Abstract:
:F-specific filamentous phage of Escherichia coli (Ff: f1, M13, or fd) are long thin filaments (860 nm × 6 nm). They have been a major workhorse in display technologies and bionanotechnology; however, some applications are limited by the high length-to-diameter ratio of Ff. Furthermore, use of functionalized Ff outside of laboratory containment is in part hampered by the fact that they are genetically modified viruses. We have now developed a system for production and purification of very short functionalized Ff-phage-derived nanorods, named Ff-nano, that are only 50 nm in length. In contrast to standard Ff-derived vectors that replicate in E. coli and contain antibiotic-resistance genes, Ff-nano are protein-DNA complexes that cannot replicate on their own and do not contain any coding sequences. These nanorods show an increased resistance to heating at 70(∘)C in 1% SDS in comparison to the full-length Ff phage of the same coat composition. We demonstrate that functionalized Ff-nano particles are suitable for application as detection particles in sensitive and quantitative "dipstick" lateral flow diagnostic assay for human plasma fibronectin.
journal_name
Front Microbioljournal_title
Frontiers in microbiologyauthors
Sattar S,Bennett NJ,Wen WX,Guthrie JM,Blackwell LF,Conway JF,Rakonjac Jdoi
10.3389/fmicb.2015.00316subject
Has Abstractpub_date
2015-04-20 00:00:00pages
316issn
1664-302Xjournal_volume
6pub_type
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pub_type: 杂志文章,评审
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journal_title:Frontiers in microbiology
pub_type: 杂志文章
doi:10.3389/fmicb.2017.00527
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journal_title:Frontiers in microbiology
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doi:10.3389/fmicb.2019.00527
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pub_type: 杂志文章
doi:10.3389/fmicb.2020.00156
更新日期:2020-03-03 00:00:00
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journal_title:Frontiers in microbiology
pub_type: 杂志文章,评审
doi:10.3389/fmicb.2017.02065
更新日期:2017-11-01 00:00:00
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journal_title:Frontiers in microbiology
pub_type: 杂志文章
doi:10.3389/fmicb.2015.00512
更新日期:2015-05-27 00:00:00
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pub_type: 杂志文章
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pub_type: 杂志文章,评审
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journal_title:Frontiers in microbiology
pub_type: 杂志文章
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更新日期:2012-02-21 00:00:00