Abstract：
PURPOSE:The pathophysiology of Hirschsprung's disease (HSCR) is not entirely understood. There is no clear explanation for the occurrence of the spastic or tonically contracted aganglionic segment of bowel. Kv11.1 (hERG) channels play a critical role in the regulation of the resting membrane potential as well as affecting either the force or frequency of contraction of smooth muscles. We designed this study to investigate the expression and distribution of hERG channels in the normal colon and the colon of patients with HSCR. METHODS:We investigated hERG protein expression in both the ganglionic and aganglionic regions of HSCR patients (n = 10) versus normal control colon (n = 10). Protein distribution was assessed using immunofluorescence and confocal microscopy. Gene and protein expressions were quantified using real-time polymerase chain reaction, western blot analysis and densitometry. RESULTS:Confocal microscopy of the normal colon revealed strong hERG channel expression in interstitial cells of Cajal, platelet-derived growth factor-alpha receptor- (PDGFRα(+)) positive cells and enteric neurons. hERG expression was markedly decreased in aganglionic bowel, whereas colonic hERG gene expression levels were significantly decreased in aganglionic compared to ganglionic bowel and controls (p < 0.05). Western blotting revealed decreased colonic hERG protein expression in aganglionic HSCR specimens compared to controls. CONCLUSIONS:We demonstrate, for the first time, the expression and distribution of hERG channels in the human colon. The decreased expression of hERG in the aganglionic colon may be responsible for the increased tone in the aganglionic narrow spastic segment of bowel.
journal_name
Pediatr Surg Intjournal_title
Pediatric surgery internationalauthors
Tomuschat C,O'Donnell AM,Coyle D,Puri Pdoi
10.1007/s00383-015-3807-8subject
Has Abstractpub_date
2016-01-01 00:00:00pages
9-16issue
1eissn
0179-0358issn
1437-9813pii
10.1007/s00383-015-3807-8journal_volume
32pub_type
杂志文章abstract：:An isolated bowel segment (IBS) is a viable loop of bowel that is completely free of its mesenteric attachments. In this study, omentoenteropexy (OEP) was performed in 5-cm-long IBS of rats in order to provide neovascularization. Thirty-two rats were divided into four groups. After OEP, mesenteric division of the IBSs...
journal_title：Pediatric surgery international
pub_type： 杂志文章
doi：10.1007/BF01076940
更新日期：1997-07-01 00:00:00
abstract：:To evaluate the efficacy of ultrasound (US) guided percutaneous sclerotherapy in the pediatric population, 14 hydatid liver cysts (HLC) in eight male patients whose ages ranged between 6 and 16 years (mean 9.9 years) were treated. The maximum diameter was 110 mm. Albendazole was administered orally to all patients for...
journal_title：Pediatric surgery international
pub_type： 杂志文章
doi：10.1007/s003830000356
更新日期：2000-01-01 00:00:00
abstract：:The presentation of congenital diaphragmatic hernia (CDH) at birth may fall outside the typical features (cyanosis, tachypnea and respiratory failure), manifesting, instead, also with others pictures that make the diagnosis difficult or even impossible. We report a case of CDH presenting as a pneumothorax and a perfor...
journal_title：Pediatric surgery international
pub_type： 杂志文章,评审
doi：10.1007/s00383-007-2000-0
更新日期：2008-03-01 00:00:00
abstract：PURPOSE:Adnexal torsion is a gynecologic emergency, requiring intervention for tissue preservation. At our institution, torsion is managed by pediatric surgeons or gynecologists. We evaluated differences between specialties to streamline evaluation for children with gynecological emergencies, develop a clinical pathway...
journal_title：Pediatric surgery international
pub_type： 杂志文章
doi：10.1007/s00383-020-04782-1
更新日期：2021-01-01 00:00:00
abstract：:Numerous case reports in the literature have suggested a causal link between beta-haemolytic streptococcal infection and delayed-onset right-sided diaphragmatic hernia/eventration in the perinatal age group. We report a child with antenatally documented right diaphragmatic eventration 4 weeks before birth who subseque...
journal_title：Pediatric surgery international
pub_type： 杂志文章
doi：
更新日期：1997-03-21 00:00:00
abstract：PURPOSE:This study aimed to evaluate the mid-term outcomes of single-stage modified anterior sagittal anorectoplasty (ASARP) for anorectal malformation with vestibular fistula. METHOD:Twenty-six patients with congenital imperforate anus and vestibular fistula underwent single-stage modified sphincter-saving ASARP betw...
journal_title：Pediatric surgery international
pub_type： 杂志文章
doi：10.1007/s00383-015-3749-1
更新日期：2015-09-01 00:00:00
abstract：:We present the first two cases of acute neonatal appendicitis operated on through the laparoscopic approach. Acute neonatal appendicitis is uncommon and rarely considered by clinicians when assessing the neonatal acute abdomen. Our two cases demonstrate the potential value of diagnostic laparoscopy in the acute neonat...
journal_title：Pediatric surgery international
pub_type： 杂志文章
doi：10.1007/s00383-011-2972-7
更新日期：2011-11-01 00:00:00
abstract：PURPOSE:Primary spontaneous pneumothorax (PSP) is managed in accordance with the adult British Thoracic Society (BTS) guidelines due to lack of paediatric evidence and consensus. We aim to highlight the differences and provide a best practice surgical management strategy for PSP based on experience of two major paediat...
journal_title：Pediatric surgery international
pub_type： 杂志文章
doi：10.1007/s00383-015-3787-8
更新日期：2015-12-01 00:00:00
abstract：:Hirschsprung's disease (HD) in Down's syndrome (DS) patients is stated to have a worse outcome than HD alone. In our study we reviewed the immediate and long-term outcomes of these children and questioned whether DS should influence the operative management. Data were collected on all children with HD (including total...
journal_title：Pediatric surgery international
pub_type： 杂志文章
doi：10.1007/s00383-005-1617-0
更新日期：2006-02-01 00:00:00
abstract：:Negative pressure wound therapy (NPWT) is an accepted form of treatment in managing complex wounds in adults and children. It is not as widely used in the neonatal population due to limited clinical experience. We describe the application of the RENASYS™ system (Smith and Nephew, UK) in delivering NPWT to four neonate...
journal_title：Pediatric surgery international
pub_type： 杂志文章
doi：10.1007/s00383-011-2868-6
更新日期：2011-08-01 00:00:00
abstract：:Juvenile papillomatosis (JP) is a rare benign, proliferative breast tumor in children. We observed a 9-year-old girl with a large soft-tissue mass at the lower outer quadrant of the left breast. Ultrasonography showed an ill-defined, inhomogeneous mass with numerous small, hypoechoic areas. The tumor was completely ex...
journal_title：Pediatric surgery international
pub_type： 杂志文章
doi：10.1007/s003830000462
更新日期：2001-03-01 00:00:00
abstract：:Oesophageal atresia (OA) is often associated with anomalies of other systems. The genetic contribution to the formation of the VACTERL association is not clear. The objective of this study was to evaluate the incidence of associated anomalies in two different racial populations. The associated anomalies in neonates wi...
journal_title：Pediatric surgery international
pub_type： 杂志文章
doi：10.1007/s003830100692
更新日期：2002-05-01 00:00:00
abstract：:The successful use of a combination of "patch, drain, and wait" (PDW) and home total parenteral nutrition (TPN) in the management of a case of acute, catastrophic midgut volvulus in a 2-year-11-month-old boy with near-total ischemia/necrosis of his small intestine is reported. The PDW approach to the highly effective ...
journal_title：Pediatric surgery international
pub_type： 杂志文章
doi：
更新日期：1997-03-21 00:00:00
abstract：PURPOSE:The aim of this study was to determine the incidence of gastroschisis-related complications (GRCs) after closure of the abdominal wall defect, with a focus on frequency, type and timing of required surgical interventions, and to identify the impact of these on further outcome. METHODS:All gastroschisis patient...
journal_title：Pediatric surgery international
pub_type： 杂志文章
doi：10.1007/s00383-014-3500-3
更新日期：2014-06-01 00:00:00
abstract：:Africa faces a triple burden of disease; HIV, tuberculosis and malaria. Despite this, its population is expected to double over the next 45 years. There is a dire shortage of medical specialists and many countries lack suitably qualified doctors to train medical specialists. Videoconferencing offers the opportunity to...
journal_title：Pediatric surgery international
pub_type： 杂志文章
doi：10.1007/s00383-007-2085-5
更新日期：2008-02-01 00:00:00
abstract：PURPOSE:To date, no detailed study of the changes in the image-defined risk factors (IDRFs) after neoadjuvant chemotherapy has been performed. The aim of this study was to investigate the effect of chemotherapy on IDRFs for stage L2 neuroblastomas. METHODS:Fifteen stage L2 patients treated by neoadjuvant chemotherapy ...
journal_title：Pediatric surgery international
pub_type： 杂志文章
doi：10.1007/s00383-016-3858-5
更新日期：2016-03-01 00:00:00
abstract：:Beckwith-Wiedemann Syndrome (BWS) is associated with somatic overgrowth and a high risk for embryonal tumors, including hepatoblastoma, a highly malignant liver tumor of childhood. Focal nodular hyperplasia (FNH), on the other hand, is a benign tumor of the liver that is uncommon in childhood. Herein we describe a cas...
journal_title：Pediatric surgery international
pub_type： 杂志文章
doi：10.1007/s00383-005-1469-7
更新日期：2005-09-01 00:00:00
abstract：PURPOSE:Bone morphogenetic proteins (BMPs) are a group of growth factors that are implicated in intestinal growth, morphogenesis, differentiation, and homeostasis. The role of the BMP signaling cascade in stimulation of cell proliferation after massive small bowel resection is unknown. The purpose of this study was to ...
journal_title：Pediatric surgery international
pub_type： 杂志文章
doi：10.1007/s00383-015-3829-2
更新日期：2016-02-01 00:00:00
abstract：:New development in the vascular network is a significant process for the proliferation, as well as metastatic expand, of cancer cells that depends on a sufficient provider of oxygen and nutrients and the removal of waste products. New blood and lymphatic vessels form via step called angiogenesis and lymphangiogenesis....
journal_title：Pediatric surgery international
pub_type： 杂志文章
doi：10.1007/s00383-013-3398-1
更新日期：2013-12-01 00:00:00
abstract：:The case of a 9-year-old girl with a Mycobacterium tuberculosis inflammatory myofibroblastic tumor (IMT) of the left lobe of the liver is reported. The tumor was surgically excised and had histological features diagnostic of IMT, a positive Ziehl-Nielsen staining for acid-fast bacilli and a positive polymerase chain r...
journal_title：Pediatric surgery international
pub_type： 杂志文章
doi：10.1007/s00383-009-2361-7
更新日期：2009-05-01 00:00:00
abstract：:A modified technique of laparoscopic pyloromyotomy was used to treat infantile hypertrophic pyloric stenosis. Introducing a 5-mm periumbilical port for visualization, two stab wounds are made on either side laterally to directly insert 2.7-mm instruments for manipulation. From the left, the stomach is grasped - not th...
journal_title：Pediatric surgery international
pub_type： 杂志文章
doi：10.1007/s003830050307
更新日期：1998-04-01 00:00:00
abstract：PURPOSE:In 2006, The Japanese Society of Pediatric Endoscopic Surgeons devised a plan to develop a pediatric endoscopic surgical skill qualification (ESSQ) system. This system is controlled by The Japan Society for Endoscopic Surgery. The standard requirement for skills qualification is the ability of each applicant to...
journal_title：Pediatric surgery international
pub_type： 杂志文章,随机对照试验
doi：10.1007/s00383-011-2871-y
更新日期：2011-07-01 00:00:00
abstract：INTRODUCTION:No protocol exists for prophylaxis or therapeutic management of peripherally inserted central catheter (PICC) related deep venous thrombosis (DVT) in children. Currently, very few patients are provided prophylaxis for DVT. In this study, we analyzed our current practice of PICC placement to identify the fr...
journal_title：Pediatric surgery international
pub_type： 杂志文章
doi：10.1007/s00383-013-3259-y
更新日期：2013-05-01 00:00:00
abstract：PURPOSE:The purpose of this study was to review the clinical characteristics and prognosis of children with adrenocortical tumors (ACT). METHODS:We retrospectively reviewed the medical records of 28 patients with ACT at our hospital between March 2010 and March 2017. RESULTS:The main clinical presentations were sexua...
journal_title：Pediatric surgery international
pub_type： 杂志文章
doi：10.1007/s00383-018-4409-z
更新日期：2019-03-01 00:00:00
abstract：:The advances in science have resulted in the emergence of nanotechnology, which deals with the design and use of tools and devices of size 1-100 nm. The application of nanotechnologies to medicine is thus termed nanomedicine. Significant research has been focused on this new and exciting field and this review article ...
journal_title：Pediatric surgery international
pub_type： 杂志文章,评审
doi：10.1007/s00383-012-3162-y
更新日期：2012-10-01 00:00:00
abstract：:Malignant rhabdoid tumor (MRT) of the kidney is a rare pediatric tumor characterized by its aggressive nature and chemoresistance. Our patient had MRT of the right kidney with tumor thrombus in the renal vein, inferior vena cava, and right atrium. He developed transfusion-resistant hematuria. This was successfully con...
journal_title：Pediatric surgery international
pub_type： 杂志文章
doi：10.1007/s00383-013-3260-5
更新日期：2013-05-01 00:00:00
abstract：OBJECTIVE:The objective of this study is to analyze the effect of exogenous glial cell-derived neurotrophic factor (GDNF) in the development of the enteric nervous system (ENS) in the rectal end of fetal rats. MATERIALS AND METHODS:Thirty pregnant Sprague-Dawley rats were categorized randomly into three groups: ethyle...
journal_title：Pediatric surgery international
pub_type： 杂志文章
doi：10.1007/s00383-012-3251-y
更新日期：2013-04-01 00:00:00
abstract：:Manual reduction with the index finger (modified Hutchinson's maneuver) enabling simple and safe manual reduction of infantile intussusception is described. The procedure was used in four patients, two boys and two girls, ranging in age from 2 to 15 months, as greater than normal compression was necessary and/or seros...
journal_title：Pediatric surgery international
pub_type： 杂志文章
doi：10.1007/s003830050301
更新日期：1998-03-01 00:00:00
abstract：:Modern plastic and reconstructive surgery continues to significantly improve the functional and aesthetic post-burn sequelae in children. It has become a major factor in restoring mobility, aesthetic appearance, self-esteem, and quality of life. The entire spectrum of plastic reconstructive procedures should be availa...
journal_title：Pediatric surgery international
pub_type： 杂志文章,评审
doi：10.1007/BF01076930
更新日期：1997-07-01 00:00:00
abstract：:Controversy exists on the optimal age for elective resection of asymptomatic congenital pulmonary airway malformation. Current recommendations vary widely, highlighting the overall lack of consensus. A systematic search of Embase, MEDLINE, CINAL, and CENTRAL was conducted in January 2016. Identified citations were scr...
journal_title：Pediatric surgery international
pub_type： 杂志文章,meta分析,评审
doi：10.1007/s00383-017-4079-2
更新日期：2017-06-01 00:00:00
