当前位置： SCI文献检索 > Frontiers in Microbiology期刊下所有文献 > 13C-DNA-SIP Distinguishes the Prokaryotic Community That Metabolizes Soybean Residues Produced Under Different CO2 Concentrations.

13C-DNA-SIP Distinguishes the Prokaryotic Community That Metabolizes Soybean Residues Produced Under Different CO2 Concentrations.

Abstract：

:The amendment of crop residues produced under elevated CO2 (eCO2) may alter soil microbial community structure and their functions on residue decomposition and carbon (C) cycling in soil. The key to understanding this process is to elucidate the structure of prokaryotic communities that metabolize crop residues derived from eCO2. A soil incubation experiment was conducted to explore the response of soil microbial community to the amendment of 13C-labeled soybean residues produced under ambient CO2 (aCO2) and eCO2. The residues were applied to a Mollisol, followed by 13C-DNA stable isotope probing (SIP) and Illumina sequencing on soil prokaryotic community over time. The structure of residue-metabolizing community differed in response to the amendment of eCO2- and aCO2-derived residues after 28 days of incubation. In particular, genera Actinomadura, Nocardia, Non-omuraea, and Shimazuella were the dominant members of the residue-metabolizing bacteria, which contributed to this difference. The relative abundances of genera Actinomadura, Nocardia and Shimazuella were 118-144%, 71-113%, and 2-4-fold higher in the Mollisol amended with aCO2-derived than eCO2-derived residue. In contrast, the relative abundance of Non-omuraea was 87-90% greater in the eCO2-residue treatment. However, during the incubation period, there was no difference between the two residue treatments in the community structure as a whole without SIP. These results implied that a pioneering prokaryotic community metabolized the residue initially prior to the entire community. Those bacteria genera being inhibited with the amendment of the eCO2-derived residue, compared to aCO2-derived residue, were likely preferential to metabolize recalcitrant C, which might be associated with changes of chemical composition of the residue under eCO2.

journal_name

Front Microbiol

journal_title

Frontiers in microbiology

authors

Wang Y,Yu Z,Li Y,Wang G,Tang C,Liu X,Liu J,Xie Z,Jin J

doi

10.3389/fmicb.2019.02184

subject

Has Abstract

pub_date

2019-09-24 00:00:00

pages

2184

issn

1664-302X

journal_volume

pub_type

杂志文章

在线工具

13C-DNA-SIP Distinguishes the Prokaryotic Community That Metabolizes Soybean Residues Produced Under Different CO2 Concentrations.