Transcriptome Profile of Yeast Strain Used for Biological Wine Aging Revealed Dynamic Changes of Gene Expression in Course of Flor Development.

Abstract:

:Flor strains of Saccharomyces cerevisiae are principal microbial agents responsible for biological wine aging used for production of sherry-like wines. The flor yeast velum formed on the surface of fortified fermented must is a major adaptive and technological characteristic of flor yeasts that helps them to withstanding stressful winemaking conditions and ensures specific biochemical and sensory oxidative alterations typical for sherry wines. We have applied RNAseq technology for transcriptome analysis of an industrial flor yeast strain at different steps of velum development over 71 days under experimental winemaking conditions. Velum growth and maturation was accompanied by accumulation of aldehydes and acetales. We have identified 1490 differentially expressed genes including 816 genes upregulated and 674 downregulated more than 2-fold at mature biofilm stage as compared to the early biofilm. Distinct expression patterns of genes involved in carbon and nitrogen metabolism, respiration, cell cycle, DNA repair, cell adhesion, response to various stresses were observed. Many genes involved in response to different stresses, oxidative carbon metabolism, high affinity transport of sugars, glycerol utilization, sulfur metabolism, protein quality control and recycling, cell wall biogenesis, apoptosis were induced at the mature biofilm stage. Strong upregulation was observed for FLO11 flocculin while expression of other flocculins remained unaltered or moderately downregulated. Downregulated genes included those for proteins involved in glycolysis, transportation of ions, metals, aminoacids, sugars, indicating repression of some major transport and metabolic process at the mature biofilm stage. Presented results are important for in-depth understanding of cell response elicited by velum formation and sherry wine manufacturing conditions, and for the comprehension of relevant regulatory mechanisms. Such knowledge may help to better understand the molecular mechanisms that flor yeasts use to adapt to winemaking environments, establish the functions of previously uncharacterized genes, improve the technology of sherry- wine production, and find target genes for strain improvement.

journal_name

Front Microbiol

authors

Mardanov AV,Eldarov MA,Beletsky AV,Tanashchuk TN,Kishkovskaya SA,Ravin NV

doi

10.3389/fmicb.2020.00538

subject

Has Abstract

pub_date

2020-04-03 00:00:00

pages

538

issn

1664-302X

journal_volume

11

pub_type

杂志文章
  • De novo Transcriptome Analysis of Rhizoctonia solani AG1 IA Strain Early Invasion in Zoysia japonica Root.

    abstract::Zoysia japonica brown spot was caused by necrotrophic fungus Rhizoctonia solani invasion, which led to severe financial loss in city lawn and golf ground maintenance. However, little was known about the molecular mechanism of R. solani pathogenicity in Z. japonica. In this study we examined early stage interaction bet...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2016.00708

    authors: Zhu C,Ai L,Wang L,Yin P,Liu C,Li S,Zeng H

    更新日期:2016-05-18 00:00:00

  • Bacterial Seed Endophytes of Domesticated Cucurbits Antagonize Fungal and Oomycete Pathogens Including Powdery Mildew.

    abstract::The cucurbit vegetables, including cucumbers, melons and pumpkins, have been cultivated for thousands of years without fungicides. However, their seed germination stage is prone to be infected by soil-borne fungal and oomycete pathogens. Endophytes are symbionts that reside inside plant tissues including seeds. Seed e...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2018.00042

    authors: Khalaf EM,Raizada MN

    更新日期:2018-02-05 00:00:00

  • Offspring Microbiomes Differ Across Breeding Sites in a Panmictic Species.

    abstract::High dispersal rates are known to homogenize host's population genetic structure in panmictic species and to disrupt host local adaptation to the environment. Long-distance dispersal might also spread micro-organisms across large geographical areas. However, so far, to which extent selection mechanisms that shape host...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2019.00035

    authors: Gillingham MAF,Béchet A,Cézilly F,Wilhelm K,Rendón-Martos M,Borghesi F,Nissardi S,Baccetti N,Azafzaf H,Menke S,Kayser Y,Sommer S

    更新日期:2019-02-06 00:00:00

  • Temporal Bacterial Surveillance of Salmon Aquaculture Sites Indicates a Long Lasting Benthic Impact With Minimal Recovery.

    abstract::Coastal aquaculture has experienced substantial growth in the last few decades and associated impacts on natural environments are of increasing importance. Understanding both the effects of aquaculture on marine ecosystems and the processes of recovery during fallowing periods is crucial for the development of a more ...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2018.03054

    authors: Verhoeven JTP,Salvo F,Knight R,Hamoutene D,Dufour SC

    更新日期:2018-12-12 00:00:00

  • Binding of Campylobacter jejuni FliW Adjacent to the CsrA RNA-Binding Pockets Modulates CsrA Regulatory Activity.

    abstract::Campylobacter jejuni CsrA is an mRNA-binding, post-transcriptional regulator that controls many metabolic- and virulence-related characteristics of this important pathogen. In contrast to E. coli CsrA, whose activity is modulated by binding to small non-coding RNAs (sRNAs), C. jejuni CsrA activity is controlled by bin...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2020.531596

    authors: Bogacz M,El Abbar FM,Cox CA,Li J,Fiedler JS,Tran LKH,Tran PMH,Daugherty CL,Blake KH,Wang Z,Azadi P,Thompson SA

    更新日期:2021-01-11 00:00:00

  • Specific in vitro interferon-gamma and IL-2 production as biomarkers during treatment of chronic Q fever.

    abstract:BACKGROUND:Antibiotic treatment of chronic Q fever is cumbersome and of long duration. To monitor treatment, there is a need for alternative biomarkers. Coxiella burnetii-specific interferon (IFN)-γ and interleukin (IL)-2 production reflect the type of effector and memory T-cell response. In chronic Q fever, C. burneti...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2015.00093

    authors: Schoffelen T,Wegdam-Blans MC,Ammerdorffer A,Pronk MJ,Soethoudt YE,Netea MG,van der Meer JW,Bleeker-Rovers CP,van Deuren M

    更新日期:2015-02-12 00:00:00

  • Microbial community composition of transiently wetted Antarctic Dry Valley soils.

    abstract::During the summer months, wet (hyporheic) soils associated with ephemeral streams and lake edges in the Antarctic Dry Valleys (DVs) become hotspots of biological activity and are hypothesized to be an important source of carbon and nitrogen for arid DV soils. Recent research in the DV has focused on the geochemistry a...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2015.00009

    authors: Niederberger TD,Sohm JA,Gunderson TE,Parker AE,Tirindelli J,Capone DG,Carpenter EJ,Cary SC

    更新日期:2015-01-28 00:00:00

  • MALDI-TOF mass spectrometry: an emerging technology for microbial identification and diagnosis.

    abstract::Currently microorganisms are best identified using 16S rRNA and 18S rRNA gene sequencing. However, in recent years matrix assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) has emerged as a potential tool for microbial identification and diagnosis. During the MALDI-TOF MS process, mic...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章,评审

    doi:10.3389/fmicb.2015.00791

    authors: Singhal N,Kumar M,Kanaujia PK,Virdi JS

    更新日期:2015-08-05 00:00:00

  • Potential Risk Areas of Aedes albopictus in South-Eastern Iran: A Vector of Dengue Fever, Zika, and Chikungunya.

    abstract::The possibility of the rapid and global spread of Zika, chikungunya, yellow fever, and dengue fever by Aedes albopictus is well documented and may be facilitated by changes in climate. To avert and manage health risks, climatic and topographic information can be used to model and forecast which areas may be most prone...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2017.01660

    authors: Nejati J,Bueno-Marí R,Collantes F,Hanafi-Bojd AA,Vatandoost H,Charrahy Z,Tabatabaei SM,Yaghoobi-Ershadi MR,Hasanzehi A,Shirzadi MR,Moosa-Kazemi SH,Sedaghat MM

    更新日期:2017-09-05 00:00:00

  • Promotion of Para-Chlorophenol Reduction and Extracellular Electron Transfer in an Anaerobic System at the Presence of Iron-Oxides.

    abstract::Anaerobic dechlorination of chlorophenols often subjects to their toxicity and recalcitrance, presenting low loading rate and poor degradation efficiency. In this study, in order to accelerate p-chlorophenol (p-CP) reduction and extracellular electron transfer in an anaerobic system, three iron-oxide nanoparticles, na...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2018.02052

    authors: Jiang X,Chen Y,Hou C,Liu X,Ou C,Han W,Sun X,Li J,Wang L,Shen J

    更新日期:2018-08-30 00:00:00

  • Validating an Automated Nucleic Acid Extraction Device for Omics in Space Using Whole Cell Microbial Reference Standards.

    abstract::NASA has made great strides in the past five years to develop a suite of instruments for the International Space Station in order to perform molecular biology in space. However, a key piece of equipment that has been lacking is an instrument that can extract nucleic acids from an array of complex human and environment...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2020.01909

    authors: Urbaniak C,Wong S,Tighe S,Arumugam A,Liu B,Parker CW,Wood JM,Singh NK,Skorupa DJ,Peyton BM,Jenson R,Karouia F,Dragon J,Venkateswaran K

    更新日期:2020-08-21 00:00:00

  • Simultaneous Supplementation of Bacillus subtilis and Antibiotic Growth Promoters by Stages Improved Intestinal Function of Pullets by Altering Gut Microbiota.

    abstract::Early nutrition of pullets could determine the overall development and the performance of laying hens. With the aim to reduce the use of antibiotic growth promoters (AGPs) and to maintain the growth and development of pullets, the effect of simultaneous short-termed supplementation of AGPs (bacitracin zinc 20 mg/kg an...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2018.02328

    authors: Li X,Wu S,Li X,Yan T,Duan Y,Yang X,Duan Y,Sun Q,Yang X

    更新日期:2018-10-12 00:00:00

  • The Heat Shock Protein 70 Family of Chaperones Regulates All Phases of the Enterovirus A71 Life Cycle.

    abstract::Enterovirus A71 (EV-A71) is one of the major etiologic agents causing hand, foot, and mouth disease (HFMD) in children and occasionally causes severe neurological diseases or even death. EV-A71 replicates rapidly in host cells. For a successful infection, viruses produce large quantities of viral proteins in a short p...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2020.01656

    authors: Su YS,Hsieh PY,Li JS,Pao YH,Chen CJ,Hwang LH

    更新日期:2020-07-14 00:00:00

  • Oxalic Acid Production in Clarireedia jacksonii Is Dictated by pH, Host Tissue, and Xylan.

    abstract::Dollar spot is caused by the fungus Clarireedia jacksonii and is the most common disease of golf course turfgrass in temperate climates. Oxalic acid (OA) is an important pathogenicity factor in other fungal plant pathogens, such as the dicot pathogen Sclerotinia sclerotiorum, but its role in C. jacksonii pathogenicity...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2020.01732

    authors: Townsend RV,Rioux RA,Kabbage M,Stephens C,Kerns JP,Koch P

    更新日期:2020-08-04 00:00:00

  • 13C-DNA-SIP Distinguishes the Prokaryotic Community That Metabolizes Soybean Residues Produced Under Different CO2 Concentrations.

    abstract::The amendment of crop residues produced under elevated CO2 (eCO2) may alter soil microbial community structure and their functions on residue decomposition and carbon (C) cycling in soil. The key to understanding this process is to elucidate the structure of prokaryotic communities that metabolize crop residues derive...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2019.02184

    authors: Wang Y,Yu Z,Li Y,Wang G,Tang C,Liu X,Liu J,Xie Z,Jin J

    更新日期:2019-09-24 00:00:00

  • Pseudomonas aeruginosa Swarmer Cells Adaptation Toward UVc Radiations.

    abstract::Swarming is the most rapid surface motility allowing Pseudomonas aeruginosa bacteria to rapidly colonize new surfaces. However, swarming behavior is affected by environmental factors like ultraviolet irradiation (UVc). UVc radiation is the most disinfection technology usually applied for wastewater and proven to be ef...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2019.00556

    authors: Ghorbal SKB,Chourabi K,Maalej L,Ammar AB,Ouzari HI,Hassen A,Jaafoura H,Chatti A

    更新日期:2019-04-02 00:00:00

  • Diversity-Stability Dynamics of the Amphibian Skin Microbiome and Susceptibility to a Lethal Viral Pathogen.

    abstract::Variation among animals in their host-associated microbial communities is increasingly recognized as a key determinant of important life history traits including growth, metabolism, and resistance to disease. Quantitative estimates of the factors shaping the stability of host microbiomes over time at the individual le...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2019.02883

    authors: Harrison XA,Price SJ,Hopkins K,Leung WTM,Sergeant C,Garner TWJ

    更新日期:2019-12-20 00:00:00

  • Diversity and Function of Capsular Polysaccharide in Acinetobacter baumannii.

    abstract::The Gram-negative opportunistic bacterium Acinetobacter baumannii is a significant cause of hospital-borne infections worldwide. Alarmingly, the rapid development of antimicrobial resistance coupled with the remarkable ability of isolates to persist on surfaces for extended periods of time has led to infiltration of A...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2018.03301

    authors: Singh JK,Adams FG,Brown MH

    更新日期:2019-01-09 00:00:00

  • Heavy metal driven co-selection of antibiotic resistance in soil and water bodies impacted by agriculture and aquaculture.

    abstract::The use of antibiotic agents as growth promoters was banned in animal husbandry to prevent the selection and spread of antibiotic resistance. However, in addition to antibiotic agents, heavy metals used in animal farming and aquaculture might promote the spread of antibiotic resistance via co-selection. To investigate...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2012.00399

    authors: Seiler C,Berendonk TU

    更新日期:2012-12-14 00:00:00

  • Isolation and Genomic Characterization of an Acinetobacter johnsonii Bacteriophage AJO2 From Bulking Activated Sludge.

    abstract::A novel Podoviridae lytic phage AJO2, specifically infecting Acinetobacter johnsonii, was isolated from bulking activated sludge. The one-step growth experiment showed that the latent period and burst size of AJO2 were estimated to be 30 min and 78.1 phages per infected cell, respectively. The viability test indicated...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2019.00266

    authors: Fan N,Yang M,Jin R,Qi R

    更新日期:2019-02-20 00:00:00

  • Sequence-based Analysis of the Vitis vinifera L. cv Cabernet Sauvignon Grape Must Mycobiome in Three South African Vineyards Employing Distinct Agronomic Systems.

    abstract::Recent microbiomic research of agricultural habitats has highlighted tremendous microbial biodiversity associated with such ecosystems. Data generated in vineyards have furthermore highlighted significant regional differences in vineyard biodiversity, hinting at the possibility that such differences might be responsib...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2015.01358

    authors: Setati ME,Jacobson D,Bauer FF

    更新日期:2015-11-30 00:00:00

  • Global Distribution of Anaerobic Ammonia Oxidation (Anammox) Bacteria - Field Surveys in Wetland, Dryland, Groundwater Aquifer and Snow.

    abstract::The discovery of anaerobic ammonia oxidation (anammox) expanded our knowledge on the microbial nitrogen cycle. Previous studies report that anammox bacteria are distributed in a wide range of habitats and plays significant roles in the global nitrogen cycle. However, most studies focus only on individual ecosystems or...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2019.02583

    authors: Wang Y,Xu L,Wang S,Ye F,Zhu G

    更新日期:2019-11-12 00:00:00

  • Complementary DNA/RNA-Based Profiling: Characterization of Corrosive Microbial Communities and Their Functional Profiles in an Oil Production Facility.

    abstract::DNA and RNA-based sequencing of the 16S rRNA gene and transcripts were used to assess the phylogenetic diversity of microbial communities at assets experiencing corrosion in an oil production facility. The complementary methodological approach, coupled with extensive bioinformatics analysis, allowed to visualize diffe...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2019.02587

    authors: Salgar-Chaparro SJ,Machuca LL

    更新日期:2019-11-07 00:00:00

  • Ff-nano, short functionalized nanorods derived from Ff (f1, fd, or M13) filamentous bacteriophage.

    abstract::F-specific filamentous phage of Escherichia coli (Ff: f1, M13, or fd) are long thin filaments (860 nm × 6 nm). They have been a major workhorse in display technologies and bionanotechnology; however, some applications are limited by the high length-to-diameter ratio of Ff. Furthermore, use of functionalized Ff outside...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2015.00316

    authors: Sattar S,Bennett NJ,Wen WX,Guthrie JM,Blackwell LF,Conway JF,Rakonjac J

    更新日期:2015-04-20 00:00:00

  • Diatom-Bacteria Interactions Modulate the Composition and Productivity of Benthic Diatom Biofilms.

    abstract::Benthic diatoms are dominant primary producers in intertidal mudflats and constitute a major source of organic carbon to consumers and decomposers residing within these ecosystems. They typically form biofilms whose species richness, community composition and productivity can vary in response to environmental drivers ...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2019.01255

    authors: Koedooder C,Stock W,Willems A,Mangelinckx S,De Troch M,Vyverman W,Sabbe K

    更新日期:2019-06-05 00:00:00

  • MALDI-TOF MS Affords Discrimination of Deinococcus aquaticus Isolates Obtained From Diverse Biofilm Habitats.

    abstract::Matrix-assisted Laser Desorption Ionization-Time of Flight Mass Spectroscopy (MALDI-TOF MS) has been used routinely over the past decade in clinical microbiology laboratories to rapidly characterize diverse microorganisms of medical importance both at the genus and species levels. Currently, there is keen interest in ...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2018.02442

    authors: Tuohy JM,Mueller-Spitz SR,Albert CM,Scholz-Ng SE,Wall ME,Noutsios GT,Gutierrez AJ,Sandrin TR

    更新日期:2018-10-15 00:00:00

  • Suspended Materials in River Waters Differentially Enrich Class 1 Integron- and IncP-1 Plasmid-Carrying Bacteria in Sediments.

    abstract::Aquatic ecosystems are frequently considered as the final receiving environments of anthropogenic pollutants such as pharmaceutical residues or antibiotic resistant bacteria, and as a consequence tend to form reservoirs of antibiotic resistance genes. Considering the global threat posed by the antibiotic resistance, t...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2018.01443

    authors: De la Cruz Barrón M,Merlin C,Guilloteau H,Montargès-Pelletier E,Bellanger X

    更新日期:2018-07-02 00:00:00

  • The Putative Methyltransferase TlLAE1 Is Involved in the Regulation of Peptaibols Production in the Biocontrol Fungus Trichoderma longibrachiatum SMF2.

    abstract::The biocontrol fungus Trichoderma longibrachiatum SMF2 secretes a large quantity of peptaibols that have been shown to have a range of biological activities and therefore great application values. However, the mechanism of the regulatory expression of peptaibols is still unclear. The putative methyltransferase LaeA/LA...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2020.01267

    authors: Shi JC,Shi WL,Zhou YR,Chen XL,Zhang YZ,Zhang X,Zhang WX,Song XY

    更新日期:2020-06-12 00:00:00

  • Nutrient Acquisition, Rather Than Stress Response Over Diel Cycles, Drives Microbial Transcription in a Hyper-Arid Namib Desert Soil.

    abstract::Hot desert surface soils are characterized by extremely low water activities for large parts of any annual cycle. It is widely assumed that microbial processes in such soils are very limited. Here we present the first metatranscriptomic survey of microbial community function in a low water activity hyperarid desert so...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2019.01054

    authors: León-Sobrino C,Ramond JB,Maggs-Kölling G,Cowan DA

    更新日期:2019-05-14 00:00:00

  • Corrigendum: Molecular Characterization of Salmonella Serovars Anatum and Ealing Associated with Two Historical Outbreaks, Linked to Contaminated Powdered Infant Formula.

    abstract::[This corrects the article on p. 1664 in vol. 7, PMID: 27818652.]. ...

    journal_title:Frontiers in microbiology

    pub_type: 已发布勘误

    doi:10.3389/fmicb.2017.00015

    authors: Gunn L,Finn S,Hurley D,Bai L,Wall E,Iversen C,Threlfall JE,Fanning S

    更新日期:2017-01-20 00:00:00