Abstract：
:The objective of this study was to characterize the off-seasonal influenza virus A subtype H3N2, which caused an outbreak in an elderly hospital in Niigata, Japan. Virus isolates were subtyped by the hemagglutination-inhibition test and screened for antiviral drug sensitivity by real-time PCR using cycling probe technology the and 50% inhibitory concentration (IC(50)) method. Whole genome sequencing was performed in order to determine the phylogeny of the outbreak virus. Seven virus isolates were analyzed in this study, and the results showed that all belonged to the influenza virus A (H3N2). These viruses exhibited the S31N mutation in M2, which confers resistance to amantadine. The results of the IC(50) analysis showed that these viruses were sensitive to both oseltamivir and zanamivir. Whole genome analysis revealed that the virus was similar to the A/Perth/16/2009 strain and that it is a triple reassortant virus with a 3+3+2 pattern of segment recombination.
journal_name
Jpn J Infect Disjournal_title
Japanese journal of infectious diseasesauthors
Dapat C,Suzuki Y,Kon M,Tamura T,Saito R,Dapat IC,Yamazaki O,Odagiri T,Fujisaki S,Suzuki Hsubject
Has Abstractpub_date
2011-01-01 00:00:00pages
237-41issue
3eissn
1344-6304issn
1884-2836journal_volume
64pub_type
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journal_title：Japanese journal of infectious diseases
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更新日期：2003-08-01 00:00:00
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doi：
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pub_type： 杂志文章,多中心研究
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