A Handy Field-Portable ELISA System for Rapid Onsite Diagnosis of Infectious Diseases.

Abstract:

:Enzyme-linked immunosorbent assays (ELISAs) are considered the gold standard for the detection of various immunological reactions and can be used for the detection of infectious diseases during outbreaks or in the care of individual patients. To be useful in the timely implementation of prevention and control measures against infectious diseases, a diagnostic modality should be rapid, accurate, and affordable. In the current study, we demonstrate the efficiency (90% less time and volume consumption compared with those of a standard 96-well ELISA), detection capability, and ease of operation of a field-portable, battery-operated ELISA system, approximately the size of a cellular phone (12 × 6 × 5.5 cm), in the serological diagnosis of measles and rubella viruses that has the potential for onsite testing such as during disease outbreaks.

journal_name

Jpn J Infect Dis

authors

Singh H,Morioka K,Shimojima M,Van An L,Nakajima H,Hemmi A,Uchiyama K,Loong SK,AbuBakar S,Yang M,Sugamata M

doi

10.7883/yoken.JJID.2015.417

subject

Has Abstract

pub_date

2016-09-21 00:00:00

pages

435-8

issue

5

eissn

1344-6304

issn

1884-2836

journal_volume

69

pub_type

杂志文章
  • The adverse events of influenza A (H1N1) vaccination and its risk factors in healthcare personnel in 18 military healthcare units in Korea.

    abstract::In this study, we characterized adverse events related to influenza A (H1N1) vaccination and studied the factors that influence the occurrence of these events. A total of 4,302 personnel in 18 military healthcare units in Korea received 0.5 ml of inactivated H1N1 vaccine. The study questionnaires were answered by 3,93...

    journal_title:Japanese journal of infectious diseases

    pub_type: 杂志文章

    doi:

    authors: Hwang SM,Choe KW,Cho SH,Yoon SJ,Park DE,Kang JS,Kim MJ,Chun BC,Lee SM

    更新日期:2011-01-01 00:00:00

  • Immune reconstitution inflammatory syndrome among HIV/AIDS patients during highly active antiretroviral therapy in Addis Ababa, Ethiopia.

    abstract::Suppression of viral replication is followed by increases in CD4+ lymphocytes, and this has been shown to result in decreased susceptibility to opportunists after initiation of highly active antiretroviral therapy (HAART). However, clinical aggravations after the initiation of HAART have been thought to be due to the ...

    journal_title:Japanese journal of infectious diseases

    pub_type: 杂志文章

    doi:

    authors: Huruy K,Mulu A,Mengistu G,Shewa-Amare A,Akalu A,Kassu A,Andargie G,Elias D,Torben W

    更新日期:2008-05-01 00:00:00

  • Mechanisms of broad cross-protection provided by influenza virus infection and their application to vaccines.

    abstract::Mice recovered from influenza A virus infection have been shown to be cross-protected against challenge infection with either drift viruses within a subtype (subtype-specific immunity) or different subtype viruses (heterosubtypic immunity). The mechanisms of broad-spectrum of cross-protection could be explained as fol...

    journal_title:Japanese journal of infectious diseases

    pub_type: 杂志文章,评审

    doi:

    authors: Tamura S,Tanimoto T,Kurata T

    更新日期:2005-08-01 00:00:00

  • Seroprevalence of a novel influenza A (H3N2) variant virus in the Japanese population.

    abstract::Antibody distribution to a novel swine-origin influenza A (H3N2) variant virus among different age groups in Japan was determined by hemagglutination-inhibition assay. Japanese children in the age group 1-14 years were at a higher risk than those in older age groups. ...

    journal_title:Japanese journal of infectious diseases

    pub_type: 杂志文章

    doi:10.7883/yoken.66.549

    authors: Kishida N,Imai M,Xu H,Taya K,Fujisaki S,Takashita E,Tashiro M,Odagiri T

    更新日期:2013-01-01 00:00:00

  • Positive Stool Test Results Suggest that the Discharge Standard for COVID-19 Needs Improvement.

    abstract::We report on a hospitalized patient with 2019 novel coronavirus disease whose fecal samples tested negative 22 days after respiratory samples tested negative, highlighting that the duration of viral shedding is longer than that previously expected. Current clinical examinations for treatment and discharge standards ar...

    journal_title:Japanese journal of infectious diseases

    pub_type: 杂志文章

    doi:10.7883/yoken.JJID.2020.265

    authors: Ge R,Chen Z,Liu X,Zhang Q,Zhu G,Xiao Q

    更新日期:2021-01-22 00:00:00

  • Prediction of Contact Residues in Anti-HIV Neutralizing Antibody by Deep Learning.

    abstract::The monoclonal antibody 1C10 targets the V3 loop of HIV-1 and neutralizes a broad range of clade B viruses. However, the mode of interaction between 1C10 and the V3 loop remains unclear because crystallization of 1C10 and the V3 peptide was unsuccessful due to the flexible regions present in both 1C10 and the V3 pepti...

    journal_title:Japanese journal of infectious diseases

    pub_type: 杂志文章

    doi:10.7883/yoken.JJID.2019.496

    authors: Kaku Y,Kuwata T,Gorny MK,Matsushita S

    更新日期:2020-05-22 00:00:00

  • Noncirrhotic portal hypertension associated with didanosine: a case report and literature review.

    abstract::Noncirrhotic portal hypertension (NCPH) has recently been reported as a liver complication in human immunodeficiency virus (HIV)-infected patients and has been found to be associated with exposure to didanosine. Here, we describe the case of an HIV-infected patient with portal hypertension who initially presented w...

    journal_title:Japanese journal of infectious diseases

    pub_type: 杂志文章,评审

    doi:

    authors: Chang HM,Tsai HC,Lee SS,Wann SR,Chen YS

    更新日期:2012-01-01 00:00:00

  • Syphilis seroprevalence among patients attending a sexually transmitted disease clinic in West Bengal, India.

    abstract::Syphilis is a sexually transmitted disease (STD) and is a major public health concern in India. The trend of syphilis infection was studied in attendees aged 15-49 years in an STD clinic in West Bengal from 2004 to 2008. Blood samples were collected from 250 consecutive patients per year (a total of 1,250 samples o...

    journal_title:Japanese journal of infectious diseases

    pub_type: 杂志文章

    doi:

    authors: Maity S,Bhunia SC,Biswas S,Saha MK

    更新日期:2011-01-01 00:00:00

  • Ten-Year Surveillance of Measles Virus from 2007-2016 in Osaka City, Japan.

    abstract::Measles is a highly contagious infection caused by the measles virus (MV). This study performed long-term surveillance in order to survey the prevalence of MV. A total of 417 patients diagnosed with or suspected of having measles were tested for MV between January 2007 and December 2016 in Osaka City, Japan. Reverse t...

    journal_title:Japanese journal of infectious diseases

    pub_type: 杂志文章

    doi:10.7883/yoken.JJID.2017.322

    authors: Kaida A,Iritani N,Kanbayashi D,Yamamoto SP,Hirai Y,Hakui N,Fujimori R,Mori H,Hirokawa H,Ogasawara J,Kubo H

    更新日期:2018-03-22 00:00:00

  • Japanese encephalitis in mainland china.

    abstract::Japanese encephalitis (JE) is a seasonal epidemic disease with a 50-year recorded history in China. Its characteristics can be summarized as follows: (i) it is a seasonal epidemic disease; approximately 90% of cases are recorded in July, August, and September each year. The peak of JE onset is 1 month earlier in South...

    journal_title:Japanese journal of infectious diseases

    pub_type: 杂志文章,评审

    doi:

    authors: Wang H,Li Y,Liang X,Liang G

    更新日期:2009-09-01 00:00:00

  • Mice with Streptozotocin-Induced Hyperglycemia are Susceptible to Invasive Enteric Bacterial Infection.

    abstract::Diabetes mellitus and diarrhea are becoming increasingly burdensome worldwide, particularly in developing countries such as India. Diabetic patients are susceptible to infection with pathogenic bacteria, particularly those causing invasive enteric infections. In this study, we observed changes in the pathophysiologica...

    journal_title:Japanese journal of infectious diseases

    pub_type: 杂志文章

    doi:10.7883/yoken.JJID.2015.418

    authors: Koley H,Ghosh P,Sinha R,Mitra S,Mukherjee P,Howlader DR,Chaki C,Nag D

    更新日期:2017-01-24 00:00:00

  • Prevalence of Providencia Strains among Patients with Diarrhea and in Retail Meats in Thailand.

    abstract::In the present study, we examined the prevalence of Providencia spp. in retail meats and in stool samples from diarrheal patients in Thailand. Providencia-specific (Psp)-PCR amplification was positive for 58% (15/26) of the chicken, 64% (16/25) of the pork, and 68% (17/25) of the beef samples. Subsequently, Providenci...

    journal_title:Japanese journal of infectious diseases

    pub_type: 杂志文章

    doi:10.7883/yoken.JJID.2015.224

    authors: Shima A,Hinenoya A,Samosornsuk W,Samosornsuk S,Mungkornkaew N,Yamasaki S

    更新日期:2016-07-22 00:00:00

  • New rapid screen for the detection of imipenem-resistant Acinetobacter baumanii.

    abstract::A rapid screen was developed for the detection of imipenem-resistant Acinetobacter baumanii (IRAb) following a recent outbreak in the Surgical Intensive Care Unit (SICU) of a hospital in Singapore. Antimicrobial solutions of imipenem ranging from 16 mg/L to 64 mg/L were prepared in-house. Each of the antimicrobial sol...

    journal_title:Japanese journal of infectious diseases

    pub_type: 杂志文章

    doi:

    authors: Chiew YF

    更新日期:2005-02-01 00:00:00

  • Safety and Immunogenicity of the Quadrivalent HPV Vaccine in Japanese Boys: a Phase 3, Open-Label Study.

    abstract::Human papillomavirus (HPV)-associated disease is common among men with HPV infection. A quadrivalent HPV (qHPV) vaccine has demonstrated 85.9% efficacy against HPV6/11/16/18-related, persistent (≥ 6 month) infection in a study of Japanese men aged 16-26 years old. Here, we report the results of an open-label study of ...

    journal_title:Japanese journal of infectious diseases

    pub_type: 杂志文章

    doi:10.7883/yoken.JJID.2018.448

    authors: Murata S,Takeuchi Y,Yamanaka K,Hayakawa J,Yoshida M,Yokokawa R,Wakana A,Sawata M,Tanaka Y

    更新日期:2019-09-19 00:00:00

  • Identification of Group G Streptococcal Isolates from Companion Animals in Japan and Their Antimicrobial Resistance Patterns.

    abstract::In this study, we conducted a species-level identification of group G streptococcal (GGS) isolates from companion animals in Japan and analyzed antimicrobial resistance (AMR) patterns. Strains were isolated from sterile and non-sterile specimens collected from 72 animals with clinical signs or symptoms in April-May, 2...

    journal_title:Japanese journal of infectious diseases

    pub_type: 杂志文章

    doi:10.7883/yoken.JJID.2016.375

    authors: Tsuyuki Y,Kurita G,Murata Y,Goto M,Takahashi T

    更新日期:2017-07-24 00:00:00

  • In vitro activity of linezolid against Mycobacterium tuberculosis strains isoalted from Western Turkey.

    abstract::We investigated the linezolid susceptibility of Mycobacterium tuberculosis strains isolated from a tertiary care hospital in Izmir. A total of 67 M. tuberculosis strains (33 multidrug-resistant [MDR] and 34 non-MDR) were isolated and identified by the Tuberculosis Laboratory, Department of Microbiology and Clinical Mi...

    journal_title:Japanese journal of infectious diseases

    pub_type: 杂志文章

    doi:

    authors: Ermertcan S,Hosgor-Limoncu M,Erac B,Tasli H,Cavusoglu C,Bozkurt H

    更新日期:2009-09-01 00:00:00

  • Molecular characterization of extended-spectrum beta-lactamases produced by clinical isolates of Enterobacter cloacae from a Teaching Hospital in China.

    abstract::Of 59 clinical isolates of Enterobacter cloacae from a teaching hospital in Sichuan, China, 18 isolates were shown to be resistant to oxyimino cephalosporins and aztreonam. Enterobacterial repetitive consensus PCR revealed that these isolates comprised 7 distinct genotypes. The presence of plasmids in the 18 clinical ...

    journal_title:Japanese journal of infectious diseases

    pub_type: 杂志文章

    doi:

    authors: Liu G,Ling BD,Zeng Y,Lin L,Xie YE,Lei J

    更新日期:2008-07-01 00:00:00

  • Seasonal influenza surveillance using prescription data for anti-influenza medications.

    abstract::Surveillance is critical for both early detection and a timely public health response to infectious diseases. Electronic information systems have been widely adopted by pharmacies in Japan. The aim of this study is to evaluate the feasibility of using prescription data for influenza surveillance in Japan to facilitate...

    journal_title:Japanese journal of infectious diseases

    pub_type: 杂志文章

    doi:

    authors: Yoshida M,Matsui T,Ohkusa Y,Kobayashi J,Ohyama T,Sugawara T,Yasui Y,Tachibana T,Okabe N

    更新日期:2009-05-01 00:00:00

  • Stability of JC virus DNA in cerebrospinal fluid specimens preserved with guanidine lysis buffer for quantitative PCR testing.

    abstract::Quantitative PCR testing for JC virus (JCV) DNA in the cerebrospinal fluid (CSF) is one of the diagnostic standards for progressive multifocal leukoencephalopathy (PML). The present study was conducted to examine its reliability using CSF specimens that had been preserved with guanidine lysis buffers in commercial nuc...

    journal_title:Japanese journal of infectious diseases

    pub_type: 杂志文章

    doi:10.7883/yoken.67.307

    authors: Nakamichi K,Lim CK,Saijo M

    更新日期:2014-01-01 00:00:00

  • Dengue virus 4 (DENV-4) re-emerges after 30 years in Brazil: cocirculation of DENV-2, DENV-3, and DENV-4 in Bahia.

    abstract::Dengue fever (DF) is a mosquito-borne viral disease of great concern in tropical and subtropical regions of the world. One important cause of the increase in DF is rapid development and urbanization has led to proliferation of the Aedes aegypti mosquito, the vector responsible for transmission of the illness. Surveill...

    journal_title:Japanese journal of infectious diseases

    pub_type: 杂志文章

    doi:10.7883/yoken.JJID.2014.063

    authors: Campos GS,Pinho AC,Brandão CJ,Bandeira AC,Sardi SI

    更新日期:2015-01-01 00:00:00

  • Reduced incorporation of SARS-CoV spike protein into viral particles due to amino acid substitutions within the receptor binding domain.

    abstract::Cell clone #21 is a long-term producer of the infectious SARS-coronavirus, although the incorporation rate of spike (S) protein into virions is significantly lower. Sequencing analysis of the viral structural proteins revealed four and one amino acid substitutions in the S and membrane (M) proteins, respectively. We d...

    journal_title:Japanese journal of infectious diseases

    pub_type: 杂志文章

    doi:

    authors: Li SM,Li GM,Nakamura S,Ikuta K,Nakaya T

    更新日期:2008-03-01 00:00:00

  • Relationship between MMP Expression and Virulence of Dengue Virus Type-2 in Infected Mosquito and Mammalian Cells.

    abstract::Dengue virus infections are mostly asymptomatic but can produce a mild, self-limiting acute febrile illness, dengue fever, or a life threatening severe illness, dengue hemorrhagic fever. Dengue hemorrhagic fever is associated with increased vascular permeability partly as a result of elevated levels of matrix metallop...

    journal_title:Japanese journal of infectious diseases

    pub_type: 杂志文章

    doi:10.7883/yoken.JJID.2014.323

    authors: Leaungwutiwong P,Kelley JF,Sachair A,Jittmittraphap A,Luplertlop N

    更新日期:2016-01-01 00:00:00

  • HIV/AIDS acquisition and transmission in Bangladesh: turning to the concentrated epidemic.

    abstract::A seventh round behavioral and serological surveillance found that the HIV epidemic had remarkably increased to 7% among intravenous drug users (IDU) in Central Bangladesh, indicating the urgent need to increase prevention. The main purposes of this study were to find out, by collecting data and the necessary informat...

    journal_title:Japanese journal of infectious diseases

    pub_type: 杂志文章

    doi:

    authors: Mondal NI,Takaku H,Ohkusa Y,Sugawara T,Okabe N

    更新日期:2009-03-01 00:00:00

  • Fc gamma receptor IIa polymorphism in patients with brucellosis.

    abstract::Recent evidence suggests that certain Fc gamma receptor alleles are genetic risk factors for infectious diseases. In this study, we evaluated Fc gamma RIIa polymorphism in patients with brucellosis. In a case-control study, the frequency of two alleles and three genotypes for Fc gamma RIIa were measured by PCR in 150 ...

    journal_title:Japanese journal of infectious diseases

    pub_type: 杂志文章

    doi:

    authors: Hashemi SH,Hajilooi M,Mamani M,Jamal-Omidi S

    更新日期:2007-07-01 00:00:00

  • Analysis of Human Parechovirus Genotypes in Yokohama District from 2000 to 2016.

    abstract::Human parechovirus (HPeV) infections in Yokohama City, Japan, were surveyed from 2000 to 2016. The sequence of the VP1 region of HPeVs was used to construct a phylogenetic tree and to reveal the putative amino acid (aa) sequences. Phylogenetic analysis showed the presence of 3 genotypes in Yokohama City: HPeV1 (25 spe...

    journal_title:Japanese journal of infectious diseases

    pub_type: 杂志文章

    doi:10.7883/yoken.JJID.2017.490

    authors: Momoki TS

    更新日期:2018-07-24 00:00:00

  • Characterization of CTX-M-22 and TEM-141 Encoded by a single plasmid from a clinical isolate of Enterobacter cloacae in China.

    abstract::We analyzed the resistance to expanded-spectrum cephalosporins of an Enterobacter cloacae clinical isolate, EC002, by transconjugation, isoelectric-focusing analysis, and cloning experiments. It produced two beta-lactamases with isoelectric point values of 5.4 and 8.7, corresponding to TEM-141, a novel variant of TEM-...

    journal_title:Japanese journal of infectious diseases

    pub_type: 杂志文章

    doi:

    authors: Liu G,Ling BD,Xie YE,Lin L,Zeng Y,Zhang X,Lei J

    更新日期:2007-09-01 00:00:00

  • Loa loa and Mansonella perstans infections in Ijebu north, western Nigeria: a parasitological study.

    abstract::The prevalence and intensity of Loa loa and Mansonella perstans were studied in three villages of the Ijebu north area in Ogun State, western Nigeria. Blood samples were collected by finger-pricking from 373 (181 males, 192 females) subjects with an age range of 4 to 55 years. The blood samples were examined microscop...

    journal_title:Japanese journal of infectious diseases

    pub_type: 杂志文章

    doi:

    authors: Agbolade M,Akinboye DO

    更新日期:2001-06-01 00:00:00

  • Recurrent fatal necrotizing fasciitis due to Streptococcus pyogenes in a child with hereditary sensory and automic neuropathy type IV.

    abstract::Although necrotizing fasciitis (NF) is a rapidly progressive infection, recurrent NF is very rare. Herein we report a rare case of recurrent NF due to Streptococcus pyogenes. A 12-year-old female with hereditary sensory and autonomic neuropathy (HSAN) type IV presented with fever and swelling on her left knee. NF was ...

    journal_title:Japanese journal of infectious diseases

    pub_type: 杂志文章

    doi:

    authors: Kuzdan C,Soysal A,Altinkanat G,Aksu B,Söyletir G,Bakir M

    更新日期:2011-01-01 00:00:00

  • A quantitative in vitro assay for detecting biological activity of endotoxin using rabbit peripheral blood.

    abstract::The pyrogen test or the endotoxin test has been playing a crucial role in detecting endotoxin in parenteral drugs. The current test methods, however, have disadvantages such as requiring a relatively high number of animals or an inadequacy in direct evaluation of in vivo activity. We made an attempt to establish a new...

    journal_title:Japanese journal of infectious diseases

    pub_type: 杂志文章

    doi:

    authors: Ochiai M,Kataoka M,Toyoizumi H,Yamamoto A,Horiuchi Y

    更新日期:2001-08-01 00:00:00

  • Tularemia, a reemerging disease in northwest Turkey: epidemiological investigation and evaluation of treatment responses.

    abstract::An outbreak of tularemia occurred in three provinces in Turkey in February 2004 and reemerged in the same provinces in February 2005. A total of 61 cases, 54 of which were confirmed with the micro-agglutination test, were diagnosed with oropharyngeal tularemia. No culture for Francisella tularensis was attempted, but ...

    journal_title:Japanese journal of infectious diseases

    pub_type: 杂志文章

    doi:

    authors: Celebi G,Baruönü F,Ayoğlu F,Cinar F,Karadenizli A,Uğur MB,Gedikoğlu S

    更新日期:2006-08-01 00:00:00