Serologic Evaluation of Healthcare Workers Caring for COVID-19 Patients in the Republic of Korea.

Abstract:

:The safety of healthcare workers (HCWs) against severe acute respiratory syndrome virus 2 (SARS-CoV-2) transmission is an important aspect of managing the coronavirus disease 2019 (COVID-19) pandemic. In the South Korea, highly stringent infection prevention and control (IPC) guidelines are implemented, and reports of healthcare-associated SARS-CoV-2 transmission among HCWs are limited. However, subclinical infections may have been missed by the current symptom-based screening strategy. To evaluate the risk of undetected SARS-CoV-2 transmissions from COVID-19 patients to HCWs, we conducted a multicenter seroprevalence study after the first surge of the COVID-19 outbreak. A total of 432 HCWs were evaluated, comprising 309 HCWs designated to laboratory-confirmed COVID-19 patient care and 123 non-designated HCWs. Designated HCWs wore personal protective equipment including an N95 respirator, eye protection, hooded overalls, shoe covers, and inner and outer gloves. Use of a powered air-purifying respirator was recommended for aerosol-generating procedures or long-duration care activities. A high-sensitivity (99.1%) fluorescence immunoassay immunoglobulin G (IgG) kit was used as the initial screening test, and two enzyme-linked immunosorbent assay kits for total and IgG antibodies were used to confirm the test results. A microneutralization test was additionally performed to evaluate the neutralizing activity of positive specimens. Among the evaluated HCWs, none of the non-designated HCWs had a positive result, while one of the HCWs designated for COVID-19 patient care (1/309, 0.3%) was seropositive for SARS-CoV-2 with confirmed neutralizing activity (1:40). This finding suggests that subclinical seroconversion may occur among HCWs caring for COVID-19 patients, although the risk is low under strict IPC guidance.

journal_name

Front Microbiol

authors

Ko JH,Lee JY,Kim HA,Kang SJ,Baek JY,Park SJ,Hyun M,Jo IJ,Chung CR,Kim YJ,Kang ES,Choi YK,Chang HH,Jung SI,Peck KR

doi

10.3389/fmicb.2020.587613

subject

Has Abstract

pub_date

2020-11-20 00:00:00

pages

587613

issn

1664-302X

journal_volume

11

pub_type

杂志文章
  • Replicon-Based Typing of IncI-Complex Plasmids, and Comparative Genomics Analysis of IncIγ/K1 Plasmids.

    abstract::IncI-complex plasmids can be divided into seven subgroups IncI1, IncI2, IncIγ, IncB/O, IncK1, IncK2, and IncZ. In this study, a replicon-based scheme was proposed for typing IncI-complex plasmids into four separately clustering subgroups IncI2, IncI1/B/O, IncIγ/K1 and IncK2/Z, the last three of which were combined fro...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2019.00048

    authors: Zhang D,Zhao Y,Feng J,Hu L,Jiang X,Zhan Z,Yang H,Yang W,Gao B,Wang J,Li J,Yin Z,Zhou D

    更新日期:2019-01-29 00:00:00

  • Association of TREM-1, IL-1β, IL-33/ST2, and TLR Expressions With the Pathogenesis of Ocular Toxoplasmosis in Mouse Models on Different Genetic Backgrounds.

    abstract::Ocular toxoplasmosis (OT) is one of the most common causes of posterior uveitis. The signaling of triggering receptor expressed on myeloid cells (TREM)-1 amplifies inflammation, whereas TREM-2 signaling is anti-inflammatory. IL-1β is a major driver of inflammation during infection. Toll-like receptors (TLRs) play impo...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2019.02264

    authors: Zhang Y,He J,Zheng H,Huang S,Lu F

    更新日期:2019-10-09 00:00:00

  • Estimating HIV-1 Genetic Diversity in Brazil Through Next-Generation Sequencing.

    abstract::Approximately 36.7 million people were living with the human immunodeficiency virus (HIV) at the end of 2016 according to UNAIDS, representing a global prevalence rate of 0.8%. In Brazil, an HIV prevalence of 0.24% has been estimated, which represents approximately 830,000 individuals living with the virus. As a touri...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2019.00749

    authors: Alves BM,Siqueira JD,Prellwitz IM,Botelho OM,Da Hora VP,Sanabani S,Recordon-Pinson P,Fleury H,Soares EA,Soares MA

    更新日期:2019-04-09 00:00:00

  • Probiotic Supplements: Hope or Hype?

    abstract::Probiotic bacteria have been associated with various health benefits and included in overwhelming number of foods. Today, probiotic supplements are consumed with increasing regularity and record a rapidly growing economic value. With billions of heterogeneous populations of probiotics per serving, probiotic supplement...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2020.00160

    authors: Wang Y,Jiang Y,Deng Y,Yi C,Wang Y,Ding M,Liu J,Jin X,Shen L,He Y,Wu X,Chen X,Sun C,Zheng M,Zhang R,Ye H,An H,Wong A

    更新日期:2020-02-28 00:00:00

  • Endosymbionts Alter Larva-to-Nymph Transstadial Transmission of Babesia microti in Rhipicephalus haemaphysaloides Ticks.

    abstract::Maternally inherited endosymbionts inhabit a variety of arthropods. Some of them can protect the arthropod host against a wide range of pathogens. However, very little is known about the association between endosymbionts and pathogen susceptibility in ticks. The present study investigated the effect of endosymbionts o...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2018.01415

    authors: Li LH,Zhang Y,Zhu D,Zhou XN

    更新日期:2018-06-27 00:00:00

  • Variant Signal Peptides of Vaccine Antigen, FHbp, Impair Processing Affecting Surface Localization and Antibody-Mediated Killing in Most Meningococcal Isolates.

    abstract::Meningococcal lipoprotein, Factor H binding protein (FHbp), is the sole antigen of the Trumenba vaccine (Pfizer) and one of four antigens of the Bexsero vaccine (GSK) targeting Neisseria meningitidis serogroup B isolates. Lipidation of FHbp is assumed to occur for all isolates. We show in the majority of a collection ...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2019.02847

    authors: da Silva RAG,Karlyshev AV,Oldfield NJ,Wooldridge KG,Bayliss CD,Ryan A,Griffin R

    更新日期:2019-12-19 00:00:00

  • Probiotic Lactobacillus rhamnosus GG Promotes Mouse Gut Microbiota Diversity and T Cell Differentiation.

    abstract::Lactic acid bacteria (LAB) are the primary genera of the intestinal flora and have many probiotic functions. In the present study, Lactobacillus rhamnosus GG (LGG) ATCC 53103 was used to treat BALB/c mice. After LGG intervention, both low and high LGG doses were shown to improve the observed OTU, Chao1, ACE, and Shann...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2020.607735

    authors: Shi CW,Cheng MY,Yang X,Lu YY,Yin HD,Zeng Y,Wang RY,Jiang YL,Yang WT,Wang JZ,Zhao DD,Huang HB,Ye LP,Cao X,Yang GL,Wang CF

    更新日期:2020-12-17 00:00:00

  • Characterization of the Culturable Subpopulations of Lactobacillus in the Chicken Intestinal Tract as a Resource for Probiotic Development.

    abstract::To gain better understanding of the distributions of the culturable Lactobacillus species in the chicken intestinal tract, we collected ceca, and distal ileum from 10 3-weeks-old broiler chickens. Lactobacillus strains from cecal lumen contents (M-CL), and those associated with mucosa of ceca (M-CM) and ileum (M-IM) w...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2017.01389

    authors: Adhikari B,Kwon YM

    更新日期:2017-07-26 00:00:00

  • Genomic analysis of six new Geobacillus strains reveals highly conserved carbohydrate degradation architectures and strategies.

    abstract::In this work we report the whole genome sequences of six new Geobacillus xylanolytic strains along with the genomic analysis of their capability to degrade carbohydrates. The six sequenced Geobacillus strains described here have a range of GC contents from 43.9% to 52.5% and clade with named Geobacillus species throug...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2015.00430

    authors: Brumm PJ,De Maayer P,Mead DA,Cowan DA

    更新日期:2015-05-12 00:00:00

  • Beyond 16S rRNA Community Profiling: Intra-Species Diversity in the Gut Microbiota.

    abstract::Interactions with microbes affect many aspects of animal biology, including immune system development, nutrition and health. In vertebrates, the gut microbiota is dominated by a small subset of phyla, but the species composition within these phyla is typically not conserved. Moreover, several recent studies have shown...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章,评审

    doi:10.3389/fmicb.2016.01475

    authors: Ellegaard KM,Engel P

    更新日期:2016-09-21 00:00:00

  • A New Pathway for Mannitol Metabolism in Yeasts Suggests a Link to the Evolution of Alcoholic Fermentation.

    abstract::The yeasts belonging to the Wickerhamiella and Starmerella genera (W/S clade) share a distinctive evolutionary history marked by loss and subsequent reinstatement of alcoholic fermentation mediated by horizontal gene transfer events. Species in this clade also share unusual features of metabolism, namely the preferenc...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2019.02510

    authors: Gonçalves C,Ferreira C,Gonçalves LG,Turner DL,Leandro MJ,Salema-Oom M,Santos H,Gonçalves P

    更新日期:2019-11-01 00:00:00

  • One System for All: Is Mass Spectrometry a Future Alternative for Conventional Antibiotic Susceptibility Testing?

    abstract::The two main pillars of clinical microbiological diagnostics are the identification of potentially pathogenic microorganisms from patient samples and the testing for antibiotic susceptibility (AST) to allow efficient treatment with active antimicrobial agents. While routine microbial species identification is increasi...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章,评审

    doi:10.3389/fmicb.2019.02711

    authors: Welker M,van Belkum A

    更新日期:2019-11-26 00:00:00

  • Corrigendum: Cross-Talk between Staphylococcus aureus and Other Staphylococcal Species via the agr Quorum Sensing System.

    abstract::[This corrects the article on p. 1733 in vol. 7, PMID: 27877157.]. ...

    journal_title:Frontiers in microbiology

    pub_type: 已发布勘误

    doi:10.3389/fmicb.2017.01075

    authors: Canovas J,Baldry M,Bojer MS,Andersen PS,Grzeskowiak PK,Stegger M,Damborg P,Olsen CA,Ingmer H

    更新日期:2017-06-08 00:00:00

  • PP2A-Like Protein Phosphatase (Sit4) Regulatory Subunits, Sap155 and Sap190, Regulate Candida albicans' Cell Growth, Morphogenesis, and Virulence.

    abstract::PP2A-like phosphatases share high homology with PP2A enzymes and are composed of a catalytic subunit and a regulatory subunit. In Candida albicans, the PP2A-like catalytic subunit SIT4 regulates cell growth, morphogenesis, and virulence. However, the functions of its regulatory subunits remain unclear. Here, by homolo...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2019.02943

    authors: Han Q,Pan C,Wang Y,Zhao L,Wang Y,Sang J

    更新日期:2019-12-20 00:00:00

  • Comparative Outer Membrane Protein Analysis of High and Low-Invasive Strains of Cronobacter malonaticus.

    abstract::Cronobacter are an important group of foodborne pathogens that has been linked to life-threatening infections in both infants and adults. The major infections associated with Cronobacter species are neonatal meningitis, necrotizing enterocolitis, and septicaemia. There are seven species in the Cronobacter genus, of wh...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2017.02268

    authors: Aldubyan MA,Almami IS,Benslimane FM,Alsonosi AM,Forsythe SJ

    更新日期:2017-11-17 00:00:00

  • Early Trypanosoma cruzi Infection Triggers mTORC1-Mediated Respiration Increase and Mitochondrial Biogenesis in Human Primary Cardiomyocytes.

    abstract::Chagasic chronic cardiomyopathy is one of the most frequent and severe manifestations of Chagas disease, caused by the parasite Trypanosoma cruzi. The pathogenic and biochemical mechanisms responsible for cardiac lesions remain not completely understood, although it is clear that hypertrophy and subsequent heart dilat...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2018.01889

    authors: Libisch MG,Faral-Tello P,Garg NJ,Radi R,Piacenza L,Robello C

    更新日期:2018-08-16 00:00:00

  • PVP-SVM: Sequence-Based Prediction of Phage Virion Proteins Using a Support Vector Machine.

    abstract::Accurately identifying bacteriophage virion proteins from uncharacterized sequences is important to understand interactions between the phage and its host bacteria in order to develop new antibacterial drugs. However, identification of such proteins using experimental techniques is expensive and often time consuming; ...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2018.00476

    authors: Manavalan B,Shin TH,Lee G

    更新日期:2018-03-16 00:00:00

  • Production of Ramoplanin and Ramoplanin Analogs by Actinomycetes.

    abstract::Ramoplanin is a glycolipodepsipeptide antibiotic obtained from fermentation of Actinoplanes sp. ATCC 33076 that exhibits activity against clinically important multi-drug-resistant, Gram-positive pathogens including vancomycin-resistant Enterococcus (VRE), methicillin-resistant Staphylococcus aureus (MRSA), and vancomy...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2017.00343

    authors: de la Cruz M,González I,Parish CA,Onishi R,Tormo JR,Martín J,Peláez F,Zink D,El Aouad N,Reyes F,Genilloud O,Vicente F

    更新日期:2017-03-06 00:00:00

  • Characterization of genetic structures of the QepA3 gene in clinical isolates of Enterobacteriaceae.

    abstract::QepA is one of the genes that confer quinolone resistance in bacteria. The aim of this study was to analyze the genetic structures of plasmids that carry a qepA3, a recently discovered allele of qepA in Enterobacteriaceae clinical isolates. 656 non-redundant Enterobacteriaceae clinical isolates were screened for the q...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2015.01147

    authors: Wang D,Huang X,Chen J,Mou Y,Li H,Yang L

    更新日期:2015-10-15 00:00:00

  • Anti-outer Membrane Vesicle Antibodies Increase Antibiotic Sensitivity of Pan-Drug-Resistant Acinetobacter baumannii.

    abstract::Acinetobacter baumannii often causes serious nosocomial infections. Because of its serious drug resistance problems, complex drug resistance mechanism, and rapid adaptation to antibiotics, it often shows pan-drug resistance and high fatality rates, which represent great challenges for clinical treatment. Therefore, id...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2019.01379

    authors: Huang W,Zhang Q,Li W,Chen Y,Shu C,Li Q,Zhou J,Ye C,Bai H,Sun W,Yang X,Ma Y

    更新日期:2019-06-18 00:00:00

  • The Impact of ackA, pta, and ackA-pta Mutations on Growth, Gene Expression and Protein Acetylation in Escherichia coli K-12.

    abstract::Acetate is a characteristic by-product of Escherichia coli K-12 growing in batch cultures with glucose, both under aerobic as well as anaerobic conditions. While the reason underlying aerobic acetate production is still under discussion, during anaerobic growth acetate production is important for ATP generation by sub...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2020.00233

    authors: Schütze A,Benndorf D,Püttker S,Kohrs F,Bettenbrock K

    更新日期:2020-02-21 00:00:00

  • Different metabolic features of Bacteroides fragilis growing in the presence of glucose and exopolysaccharides of bifidobacteria.

    abstract::Bacteroides is among the most abundant microorganism inhabiting the human intestine. They are saccharolytic bacteria able to use dietary or host-derived glycans as energy sources. Some Bacteroides fragilis strains contribute to the maturation of the immune system but it is also an opportunistic pathogen. The intestine...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2015.00825

    authors: Rios-Covian D,Sánchez B,Salazar N,Martínez N,Redruello B,Gueimonde M,de Los Reyes-Gavilán CG

    更新日期:2015-08-18 00:00:00

  • Differential Regulation of Myeloid-Derived Suppressor Cells by Candida Species.

    abstract::Myeloid-derived suppressor cells (MDSCs) are innate immune cells characterized by their ability to suppress T-cell responses. Recently, we demonstrated that the human-pathogenic fungi Candida albicans and Aspergillus fumigatus induced a distinct subset of neutrophilic MDSCs. To dissect Candida-mediated MDSC induction ...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2016.01624

    authors: Singh A,Lelis F,Braig S,Schäfer I,Hartl D,Rieber N

    更新日期:2016-10-13 00:00:00

  • Detection of Colistin Resistance in Salmonella enterica Using MALDIxin Test on the Routine MALDI Biotyper Sirius Mass Spectrometer.

    abstract::Resistance to polymyxins in most Gram-negative bacteria arises from chemical modifications to the lipid A portion of their lipopolysaccharide (LPS) mediated by chromosomally encoded mutations or the recently discovered plasmid-encoded mcr genes that have further complicated the landscape of colistin resistance. Curren...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2020.01141

    authors: Dortet L,Bonnin RA,Le Hello S,Fabre L,Bonnet R,Kostrzewa M,Filloux A,Larrouy-Maumus G

    更新日期:2020-06-03 00:00:00

  • The Role of Volatile Organic Compounds and Rhizosphere Competence in Mode of Action of the Non-pathogenic Fusarium oxysporum FO12 Toward Verticillium Wilt.

    abstract::Verticillium wilts caused by Verticillium spp. are among the most challenging plant diseases to control and affect numerous hosts worldwide. Due to the lack of effective, conventional control methods, integrated control strategies provide a promising approach to manage these diseases. The non-pathogenic Fusarium oxysp...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2019.01808

    authors: Mulero-Aparicio A,Cernava T,Turrà D,Schaefer A,Di Pietro A,López-Escudero FJ,Trapero A,Berg G

    更新日期:2019-08-02 00:00:00

  • Reconstruction of the Regulatory Network for Bacillus subtilis and Reconciliation with Gene Expression Data.

    abstract::We introduce a manually constructed and curated regulatory network model that describes the current state of knowledge of transcriptional regulation of Bacillus subtilis. The model corresponds to an updated and enlarged version of the regulatory model of central metabolism originally proposed in 2008. We extended the ...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2016.00275

    authors: Faria JP,Overbeek R,Taylor RC,Conrad N,Vonstein V,Goelzer A,Fromion V,Rocha M,Rocha I,Henry CS

    更新日期:2016-03-18 00:00:00

  • Bacterioplankton Dynamics within a Large Anthropogenically Impacted Urban Estuary.

    abstract::The abundant and diverse microorganisms that inhabit aquatic systems are both determinants and indicators of aquatic health, providing essential ecosystem services such as nutrient cycling but also causing harmful blooms and disease in impacted habitats. Estuaries are among the most urbanized coastal ecosystems and as...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2015.01438

    authors: Jeffries TC,Schmitz Fontes ML,Harrison DP,Van-Dongen-Vogels V,Eyre BD,Ralph PJ,Seymour JR

    更新日期:2016-01-26 00:00:00

  • Identification of New Degrons in Streptococcus mutans Reveals a Novel Strategy for Engineering Targeted, Controllable Proteolysis.

    abstract::Recently, controllable, targeted proteolysis has emerged as one of the most promising new strategies to study essential genes and otherwise toxic mutations. One of the principal limitations preventing the wider adoption of this approach is due to the lack of easily identifiable species-specific degrons that can be use...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2017.02572

    authors: Liu N,Chaudhry MT,Xie Z,Kreth J,Merritt J

    更新日期:2017-12-19 00:00:00

  • In vitro Evaluation of Photodynamic Effects Against Biofilms of Dermatophytes Involved in Onychomycosis.

    abstract::Dermatophytes are the most common cause of onychomycosis, counting for 90% fungal nail infection. Although dermatophyte pathogens are normally susceptible to antifungal agents, onychomycosis often results in refractory chronic disease, and the formation of biofilms frequently underlines the inadequate responses and re...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2019.01228

    authors: Chen B,Sun Y,Zhang J,Chen R,Zhong X,Wu X,Zheng L,Zhao J

    更新日期:2019-06-07 00:00:00

  • Swine Manure Composting With Compound Microbial Inoculants: Removal of Antibiotic Resistance Genes and Their Associations With Microbial Community.

    abstract::In this study, compound microbial inoculants, including three Bacillus strains and one Yeast strain, were inoculated into swine manure composting to explore the effects on antibiotic resistance genes (ARGs) and mobile genetic elements (MGEs), microbial community structure, and pathogenic bacteria. The results indicate...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2020.592592

    authors: Li K,Cao R,Mo S,Yao R,Ren Z,Wu J

    更新日期:2020-11-03 00:00:00