Abstract：
Objective:To evaluate the association of miR-196a rs11614913 C/T genetic variation and its target gene annexin A1 mRNA expression with breast cancer risk in Pakistani female ethnicities. Methods:This case control study, conducted from March 2017 to November 2018 included 295 breast cancer patients, 295 controls of three Pakistani ethnicities and archived 100 samples of cohort group for genotyping and expression profiling. Genotyping of miR-196a (rs11614913 C/T) was done by ARMS PCR technique. Annexin-A1 (ANXA1) mRNA expression was measured with qRT-PCR and detection of protein expression of ANXA1 was done by immunohistochemistry. Results:CC homozygous genotype of miR-196a rs11614913 was present in 81.4% of cases and 73.9% controls. C/T polymorphism was found to be significantly associated with decrease risk of breast cancer (OR=0.25 (0.11- 0.58, p <0.05). Similar trend was seen with the minor T allele (OR=0.55 (0.39-0.77, p <0.05, and both dominant and recessive models (OR=0.64; p=0.02 and OR=0.26, p=0.00). In the KPK ethnic group significant decrease association with breast cancer risk was observed (OR= 0.22 (0.09-0.53, p < 0.05). Immunohistochemical staining showed loss of ANXA1 protein expression in 72 samples, and significant association was observed with pathological type p=0. 00 and triple negative receptor status p=0.03 and with genotypes of miR-196ap=0.00. Increase relative expression of 2.81± .88 by qPCR analysis of ANXA1 mRNA was noted with TT genotype. Conclusions:Our results demonstrate that miR-196a rs11614913 C/T polymorphism is associated with a decreased risk and loss of protein expression in breast cancer in the Pakistani population.
journal_name
Pak J Med Scijournal_title
Pakistan journal of medical sciencesauthors
Rahim A,Afzal M,Naveed AKdoi
10.12669/pjms.35.6.1322subject
Has Abstractpub_date
2019-11-01 00:00:00pages
1598-1604issue
6eissn
1682-024Xissn
1681-715Xpii
PJMS-35-1598journal_volume
35pub_type
杂志文章abstract：OBJECTIVE:The objective of the current study was to establish the reference ranges of haematological indices amongst five healthy infantile (<1 year) age groups. METHODOLOGY:It was a descriptive cross sectional study carried out at the Department of Haematology, Armed Forces Institute of Pathology (AFIP), Rawalpindi. ...
journal_title：Pakistan journal of medical sciences
pub_type： 杂志文章
doi：10.12669/pjms.292.3067
更新日期：2013-04-01 00:00:00
abstract：OBJECTIVE:This study investigated the relationship between cardiorespiratory fitness (CRF) and adiposity in young adults. METHODS:Data was collected from 133 students of a medical college of Pakistan. The study was conducted on young adults, aged 17-24 years, recruited from Jinnah Medical & Dental College, Karachi, be...
journal_title：Pakistan journal of medical sciences
pub_type： 杂志文章
doi：10.12669/pjms.333.12294
更新日期：2017-05-01 00:00:00
abstract：OBJECTIVE:To evaluate the frequency of fetomaternal outcome of pregnancy with Mitral stenosis admitted in Civil Hospital Karachi. METHODS:It was a two years descriptive study done in the Department of Obstetrics and Gynaecology Civil Hospital Karachi. All pregnant women with a known or newly diagnosed Mitral stenosis ...
journal_title：Pakistan journal of medical sciences
pub_type： 杂志文章
doi：10.12669/pjms.313.7020
更新日期：2015-01-01 00:00:00
abstract：Objectives:To evaluate the relationship of D-dimer, CD147 and miR-203, and detect the influence of these biomarkers on the pathological characteristics in patients with gastric cancer. Methods:The patients with gastric cancer treated using radical gastrectomy between May 2013 and October 2017 were reviewed retrospecti...
journal_title：Pakistan journal of medical sciences
pub_type： 杂志文章
doi：10.12669/pjms.35.2.718
更新日期：2019-03-01 00:00:00
abstract：Objectives:To understand and catalogue the specific determinants of this alarming rate of malnutrition among children of Tharparkar district, Sindh Pakistan. Methods:This was a hospital based analytical survey. Data was collected through a semi-structured questionnaire by interviewing mothers of the children (age 6-59...
journal_title：Pakistan journal of medical sciences
pub_type： 杂志文章
doi：10.12669/pjms.342.14977
更新日期：2018-03-01 00:00:00
abstract：OBJECTIVE:This is aimed at investigating some coagulation and haematologic profile of HIV positive patients on highly active antiretroviral therapy in patients attending clinic at the University of Benin Teaching Hospital. METHODS:This is a correlation study comprising fifty (50) HIV positive patients on HAART between...
journal_title：Pakistan journal of medical sciences
pub_type： 杂志文章
doi：10.12669/pjms.316.7943
更新日期：2015-11-01 00:00:00
abstract：OBJECTIVE:To explore the correlation between magnetic resonance imaging (MRI) features of uterine fibroids (UFs) and therapeutic effects of high-intensity focused ultrasound ablation (HIFUA), and to provide evidence for UFs diagnosis with MRI in clinical practice. METHODS:Forty-three UFs patients who were treated in o...
journal_title：Pakistan journal of medical sciences
pub_type： 杂志文章
doi：10.12669/pjms.314.7294
更新日期：2015-07-01 00:00:00
abstract：Objective:To investigate the relationship between myocardial enzymes, liver function and metabolic acidosis in children with rotavirus infection diarrhea. Methods:The data of 70 children with infectious diarrhea treated in Baoding Children's Hospital, China, from October 2017 to April 2018 were retrospectively studied...
journal_title：Pakistan journal of medical sciences
pub_type： 杂志文章
doi：10.12669/pjms.36.6.2325
更新日期：2020-09-01 00:00:00
abstract：OBJECTIVE:To determine the various spectrum of pancytopenia with its frequency on the basis of bone marrow examination in children from 6 months to 14 years. METHODS:A retrospective descriptive study was carried out at Department of Pediatric Rehman Medical Institute Peshawar from January 2006 to December 2012. A tota...
journal_title：Pakistan journal of medical sciences
pub_type： 杂志文章
doi：10.12669/pjms.295.3865
更新日期：2013-09-01 00:00:00
abstract：OBJECTIVE:To explore the factors associated with preoperative epileptic seizure and surgical outcome in patients with cerebral gangliolioma (GG). METHODS:A total of 31 consecutive patients with pathologically confirmed ganglioglioma and surgically treated from January 2003 to June 2011 in West China Hospital of Sichua...
journal_title：Pakistan journal of medical sciences
pub_type： 杂志文章
doi：10.12669/pjms.302.4362
更新日期：2014-03-01 00:00:00
abstract：OBJECTIVE:To evaluate the prevalence and the individual components of metabolic syndrome (MetS) in Jordanian adults. METHODS:In this cross sectional study, 630 adult subjects (308 men and 322 women) aged between 20-70 years were recruited from the clinics at the King Hussein Medical Center. The diagnosis of MetS was m...
journal_title：Pakistan journal of medical sciences
pub_type： 杂志文章
doi：10.12669/pjms.316.7714
更新日期：2015-11-01 00:00:00
abstract：OBJECTIVE:To isolate Helicobacter pylori (HP) from the dental plaque of a selected group of children and to compare the oral and salivary findings of patients with those of a healthy control group. METHODS:A total of 70 children aged 5-15 years were included in this study. An intraoral examination was performed for ea...
journal_title：Pakistan journal of medical sciences
pub_type： 杂志文章
doi：10.12669/pjms.324.10034
更新日期：2016-07-01 00:00:00
abstract：Objectives:Although Spinal anesthesia is the most common and safe anesthetic method for patients undergoing cesarean section, difficult access to it is a frequent problem in operating theaters. The predictive factors for the difficulty of spinal anesthesia in patients undergoing cesarean section were investigated. Met...
journal_title：Pakistan journal of medical sciences
pub_type： 杂志文章
doi：10.12669/pjms.35.6.1276
更新日期：2019-11-01 00:00:00
abstract：Background and Objective:Increased neutrophil lymphocyte ratio (NLR) is a marker as well as predictor of various cardiac and non cardiac disorders. Our aim was to assess the relationship between NLR and different level of glycemic control in type 2 diabetic patients. Methods:An observational study was conducted at dia...
journal_title：Pakistan journal of medical sciences
pub_type： 杂志文章
doi：10.12669/pjms.336.12900
更新日期：2017-11-01 00:00:00
abstract：Objective:To explore the value of gastrointestinal endoscopy in the early diagnosis and treatment of gastrointestinal tumors and lay a foundation for the diagnosis and treatment of gastrointestinal tumors. Methods:One hundred and eight patients with gastrointestinal tumors who were admitted to our hospital from August...
journal_title：Pakistan journal of medical sciences
pub_type： 杂志文章
doi：10.12669/pjms.36.2.707
更新日期：2020-01-01 00:00:00
abstract：Objective:Zinc is a vital trace element and its deficiency in cirrhosis might potentiate the development of hepatic encephalopathy. The objective of this study was to assess the zinc levels in serum of patients having viral cirrhosis and compare it with normal healthy controls. Methods:This study was conducted in Depa...
journal_title：Pakistan journal of medical sciences
pub_type： 杂志文章
doi：10.12669/pjms.342.14457
更新日期：2018-03-01 00:00:00
abstract：Objective:To investigate the prevalence of obesity and associated factors during childhood in Southeastern Turkey. Another objective was to determine the cut-off points of Waist to Height Ratio (WHtR) values for defining obesity/abdominal obesity. Methods:The community-based descriptive cross-sectional study was condu...
journal_title：Pakistan journal of medical sciences
pub_type： 杂志文章
doi：10.12669/pjms.35.6.748
更新日期：2019-11-01 00:00:00
abstract：Objective:To understand the most prominent factors contributing to job burnout in the nursing profession. Methods:Mixed method design was used in this study. In the qualitative part of the study, a focus group discussion approach was used to determine the major factors contributing in nurses' job burnout. The quantita...
journal_title：Pakistan journal of medical sciences
pub_type： 杂志文章
doi：10.12669/pjms.35.4.600
更新日期：2019-07-01 00:00:00
abstract：:Miller Fisher's syndrome (MFS) commonly presents in the fourth and fifth decades and are rare in people over 70 years. An 85-year-old female with no significant medical history presented with upper extremity anesthesia, ptosis, and unsteady gait. The patient had a history of hypertension and diabetes mellitus. Physica...
journal_title：Pakistan journal of medical sciences
pub_type：
doi：10.12669/pjms.295.3793
更新日期：2013-09-01 00:00:00
abstract：BACKGROUND AND OBJECTIVE:Hyperlipidemia and dyslipidemia are very common conditions among patients with Type-2 diabetes mellitus (T2DM) and associated with increased risk of coronary heart diseases. Physical activity and exercises along with medical management and dietary plan are common strategies to use for the manag...
journal_title：Pakistan journal of medical sciences
pub_type： 杂志文章
doi：10.12669/pjms.331.11758
更新日期：2017-01-01 00:00:00
abstract：Objective:To compare the effects of high-intensity laser therapy (HILT) and a combination of transcutaneous nerve stimulation (TENS) with ultrasound (US) therapy on pain and functionality in patients with chronic lumbar radiculopathy. Methods:This prospective randomized comparative study was conducted in Department of...
journal_title：Pakistan journal of medical sciences
pub_type： 杂志文章
doi：10.12669/pjms.343.14345
更新日期：2018-05-01 00:00:00
abstract：Objectives:To estimate the incidence of microvascular complications among subjects with type 2 diabetes at a tertiary care hospital. Methods:This retrospective longitudinal follow-up study assessed the data records of type 2 diabetic subjects who visited the outpatient department of Baqai Institute of Diabetology and ...
journal_title：Pakistan journal of medical sciences
pub_type： 杂志文章
doi：10.12669/pjms.345.15224
更新日期：2018-09-01 00:00:00
abstract：Objectives:Epilepsy is one of the most common chronic neurologic disorders in childhood and it affects 0.5-1% of children. The purpose of the study was to determine the prevalence and types of structural abnormalities in the epileptic children. Methods:The study was performed in Near East University and Dr. Suat Gunse...
journal_title：Pakistan journal of medical sciences
pub_type： 杂志文章
doi：10.12669/pjms.342.14807
更新日期：2018-03-01 00:00:00
abstract：Objectives:The value of gastric residual volume (GRV) monitoring in ventilator-associated pneumonia (VAP) has frequently been questioned in the past years. In this trial, the effect of GRV on the frequency of VAP was evaluated in critically ill patients under mechanical ventilation. Methods:This descriptive study was ...
journal_title：Pakistan journal of medical sciences
pub_type： 杂志文章
doi：10.12669/pjms.36.2.1321
更新日期：2020-01-01 00:00:00
abstract：OBJECTIVE:To determine site specific carotid intima-media thickness: common-carotid artery and carotid bifurcation in hypercholesterolemia patients as a marker for atherosclerosis. METHODS:Fifty patients with hypercholesterolemia and twenty controls were selected after getting informed consent regarding the investigat...
journal_title：Pakistan journal of medical sciences
pub_type： 杂志文章
doi：10.12669/pjms.295.3830
更新日期：2013-09-01 00:00:00
abstract：OBJECTIVE:Ectopic pregnancy in the interstitial part of the Fallopian tubes can be life-threatining considering the thin myometrial tissue surrounding the gestational sac and highly vascularization which may result in catastrophic haemorrhage when interstitium is ruptured. The diagnosis and management is challenging. C...
journal_title：Pakistan journal of medical sciences
pub_type： 杂志文章
doi：10.12669/pjms.332.12093
更新日期：2017-03-01 00:00:00
abstract：OBJECTIVE:To determine the knowledge, misconceptions and motivations towards blood donation among university students in KSA. METHODS:This cross sectional study was carried out at the King Abdulaziz University, Rabigh campus, Jeddah, KSA. A total of 326 adult males were interviewed and each individual completed a ques...
journal_title：Pakistan journal of medical sciences
pub_type： 杂志文章
doi：10.12669/pjms.296.4137
更新日期：2013-11-01 00:00:00
abstract：Objectives:To measure correlation and concordance between measured LDL cholesterol (mLDLc) and Friedewald's calculated LDL cholesterol (cLDLc). To compare the mLDLc and cLDLc values for various anthropometric measures and biochemical indices including insulin resistance, nephropathy, glycated hemoglobin and triglycerid...
journal_title：Pakistan journal of medical sciences
pub_type： 杂志文章
doi：10.12669/pjms.35.2.679
更新日期：2019-03-01 00:00:00
abstract：OBJECTIVE:To determine faculty perception on journal club (JC) presentation at Bahria University Medical and Dental College (BUMDC), Karachi. Pakistan. METHODS:It was a cross sectional study conducted from January 2009 to December 2012 to acquire faculty member's feedback on JC presentations in structured meeting at B...
journal_title：Pakistan journal of medical sciences
pub_type： 杂志文章
doi：10.12669/pjms.311.6314
更新日期：2015-01-01 00:00:00
abstract：Objectives:To assess the immediate effect of TEAM® on trauma related knowledge of undergraduate medical students and to highlight the stakeholders' acceptability of TEAM® for trauma training of undergraduate medical students. Methods:Effectiveness of TEAM® course in terms of knowledge gain was assessed using 20-item-M...
journal_title：Pakistan journal of medical sciences
pub_type： 杂志文章
doi：10.12669/pjms.36.6.2588
更新日期：2020-09-01 00:00:00
