New directions for protease inhibitors directed drug discovery.

abstract：:The synthetic peptide of sequence H-Ala-Ser-Thr-Thr-Thr-Asn-Tyr-Thr-OH, termed peptide T, a competitor of the Human Immunodeficiency Virus in the binding to human T cells, and its C-terminal pentapeptide fragment, were studied by 1H-nmr in DMSO solution to determine conformational preferences. The observation of nucle...

journal_title：Biopolymers

authors： Motta A,Picone D,Temussi PA,Marastoni M,Tomatis R

更新日期：1989-01-01 00:00:00

abstract：:Contact lens (CL) wear is a risk factor for development of microbial keratitis, a vision threatening infection of the eye. Adverse events associated with colonization of lenses, especially by the multi-drug resistant and biofilm forming bacterium Pseudomonas aeruginosa remain a major safety issue. Therefore, novel str...

journal_title：Biopolymers

authors： Casciaro B,Dutta D,Loffredo MR,Marcheggiani S,McDermott AM,Willcox MD,Mangoni ML

更新日期：2017-10-31 00:00:00

abstract：:Molecular dynamics (MD) simulations on stem loop 2 of U1 small nuclear RNA and a construct of the U1A protein were carried out to obtain predictions of the structures for the unbound forms in solution and to elucidate dynamical aspects of induced fit upon binding. A crystal structure of the complex between the U1A pro...

journal_title：Biopolymers

authors： Pitici F,Beveridge DL,Baranger AM

更新日期：2002-12-15 00:00:00

abstract：:Gelsolin is an actin-severing protein whose action is initiated by Ca(2+) and inhibited by binding to phosphorylated inositol lipid or phosphoinositides. The regions of gelsolin responsible for phosphoinositide binding are comprised of residues 150-169 (G150-169) and 135-142 (G135-142). The corresponding peptides poss...

journal_title：Biopolymers

authors： Liepiņa I,Czaplewski C,Janmey P,Liwo A

更新日期：2003-01-01 00:00:00

abstract：:Alternating sequences of D and L residues in peptides are directly related to the formation of several kinds of regular helical conformations usually called beta-helices. The major feature of these structures is that they can be associated with the transmembrane ion-conducting channel activity in some natural antibact...

journal_title：Biopolymers

authors： Navarro E,Fenude E,Celda B

更新日期：2004-02-05 00:00:00

abstract：:Structural characterization of G protein-coupled receptors (GPCRs) is hindered by the inherent hydrophobicity, flexibility, and large size of these signaling proteins. Insights into conformational preferences and the three-dimensional (3D) structure of domains of these receptors can be obtained using polypeptide fragm...

journal_title：Biopolymers

authors： Caroccia KE,Estephan R,Cohen LS,Arshava B,Hauser M,Zerbe O,Becker JM,Naider F

更新日期：2011-01-01 00:00:00

abstract：:In N-methyl amino acids, the hydrogen of the N-H group is replaced with a bulky methyl group. While this change is expected to destabilize helical structures, the amount of destabilization is not known. Here the N-methyl group is placed into several positions of the helical peptides, acetyl-WGG(EAAAR)4A-amide and acet...

journal_title：Biopolymers

authors： Chang CF,Zehfus MH

更新日期：1996-01-01 00:00:00

abstract：:Cellulose acetate (DS = 2.45) was extensively investigated by Circular Dichroism (CD) in acetonitrile and dioxane. We found great differences between the CD spectra of a 1 wt % acetonitrile solution and the corresponding dioxane solution of cellulose acetate (CA) indicating that the macromolecules exist in those solut...

journal_title：Biopolymers

authors： Meissner D,Einfeldt J,Schareina T

更新日期：1999-08-01 00:00:00

abstract：:We describe the synthesis of several polyamides that retain the secondary structure of proteins and contain derivatizable side chains. The derivatizable side chain allows for further reaction of the polymer chain (e.g., chain cross-linking or addition of pendant groups). Polymers of alpha-amino acids containing a term...

journal_title：Biopolymers

authors： Guinn RM,Margot AO,Taylor JR,Schumacher M,Clark DS,Blanch HW

更新日期：1995-05-01 00:00:00

abstract：:Piscidin 1 (Pis-1) has a high broad-spectrum activity against bacteria, fungi, and viruses but it also has a moderate hemolytic activities. To improve the antibacterial activity and to reduce toxicity, mutants Pis-1AA (G8A/G13A double mutant) and Pis-1PG (G8P mutant) have been designed based on the crystal structure o...

journal_title：Biopolymers

authors： Yuan T,Zhang X,Hu Z,Wang F,Lei M

更新日期：2012-12-01 00:00:00

abstract：:Second-generation dendrimers have been prepared on solid phase by successive additions of branched polyproline building blocks starting from two different branching units anchored to the solid support. The preparation of Pro-rich building blocks was carried out by stepwise solid-phase synthesis and their iterative add...

journal_title：Biopolymers

authors： Sanclimens G,Crespo L,Giralt E,Royo M,Albericio F

更新日期：2004-01-01 00:00:00

abstract：:The natural amino acids are primarily helix breakers at the low assignment temperatures characteristic of many studies, but recent genomic analyses of thermophilic proteins suggest that at high temperatures, some breakers may become strong helix formers. Moreover, the breaker/former inventory has not been previously c...

journal_title：Biopolymers

authors： Nasr KA,Schubert CR,Török M,Kennedy RJ,Kemp DS

更新日期：2009-05-01 00:00:00

abstract：:We describe a method to determine the magnitude of protein-induced DNA bends relative to a set of standard A tract bends using comparative gel electrophoresis. The DNA bend of interest was that induced by the catabolite activator protein (CAP), the transcriptional activator protein of the lac operon. The set of compar...

journal_title：Biopolymers

authors： Zinkel SS,Crothers DM

更新日期：1990-01-01 00:00:00

abstract：:The equilibrium structural ensemble of a 20-residue polyglutamic acid peptide (E(20)) was studied with FRET, circular dichroism, and molecular dynamics (MD) simulations. A FRET donor, o-aminobenzamide, and acceptor, 3-nitrotyrosine, were introduced at the N- and C-termini, respectively. Circular dichroism, steady stat...

journal_title：Biopolymers

authors： Finke JM,Jennings PA,Lee JC,Onuchic JN,Winkler JR

更新日期：2007-06-15 00:00:00

abstract：:A desire to replicate the structural and functional complexity of proteins with structured, sequence-specific oligomers motivates study of the structural features of water-soluble peptoids (N-substituted glycine oligomers). Understanding the molecular-level details of peptoid self-assembly in water is essential to adv...

journal_title：Biopolymers

authors： Fuller AA,Huber J,Jimenez CJ,Dowell KM,Hough S,Ortega A,McComas KN,Kunkel J,Asuri P

更新日期：2019-04-01 00:00:00

abstract：:Caspases comprise a family of dimeric cysteine proteases that control apoptotic programmed cell death and are therefore critical in both organismal development and disease. Specific inhibition of individual caspases has been repeatedly attempted, but has not yet been attained. Caspase-9 is an upstream or initiator cas...

journal_title：Biopolymers

authors： Huber KL,Ghosh S,Hardy JA

更新日期：2012-01-01 00:00:00

abstract：:The interhelical interfaces have been examined in seven high-resolution globin chains. The profiles of hydrophobic contact, as measured by the residue solvent-accessible area loss upon folding, have been calculated. The seven globins studied differ in their overall loss of solvent-accessible area upon packing of their...

journal_title：Biopolymers

authors： Weaver DL

更新日期：1992-05-01 00:00:00

abstract：:The 32-residue leucine zipper subsequence, called here Jun-lz, associates in benign media to form a parallel two-stranded coiled coil. Studies are reported of its thermal unfolding/folding transition by circular dichroism (CD) on samples of natural isotopic abundance and by both equilibrium and spin inversion transfer...

journal_title：Biopolymers

authors： d'Avignon DA,Bretthorst GL,Holtzer ME,Schwarz KA,Angeletti RH,Mints L,Holtzer A

更新日期：2006-10-15 00:00:00

abstract：:To optimize antigen-antibody reactions, we have synthesized chemically well-defined hydrophilic microspheres. Proteins or haptens were covalently linked to these carriers. When the microsphere conjugates were agglutinated by the corresponding antiserum, the size of the complex artificially increased during the immunol...

journal_title：Biopolymers

authors： Marchand J,Varcin P,Riochet D,Montagne P,Cuilliere ML,Duheille J,Pau B

更新日期：1992-08-01 00:00:00

abstract：:Hexameric insulin is an allosteric protein that undergoes transitions between three conformational states (T(6), T(3)R(3), and R(6)). These allosteric states are stabilized by the binding of ligands to the phenolic pockets and by the coordination of anions to the His B10 metal sites. Raman difference (RD) spectroscopy...

journal_title：Biopolymers

authors： Ferrari D,Diers JR,Bocian DF,Kaarsholm NC,Dunn MF

更新日期：2001-01-01 00:00:00

abstract：:DNA sequences d-TGAGGAAAGAAGGT (a 14-mer) and d-CTCCTTTCTTCC (a 12-mer) are complementary in parallel orientation forming either Donohue (reverse Watson-Crick) base pairing at neutral pH or Hoogsteen base pairing at slightly acidic pH. The structure of the complex formed by dissolving the two strands in equimolar rati...

journal_title：Biopolymers

authors： Bhaumik SR,Chary KV,Govil G,Liu K,Miles HT

更新日期：1997-06-01 00:00:00

abstract：:Cyclic tri-β-peptide having tetrathiafulvalene (TTF) at the side chain was synthesized to prepare a peptide nanotube aligning TTF side chains along the nanotube. The polarized light microscopic observations revealed crystallization of the cyclic peptide by the vapor diffusion method. Fourier-transform infrared and ele...

journal_title：Biopolymers

authors： Uji H,Kim H,Imai T,Mitani S,Sugiyama J,Kimura S

更新日期：2016-05-01 00:00:00

abstract：:An increasing number of RNAs are being discovered that perform their functions by undergoing large changes in conformation in response to a variety of cellular signals, including recognition of proteins and small molecular targets, changes in temperature, and RNA synthesis itself. The measurement of NMR residual dipol...

journal_title：Biopolymers

authors： Getz M,Sun X,Casiano-Negroni A,Zhang Q,Al-Hashimi HM

更新日期：2007-08-05 00:00:00

abstract：:A natural rubber was identified and characterized for the first time in the latex of the perennial Mediterranean shrub Euphorbia characias. Four different methods, i.e., acetone, acetic acid, trichloroacetic acid, and Triton® X-100, followed by successive treatments with cyclohexane/ethanol, were employed to extract t...

journal_title：Biopolymers

authors： Spanò D,Pintus F,Mascia C,Scorciapino MA,Casu M,Floris G,Medda R

更新日期：2012-08-01 00:00:00

abstract：:Infrared spectra of poly(D,L-alanine), poly(L-glutamic acid), poly(L-lysine), silk fibroin, and tropomyosin have been registered for various conformations of the polypeptide chain. Assuming additivity of the main- and side-chain absorption, spectral parameters of amide I and II absorption bands corresponding to alpha-...

journal_title：Biopolymers

authors： Venyaminov SYu,Kalnin NN

更新日期：1990-01-01 00:00:00

abstract：:We have utilized a de novo designed two-stranded α-helical coiled-coil template to display conserved α-helical epitopes from the stem region of hemagglutinin (HA) glycoproteins of influenza A. The immunogens have all the surface-exposed residues of the native α-helix in the native HA protein of interest displayed on t...

journal_title：Biopolymers

authors： Jiang Z,Gera L,Mant CT,Hirsch B,Yan Z,Shortt JA,Pollock DD,Qian Z,Holmes KV,Hodges RS

更新日期：2016-03-01 00:00:00

abstract：:A new family of small plant peptides was recently described and found to be widespread throughout the Millereae and Heliantheae tribes of the sunflower family Asteraceae. These peptides originate from the post-translational processing of unusual seed-storage albumin genes, and have been termed PawS-derived peptides (P...

journal_title：Biopolymers

authors： Franke B,Jayasena AS,Fisher MF,Swedberg JE,Taylor NL,Mylne JS,Rosengren KJ

更新日期：2016-11-01 00:00:00

abstract：:Methods to generate fibers from hydrogels, with control over mechanical properties, fiber diameter, and crystallinity, while retaining cytocompatibility and degradability, would expand options for biomaterials. Here, we exploited features of silk fibroin protein for the formation of tunable silk hydrogel fibers. The b...

journal_title：Biopolymers

authors： Bradner SA,Partlow BP,Cebe P,Omenetto FG,Kaplan DL

更新日期：2017-09-01 00:00:00

abstract：:Serine palmitoyltransferase (SPT) catalyses the first step in the de novo biosynthesis of sphingolipids (SLs). It uses a decarboxylative Claisen-like condensation reaction to couple L-serine with palmitoyl-CoA to generate a long-chain base product, 3-ketodihydrosphingosine. SLs are produced by mammals, plants, yeast, ...

journal_title：Biopolymers

authors： Raman MC,Johnson KA,Clarke DJ,Naismith JH,Campopiano DJ

更新日期：2010-09-01 00:00:00

abstract：:Reactive oxygen species (ROS) have generated a great deal of interest in the clinical field since experimental studies showed the involvement of these species in carcinogenesis. This paper reports the detection of ROS during the decomposition of H2O2 in the presence of samples obtained from tissues of 16 patients with...

journal_title：Biopolymers

authors： Kładny J,Lichszteld K,Kołecki J

更新日期：2004-08-05 00:00:00