Vibrio cholerae at the Intersection of Immunity and the Microbiome.

abstract：:Globally, more antimicrobials are used in food-producing animals than in humans, and the extensive use of medically important human antimicrobials poses a significant public health threat in the face of rising antimicrobial resistance (AMR). The development of novel ionophores, a class of antimicrobials used exclusive...

journal_title：mSphere

authors： Harbison-Price N,Ferguson SA,Heikal A,Taiaroa G,Hards K,Nakatani Y,Rennison D,Brimble MA,El-Deeb IM,Bohlmann L,McDevitt CA,von Itzstein M,Walker MJ,Cook GM

更新日期：2020-03-18 00:00:00

abstract：:We are exposed daily to many glycans from bacteria and food plants. Bacterial glycans are generally antigenic and elicit antibody responses. It is unclear if food glycans' sharing of antigens with bacterial glycans influences our immune responses to bacteria. We studied 14 different plant foods for cross-reactivity wi...

journal_title：mSphere

authors： Nahm MH,Yu J,Vlach J,Bar-Peled M

更新日期：2020-04-08 00:00:00

abstract：:Enveloped viruses require viral fusion proteins to promote fusion of the viral envelope with a target cell membrane. To drive fusion, these proteins undergo large conformational changes that must occur at the right place and at the right time. Understanding the elements which control the stability of the prefusion sta...

journal_title：mSphere

authors： Webb SR,Smith SE,Fried MG,Dutch RE

更新日期：2018-04-18 00:00:00

abstract：:Many coronavirus disease 2019 (COVID-19) patients demonstrate lethal respiratory complications caused by cytokine release syndrome (CRS). Multiple cytokines have been implicated in CRS, but levels of tumor necrosis factor superfamily 14 (TNFSF14) (LIGHT) have not been previously measured in this setting. In this study...

journal_title：mSphere

authors： Perlin DS,Zafir-Lavie I,Roadcap L,Raines S,Ware CF,Neil GA

更新日期：2020-08-12 00:00:00

abstract：:Chikungunya virus (CHIKV) and Zika virus (ZIKV) emerged in the Americas in 2013. Limited antigenic variability of CHIKV and ZIKV may restrict urban transmission cycles due to population protective immunity. In Africa, sylvatic transmission cycles involving nonhuman primates (NHP) are known for CHIKV and ZIKV, causing ...

journal_title：mSphere

authors： Moreira-Soto A,Carneiro IO,Fischer C,Feldmann M,Kümmerer BM,Silva NS,Santos UG,Souza BFCD,Liborio FA,Valença-Montenegro MM,Laroque PO,da Fontoura FR,Oliveira AVD,Drosten C,de Lamballerie X,Franke CR,Drexler JF

更新日期：2018-01-31 00:00:00

abstract：:Candida albicans, a pervasive opportunistic pathogen, undergoes a unique phenotypic transition from a "white" phenotype to an "opaque" phenotype. The switch to opaque impacts gene expression, cell morphology, wall structure, metabolism, biofilm formation, mating, virulence, and colonization of the skin and gastrointes...

journal_title：mSphere

authors： Park YN,Pujol C,Wessels DJ,Soll DR

更新日期：2020-09-23 00:00:00

abstract：:Michael D. L. Johnson is a molecular microbiologist observing the role of metals in bacterial biology. In this mSphere of Influence article, he discusses the impact that the paper "Decoupling of the minority PhD talent pool and assistant professor hiring in medical school basic science departments in the US" by Kennet...

journal_title：mSphere

authors： Johnson MDL

更新日期：2019-09-18 00:00:00

abstract：:Staphylococcus aureus is a facultative intracellular pathogen that invades and replicates within many types of phagocytic and nonphagocytic cells. During intracellular infection, S. aureus is capable of subverting xenophagy and escaping to the cytosol of the host cell. Furthermore, drug-induced autophagy facilitates t...

journal_title：mSphere

authors： Bravo-Santano N,Ellis JK,Mateos LM,Calle Y,Keun HC,Behrends V,Letek M

更新日期：2018-08-08 00:00:00

abstract：:Cryptococcus neoformans and related species are encapsulated basidiomycetous fungi that cause meningoencephalitis in individuals with immune deficiency. This pathogen has a tractable genetic system; however, gene disruption via electroporation remains difficult, while biolistic transformation is often limited by lack ...

journal_title：mSphere

authors： Wang P

更新日期：2018-06-13 00:00:00

abstract：:Female Hawaiian bobtail squid, Euprymna scolopes, harbor a symbiotic bacterial community in a reproductive organ, the accessory nidamental gland (ANG). This community is known to be stable over several generations of wild-caught bobtail squid but has, to date, been examined for only one population in Maunalua Bay, Oah...

journal_title：mSphere

authors： Kerwin AH,Nyholm SV

更新日期：2018-03-28 00:00:00

abstract：:Spores, the infectious agents of many Firmicutes, are remarkably resilient cell forms. Even distant relatives can have similar spore architectures although some display unique features; they all incorporate protective proteinaceous envelopes. We previously found that Bacillus spores can achieve these protective proper...

journal_title：mSphere

authors： Janganan TK,Mullin N,Dafis-Sagarmendi A,Brunt J,Tzokov SB,Stringer S,Moir A,Chaudhuri RR,Fagan RP,Hobbs JK,Bullough PA

更新日期：2020-07-01 00:00:00

abstract：:Toxoplasma gondii can infect and replicate in vascular endothelial cells prior to entering host tissues. However, little is known about the molecular interactions at the parasite-endothelial cell interface. We demonstrate that T. gondii infection of primary human umbilical vein endothelial cells (HUVEC) altered cell m...

journal_title：mSphere

authors： Franklin-Murray AL,Mallya S,Jankeel A,Sureshchandra S,Messaoudi I,Lodoen MB

更新日期：2020-01-29 00:00:00

abstract：:Polymyxin B and colistin are exclusively active against Gram-negative pathogens and have been used in the clinic as a last-line therapy. In this study, we investigated the antimicrobial activity of a novel polymyxin, FADDI-019, against Staphylococcus aureus. MIC and time-kill assays were employed to measure the activi...

journal_title：mSphere

authors： Zhao J,Cheah SE,Roberts KD,Nation RL,Thompson PE,Velkov T,Du Z,Johnson MD,Li J

更新日期：2016-07-27 00:00:00

abstract：:Buruli ulcer is a neglected tropical disease of skin and subcutaneous tissue caused by infection with the pathogen Mycobacterium ulcerans Many critical issues for disease control, such as understanding the mode of transmission and identifying source reservoirs of M. ulcerans, are still largely unknown. Here, we used g...

journal_title：mSphere

authors： Vandelannoote K,Phanzu DM,Kibadi K,Eddyani M,Meehan CJ,Jordaens K,Leirs H,Portaels F,Stinear TP,Harris SR,de Jong BC

更新日期：2019-02-06 00:00:00

abstract：:Cryptococcus neoformans is a pathogenic yeast that causes lethal cryptococcal meningitis in immunocompromised patients. One of the challenges in treating cryptococcosis is the development of resistance to azole antifungals. Previous studies linked azole resistance to elevated numbers of copies of critical resistance g...

journal_title：mSphere

authors： Altamirano S,Fang D,Simmons C,Sridhar S,Wu P,Sanyal K,Kozubowski L

更新日期：2017-06-14 00:00:00

abstract：:Molecular detection of biological agents in the field has traditionally relied on the use of quantitative real-time PCR (qPCR), which now includes commercially available instruments that can be used in the laboratory or field. Adapting this technology for field-forward applications necessitated innovation to minimize ...

journal_title：mSphere

authors： Yeh KB,Wood H,Scullion M,Russell JA,Parker K,Gnade BT,Jones AR,Whittier C,Mereish K

更新日期：2019-12-11 00:00:00

abstract：:Coxsackievirus typically infects humans via the gastrointestinal tract, which has a large number of microorganisms collectively referred to as the microbiota. To study how the intestinal microbiota influences enteric virus infection, several groups have used an antibiotic regimen in mice to deplete bacteria. These stu...

journal_title：mSphere

authors： Woods Acevedo MA,Erickson AK,Pfeiffer JK

更新日期：2019-02-20 00:00:00

abstract：:Manipulation of the host cell is a crucial part of life for many intracellular organisms. We have recently come to appreciate the extent to which the intracellular pathogen Toxoplasma gondii reprograms its host cell, and this is illustrated by the marked upregulation of the central regulator c-Myc, an oncogene that co...

journal_title：mSphere

authors： Panas MW,Boothroyd JC

更新日期：2020-06-24 00:00:00

abstract：:Mary Lopez-Perez works on immunology and pathogenesis of malaria. In this mSphere of Influence article, she reflects on how the paper "Functional antibodies against VAR2CSA in nonpregnant populations from Colombia exposed to Plasmodium falciparum and Plasmodium vivax" by S. Gnidehou, J. Doritchamou, E. M. Arango, A. C...

journal_title：mSphere

authors： Lopez-Perez M

更新日期：2020-04-08 00:00:00

abstract：:Continued influx of metagenome-derived proteins with misannotated taxonomy into conventional databases, including RefSeq, threatens to eliminate the value of taxonomy identifiers. To prevent this, urgent efforts should be undertaken by submitters of metagenomic data sets as well as by database managers. ...

journal_title：mSphere

authors： Arkhipova IR

更新日期：2020-11-04 00:00:00

abstract：:Bordetella pertussis, B. parapertussis, and B. bronchiseptica cause respiratory infections, many of which are characterized by coughing of the infected hosts. The pathogenesis of the coughing remains to be analyzed, mainly because there were no convenient infection models of small animals that replicate coughing after...

journal_title：mSphere

authors： Nakamura K,Shinoda N,Hiramatsu Y,Ohnishi S,Kamitani S,Ogura Y,Hayashi T,Horiguchi Y

更新日期：2019-04-24 00:00:00

abstract：:Chlamydia trachomatis delivers multiple type 3 secreted effector proteins to host epithelial cells to manipulate cytoskeletal functions, membrane dynamics, and signaling pathways. TepP is the most abundant effector protein secreted early in infection, but its molecular function is poorly understood. In this report, we...

journal_title：mSphere

authors： Carpenter V,Chen YS,Dolat L,Valdivia RH

更新日期：2017-07-19 00:00:00

abstract：:The wide distribution of colistin-resistant bacteria in developing countries has become a common phenomenon. To understand the mechanisms underlying their distribution, we studied the mcr genetic background of colistin-resistant Escherichia coli isolates from the fecal microbiota of healthy human residents from a comm...

journal_title：mSphere

authors： Yamaguchi T,Kawahara R,Hamamoto K,Hirai I,Khong DT,Nguyen TN,Tran HT,Motooka D,Nakamura S,Yamamoto Y

更新日期：2020-03-04 00:00:00

abstract：:Streptococcus pneumoniae (the pneumococcus) carriage is commonly used to measure effects of pneumococcal vaccines. Based on findings from culture-based studies, the World Health Organization recommends both nasopharyngeal (NP) and oropharyngeal (OP) sampling for detecting adult carriage. Given evidence of potential co...

journal_title：mSphere

authors： Boelsen LK,Dunne EM,Gould KA,Ratu FT,Vidal JE,Russell FM,Mulholland EK,Hinds J,Satzke C

更新日期：2020-07-29 00:00:00

abstract：:Clinical isolates of Pseudomonas aeruginosa from patients with cystic fibrosis (CF) are known to differ from those associated with non-CF hosts by colony morphology, drug susceptibility patterns, and genomic hypermutability. Pseudomonas aeruginosa isolates from CF patients have long been recognized for their overall r...

journal_title：mSphere

authors： Qin X,Zhou C,Zerr DM,Adler A,Addetia A,Yuan S,Greninger AL

更新日期：2018-03-14 00:00:00

abstract：:Père David's deer (Elaphurus davidianus or milu) is an endangered species, and the prevalence of antimicrobial resistance (AMR) such as mcr-1-positive strains among them has been unknown. In this study, we aimed to investigate the genomic characterizations of mcr-1-positive strains and provide insight into the dissemi...

journal_title：mSphere

authors： Lu X,Xiao X,Liu Y,Huang S,Li R,Wang Z

更新日期：2020-12-23 00:00:00

abstract：:Regular protocols for the isolation of fungal extracellular vesicles (EVs) are time-consuming, hard to reproduce, and produce low yields. In an attempt to improve the protocols used for EV isolation, we explored a model of vesicle production after growth of Cryptococcus gattii and Cryptococcus neoformans on solid medi...

journal_title：mSphere

authors： Reis FCG,Borges BS,Jozefowicz LJ,Sena BAG,Garcia AWA,Medeiros LC,Martins ST,Honorato L,Schrank A,Vainstein MH,Kmetzsch L,Nimrichter L,Alves LR,Staats CC,Rodrigues ML

更新日期：2019-03-20 00:00:00

abstract：:Salmonella genomic island 1 (SGI1) is an integrative genetic island first described in Salmonella enterica serovars Typhimurium DT104 and Agona in 2000. Variants of it have since been described in multiple serovars of S. enterica, as well as in Proteus mirabilis, Acinetobacter baumannii, Morganella morganii, and sever...

journal_title：mSphere

authors： Cummins ML,Roy Chowdhury P,Marenda MS,Browning GF,Djordjevic SP

更新日期：2019-05-22 00:00:00

abstract：:Increased knowledge about the role of horizontal gene transfer is key to improve our understanding of the spread of antimicrobial resistance (AMR) in human populations. We therefore studied the dissemination of the blaCTX-M-15 extended-spectrum-β-lactamase (ESBL) gene in Klebsiella pneumoniae isolates obtained from st...

journal_title：mSphere

authors： Pedersen T,Tellevik MG,Kommedal Ø,Lindemann PC,Moyo SJ,Janice J,Blomberg B,Samuelsen Ø,Langeland N

更新日期：2020-07-15 00:00:00

abstract：:Cryptococcus gattii R265 is a hypervirulent fungal strain responsible for the recent outbreak of cryptococcosis in Vancouver Island of British Columbia in Canada. It differs significantly from Cryptococcus neoformans in its natural environment, its preferred site in the mammalian host, and its pathogenesis. Our previo...

journal_title：mSphere

authors： Lam WC,Upadhya R,Specht CA,Ragsdale AE,Hole CR,Levitz SM,Lodge JK

更新日期：2019-10-09 00:00:00