Abstract:
:Quantitative PCR testing for JC virus (JCV) DNA in the cerebrospinal fluid (CSF) is one of the diagnostic standards for progressive multifocal leukoencephalopathy (PML). The present study was conducted to examine its reliability using CSF specimens that had been preserved with guanidine lysis buffers in commercial nucleic acid extraction kits under different conditions. When CSFs were mixed with guanidine buffers, JCV DNA levels were not statistically reduced even after storage for 1 month at room temperature or for 3 months at -80℃, compared with the control samples. In addition, the JCV DNA level was not decreased in a mixture of CSF and guanidine thiocyanate buffer incubated for 3 days at 56℃. These data suggest that CSF specimens mixed with commercial guanidine buffers can be stored without refrigeration, more safely handled, and directly subjected to JCV DNA testing for PML.
journal_name
Jpn J Infect Disjournal_title
Japanese journal of infectious diseasesauthors
Nakamichi K,Lim CK,Saijo Mdoi
10.7883/yoken.67.307subject
Has Abstractpub_date
2014-01-01 00:00:00pages
307-10issue
4eissn
1344-6304issn
1884-2836pii
DN/JST.JSTAGE/yoken/67.307journal_volume
67pub_type
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pub_type: 杂志文章,多中心研究
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