Abstract:
:Phage therapy requires libraries of well-characterized phages. Here we describe the generation of phage libraries for three target species: Escherichia coli, Pseudomonas aeruginosa, and Enterobacter cloacae. The basic phage characteristics on the isolation host, sequence analysis, growth properties, and host range and virulence on a number of contemporary clinical isolates are presented. This information is required before phages can be added to a phage library for potential human use or sharing between laboratories for use in compassionate use protocols in humans under eIND (emergency investigational new drug). Clinical scenarios in which these phages can potentially be used are discussed. The phages presented here are currently being characterized in animal models and are available for eINDs.
journal_name
Front Microbioljournal_title
Frontiers in microbiologyauthors
Gibson SB,Green SI,Liu CG,Salazar KC,Clark JR,Terwilliger AL,Kaplan HB,Maresso AW,Trautner BW,Ramig RFdoi
10.3389/fmicb.2019.02537subject
Has Abstractpub_date
2019-11-12 00:00:00pages
2537issn
1664-302Xjournal_volume
10pub_type
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abstract::The thermophilic archaeon Sulfolobus acidocaldarius can use different carbon sources for growth, including the pentoses D-xylose and L-arabinose. In this study, we identified the activator XylR (saci_2116) responsible for the transcriptional regulation of the pentose transporter and pentose metabolizing genes in S. ac...
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abstract::Cefoperazone/sulbactam has been shown to be efficacious for the treatment of infections caused by Acinetobacter baumannii; however, the mechanism underlying resistance to this synergistic combination is not well understood. In the present study, two A. baumannii isolates, AB1845 and AB2092, were isolated from a patien...
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abstract::[This corrects the article DOI: 10.3389/fmicb.2019.02612.]. ...
journal_title:Frontiers in microbiology
pub_type: 已发布勘误
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abstract::Campylobacter is one of the most commonly reported foodborne human bacterial gastrointestinal pathogens. Campylobacter is the etiological agent of campylobacteriosis, which is generally a self-limited illness and therefore does not require treatment. However, when patients are immunocompromised or have other co-morbid...
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pub_type: 杂志文章,评审
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abstract::Losses in crop yields due to disease need to be reduced in order to meet increasing global food demands associated with growth in the human population. There is a well-recognized need to develop new environmentally friendly control strategies to combat bacterial crop disease. Current control measures involving the use...
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pub_type: 杂志文章,评审
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journal_title:Frontiers in microbiology
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abstract::This research uses inteins, a type of mobile genetic element, to infer patterns of gene transfer within the Halobacteria. We surveyed 118 genomes representing 26 genera of Halobacteria for intein sequences. We then used the presence-absence profile, sequence similarity and phylogenies from the inteins recovered to exp...
journal_title:Frontiers in microbiology
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journal_title:Frontiers in microbiology
pub_type: 杂志文章
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journal_title:Frontiers in microbiology
pub_type: 杂志文章
doi:10.3389/fmicb.2019.01592
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