Lysine and novel hydroxylysine lipids in soil bacteria: amino acid membrane lipid response to temperature and pH in Pseudopedobacter saltans.

Abstract:

:Microbial decomposition of organic matter is an essential process in the global carbon cycle. The soil bacteria Pseudopedobacter saltans and Flavobacterium johnsoniae are both able to degrade complex organic molecules, but it is not fully known how their membrane structures are adapted to their environmental niche. The membrane lipids of these species were extracted and analyzed using high performance liquid chromatography-electrospray ionization/ion trap/mass spectrometry (HPLC-ESI/IT/MS) and high resolution accurate mass/mass spectrometry (HRAM/MS). Abundant unknown intact polar lipids (IPLs) from P. saltans were isolated and further characterized using amino acid analysis and two dimensional nuclear magnetic resonance (NMR) spectroscopy. Ornithine IPLs (OLs) with variable (hydroxy) fatty acid composition were observed in both bacterial species. Lysine-containing IPLs (LLs) were also detected in both species and were characterized here for the first time using HPLC-MS. Novel LLs containing hydroxy fatty acids and novel hydroxylysine lipids with variable (hydroxy) fatty acid composition were identified in P. saltans. The confirmation of OL and LL formation in F. johnsoniae and P. saltans and the presence of OlsF putative homologs in P. saltans suggest the OlsF gene coding protein is possibly involved in OL and LL biosynthesis in both species, however, potential pathways of OL and LL hydroxylation in P. saltans are still undetermined. Triplicate cultures of P. saltans were grown at three temperature/pH combinations: 30°C/pH 7, 15°C/pH 7, and 15°C/pH 9. The fractional abundance of total amino acid containing IPLs containing hydroxylated fatty acids was significantly higher at higher temperature, and the fractional abundance of lysine-containing IPLs was significantly higher at lower temperature and higher pH. These results suggest that these amino acid-containing IPLs, including the novel hydroxylysine lipids, could be involved in temperature and pH stress response of soil bacteria.

journal_name

Front Microbiol

authors

Moore EK,Hopmans EC,Rijpstra WI,Sánchez-Andrea I,Villanueva L,Wienk H,Schoutsen F,Stams AJ,Sinninghe Damsté JS

doi

10.3389/fmicb.2015.00637

subject

Has Abstract

pub_date

2015-06-29 00:00:00

pages

637

issn

1664-302X

journal_volume

6

pub_type

杂志文章
  • Chemoorganotrophic Bacteria From Lake Fryxell, Antarctica, Including Pseudomonas Strain LFY10, a Cold-Adapted, Halotolerant Bacterium Useful in Teaching Labs.

    abstract::Lake Fryxell, situated in the McMurdo Dry Valleys of Antarctica, is an intriguing aquatic ecosystem because of its perennial ice cover, highly stratified water column, and extreme physicochemical conditions, which collectively restrict lake biodiversity to solely microbial forms. To expand our current understanding of...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2019.00156

    authors: Baker JM,Vander Schaaf NA,Cunningham AMG,Hang AC,Reeves CL,Huffman ER,Riester CJ,Madigan MT,Sattley WM

    更新日期:2019-02-06 00:00:00

  • Genetic and Toxigenic Variability within Aspergillus flavus Population Isolated from Maize in Two Diverse Environments in Kenya.

    abstract::Aspergillus flavus is the main producer of carcinogenic aflatoxins in agricultural commodities such as maize. This fungus occurs naturally on crops, and produces aflatoxins when environmental conditions are favorable. The aim of this study is to analyse the genetic variability among 109 A. flavus isolates previously r...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2018.00057

    authors: Okoth S,De Boevre M,Vidal A,Diana Di Mavungu J,Landschoot S,Kyallo M,Njuguna J,Harvey J,De Saeger S

    更新日期:2018-01-26 00:00:00

  • Grapevine leafroll-associated virus 3.

    abstract::Grapevine leafroll disease (GLD) is one of the most important grapevine viral diseases affecting grapevines worldwide. The impact on vine health, crop yield, and quality is difficult to assess due to a high number of variables, but significant economic losses are consistently reported over the lifespan of a vineyard i...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2013.00082

    authors: Maree HJ,Almeida RP,Bester R,Chooi KM,Cohen D,Dolja VV,Fuchs MF,Golino DA,Jooste AE,Martelli GP,Naidu RA,Rowhani A,Saldarelli P,Burger JT

    更新日期:2013-04-16 00:00:00

  • Dynamics of Inorganic Nutrients in Intertidal Sediments: Porewater, Exchangeable, and Intracellular Pools.

    abstract::The study of inorganic nutrients dynamics in shallow sediments usually focuses on two main pools: porewater (PW) nutrients and exchangeable (EX) ammonium and phosphate. Recently, it has been found that microphytobenthos (MPB) and other microorganisms can accumulate large amounts of nutrients intracellularly (IC), high...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2016.00761

    authors: Garcia-Robledo E,Bohorquez J,Corzo A,Jimenez-Arias JL,Papaspyrou S

    更新日期:2016-05-26 00:00:00

  • Genetic Characterization of a bla VIM-24-Carrying IncP-7β Plasmid p1160-VIM and a bla VIM-4-Harboring Integrative and Conjugative Element Tn6413 From Clinical Pseudomonas aeruginosa.

    abstract::This study presents three novel integrons In1394, In1395, and In1443, three novel unit transposons Tn6392, Tn6393, and Tn6403, one novel conjugative element (ICE) Tn6413, and the first sequenced IncP-7 resistance plasmid p1160-VIM from clinical Pseudomonas aeruginosa. Detailed sequence comparison of p1160-VIM (carryin...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2019.00213

    authors: Zeng L,Zhan Z,Hu L,Jiang X,Zhang Y,Feng J,Gao B,Zhao Y,Yang W,Yang H,Yin Z,Zhou D

    更新日期:2019-02-26 00:00:00

  • Probiotic Supplements: Hope or Hype?

    abstract::Probiotic bacteria have been associated with various health benefits and included in overwhelming number of foods. Today, probiotic supplements are consumed with increasing regularity and record a rapidly growing economic value. With billions of heterogeneous populations of probiotics per serving, probiotic supplement...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2020.00160

    authors: Wang Y,Jiang Y,Deng Y,Yi C,Wang Y,Ding M,Liu J,Jin X,Shen L,He Y,Wu X,Chen X,Sun C,Zheng M,Zhang R,Ye H,An H,Wong A

    更新日期:2020-02-28 00:00:00

  • Designing and Engineering Methylorubrum extorquens AM1 for Itaconic Acid Production.

    abstract::Methylorubrum extorquens (formerly Methylobacterium extorquens) AM1 is a methylotrophic bacterium with a versatile lifestyle. Various carbon sources including acetate, succinate and methanol are utilized by M. extorquens AM1 with the latter being a promising inexpensive substrate for use in the biotechnology industry....

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2019.01027

    authors: Lim CK,Villada JC,Chalifour A,Duran MF,Lu H,Lee PKH

    更新日期:2019-05-09 00:00:00

  • Microbial Communities in a High Arctic Polar Desert Landscape.

    abstract::The High Arctic is dominated by polar desert habitats whose microbial communities are poorly understood. In this study, we used next generation sequencing to describe the α- and β-diversity of microbial communities in polar desert soils from the Kongsfjorden region of Svalbard. Ten phyla dominated the soils and accoun...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2016.00419

    authors: McCann CM,Wade MJ,Gray ND,Roberts JA,Hubert CR,Graham DW

    更新日期:2016-03-31 00:00:00

  • Constructing and Characterizing Bacteriophage Libraries for Phage Therapy of Human Infections.

    abstract::Phage therapy requires libraries of well-characterized phages. Here we describe the generation of phage libraries for three target species: Escherichia coli, Pseudomonas aeruginosa, and Enterobacter cloacae. The basic phage characteristics on the isolation host, sequence analysis, growth properties, and host range and...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2019.02537

    authors: Gibson SB,Green SI,Liu CG,Salazar KC,Clark JR,Terwilliger AL,Kaplan HB,Maresso AW,Trautner BW,Ramig RF

    更新日期:2019-11-12 00:00:00

  • Modeling the Inactivation of Intestinal Pathogenic Escherichia coli O157:H7 and Uropathogenic E. coli in Ground Chicken by High Pressure Processing and Thymol.

    abstract::Disease causing Escherichia coli commonly found in meat and poultry include intestinal pathogenic E. coli (iPEC) as well as extraintestinal types such as the Uropathogenic E. coli (UPEC). In this study we compared the resistance of iPEC (O157:H7) to UPEC in chicken meat using High Pressure Processing (HPP) in with (th...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2016.00920

    authors: Chien SY,Sheen S,Sommers CH,Sheen LY

    更新日期:2016-06-14 00:00:00

  • Biotechnology and pasta-making: lactic Acid bacteria as a new driver of innovation.

    abstract::Cereals-derived foods represent a key constituent in the diet of many populations. In particular, pasta is consumed in large quantities throughout the world in reason of its nutritive importance, containing significant amounts of complex carbohydrates, proteins, B-vitamins, and iron. Lactic acid bacteria (LAB) are a h...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2012.00094

    authors: Capozzi V,Russo P,Fragasso M,De Vita P,Fiocco D,Spano G

    更新日期:2012-03-15 00:00:00

  • Simultaneous Activation of Iron- and Thiol-Based Sensor-Regulator Systems by Redox-Active Compounds.

    abstract::Bacteria in natural habitats are exposed to myriad redox-active compounds (RACs), which include producers of reactive oxygen species (ROS) and reactive electrophile species (RES) that alkylate or oxidize thiols. RACs can induce oxidative stress in cells and activate response pathways by modulating the activity of sens...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2017.00139

    authors: Lee KL,Yoo JS,Oh GS,Singh AK,Roe JH

    更新日期:2017-02-02 00:00:00

  • Natural Killer Cell Functions during the Innate Immune Response to Pathogenic Streptococci.

    abstract::Dendritic cells (DCs) and NK cells play a crucial role in the first phase of host defense against infections. Group B Streptococcus (GBS) and Streptococcus suis are encapsulated streptococci causing severe systemic inflammation, leading to septicemia and meningitis. Yet, the involvement of NK cells in the innate immun...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2017.01196

    authors: Lemire P,Galbas T,Thibodeau J,Segura M

    更新日期:2017-06-29 00:00:00

  • Surface (S) Layer Proteins of Lactobacillus acidophilus Block Virus Infection via DC-SIGN Interaction.

    abstract::Alphaviruses and flaviviruses are important human pathogens that include Chikungunya virus (CHIKV), Dengue virus (DENV), and Zika virus (ZIKV), which can cause diseases in humans ranging from arthralgia to hemorrhagic fevers and microcephaly. It was previously shown that treatment with surface layer (S-layer) protein,...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2019.00810

    authors: Prado Acosta M,Geoghegan EM,Lepenies B,Ruzal S,Kielian M,Martinez MG

    更新日期:2019-04-16 00:00:00

  • Allergies, Helicobacter pylori and the continental enigmas.

    abstract::Helicobacter pylori, a gastric pathogen, is known to be associated with gastric and duodenal ulcers, and is also a strong risk factor for the development of gastric cancer and lymphoma of the mucosal-associated lymphoid tissue. Ordinarily, this should make a strong case for its eradication at par with any other infect...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章,评审

    doi:10.3389/fmicb.2015.00578

    authors: Sitaraman R

    更新日期:2015-06-09 00:00:00

  • The Atypical Guanylate Kinase MoGuk2 Plays Important Roles in Asexual/Sexual Development, Conidial Septation, and Pathogenicity in the Rice Blast Fungus.

    abstract::Guanylate kinases (GKs), which convert guanosine monophosphate into guanosine diphosphate (GDP), are important for growth and mannose outer chain elongation of cell wall N-linked glycoproteins in yeast. Here, we identified the ortholog of Saccharomyces cerevisiae GK Guk1, named MoGuk1 and a novel family of fungal GKs ...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2017.02467

    authors: Cai X,Zhang X,Li X,Liu M,Liu X,Wang X,Zhang H,Zheng X,Zhang Z

    更新日期:2017-12-11 00:00:00

  • Dissimilatory nitrogen reduction in intertidal sediments of a temperate estuary: small scale heterogeneity and novel nitrate-to-ammonium reducers.

    abstract::The estuarine nitrogen cycle can be substantially altered due to anthropogenic activities resulting in increased amounts of inorganic nitrogen (mainly nitrate). In the past, denitrification was considered to be the main ecosystem process removing reactive nitrogen from the estuarine ecosystem. However, recent reports ...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2015.01124

    authors: Decleyre H,Heylen K,Van Colen C,Willems A

    更新日期:2015-10-14 00:00:00

  • Endosymbionts Alter Larva-to-Nymph Transstadial Transmission of Babesia microti in Rhipicephalus haemaphysaloides Ticks.

    abstract::Maternally inherited endosymbionts inhabit a variety of arthropods. Some of them can protect the arthropod host against a wide range of pathogens. However, very little is known about the association between endosymbionts and pathogen susceptibility in ticks. The present study investigated the effect of endosymbionts o...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2018.01415

    authors: Li LH,Zhang Y,Zhu D,Zhou XN

    更新日期:2018-06-27 00:00:00

  • Enzymatic modifications of exopolysaccharides enhance bacterial persistence.

    abstract::Biofilms are surface-attached communities of bacterial cells embedded in a self-produced matrix that are found ubiquitously in nature. The biofilm matrix is composed of various extracellular polymeric substances, which confer advantages to the encapsulated bacteria by protecting them from eradication. The matrix compo...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章,评审

    doi:10.3389/fmicb.2015.00471

    authors: Whitfield GB,Marmont LS,Howell PL

    更新日期:2015-05-15 00:00:00

  • AmrZ Regulates Swarming Motility Through Cyclic di-GMP-Dependent Motility Inhibition and Controlling Pel Polysaccharide Production in Pseudomonas aeruginosa PA14.

    abstract::Swarming is a surface-associated motile behavior that plays an important role in the rapid spread, colonization, and subsequent establishment of bacterial communities. In Pseudomonas aeruginosa, swarming is dependent upon a functional flagella and aided by the production of biosurfactants. AmrZ, a conserved transcript...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2019.01847

    authors: Hou L,Debru A,Chen Q,Bao Q,Li K

    更新日期:2019-08-14 00:00:00

  • HIV-1 Tat and cocaine mediated synaptopathy in cortical and midbrain neurons is prevented by the isoflavone Equol.

    abstract::Illicit drugs, such as cocaine, are known to increase the likelihood and severity of HIV-1 associated neurocognitive disorders (HAND). In the current studies synaptic integrity was assessed following exposure to low concentrations of the HIV-1 viral protein Tat 1-86B, with or without cocaine, by quantifying filamentou...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2015.00894

    authors: Bertrand SJ,Hu C,Aksenova MV,Mactutus CF,Booze RM

    更新日期:2015-09-08 00:00:00

  • Factors influencing the diversity of iron uptake systems in aquatic microorganisms.

    abstract::Iron (Fe) is an essential micronutrient for many processes in all living cells. Dissolved Fe (dFe) concentrations in the ocean are of the order of a few nM, and Fe is often a factor limiting primary production. Bioavailability of Fe in aquatic environments is believed to be primarily controlled through chelation by Fe...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2012.00362

    authors: Desai DK,Desai FD,Laroche J

    更新日期:2012-10-18 00:00:00

  • Heparin-Binding Hemagglutinin Adhesin (HBHA) Is Involved in Intracytosolic Lipid Inclusions Formation in Mycobacteria.

    abstract::The heparin-binding hemagglutinin adhesin (HBHA) is an important virulence factor of Mycobacterium tuberculosis. It is a surface-displayed protein that serves as an adhesin for non-phagocytic cells and is involved in extra-pulmonary dissemination of the tubercle bacillus. It is also an important latency antigen useful...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2018.02258

    authors: Raze D,Verwaerde C,Deloison G,Werkmeister E,Coupin B,Loyens M,Brodin P,Rouanet C,Locht C

    更新日期:2018-09-24 00:00:00

  • Drug Repurposing for Paracoccidioidomycosis Through a Computational Chemogenomics Framework.

    abstract::Paracoccidioidomycosis (PCM) is the most prevalent endemic mycosis in Latin America. The disease is caused by fungi of the genus Paracoccidioides and mainly affects low-income rural workers after inhalation of fungal conidia suspended in the air. The current arsenal of chemotherapeutic agents requires long-term admini...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2019.01301

    authors: de Oliveira AA,Neves BJ,Silva LDC,Soares CMA,Andrade CH,Pereira M

    更新日期:2019-06-12 00:00:00

  • Impact of RNase E and RNase J on Global mRNA Metabolism in the Cyanobacterium Synechocystis PCC6803.

    abstract::mRNA levels result from an equilibrium between transcription and degradation. Ribonucleases (RNases) facilitate the turnover of mRNA, which is an important way of controlling gene expression, allowing the cells to adjust transcript levels to a changing environment. In contrast to the heterotrophic model bacteria Esche...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2020.01055

    authors: Cavaiuolo M,Chagneau C,Laalami S,Putzer H

    更新日期:2020-06-03 00:00:00

  • Nutrient Stoichiometry Shapes Microbial Community Structure in an Evaporitic Shallow Pond.

    abstract::Nutrient availability and ratios can play an important role in shaping microbial communities of freshwater ecosystems. The Cuatro Ciénegas Basin (CCB) in Mexico is a desert oasis where, perhaps paradoxically, high microbial diversity coincides with extreme oligotrophy. To better understand the effects of nutrients on ...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2017.00949

    authors: Lee ZM,Poret-Peterson AT,Siefert JL,Kaul D,Moustafa A,Allen AE,Dupont CL,Eguiarte LE,Souza V,Elser JJ

    更新日期:2017-05-30 00:00:00

  • Whole Genome Sequencing of Mycobacterium tuberculosis Clinical Isolates From India Reveals Genetic Heterogeneity and Region-Specific Variations That Might Affect Drug Susceptibility.

    abstract::Whole genome sequencing (WGS) of Mycobacterium tuberculosis has been constructive in understanding its evolution, genetic diversity and the mechanisms involved in drug resistance. A large number of sequencing efforts from across the globe have revealed genetic diversity among clinical isolates and the genetic determin...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2019.00309

    authors: Advani J,Verma R,Chatterjee O,Pachouri PK,Upadhyay P,Singh R,Yadav J,Naaz F,Ravikumar R,Buggi S,Suar M,Gupta UD,Pandey A,Chauhan DS,Tripathy SP,Gowda H,Prasad TSK

    更新日期:2019-02-26 00:00:00

  • Secondary Bacterial Infections Associated with Influenza Pandemics.

    abstract::Lower and upper respiratory infections are the fourth highest cause of global mortality (Lozano et al., 2012). Epidemic and pandemic outbreaks of respiratory infection are a major medical concern, often causing considerable disease and a high death toll, typically over a relatively short period of time. Influenza is a...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章,评审

    doi:10.3389/fmicb.2017.01041

    authors: Morris DE,Cleary DW,Clarke SC

    更新日期:2017-06-23 00:00:00

  • Transcriptional Regulation of the Type VI Secretion System 1 Genes by Quorum Sensing and ToxR in Vibrio parahaemolyticus.

    abstract::Vibrio parahaemolyticus, the leading cause of seafood-associated gastroenteritis, harbors two separate T6SSs on chromosomes 1 and 2, i.e., T6SS1 (VP1386-1420) and T6SS2 (VPA1025-1046). T6SS1 contains at least 7 putative operons: VP1386-1387, VP1388-1390, VP1392-1391, VP1393-1406, VP1400-1406, VP1409-1407, and VP1410-1...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2017.02005

    authors: Zhang Y,Gao H,Osei-Adjei G,Zhang Y,Yang W,Yang H,Yin Z,Huang X,Zhou D

    更新日期:2017-10-16 00:00:00

  • Next-Generation Beneficial Microbes: The Case of Akkermansia muciniphila.

    abstract::Metabolic disorders associated with obesity and cardiometabolic disorders are worldwide epidemic. Among the different environmental factors, the gut microbiota is now considered as a key player interfering with energy metabolism and host susceptibility to several non-communicable diseases. Among the next-generation be...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章,评审

    doi:10.3389/fmicb.2017.01765

    authors: Cani PD,de Vos WM

    更新日期:2017-09-22 00:00:00