the advantages and limitations of each mechanism. More recently, inters...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2018.02978

    authors: Nazarian P,Tran F,Boedicker JQ

    更新日期:2018-12-04 00:00:00

  • Threonine Phosphorylation Fine-Tunes the Regulatory Activity of Histone-Like Nucleoid Structuring Protein in Salmonella Transcription.

    abstract::Histone-like nucleoid structuring protein (H-NS) in enterobacteria plays an important role in facilitating chromosome organization and functions as a crucial transcriptional regulator for global gene regulation. Here, we presented an observation that H-NS of Salmonella enterica serovar Typhimurium could undergo protei...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2019.01515

    authors: Hu L,Kong W,Yang D,Han Q,Guo L,Shi Y

    更新日期:2019-07-03 00:00:00

  • Ascorbic acid-dependent gene expression in Streptococcus pneumoniae and the activator function of the transcriptional regulator UlaR2.

    abstract::In this study, we have explored the impact of ascorbic acid on the transcriptome of Streptococcus pneumoniae D39. The expression of several genes and operons, including the ula operon (which has been previously shown to be involved in ascorbic acid utilization), the AdcR regulon (which has been previously shown to be ...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2015.00072

    authors: Afzal M,Shafeeq S,Kuipers OP

    更新日期:2015-02-11 00:00:00

  • In-Depth Characterization and Functional Analysis of Clonal Variants in a Mycobacterium tuberculosis Strain Prone to Microevolution.

    abstract::The role of clonal complexity has gradually been accepted in infection by Mycobacterium tuberculosis (MTB), although analyses of this issue are limited. We performed an in-depth study of a case of recurrent MTB infection by integrating genotyping, whole genome sequencing, analysis of gene expression and infectivity in...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2017.00694

    authors: Navarro Y,Pérez-Lago L,Herranz M,Sierra O,Comas I,Sicilia J,Bouza E,García de Viedma D

    更新日期:2017-04-24 00:00:00

  • A Novel MSCRAMM Subfamily in Coagulase Negative Staphylococcal Species.

    abstract::Coagulase negative staphylococci (CoNS) are important opportunistic pathogens. Staphylococcus epidermidis, a coagulase negative staphylococcus, is the third leading cause of nosocomial infections in the US. Surface proteins like Microbial Surface Components Recognizing Adhesive Matrix Molecules (MSCRAMMs) are major vi...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2016.00540

    authors: Arora S,Uhlemann AC,Lowy FD,Hook M

    更新日期:2016-04-29 00:00:00

  • Azathioprine, Mercaptopurine, and 5-Aminosalicylic Acid Affect the Growth of IBD-Associated Campylobacter Species and Other Enteric Microbes.

    abstract::Campylobacter concisus is a bacterium that is associated with inflammatory bowel disease (IBD). Immunosuppressive drugs including azathioprine (AZA) and mercaptopurine (MP), and anti-inflammatory drug such as 5-aminosalicylic acid (5-ASA) are commonly used to treat patients with IBD. This study aimed to examine the ef...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2017.00527

    authors: Liu F,Ma R,Riordan SM,Grimm MC,Liu L,Wang Y,Zhang L

    更新日期:2017-03-29 00:00:00

  • Marine sediments microbes capable of electrode oxidation as a surrogate for lithotrophic insoluble substrate metabolism.

    abstract::Little is known about the importance and/or mechanisms of biological mineral oxidation in sediments, partially due to the difficulties associated with culturing mineral-oxidizing microbes. We demonstrate that electrochemical enrichment is a feasible approach for isolation of microbes capable of gaining electrons from ...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2014.00784

    authors: Rowe AR,Chellamuthu P,Lam B,Okamoto A,Nealson KH

    更新日期:2015-01-14 00:00:00

  • The Influence of Viable Cells and Cell-Free Extracts of Lactobacillus casei on Volatile Compounds and Polyphenolic Profile of Elderberry Juice.

    abstract::In this study, four strains of Lactobacillus casei, as viable cells or cell-free extracts (CFE), were added to elderberry juice in order to evaluate their effect on phenolic and aromatic profile. Two of them were able to grow in juice while the others showed zero-growth. The same strains were lysed and added as extrac...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2018.02784

    authors: Ricci A,Levante A,Cirlini M,Calani L,Bernini V,Del Rio D,Galaverna G,Neviani E,Lazzi C

    更新日期:2018-11-20 00:00:00

  • Cell Wall Structure of Coccoid Green Algae as an Important Trade-Off Between Biotic Interference Mechanisms and Multidimensional Cell Growth.

    abstract::Coccoid green algae can be divided in two groups based on their cell wall structure. One group has a highly chemical resistant cell wall (HR-cell wall) containing algaenan. The other group is more susceptible to chemicals (LR-cell wall - Low resistant cell wall). Algaenan is considered as important molecule to explain...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2018.00719

    authors: Dunker S,Wilhelm C

    更新日期:2018-04-13 00:00:00

  • From Genes to Ecosystems in Microbiology: Modeling Approaches and the Importance of Individuality.

    abstract::Models are important tools in microbial ecology. They can be used to advance understanding by helping to interpret observations and test hypotheses, and to predict the effects of ecosystem management actions or a different climate. Over the past decades, biological knowledge and ecosystem observations have advanced to...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章,评审

    doi:10.3389/fmicb.2017.02299

    authors: Kreft JU,Plugge CM,Prats C,Leveau JHJ,Zhang W,Hellweger FL

    更新日期:2017-11-27 00:00:00