GABA-Producing Natural Dairy Isolate From Artisanal Zlatar Cheese Attenuates Gut Inflammation and Strengthens Gut Epithelial Barrier in vitro.

Abstract:

:Probiotic bacteria are recognized for their health-promoting properties, including maintenance of gut epithelial integrity and host immune system homeostasis. Taking into account the beneficial health-promoting effects of GABA, the presence of the gadB gene, encoding glutamate decarboxylase that converts L-glutamate to GABA, was analyzed in Lactic Acid Bacteria (LAB) natural isolates from Zlatar cheese. The results revealed that 52% of tested Lactobacillus spp. and 8% of Lactococcus spp. isolates harbor the gadB gene. Qualitative and quantitative analysis of GABA production performed by thin-layer chromatography (TLC) and high-performance liquid chromatography (HPLC) revealed the highest GABA production by Lactobacillus brevis BGZLS10-17. Since high GABA-producing LAB natural isolates are the most valuable source of naturally produced GABA, the probiotic properties of BGZLS10-17 were characterized. This study demonstrated high adhesion of BGZLS10-17 strain to Caco-2 cells and the ability to decrease the adhesion of Escherichia coli ATCC25922 and Salmonella enterica C29039. Treatment of differentiated Caco-2 cells monolayer with BGZLS10-17 supernatant containing GABA alleviated inflammation (production of IL-8) caused by IL-1β and significantly stimulated the expression of tight junction proteins (zonulin, occludin, and claudin 4), as well as the expression of TGF-β cytokine leading to the conclusion that immunosuppression and strengthening the tight junctions can have significant role in the maintenance of intestinal epithelial barrier integrity. Taken together the results obtained in this study support the idea that using of GABA producing BGZLS10-17 probiotic strain could be a good strategy to modulate immunological response in various inflammatory diseases, and at the same time, it could be a good candidate for adjunct starter culture for production of GABA-enriched dairy foods and beverages offering new perspectives in designing the novel functional foods.

journal_name

Front Microbiol

authors

Sokovic Bajic S,Djokic J,Dinic M,Veljovic K,Golic N,Mihajlovic S,Tolinacki M

doi

10.3389/fmicb.2019.00527

subject

Has Abstract

pub_date

2019-03-18 00:00:00

pages

527

issn

1664-302X

journal_volume

10

pub_type

杂志文章
  • PNA Length Restriction of Antibacterial Activity of Peptide-PNA Conjugates in Escherichia coli Through Effects of the Inner Membrane.

    abstract::Peptide Nucleic Acid (PNA)-peptide conjugates targeting essential bacterial genes are showing promise as antisense antimicrobials in drug discovery. Optimization has focused on selection of target genes and exact localization around the ribosome binding site, but surprisingly a length optimum around 10-12 nucleobases ...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2019.01032

    authors: Goltermann L,Yavari N,Zhang M,Ghosal A,Nielsen PE

    更新日期:2019-05-24 00:00:00

  • PromA Plasmids Are Instrumental in the Dissemination of Linuron Catabolic Genes Between Different Genera.

    abstract::PromA plasmids are broad host range (BHR) plasmids, which are often cryptic and hence have an uncertain ecological role. We present three novel PromA γ plasmids which carry genes associated with degradation of the phenylurea herbicide linuron, two of which originated from unrelated Hydrogenophaga hosts isolated from d...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2020.00149

    authors: Werner J,Nour E,Bunk B,Spröer C,Smalla K,Springael D,Öztürk B

    更新日期:2020-02-18 00:00:00

  • Ff-nano, short functionalized nanorods derived from Ff (f1, fd, or M13) filamentous bacteriophage.

    abstract::F-specific filamentous phage of Escherichia coli (Ff: f1, M13, or fd) are long thin filaments (860 nm × 6 nm). They have been a major workhorse in display technologies and bionanotechnology; however, some applications are limited by the high length-to-diameter ratio of Ff. Furthermore, use of functionalized Ff outside...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2015.00316

    authors: Sattar S,Bennett NJ,Wen WX,Guthrie JM,Blackwell LF,Conway JF,Rakonjac J

    更新日期:2015-04-20 00:00:00

  • Growth Mode and Carbon Source Impact the Surfaceome Dynamics of Lactobacillus rhamnosus GG.

    abstract::Bacterial biofilms have clear implications in disease and in food applications involving probiotics. Here, we show that switching the carbohydrate source from glucose to fructose increased the biofilm formation and the total surface-antigenicity of a well-known probiotic, Lactobacillus rhamnosus GG. Surfaceomes (all c...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2019.01272

    authors: Savijoki K,Nyman TA,Kainulainen V,Miettinen I,Siljamäki P,Fallarero A,Sandholm J,Satokari R,Varmanen P

    更新日期:2019-06-05 00:00:00

  • Aspergillus niger Secretes Citrate to Increase Iron Bioavailability.

    abstract::Aspergillus niger has an innate ability to secrete various organic acids, including citrate. The conditions required for A. niger citrate overproduction are well described, but the physiological reasons underlying extracellular citrate accumulation are not yet fully understood. One of the less understood culture condi...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2017.01424

    authors: Odoni DI,van Gaal MP,Schonewille T,Tamayo-Ramos JA,Martins Dos Santos VAP,Suarez-Diez M,Schaap PJ

    更新日期:2017-08-02 00:00:00

  • Genomic Analysis of Microbulbifer sp. Strain A4B-17 and the Characterization of Its Metabolic Pathways for 4-Hydroxybenzoic Acid Synthesis.

    abstract::The marine bacterium Microbulbifer sp. A4B-17 produces secondary metabolites such as 4-hydroxybenzoic acid (4HBA) and esters of 4HBA (parabens). 4HBA is a useful material in the synthesis of the liquid crystal. Parabens are man-made compounds that have been extensively used since the 1920s in the cosmetic, pharmaceuti...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2018.03115

    authors: Tian J,Zhu L,Wang W,Zhang L,Li Z,Zhao Q,Xing K,Feng Z,Peng X

    更新日期:2018-12-18 00:00:00

  • Corrigendum: Cross-Talk between Staphylococcus aureus and Other Staphylococcal Species via the agr Quorum Sensing System.

    abstract::[This corrects the article on p. 1733 in vol. 7, PMID: 27877157.]. ...

    journal_title:Frontiers in microbiology

    pub_type: 已发布勘误

    doi:10.3389/fmicb.2017.01075

    authors: Canovas J,Baldry M,Bojer MS,Andersen PS,Grzeskowiak PK,Stegger M,Damborg P,Olsen CA,Ingmer H

    更新日期:2017-06-08 00:00:00

  • Evaluation of Whole-Genome Sequence Method to Diagnose Resistance of 13 Anti-tuberculosis Drugs and Characterize Resistance Genes in Clinical Multi-Drug Resistance Mycobacterium tuberculosis Isolates From China.

    abstract::Background: Whole-genome sequencing (WGS) is a viable and financially feasible tool for timely and comprehensive diagnosis of drug resistance in developed countries. With the increase in the incidence of multidrug-resistant tuberculosis (MDR-TB), second-line anti-TB drugs are gaining importance. However, genetic resis...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2019.01741

    authors: Chen X,He G,Wang S,Lin S,Chen J,Zhang W

    更新日期:2019-07-31 00:00:00

  • The Role of Volatile Organic Compounds and Rhizosphere Competence in Mode of Action of the Non-pathogenic Fusarium oxysporum FO12 Toward Verticillium Wilt.

    abstract::Verticillium wilts caused by Verticillium spp. are among the most challenging plant diseases to control and affect numerous hosts worldwide. Due to the lack of effective, conventional control methods, integrated control strategies provide a promising approach to manage these diseases. The non-pathogenic Fusarium oxysp...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2019.01808

    authors: Mulero-Aparicio A,Cernava T,Turrà D,Schaefer A,Di Pietro A,López-Escudero FJ,Trapero A,Berg G

    更新日期:2019-08-02 00:00:00

  • Potential drivers of microbial community structure and function in Arctic spring snow.

    abstract::The Arctic seasonal snowpack can extend at times over a third of the Earth's land surface. This chemically dynamic environment interacts constantly with different environmental compartments such as atmosphere, soil and meltwater, and thus, strongly influences the entire biosphere. However, the microbial community asso...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2014.00413

    authors: Maccario L,Vogel TM,Larose C

    更新日期:2014-08-07 00:00:00

  • Long-Term Persistent GBV-B Infection and Development of a Chronic and Progressive Hepatitis C-Like Disease in Marmosets.

    abstract::It has been shown that infection of GB virus B (GBV-B), which is closely related to hepatitis C virus, develops acute self-resolving hepatitis in tamarins. In this study we sought to examine longitudinally the dynamics of viral and immunological status following GBV-B infection of marmosets and tamarins. Surprisingly,...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2011.00240

    authors: Iwasaki Y,Mori K,Ishii K,Maki N,Iijima S,Yoshida T,Okabayashi S,Katakai Y,Lee YJ,Saito A,Fukai H,Kimura N,Ageyama N,Yoshizaki S,Suzuki T,Yasutomi Y,Miyamura T,Kannagi M,Akari H

    更新日期:2011-12-07 00:00:00

  • Bacterial Community Composition and Potential Driving Factors in Different Reef Habitats of the Spermonde Archipelago, Indonesia.

    abstract::Coastal eutrophication is a key driver of shifts in bacterial communities on coral reefs. With fringing and patch reefs at varying distances from the coast the Spermonde Archipelago in southern Sulawesi, Indonesia offers ideal conditions to study the effects of coastal eutrophication along a spatially defined gradient...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2017.00662

    authors: Kegler HF,Lukman M,Teichberg M,Plass-Johnson J,Hassenrück C,Wild C,Gärdes A

    更新日期:2017-04-20 00:00:00

  • Influenza Virus Infection Induces Host Pyruvate Kinase M Which Interacts with Viral RNA-Dependent RNA Polymerase.

    abstract::Influenza virus RNA-dependent RNA polymerase (RdRp) is a heterotrimer of three viral proteins, PB1, PB2, and PA and is involved in both transcription and replication of the negative strand of the viral RNA (vRNA) genome. RdRp is multifunctional, possessing RNA polymerase, cap binding, and endonuclease activities. The ...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2017.00162

    authors: Miyake Y,Ishii K,Honda A

    更新日期:2017-02-09 00:00:00

  • Emerging Trends in Biological Treatment of Wastewater From Unconventional Oil and Gas Extraction.

    abstract::Unconventional oil and gas exploration generates an enormous quantity of wastewater, commonly referred to as flowback and produced water (FPW). Limited freshwater resources and stringent disposal regulations have provided impetus for FPW reuse. Organic and inorganic compounds released from the shale/brine formation, m...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章,评审

    doi:10.3389/fmicb.2020.569019

    authors: Acharya SM,Chakraborty R,Tringe SG

    更新日期:2020-09-09 00:00:00

  • Assessment of Ruminal Bacterial and Archaeal Community Structure in Yak (Bos grunniens).

    abstract::The aim of this study was to determine the microbial community composition in the rumen of yaks under different feeding regimes. Microbial communities were assessed by sequencing bacterial and archaeal 16S ribosomal RNA gene fragments obtained from yaks (Bos grunniens) from Qinghai-Tibetan Plateau, China. Samples were...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2017.00179

    authors: Zhou Z,Fang L,Meng Q,Li S,Chai S,Liu S,Schonewille JT

    更新日期:2017-02-07 00:00:00

  • H9N2 Avian Influenza Virus Protein PB1 Enhances the Immune Responses of Bone Marrow-Derived Dendritic Cells by Down-Regulating miR375.

    abstract::Polymerase basic protein 1 (PB1), the catalytic core of the influenza A virus RNA polymerase complex, is essential for viral transcription and replication. Dendritic cells (DCs) possess important antigen presenting ability and a crucial role in recognizing and clearing virus. MicroRNA (miRNA) influence the development...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2017.00287

    authors: Lin J,Xia J,Tu CZ,Zhang KY,Zeng Y,Yang Q

    更新日期:2017-03-22 00:00:00

  • Evolutionary Evidence of Algal Polysaccharide Degradation Acquisition by Pseudoalteromonas carrageenovora 9T to Adapt to Macroalgal Niches.

    abstract::About half of seaweed biomass is composed of polysaccharides. Most of these complex polymers have a marked polyanionic character. For instance, the red algal cell wall is mainly composed of sulfated galactans, agars and carrageenans, while brown algae contain alginate and fucose-containing sulfated polysaccharides (FC...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2018.02740

    authors: Gobet A,Barbeyron T,Matard-Mann M,Magdelenat G,Vallenet D,Duchaud E,Michel G

    更新日期:2018-11-22 00:00:00

  • Anti-tumoral Effects of Recombinant Lactococcus lactis Strain Secreting IL-17A Cytokine.

    abstract::Interleukin-17A (IL-17A) is a pro-inflammatory cytokine produced by TH17 cells that participates and contributes in host defense and autoimmune disease. We have recently reported antitumor properties of the probiotic strain of Lactobacillus casei BL23 in mice and TH17 cells was shown to play an important role in this ...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2018.03355

    authors: Jacouton E,Torres Maravilla E,Boucard AS,Pouderous N,Pessoa Vilela AP,Naas I,Chain F,Azevedo V,Langella P,Bermúdez-Humarán LG

    更新日期:2019-01-23 00:00:00

  • Study on the Viable but Non-culturable (VBNC) State Formation of Staphylococcus aureus and Its Control in Food System.

    abstract::A Viable but non-culturable (VBNC) state is a bacterial survival strategy under reverse conditions. It poses a significant challenge for public health and food safety. In this study, the effect of external environmental conditions including acid, nutrition, and salt concentrations on the formation of S. aureus VBNC st...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2020.599739

    authors: Li Y,Huang TY,Mao Y,Chen Y,Shi F,Peng R,Chen J,Yuan L,Bai C,Chen L,Wang K,Liu J

    更新日期:2020-11-26 00:00:00

  • Antimicrobial Resistance in Wildlife in Guadeloupe (French West Indies): Distribution of a Single bla CTX-M-1/IncI1/ST3 Plasmid Among Humans and Wild Animals.

    abstract::Limited data are available on the contribution of wildlife to the spread of antibacterial resistance. We determined the prevalence of resistance to antibiotics in Escherichia coli isolates collected from wild animals in 2013 and 2014 and the genetic basis for resistance to third-generation cephalosporin in Guadeloupe....

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2020.01524

    authors: Guyomard-Rabenirina S,Reynaud Y,Pot M,Albina E,Couvin D,Ducat C,Gruel G,Ferdinand S,Legreneur P,Le Hello S,Malpote E,Sadikalay S,Talarmin A,Breurec S

    更新日期:2020-07-10 00:00:00

  • First Report of Vibrio tubiashii Associated with a Massive Larval Mortality Event in a Commercial Hatchery of Scallop Argopecten purpuratus in Chile.

    abstract::The VPAP30 strain was isolated as the highly predominant bacteria from an episode of massive larval mortality occurring in a commercial culture of the Chilean scallop Argopecten purpuratus. The main aims of this study were, to characterize and identify the pathogenic strain using biochemical and molecular methods to d...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章,收录出版

    doi:10.3389/fmicb.2016.01473

    authors: Rojas R,Miranda CD,Santander J,Romero J

    更新日期:2016-09-20 00:00:00

  • Deconjugated Bile Salts Produced by Extracellular Bile-Salt Hydrolase-Like Activities from the Probiotic Lactobacillus johnsonii La1 Inhibit Giardia duodenalis In vitro Growth.

    abstract::Giardiasis, currently considered a neglected disease, is caused by the intestinal protozoan parasite Giardia duodenalis and is widely spread in human as well as domestic and wild animals. The lack of appropriate medications and the spread of resistant parasite strains urgently call for the development of novel therape...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2016.01453

    authors: Travers MA,Sow C,Zirah S,Deregnaucourt C,Chaouch S,Queiroz RM,Charneau S,Allain T,Florent I,Grellier P

    更新日期:2016-09-27 00:00:00

  • Whole Genome Sequence Analysis of CTX-M-15 Producing Klebsiella Isolates Allowed Dissecting a Polyclonal Outbreak Scenario.

    abstract::Extended-spectrum β-lactamase (ESBL) producing Klebsiella pneumoniae pose an important threat of infection with increased morbidity and mortality, especially for immunocompromised patients. Here, we use the rise of multidrug-resistant K. pneumoniae in a German neurorehabilitation center from April 2015 to April 2016 t...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2018.00322

    authors: Becker L,Fuchs S,Pfeifer Y,Semmler T,Eckmanns T,Korr G,Sissolak D,Friedrichs M,Zill E,Tung ML,Dohle C,Kaase M,Gatermann S,Rüssmann H,Steglich M,Haller S,Werner G

    更新日期:2018-02-23 00:00:00

  • Rapid Purification and Characterization of Mutant Origin Recognition Complexes in Saccharomyces cerevisiae.

    abstract::Purification of the origin recognition complex (ORC) from wild-type budding yeast cells more than two decades ago opened up doors to analyze the initiation of eukaryotic chromosomal DNA replication biochemically. Although revised methods to purify ORC from overproducing cells were reported later, purification of mutan...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2016.00521

    authors: Kawakami H,Ohashi E,Tsurimoto T,Katayama T

    更新日期:2016-04-18 00:00:00

  • Occurrence of Hepatitis E Virus in Pigs and Pork Cuts and Organs at the Time of Slaughter, Spain, 2017.

    abstract::Zoonotic hepatitis E, mainly caused by hepatitis E virus (HEV) genotype (gt) 3, is a foodborne disease that has emerged in Europe in recent decades. The main animal reservoir for genotype 3 is domestic pigs. Pig liver and liver derivates are considered the major risk products, and studies focused on the presence of HE...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2019.02990

    authors: García N,Hernández M,Gutierrez-Boada M,Valero A,Navarro A,Muñoz-Chimeno M,Fernández-Manzano A,Escobar FM,Martínez I,Bárcena C,González S,Avellón A,Eiros JM,Fongaro G,Domínguez L,Goyache J,Rodríguez-Lázaro D

    更新日期:2020-01-28 00:00:00

  • The Shift of the Intestinal Microbiome in the Innate Immunity-Deficient Mutant rde-1 Strain of C. elegans upon Orsay Virus Infection.

    abstract::The status of intestinal microbiota is a determinant of host health. However, the alteration of the gut microbiota caused by the innate immune response to virus infection is unclear. Caenorhabditis elegans and its natural virus Orsay provide an excellent model of host-virus interactions. We evaluated the intestinal mi...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2017.00933

    authors: Guo Y,Xun Z,Coffman SR,Chen F

    更新日期:2017-05-29 00:00:00

  • iTRAQ Proteomic Analysis Reveals That Metabolic Pathways Involving Energy Metabolism Are Affected by Tea Tree Oil in Botrytis cinerea.

    abstract::Tea tree oil (TTO) is a volatile essential oil obtained from the leaves of the Australian tree Melaleuca alternifolia by vapor distillation. Previously, we demonstrated that TTO has a strong inhibitory effect on Botrytis cinerea. This study investigates the underlying antifungal mechanisms at the molecular level. A pr...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2017.01989

    authors: Xu J,Shao X,Wei Y,Xu F,Wang H

    更新日期:2017-10-12 00:00:00

  • A Plasmid-Transposon Hybrid Mutagenesis System Effective in a Broad Range of Enterobacteria.

    abstract::Random transposon mutagenesis is a powerful technique used to generate libraries of genetic insertions in many different bacterial strains. Here we develop a system facilitating random transposon mutagenesis in a range of different Gram-negative bacterial strains, including Pectobacterium atrosepticum, Citrobacter rod...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2015.01442

    authors: Monson R,Smith DS,Matilla MA,Roberts K,Richardson E,Drew A,Williamson N,Ramsay J,Welch M,Salmond GP

    更新日期:2015-12-22 00:00:00

  • Programming Bacteria With Light-Sensors and Applications in Synthetic Biology.

    abstract::Photo-receptors are widely present in both prokaryotic and eukaryotic cells, which serves as the foundation of tuning cell behaviors with light. While practices in eukaryotic cells have been relatively established, trials in bacterial cells have only been emerging in the past few years. A number of light sensors have ...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章,评审

    doi:10.3389/fmicb.2018.02692

    authors: Liu Z,Zhang J,Jin J,Geng Z,Qi Q,Liang Q

    更新日期:2018-11-08 00:00:00

  • The molecular dimension of microbial species: 1. Ecological distinctions among, and homogeneity within, putative ecotypes of Synechococcus inhabiting the cyanobacterial mat of Mushroom Spring, Yellowstone National Park.

    abstract::Based on the Stable Ecotype Model, evolution leads to the divergence of ecologically distinct populations (e.g., with different niches and/or behaviors) of ecologically interchangeable membership. In this study, pyrosequencing was used to provide deep sequence coverage of Synechococcus psaA genes and transcripts over ...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2015.00590

    authors: Becraft ED,Wood JM,Rusch DB,Kühl M,Jensen SI,Bryant DA,Roberts DW,Cohan FM,Ward DM

    更新日期:2015-06-22 00:00:00