Abstract:
OBJECTIVE:The objective of this study is to analyze the effect of exogenous glial cell-derived neurotrophic factor (GDNF) in the development of the enteric nervous system (ENS) in the rectal end of fetal rats. MATERIALS AND METHODS:Thirty pregnant Sprague-Dawley rats were categorized randomly into three groups: ethylene thiourea (ETU), ETU + GDNF, and control. On day 10 of gestation, ETU was injected via a gastric tube in the ETU group and ETU + GDNF group. On day 11 of gestation, GDNF was administered through the tail vein in the ETU + GDNF group. On day 20 of gestation, fetal rats were harvested by cesarean section. The prevalence of anorectal malformations (ARMs) in the fetal rats was observed. GDNF expression in the rectal end of fetal rats was detected by immunohistochemical and Western blotting analyses. RESULTS:The prevalence of ARMs in the ETU group and ETU + GDNF group was 51.4 and 52.5 %, respectively, but the difference between the two groups was not significant (P > 0.05). In the rectal end of fetal rats with an anus, GDNF expressions in the three groups were not significantly different (P > 0.05). In the rectal end of fetal rats without an anus: GDNF expression in the ETU + GDNF group was significantly higher than that in the ETU group (P = 0.036); GDNF expression in the rectal end of fetal rats without an anus from the ETU group and ETU + GDNF group was significantly lower than that of fetal rats with an anus (ETU group P = 0.001; ETU + GDNF group P = 0.028). There was a significant difference in the gray level ratio of GDNF and actin between the ETU group and ETU + GDNF group (P < 0.0001), and the expression in the ETU + GDNF group was significantly up-regulated. CONCLUSION:GDNF could not totally prevent the occurrence of ETU-induced ARMs, but it up-regulated expression of the GDNF gene in the wall of the rectal end, thereby promoting the growth of a hypogenetic ENS.
journal_name
Pediatr Surg Intjournal_title
Pediatric surgery internationalauthors
Huang Y,Zheng Sdoi
10.1007/s00383-012-3251-ysubject
Has Abstractpub_date
2013-04-01 00:00:00pages
375-9issue
4eissn
0179-0358issn
1437-9813journal_volume
29pub_type
杂志文章abstract::The aim of this study is to evaluate the safety and efficacy of the redo transanal endorectal pull-through (TEPT) for patients with persistent symptoms after pull-through for Hirschsprung's disease (HD). Seven children were included in the study, their ages ranged from 2.5 to 6 years (four males and three females). Th...
journal_title:Pediatric surgery international
pub_type: 杂志文章
doi:10.1007/s00383-006-1837-y
更新日期:2007-02-01 00:00:00
abstract:PURPOSE:For International Neuroblastoma Staging System (INSS) stages III or IV neuroblastoma (intermediate or high risk), complete excision of the primary tumor is not always feasible. Most current studies on the treatment outcome of these patients have reported on the complete excision status. The aim of this study is...
journal_title:Pediatric surgery international
pub_type: 杂志文章
doi:10.1007/s00383-009-2417-8
更新日期:2009-09-01 00:00:00
abstract::This study describes a subset of patients with posterior urethral valves (PUV) who presented late in childhood. The objective was to identify factors that lead to back-pressure effects on the upper tracts, which persist in spite of adequate valve ablation in some patients, and seek factors that may preserve the upper ...
journal_title:Pediatric surgery international
pub_type: 杂志文章
doi:10.1007/s003830050384
更新日期:1998-09-01 00:00:00
abstract:PURPOSE:We have retrospectively evaluated all patients who underwent endoscopic puncture (EP) of ureterocele over the last 26 years with special reference to the need in the second intervention and disease-free status. METHODS:78 (69%) of the 112 patients following EP and completed follow-up were included. 51 (65%) we...
journal_title:Pediatric surgery international
pub_type: 杂志文章
doi:10.1007/s00383-018-4258-9
更新日期:2018-05-01 00:00:00
abstract:PURPOSE:The aim of this study was to determine if there is a difference between complications for totally implantable central venous catheters (ports) and tunnelled external central venous catheters (external CVCs) that result in early removal of the central venous catheter (CVC) in children and adolescents with acute ...
journal_title:Pediatric surgery international
pub_type: 杂志文章
doi:10.1007/s00383-012-3213-4
更新日期:2012-12-01 00:00:00
abstract:PURPOSE:Radiologically inserted gastrojejunal tubes (RGJ) and surgical jejunostomy (SJ) are established modes of jejunal feeding. The aim of the study is to review nutritional outcomes, complications and the practical consideration to enable patients and carers to make informed choice. METHODS:Retrospective review of ...
journal_title:Pediatric surgery international
pub_type: 杂志文章
doi:10.1007/s00383-018-4303-8
更新日期:2018-09-01 00:00:00
abstract:PURPOSE:To describe the management of cavoatrial extension of nephroblastoma in a developing country. PATIENTS AND METHODS:Of 406 consecutive children with Wilms tumour, 40 (10%) presented with tumour extension into the inferior vena cava (IVC) (30), right atrium or right ventricle and form the inception cohort. Of th...
journal_title:Pediatric surgery international
pub_type: 杂志文章
doi:10.1007/s00383-010-2667-5
更新日期:2010-12-01 00:00:00
abstract:INTRODUCTION:Objectively evaluating the lack of bowel control (fecal incontinence) continues to be a challenge. Many have attempted to measure the severity of fecal incontinence and to evaluate its impact on the quality of life by developing standardized scoring systems. Some of these systems have been validated but no...
journal_title:Pediatric surgery international
pub_type: 杂志文章,评审
doi:10.1007/s00383-016-3909-y
更新日期:2016-08-01 00:00:00
abstract:PURPOSE:To show whether Alvarado, Eskelinen, Lintula and Ohmann scoring systems have predictive values in diagnosing acute appendicitis in children. METHODS:Sixty patients with suspected acute appendicitis were prospectively evaluated. Alvarado, Eskelinen, Lintula and Ohmann scores were calculated separately for each ...
journal_title:Pediatric surgery international
pub_type: 杂志文章
doi:10.1007/s00383-014-3467-0
更新日期:2014-03-01 00:00:00
abstract::Pediatric germ cell tumors comprise 1-3% of all malignant pediatric tumors and are found in variable locations. We present the case of a term 3.7 kg neonate who was found to have a giant liver mass at birth, later determined to be an immature teratoma arising from the hepatoduodenal ligament. This case report and imag...
journal_title:Pediatric surgery international
pub_type: 杂志文章
doi:10.1007/s00383-017-4123-2
更新日期:2017-09-01 00:00:00
abstract:BACKGROUND:Preoperative factors have been correlated with pre-incision hypotension (PIH) in children undergoing surgery, suggesting that PIH can be predicted through preoperative screening. We studied blood pressure (BP) changes in the 12 min following the induction of anesthesia to study the incidence of post-inductio...
journal_title:Pediatric surgery international
pub_type: 杂志文章
doi:10.1007/s00383-018-4359-5
更新日期:2018-12-01 00:00:00
abstract::The embryogenesis of a previously unreported mesothelial pouch containing a Meckel's diverticulum is presented. ...
journal_title:Pediatric surgery international
pub_type: 杂志文章
doi:10.1007/BF01076960
更新日期:1997-07-01 00:00:00
abstract::An infant developed massive lower gastrointestinal haemorrhage secondary to necrotising enterocolitis after cardiac surgery. The haemorrhage was only controlled following a colectomy. ...
journal_title:Pediatric surgery international
pub_type: 杂志文章
doi:10.1007/s003830050613
更新日期:1999-07-01 00:00:00
abstract::To assess the important factors for successful primary closure in staged reconstruction of bladder exstrophy, 25 patients (18 males, 7 females) underwent primary bladder closure during the years 1993-1997. Twenty-one were more than 72 h old; all of these underwent bilateral posterior iliac osteotomies followed by prim...
journal_title:Pediatric surgery international
pub_type: 杂志文章
doi:10.1007/s003830050721
更新日期:2000-01-01 00:00:00
abstract::Acute abdomen is caused by a wide variety of etiologies, many of which require surgical intervention. Two boys were hospitalized for acute abdominal pain and low-grade fever. Physical examination revealed epigastric and right upper abdominal fullness, and laboratory studies showed elevated erythrocyte sedimentation ra...
journal_title:Pediatric surgery international
pub_type: 杂志文章
doi:10.1007/s00383-002-0942-9
更新日期:2003-11-01 00:00:00
abstract:PURPOSE:The clinical application of autologous tissue-engineered skin analogs is an important strategy to cover large skin defects. Investigating biological dynamics, such as reinnervation after transplantation, is essential to improve the quality of such skin analogs. Previously, we have examined that our skin substit...
journal_title:Pediatric surgery international
pub_type: 杂志文章
doi:10.1007/s00383-016-3978-y
更新日期:2016-12-01 00:00:00
abstract::The Malone antegrade enema (MACE) and the caecostomy button (CB) are two methods of achieving colonic lavage in constipated children with faecal soiling. We reviewed our experience with the MACE and CB, aiming to compare results, complications, and outcomes. Between June 1998 and August 2002, 37 children (15 boys) und...
journal_title:Pediatric surgery international
pub_type: 杂志文章
doi:10.1007/s00383-004-1220-9
更新日期:2004-07-01 00:00:00
abstract::An isolated bowel segment (IBS) is a viable loop of bowel that is completely free of its mesenteric attachments. In this study, omentoenteropexy (OEP) was performed in 5-cm-long IBS of rats in order to provide neovascularization. Thirty-two rats were divided into four groups. After OEP, mesenteric division of the IBSs...
journal_title:Pediatric surgery international
pub_type: 杂志文章
doi:10.1007/BF01076940
更新日期:1997-07-01 00:00:00
abstract::High ligation of spermatic vessels (HLSV) and low ligation of spermatic vessels (LLSV) have been described in the surgical treatment of high undescended testis. Testicular capillary blood flow utilizing laser Doppler flowmetry after these maneuvers has not been studied thus far; hence, this study was designed. Thirty ...
journal_title:Pediatric surgery international
pub_type: 杂志文章
doi:10.1007/s00383-003-1018-1
更新日期:2003-07-01 00:00:00
abstract::The case of a 9-year-old girl with a Mycobacterium tuberculosis inflammatory myofibroblastic tumor (IMT) of the left lobe of the liver is reported. The tumor was surgically excised and had histological features diagnostic of IMT, a positive Ziehl-Nielsen staining for acid-fast bacilli and a positive polymerase chain r...
journal_title:Pediatric surgery international
pub_type: 杂志文章
doi:10.1007/s00383-009-2361-7
更新日期:2009-05-01 00:00:00
abstract::Rhizomelic chondrodysplasia punctata (RCP), a rare autosomal recessive disease characterized by a disorder of peroxisome metabolism, has been shown to affect multiple organ systems. A neonate presenting with a colonic perforation in the first few hours of life was subsequently diagnosed with RCP. A literature search r...
journal_title:Pediatric surgery international
pub_type: 杂志文章
doi:10.1007/s00383-005-1426-5
更新日期:2005-08-01 00:00:00
abstract::We reviewed our clinical experience of using a computer-powered right angle linear cutter (CPRALC) for Collis-Nissen fundoplication (CNF) in three children with gastroesophageal reflux (GER) or failed Nissen associated with short esophagus. Case 1 was a 13-month-old female with persistent GER after type-C esophageal a...
journal_title:Pediatric surgery international
pub_type: 杂志文章
doi:10.1007/s00383-009-2455-2
更新日期:2009-11-01 00:00:00
abstract:PURPOSE:Since 1999, complete primary repair of exstrophy has represented a valid alternative in the treatment of exstrophy patients, offering one- stage reconstruction for all components of this malformation in newborns. The vast majority of cases are currently approached within 48/72 h of life, and risk of vascular in...
journal_title:Pediatric surgery international
pub_type: 杂志文章
doi:10.1007/s00383-010-2745-8
更新日期:2011-04-01 00:00:00
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journal_title:Pediatric surgery international
pub_type: 杂志文章
doi:10.1007/s00383-014-3590-y
更新日期:2014-10-01 00:00:00
abstract::A small phallus causes great concern regarding genital adequacy. A concealed penis, although of normal size, appears small either because it is buried in prepubic tissues, enclosed in scrotal tissue penis palmatus (PP), or trapped due to phimosis or a scar following circumcision or trauma. From July 1978 to January 20...
journal_title:Pediatric surgery international
pub_type: 杂志文章
doi:10.1007/s00383-002-0770-y
更新日期:2002-12-01 00:00:00
abstract::In many very-low-birth-weight (VLBW) infants the ductus arteriosus fails to close spontaneously, and they subsequently develop signs and symptoms of poor tissue perfusion and heart failure. This study evalutes the results of early surgical closure of patent ductus arteriosus (PDA). We retrospectively reviewed the reco...
journal_title:Pediatric surgery international
pub_type: 杂志文章
doi:10.1007/s003830000515
更新日期:2001-07-01 00:00:00
abstract:BACKGROUND:Variation exists in the diagnostic testing for gastroesophageal reflux (GER) in infants and in the application of surgical therapy. There has been an increase in antireflux surgery (ARS) since the development of laparoscopy but the outcomes in high-risk infants is unclear. This study examines the results of ...
journal_title:Pediatric surgery international
pub_type: 杂志文章
doi:10.1007/s00383-018-4281-x
更新日期:2018-07-01 00:00:00
abstract::Retrocaval ureter is abnormal looping of the proximal ureter behind the inferior vena cava. The aberrant anatomy results in the compression of the ureter causing hydronephrosis. This is a very infrequent cause of hydronephrosis in children. Association of retrocaval ureter with a stone in the looping segment of the ur...
journal_title:Pediatric surgery international
pub_type: 杂志文章
doi:10.1007/s00383-010-2607-4
更新日期:2010-08-01 00:00:00
abstract:BACKGROUND:The natural history of patients with antenatally diagnosed congenital cystic adenomatoid malformation of the lung (CCAM) is still fully unknown. In symptomatic patients with respiratory distress, an operation is performed during the neonatal period. However, in asymptomatic patients, the optimal timing of th...
journal_title:Pediatric surgery international
pub_type: 杂志文章
doi:10.1007/s00383-009-2431-x
更新日期:2009-09-01 00:00:00
abstract::Parathyroid carcinoma is unusual and its intrathyroidal variant is extremely rare. Therefore, few cases have been reported to describe a case of parathyroid carcinoma located inside the thyroid gland. The case corresponds to a 14-year-old girl who came to the office with a severe osteoarticular disease, depression, ca...
journal_title:Pediatric surgery international
pub_type: 杂志文章
doi:10.1007/s00383-011-2904-6
更新日期:2011-12-01 00:00:00