Pseudomonas aeruginosa Swarmer Cells Adaptation Toward UVc Radiations.

Abstract:

:Swarming is the most rapid surface motility allowing Pseudomonas aeruginosa bacteria to rapidly colonize new surfaces. However, swarming behavior is affected by environmental factors like ultraviolet irradiation (UVc). UVc radiation is the most disinfection technology usually applied for wastewater and proven to be effective to inactivate microorganisms. However, efficiency against motile bacteria is not yet studied. This study aims to explain the mechanisms of resistance of swarmer P. aeruginosa cells toward UVc exposure. P. aeruginosa liquid cultures were allowed to swarm across a semisolid surface for 18 h and directly exposed to UVc radiations. Emergent swarmer colonies, revealed after re-incubation, were selected to study biofilm formation, fatty acid (FA) composition, and ultrastructure. Our results showed that membrane adaptation to UVc radiations was seen in Pseudomonas cells by an increase of cyclic fatty acid (CFA) content, confirming the role of cyclopropane in radio-resistance of swarmer cells. Furthermore, electron microscopic study confirmed that over production of S-layer is believed to be a protective form adopted by P. aeruginosa swarmer cells to resist after 5 min of UVc exposure. Moreover, membrane disintegration is the lethal effect observed after 15 min of UVc exposure. In the other hand, study of biofilm production showed an enhancement of biofilm formation, of swarmer cells mainly after 15 min of UVc exposure. There results confirmed that swarming process is highly correlated with particular FA composition of P. aeruginosa membrane and that radio-resistance of swarmer cells is highly supported by CFA biosynthesis and S-layer overproduction.

journal_name

Front Microbiol

authors

Ghorbal SKB,Chourabi K,Maalej L,Ammar AB,Ouzari HI,Hassen A,Jaafoura H,Chatti A

doi

10.3389/fmicb.2019.00556

subject

Has Abstract

pub_date

2019-04-02 00:00:00

pages

556

issn

1664-302X

journal_volume

10

pub_type

杂志文章
  • Regulation of the ER Stress Response by the Ion Channel Activity of the Infectious Bronchitis Coronavirus Envelope Protein Modulates Virion Release, Apoptosis, Viral Fitness, and Pathogenesis.

    abstract::Coronavirus (CoV) envelope (E) protein is a small structural protein critical for virion morphogenesis and release. The recently characterized E protein ion channel activity (EIC) has also been implicated in modulating viral pathogenesis. In this study, we used infectious bronchitis coronavirus (IBV) as a model to stu...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2019.03022

    authors: Li S,Yuan L,Dai G,Chen RA,Liu DX,Fung TS

    更新日期:2020-01-24 00:00:00

  • Iron - A Key Nexus in the Virulence of Aspergillus fumigatus.

    abstract::Iron is an essential but, in excess, toxic nutrient. Therefore, fungi evolved fine-tuned mechanisms for uptake and storage of iron, such as the production of siderophores (low-molecular mass iron-specific chelators). In Aspergillus fumigatus, iron starvation causes extensive transcriptional remodeling involving two ce...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2012.00028

    authors: Haas H

    更新日期:2012-02-06 00:00:00

  • Genomic and Functional Characterization of Enterococcus mundtii QAUEM2808, Isolated From Artisanal Fermented Milk Product Dahi.

    abstract::Microbial strains with a unique combination of technological and bioactive properties are preferred for industrial applications. The present study was conducted to evaluate the potential use of Enterococcus mundtii QAUEM2808 (NCBI Accession Number: LSMC00000000) in milk fermentation. This strain was isolated from Dahi...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2019.00434

    authors: Nawaz F,Khan MN,Javed A,Ahmed I,Ali N,Ali MI,Bakhtiar SM,Imran M

    更新日期:2019-03-26 00:00:00

  • Identification of Novel Long Non-coding and Circular RNAs in Human Papillomavirus-Mediated Cervical Cancer.

    abstract::Cervical cancer is the third most common cancer worldwide and the fourth leading cause of cancer-associated mortality in women. Accumulating evidence indicates that long non-coding RNAs (lncRNAs) and circular RNAs (circRNAs) may play key roles in the carcinogenesis of different cancers; however, little is known about ...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2017.01720

    authors: Wang H,Zhao Y,Chen M,Cui J

    更新日期:2017-09-19 00:00:00

  • Effect of Dietary Forage to Concentrate Ratios on Dynamic Profile Changes and Interactions of Ruminal Microbiota and Metabolites in Holstein Heifers.

    abstract::A better understanding of global ruminal microbiota and metabolites under extensive feeding conditions is a prerequisite for optimizing rumen function and improving ruminant feed efficiency. Furthermore, the gap between the information on the ruminal microbiota and metabolites needs to be bridged. The aim of this stud...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2017.02206

    authors: Zhang J,Shi H,Wang Y,Li S,Cao Z,Ji S,He Y,Zhang H

    更新日期:2017-11-09 00:00:00

  • Corrigendum: Lactobacillus Dominate in the Intestine of Atlantic Salmon Fed Dietary Probiotics.

    abstract::[This corrects the article DOI: 10.3389/fmicb.2018.03247.]. ...

    journal_title:Frontiers in microbiology

    pub_type: 已发布勘误

    doi:10.3389/fmicb.2019.01094

    authors: Gupta S,Fečkaninová A,Lokesh J,Koščová J,Sørensen M,Fernandes J,Kiron V

    更新日期:2019-05-15 00:00:00

  • Efficiency of Airborne Sample Analysis Platform (ASAP) bioaerosol sampler for pathogen detection.

    abstract::The threat of bioterrorism and pandemics has highlighted the urgency for rapid and reliable bioaerosol detection in different environments. Safeguarding against such threats requires continuous sampling of the ambient air for pathogen detection. In this study we investigated the efficacy of the Airborne Sample Analysi...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2015.00512

    authors: Sharma A,Clark E,McGlothlin JD,Mittal SK

    更新日期:2015-05-27 00:00:00

  • Vertical transmission explains the specific Burkholderia pattern in Sphagnum mosses at multi-geographic scale.

    abstract::The betaproteobacterial genus Burkholderia is known for its versatile interactions with its hosts that can range from beneficial to pathogenic. A plant-beneficial-environmental (PBE) Burkholderia cluster was recently separated from the pathogen cluster, yet still little is known about burkholderial diversity, distribu...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2013.00394

    authors: Bragina A,Cardinale M,Berg C,Berg G

    更新日期:2013-12-18 00:00:00

  • Plasmodium attenuation: connecting the dots between early immune responses and malaria disease severity.

    abstract::Sterile attenuation of Plasmodium parasites at the liver-stage either by irradiation or genetic modification, or at the blood-stage by chemoprophylaxis, has been shown to induce immune responses that can protect against subsequent wild-type infection. However, following certain interventions, parasite attenuation can ...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2014.00658

    authors: Fernandes P,Frank R,Lewis MD,Mueller AK

    更新日期:2014-12-02 00:00:00

  • Tet(C) Gene Transfer between Chlamydia suis Strains Occurs by Homologous Recombination after Co-infection: Implications for Spread of Tetracycline-Resistance among Chlamydiaceae.

    abstract::Chlamydia suis is a swine pathogen that has also recently been found to cause zoonotic infections of the human eye, pharynx, and gastrointestinal tract. Many strains contain a tetracycline class C gene [tet(C)] cassette that confers tetracycline resistance. The cassette was likely originally acquired by horizontal gen...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2017.00156

    authors: Marti H,Kim H,Joseph SJ,Dojiri S,Read TD,Dean D

    更新日期:2017-02-07 00:00:00

  • Global Proteomic Analysis of the Resuscitation State of Vibrio parahaemolyticus Compared With the Normal and Viable but Non-culturable State.

    abstract::Vibrio parahaemolyticus is a common pathogen which has become a major concern of seafood products. The bacteria in the viable but non-culturable (VBNC) state are unable to form colonies on growth media, but under appropriate conditions they can regain culturability. In this study, V. parahaemolyticus was induced into ...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2019.01045

    authors: Zhong Q,Wang B,Wang J,Liu Y,Fang X,Liao Z

    更新日期:2019-05-08 00:00:00

  • Influenza Virus Infection Induces Host Pyruvate Kinase M Which Interacts with Viral RNA-Dependent RNA Polymerase.

    abstract::Influenza virus RNA-dependent RNA polymerase (RdRp) is a heterotrimer of three viral proteins, PB1, PB2, and PA and is involved in both transcription and replication of the negative strand of the viral RNA (vRNA) genome. RdRp is multifunctional, possessing RNA polymerase, cap binding, and endonuclease activities. The ...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2017.00162

    authors: Miyake Y,Ishii K,Honda A

    更新日期:2017-02-09 00:00:00

  • Colistin Resistance Mediated by mcr-1 in ESBL-Producing, Multidrug Resistant Salmonella Infantis in Broiler Chicken Industry, Italy (2016-2017).

    abstract::Colistin-resistance mediated by mobilisable and plasmid-borne mcr genes has emerged worldwide, threatening the efficacy of colistin, a last resort antibiotic increasingly used for treating human invasive infections by multidrug-resistant or extensively drug-resistant Enterobacteriaceae. In this study, we report the fi...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2018.01880

    authors: Carfora V,Alba P,Leekitcharoenphon P,Ballarò D,Cordaro G,Di Matteo P,Donati V,Ianzano A,Iurescia M,Stravino F,Tagliaferri T,Battisti A,Franco A

    更新日期:2018-08-17 00:00:00

  • Nanotechnology in Sustainable Agriculture: Recent Developments, Challenges, and Perspectives.

    abstract::Nanotechnology monitors a leading agricultural controlling process, especially by its miniature dimension. Additionally, many potential benefits such as enhancement of food quality and safety, reduction of agricultural inputs, enrichment of absorbing nanoscale nutrients from the soil, etc. allow the application of nan...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章,评审

    doi:10.3389/fmicb.2017.01014

    authors: Prasad R,Bhattacharyya A,Nguyen QD

    更新日期:2017-06-20 00:00:00

  • Bio-Decolorization of Synthetic Dyes by a Halophilic Bacterium Salinivibrio sp.

    abstract::Synthetic dyes, extensively used in various industries, act as pollutants in the aquatic environment, and pose a significant threat to living beings. In the present study, we assessed the potential of a halophilic bacterium Salinivibrio kushneri HTSP isolated from a saltpan for decolorization and bioremediation of syn...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2020.594011

    authors: John J,Dineshram R,Hemalatha KR,Dhassiah MP,Gopal D,Kumar A

    更新日期:2020-12-21 00:00:00

  • Repurposed Antimicrobial Combination Therapy: Tobramycin-Ciprofloxacin Hybrid Augments Activity of the Anticancer Drug Mitomycin C Against Multidrug-Resistant Gram-Negative Bacteria.

    abstract::The lack of therapeutic options to treat infections caused by multidrug-resistant (MDR) pathogens, especially Gram-negative bacteria, is apparent. Therefore, it is imperative to develop new strategies to address the problem of antimicrobial resistance. Repurposing non-antibiotic commercial drugs for antimicrobial ther...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2019.01556

    authors: Domalaon R,Ammeter D,Brizuela M,Gorityala BK,Zhanel GG,Schweizer F

    更新日期:2019-07-10 00:00:00

  • Swine Enteric Colibacillosis in Spain: Pathogenic Potential of mcr-1 ST10 and ST131 E. coli Isolates.

    abstract::This is a wide epidemiological study of 499 E. coli isolates recovered from 179 outbreaks of enteric colibacillosis from pig production farms in Spain during a period of 10 years. Most samples were of diarrheagenic cases occurred during the post-wean period (PWD) which showed to be significantly associated with ETEC (...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2018.02659

    authors: García-Meniño I,García V,Mora A,Díaz-Jiménez D,Flament-Simon SC,Alonso MP,Blanco JE,Blanco M,Blanco J

    更新日期:2018-11-05 00:00:00

  • The molecular dimension of microbial species: 1. Ecological distinctions among, and homogeneity within, putative ecotypes of Synechococcus inhabiting the cyanobacterial mat of Mushroom Spring, Yellowstone National Park.

    abstract::Based on the Stable Ecotype Model, evolution leads to the divergence of ecologically distinct populations (e.g., with different niches and/or behaviors) of ecologically interchangeable membership. In this study, pyrosequencing was used to provide deep sequence coverage of Synechococcus psaA genes and transcripts over ...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2015.00590

    authors: Becraft ED,Wood JM,Rusch DB,Kühl M,Jensen SI,Bryant DA,Roberts DW,Cohan FM,Ward DM

    更新日期:2015-06-22 00:00:00

  • Bacillus cereus cell response upon exposure to acid environment: toward the identification of potential biomarkers.

    abstract::Microorganisms are able to adapt to different environments and evolve rapidly, allowing them to cope with their new environments. Such adaptive response and associated protections toward other lethal stresses, is a crucial survival strategy for a wide spectrum of microorganisms, including food spoilage bacteria, patho...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章,评审

    doi:10.3389/fmicb.2013.00284

    authors: Desriac N,Broussolle V,Postollec F,Mathot AG,Sohier D,Coroller L,Leguerinel I

    更新日期:2013-10-02 00:00:00

  • Klebsiella Phage KP34 RNA Polymerase and Its Use in RNA Synthesis.

    abstract::We have characterized the single subunit RNA polymerase from Klebsiella phage KP34. The enzyme is unique among known bacteriophage RNA polymerases in that it recognizes two unrelated promoter sequences, which provided clues for the evolution of phage single-subunit RNA polymerases. As the first representative enzyme f...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2019.02487

    authors: Lu X,Wu H,Xia H,Huang F,Yan Y,Yu B,Cheng R,Drulis-Kawa Z,Zhu B

    更新日期:2019-10-31 00:00:00

  • The Bacteriophage Carrier State of Campylobacter jejuni Features Changes in Host Non-coding RNAs and the Acquisition of New Host-derived CRISPR Spacer Sequences.

    abstract::Incorporation of self-derived CRISPR DNA protospacers in Campylobacter jejuni PT14 occurs in the presence of bacteriophages encoding a CRISPR-like Cas4 protein. This phenomenon was evident in carrier state infections where both bacteriophages and host are maintained for seemingly indefinite periods as stable populatio...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章,评审

    doi:10.3389/fmicb.2016.00355

    authors: Hooton SP,Brathwaite KJ,Connerton IF

    更新日期:2016-03-23 00:00:00

  • Importance of Promyelocytic Leukema Protein (PML) for Kaposi's Sarcoma-Associated Herpesvirus Lytic Replication.

    abstract::Many DNA virus replication-related proteins are associated with promyelocytic leukemia protein (PML), a component of nuclear domain 10 (ND10), which has been investigated for its potential involvement in viral replication. In the case of Kaposi's sarcoma-associated herpesvirus (KSHV) lytic gene products, K8 (K-bZIP), ...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2018.02324

    authors: Hossain MG,Ohsaki E,Honda T,Ueda K

    更新日期:2018-10-08 00:00:00

  • Effects of Wilting and Lactobacillus plantarum Addition on the Fermentation Quality and Microbial Community of Moringa oleifera Leaf Silage.

    abstract::The objective of this study was to evaluate the effects of wilting and Lactobacillus plantarum (LP) addition on the silage fermentation quality and microbial community of Moringa oleifera Lam. leaf silage. Unwilted (direct-cut) or wilted M. oleifera leaves were prepared either with or without LP (1.0 × 106 cfu/g) foll...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2018.01817

    authors: Wang Y,Wang C,Zhou W,Yang FY,Chen XY,Zhang Q

    更新日期:2018-08-06 00:00:00

  • An Essential Factor for High Mg2+ Tolerance of Staphylococcus aureus.

    abstract::Internal bacterial concentration of Mg2+, the most abundant divalent cation in living cells, is estimated to be in the single millimolar range. However, many bacteria will thrive in media with only micromolars of Mg2+, by using a range of intensely studied and highly efficient import mechanisms, as well as in media wi...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2016.01888

    authors: Armitano J,Redder P,Guimarães VA,Linder P

    更新日期:2016-11-25 00:00:00

  • Isolation, Identification, and Complete Genome Assembly of an Endophytic Bacillus velezensis YB-130, Potential Biocontrol Agent Against Fusarium graminearum.

    abstract::Wheat scab caused by F. graminearum is a highly destructive disease that leads to yield reduction and mycotoxin contamination of grains. In this study, an endophytic bacterium of strain YB-130 was isolated from surface sterilized wheat spikes with scab symptoms and identified as Bacillus velezensis by whole genome ann...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2020.598285

    authors: Xu W,Zhang L,Goodwin PH,Xia M,Zhang J,Wang Q,Liang J,Sun R,Wu C,Yang L

    更新日期:2020-12-03 00:00:00

  • A Pilot Study of the Humoral Response Against the AntiSense Protein (ASP) in HIV-1-Infected Patients.

    abstract::The existence of an antisense Open Reading Frame (ORF) that encodes a putative AntiSense Protein (ASP) on the proviral genome of Human Immunodeficiency Virus type 1 (HIV-1) was a source of debate for 30 years. During the last years, some progresses have been made to characterize the cellular immune response against AS...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2020.00020

    authors: Savoret J,Chazal N,Moles JP,Tuaillon E,Boufassa F,Meyer L,Lecuroux C,Lambotte O,Van De Perre P,Mesnard JM,Gross A

    更新日期:2020-01-24 00:00:00

  • A Burkholderia thailandensis DedA Family Membrane Protein Is Required for Proton Motive Force Dependent Lipid A Modification.

    abstract::The DedA family is a conserved membrane protein family found in most organisms. A Burkholderia thailandensis DedA family protein, named DbcA, is required for high-level colistin (polymyxin E) resistance, but the mechanism awaits elucidation. Modification of lipopolysaccharide lipid A with the cationic sugar aminoarabi...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2020.618389

    authors: Panta PR,Doerrler WT

    更新日期:2021-01-12 00:00:00

  • Genetic and Toxigenic Variability within Aspergillus flavus Population Isolated from Maize in Two Diverse Environments in Kenya.

    abstract::Aspergillus flavus is the main producer of carcinogenic aflatoxins in agricultural commodities such as maize. This fungus occurs naturally on crops, and produces aflatoxins when environmental conditions are favorable. The aim of this study is to analyse the genetic variability among 109 A. flavus isolates previously r...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2018.00057

    authors: Okoth S,De Boevre M,Vidal A,Diana Di Mavungu J,Landschoot S,Kyallo M,Njuguna J,Harvey J,De Saeger S

    更新日期:2018-01-26 00:00:00

  • Study of the cwaRS-ldcA Operon Coding a Two-Component System and a Putative L,D-Carboxypeptidase in Lactobacillus paracasei.

    abstract::The cell surface is the primary recognition site between the bacterium and the host. An operon of three genes, LSEI_0219 (cwaR), LSEI_0220 (cwaS), and LSEI_0221 (ldcA), has been previously identified as required for the establishment of Lactobacillus paracasei in the gut. The genes cwaR and cwaS encode a predicted two...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2020.00156

    authors: Scornec H,Palud A,Pédron T,Wheeler R,Petitgonnet C,Boneca IG,Cavin JF,Sansonetti PJ,Licandro H

    更新日期:2020-03-03 00:00:00

  • Plant and Microbial Responses to Repeated Cu(OH)2 Nanopesticide Exposures Under Different Fertilization Levels in an Agro-Ecosystem.

    abstract::The environmental fate and potential impacts of nanopesticides on agroecosystems under realistic agricultural conditions are poorly understood. As a result, the benefits and risks of these novel formulations compared to the conventional products are currently unclear. Here, we examined the effects of repeated realisti...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2018.01769

    authors: Simonin M,Colman BP,Tang W,Judy JD,Anderson SM,Bergemann CM,Rocca JD,Unrine JM,Cassar N,Bernhardt ES

    更新日期:2018-07-31 00:00:00