Abstract:
:A rapid screen was developed for the detection of imipenem-resistant Acinetobacter baumanii (IRAb) following a recent outbreak in the Surgical Intensive Care Unit (SICU) of a hospital in Singapore. Antimicrobial solutions of imipenem ranging from 16 mg/L to 64 mg/L were prepared in-house. Each of the antimicrobial solutions was then incorporated singularly into MacConkey agar plates by two different methods. One of the methods involved preparing MacConkey agar plates in-house and then adding the antimicrobial solution before the agar solidified (AS method). In the second method, 1 ml of the antimicrobial solution was poured onto the surface of the agar plate (LAS method). Fifty hand-nutrient broth washes of medical staff working in the SICU, Medical Intensive Care Unit, Medical Rehabilitation Ward, and Surgical Rehabilitation Ward were inoculated onto the two types of agar media. Two strains of IRAb were isolated from the hands. The LAS plates showed faster bacterial growth of resistant pathogens by about 24 h, and their detection was easier because susceptible bacteria were inhibited by the antimicrobial. The LAS method incorporating imipenem at 32 mg/L is recommended for the rapid screening of resistant pathogens in the routine clinical laboratory.
journal_name
Jpn J Infect Disjournal_title
Japanese journal of infectious diseasesauthors
Chiew YFsubject
Has Abstractpub_date
2005-02-01 00:00:00pages
36-8issue
1eissn
1344-6304issn
1884-2836journal_volume
58pub_type
杂志文章abstract::Forty-five antiretroviral-naive HIV-1 infected patients and 14 healthy controls in North China were enrolled in this study. The frequency of CD4+CD25+Foxp3+ regulatory T cells (Tregs) and levels of expression of CD95, HLA-DR and CD38 in T cells were detected by flow cytometry. We found that the frequency of Tregs was ...
journal_title:Japanese journal of infectious diseases
pub_type: 杂志文章
doi:
更新日期:2008-09-01 00:00:00
abstract::Of 59 clinical isolates of Enterobacter cloacae from a teaching hospital in Sichuan, China, 18 isolates were shown to be resistant to oxyimino cephalosporins and aztreonam. Enterobacterial repetitive consensus PCR revealed that these isolates comprised 7 distinct genotypes. The presence of plasmids in the 18 clinical ...
journal_title:Japanese journal of infectious diseases
pub_type: 杂志文章
doi:
更新日期:2008-07-01 00:00:00
abstract::Melioidosis is an infection caused by Burkholderia pseudomallei that usually involves the respiratory tract. It may manifest as pneumonia, septicemia, or localized infection. We present here a case of melioidosis initially manifesting as a mass over the supraclavicular area and subsequently progressing to necrotizing ...
journal_title:Japanese journal of infectious diseases
pub_type: 杂志文章
doi:
更新日期:2008-03-01 00:00:00
abstract::The findings of this study revealed a 22% prevalence of Streptococcus pneumoniae among healthy children in Japan; the prevalence tended to increase with age. We identified attendance at day-care facilities and the presence of older siblings as the major risk factors associated with pneumococcal carriage. A significant...
journal_title:Japanese journal of infectious diseases
pub_type: 杂志文章
doi:10.7883/yoken.66.22
更新日期:2013-01-01 00:00:00
abstract::The prevalence of Mycoplasma pneumoniae among HIV-positive patients with community-acquired pneumonia (CAP) remains unclear. We investigated 300 HIV-positive adults (200 with CAP and 100 with no respiratory illness) and 75 HIV-negative adults with CAP for the prevalence of respiratory pathogens using culture and serol...
journal_title:Japanese journal of infectious diseases
pub_type: 杂志文章
doi:
更新日期:2007-11-01 00:00:00
abstract::Stigma, considered a social disease, is more apparent in developing societies which are driven by various social affairs, and influences adherence to treatment. The aim of the present study was to examine levels of social stigma related to tuberculosis (TB) in sociodemographic context and identify the effects of socio...
journal_title:Japanese journal of infectious diseases
pub_type: 杂志文章
doi:10.7883/yoken.JJID.2014.522
更新日期:2015-01-01 00:00:00
abstract::Variable incidence rates of rotavirus gastroenteritis hospitalizations have been reported in Japan. However, it is not known whether the observed regional differences were due to the real difference in the occurrence of severe disease or other causes. This study aimed to determine the incidence rates of rotavirus hosp...
journal_title:Japanese journal of infectious diseases
pub_type: 杂志文章
doi:10.7883/yoken.JJID.2016.052
更新日期:2017-03-24 00:00:00
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journal_title:Japanese journal of infectious diseases
pub_type: 杂志文章
doi:10.7883/yoken.66.69
更新日期:2013-01-01 00:00:00
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journal_title:Japanese journal of infectious diseases
pub_type: 杂志文章
doi:10.7883/yoken.JJID.2020.264
更新日期:2021-01-22 00:00:00
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journal_title:Japanese journal of infectious diseases
pub_type: 杂志文章
doi:10.7883/yoken.JJID.2020.589
更新日期:2020-10-30 00:00:00
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journal_title:Japanese journal of infectious diseases
pub_type: 杂志文章
doi:
更新日期:2008-05-01 00:00:00
abstract::A growing body of evidence continues to emerge implicating the role of myeloperoxidase (MPO) and its oxidant products in the promotion of atherogenesis. A major mechanism by which MPO impacts the arterial wall is through its modification of net cellular cholesterol flux. MPO promotes lipid peroxidation and conversion ...
journal_title:Japanese journal of infectious diseases
pub_type: 杂志文章,评审
doi:
更新日期:2004-10-01 00:00:00
abstract::We evaluated the seroprevalence of vaccine-preventable infectious diseases among Japanese healthcare students to create immunization guidelines. Between 2007 and 2012, a total of 1746 Japanese medical, nursing, and paramedical students were serologically screened for measles, mumps, rubella, varicella, and hepatitis B...
journal_title:Japanese journal of infectious diseases
pub_type: 杂志文章
doi:10.7883/yoken.66.411
更新日期:2013-01-01 00:00:00
abstract::Syphilis is a sexually transmitted disease (STD) and is a major public health concern in India. The trend of syphilis infection was studied in attendees aged 15-49 years in an STD clinic in West Bengal from 2004 to 2008. Blood samples were collected from 250 consecutive patients per year (a total of 1,250 samples o...
journal_title:Japanese journal of infectious diseases
pub_type: 杂志文章
doi:
更新日期:2011-01-01 00:00:00
abstract::Following the 2018 rubella outbreak in Japan, this study aimed to assess rubella prevention measures based on the vaccination and immunization status of pregnant women in Japan. Our cohort study involved 3 local core hospitals in Yokohama City, and a total of 666 pregnant women were recruited between June 2018 and Sep...
journal_title:Japanese journal of infectious diseases
pub_type: 杂志文章
doi:10.7883/yoken.JJID.2020.762
更新日期:2020-12-25 00:00:00
abstract::Meropenem-susceptible and -resistant Aeromonas dhakensis isolates from blood cultures of a fatal case of septicemia were analyzed. The two isolates were homologous and gene expression of metallo-β-lactamase in the resistant strain was upregulated. Physicians should be aware of the possibility of the induction of carba...
journal_title:Japanese journal of infectious diseases
pub_type: 杂志文章
doi:10.7883/yoken.JJID.2015.098
更新日期:2016-09-21 00:00:00
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journal_title:Japanese journal of infectious diseases
pub_type: 历史文章,杂志文章
doi:10.7883/yoken.JJID.2017.087
更新日期:2017-11-22 00:00:00
abstract::Two multidrug-resistant Pseudomonas aeruginosa strains harboring blaVIM were isolated from a hospital in Egypt and were indistinguishable by pulsed-field gel electrophoresis. Sequence analysis revealed a novel VIM β-lactamase, VIM-28. Additional sequencing of integron revealed that the blaVIM-28 gene cassette had an u...
journal_title:Japanese journal of infectious diseases
pub_type: 杂志文章
doi:
更新日期:2014-01-01 00:00:00
abstract::We aimed to evaluate the treatment outcome of pulmonary tuberculosis patients and factors affecting treatment outcomes. We analyzed the records of 586 pulmonary tuberculosis patients who were older than 15 years followed between January 1999 and December 2004. Of these patients, 76.1% were smear-positive for tuberculo...
journal_title:Japanese journal of infectious diseases
pub_type: 杂志文章
doi:
更新日期:2008-01-01 00:00:00
abstract::Understanding the local factors influencing the transmission of communicable diseases is important to minimize social damage. The aim of this study was to investigate local factors influencing seasonal influenza epidemics in Aomori prefecture consisting of 6 regions, i.e., Seihoku, Chunan, and Tosei on the west side, ...
journal_title:Japanese journal of infectious diseases
pub_type: 杂志文章
doi:10.7883/yoken.JJID.2015.500
更新日期:2016-11-22 00:00:00
abstract::Feral raccoons captured in Hokkaido, Japan were examined for the presence of rabies virus neutralizing antibody (VNA). Between 2000 to 2002, 741 serum samples were collected and then subjected to VNA titer determination by rapid fluorescent focus inhibition test (RFFIT). Sera showing RFFIT titers of > or =1:25 have be...
journal_title:Japanese journal of infectious diseases
pub_type: 杂志文章
doi:
更新日期:2004-06-01 00:00:00
abstract::The major objective of this study was to deliver vital statistics related to cholera to health authorities so as to aid in their attempt to prioritize communicable diseases in Nepal. A laboratory-based surveillance was conducted from mid-June 2008 to mid-January 2009 at the National Public Health Laboratory, Nepal. Di...
journal_title:Japanese journal of infectious diseases
pub_type: 杂志文章
doi:
更新日期:2010-05-01 00:00:00
abstract::A molecular epidemiological study of rotavirus (RV) and norovirus (NoV) infections was carried out in Nha Trang city in Vietnam between December 2005 and June 2006. RV and NoV were detected in 87 (47.5%) and 12 (6.6%) of the 183 fecal specimens from children hospitalized with acute gastroenteritis, respectively. The m...
journal_title:Japanese journal of infectious diseases
pub_type: 杂志文章
doi:
更新日期:2010-11-01 00:00:00
abstract::The objective of this study was to characterize the off-seasonal influenza virus A subtype H3N2, which caused an outbreak in an elderly hospital in Niigata, Japan. Virus isolates were subtyped by the hemagglutination-inhibition test and screened for antiviral drug sensitivity by real-time PCR using cycling probe techn...
journal_title:Japanese journal of infectious diseases
pub_type: 杂志文章
doi:
更新日期:2011-01-01 00:00:00
abstract::The efficacy of polyene macrolides to treat experimental Trichosporon bloodstream infection was evaluated by histopathological examination and viable cell counts in the kidneys of infected mice. Viable cell counts on the 5th day after infection confirmed that liposomal amphotericin B (L-AMB) is a more effective treatm...
journal_title:Japanese journal of infectious diseases
pub_type: 杂志文章
doi:10.7883/yoken.66.133
更新日期:2013-01-01 00:00:00
abstract::Chryseobacterium meningosepticum is an uncommon pathogen causing adult bacterial meningitis. Herein, we report the case history of one 21-year-old woman with this uncommon central nervous system infection. A diagnosis of adult C. meningosepticum meningitis can only be confirmed by a positive cerebrospinal fluid (CSF) ...
journal_title:Japanese journal of infectious diseases
pub_type: 杂志文章,评审
doi:
更新日期:2004-10-01 00:00:00
abstract::The prevalence and intensity of Loa loa and Mansonella perstans were studied in three villages of the Ijebu north area in Ogun State, western Nigeria. Blood samples were collected by finger-pricking from 373 (181 males, 192 females) subjects with an age range of 4 to 55 years. The blood samples were examined microscop...
journal_title:Japanese journal of infectious diseases
pub_type: 杂志文章
doi:
更新日期:2001-06-01 00:00:00
abstract::Curtailing the observation of mice challenged with tetanus toxin in potency test of tetanus vaccine would reduce the agony of mice from spastic paralysis. From the viewpoint of animal welfare, we investigated the feasibility this measure. The potencies of 85 lots of vaccine obtained on the 4th day after challenge were...
journal_title:Japanese journal of infectious diseases
pub_type: 杂志文章
doi:
更新日期:2004-12-01 00:00:00
abstract::This study was aimed at determining the molecular epidemiology of rabies virus (RABV) circulating in Vietnam. Intra vitam samples (saliva and cerebrospinal fluid) were collected from 31 patients who were believed to have rabies and were admitted to hospitals in northern provinces of Vietnam. Brain samples were collect...
journal_title:Japanese journal of infectious diseases
pub_type: 杂志文章
doi:
更新日期:2011-01-01 00:00:00
abstract::This study aimed to determine the levels of the macrolides-lincosamides-streptogramins B (MLS(B)) resistance phenotype of Staphylococcus aureus and coagulase-negative staphylococci (CNS) isolates from clinical samples. A total of 521 strains of staphylococci, comprising 230 S. aureus and 291 CNS isolates from various ...
journal_title:Japanese journal of infectious diseases
pub_type: 杂志文章
doi:
更新日期:2005-04-01 00:00:00