Abstract:
:To optimize antigen-antibody reactions, we have synthesized chemically well-defined hydrophilic microspheres. Proteins or haptens were covalently linked to these carriers. When the microsphere conjugates were agglutinated by the corresponding antiserum, the size of the complex artificially increased during the immunological reaction. After optimizing various parameters such as the hydrophilic character, repulsion charges, and amount of antigen coupled to the microspheres, we developed a rapid and sensitive immunoassay based on laser light scattering by the complexes.
journal_name
Biopolymersjournal_title
Biopolymersauthors
Marchand J,Varcin P,Riochet D,Montagne P,Cuilliere ML,Duheille J,Pau Bdoi
10.1002/bip.360320808subject
Has Abstractpub_date
1992-08-01 00:00:00pages
971-80issue
8eissn
0006-3525issn
1097-0282journal_volume
32pub_type
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