Specific in vitro interferon-gamma and IL-2 production as biomarkers during treatment of chronic Q fever.

Abstract:

BACKGROUND:Antibiotic treatment of chronic Q fever is cumbersome and of long duration. To monitor treatment, there is a need for alternative biomarkers. Coxiella burnetii-specific interferon (IFN)-γ and interleukin (IL)-2 production reflect the type of effector and memory T-cell response. In chronic Q fever, C. burnetii-specific IFN-γ production is higher and IL-2 production is lower than in individuals with past Q fever. Here we explore whether C. burnetii-specific IFN-γ and IL-2 production correlate to treatment response. METHODS:We studied the longitudinal C. burnetii-specific IFN-γ/IL-2 ratio in fifteen proven chronic Q fever patients. All patients were followed for at least 18 months during antibiotic treatment. Treatment was considered successful when clinical recovery was observed, a positive PCR for C. burnetii DNA in blood became persistently negative, anti-phase I IgG showed a fourfold decrease or more, and imaging techniques showed disappearance of infectious foci. RESULTS:Overall, the IFN-γ/IL-2 ratio declined when patients experienced a successful treatment outcome. When treatment failed, IFN-γ/IL-2 ratios did not significantly decrease. The median (±IQR) slope of the longitudinal IFN-γ/IL-2 ratio with successful treatment was -2.10 (-7.02 to -0.06), and -0.15 (-1.13 to 0.25) with unsuccessful treatment (P = 0.19). Q fever endocarditis patients had higher IFN-γ/IL-2 ratios than patients with endovascular infections. CONCLUSION:We propose that the IFN-γ/IL-2 ratio can be used as an additional biomarker for monitoring chronic Q fever treatment, with declining ratios being indicative of successful treatment.

journal_name

Front Microbiol

authors

Schoffelen T,Wegdam-Blans MC,Ammerdorffer A,Pronk MJ,Soethoudt YE,Netea MG,van der Meer JW,Bleeker-Rovers CP,van Deuren M

doi

10.3389/fmicb.2015.00093

subject

Has Abstract

pub_date

2015-02-12 00:00:00

pages

93

issn

1664-302X

journal_volume

6

pub_type

杂志文章
  • Relating Phylogenetic and Functional Diversity among Denitrifiers and Quantifying their Capacity to Predict Community Functioning.

    abstract::Genetic diversity of phylogenetic or functional markers is widely used as a proxy of microbial diversity. However, it remains unclear to what extent functional diversity (FD), gene sequence diversity and community functioning are linked. For a range of denitrifying bacteria, we analyzed the relationships between (i) t...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2012.00209

    authors: Salles JF,Le Roux X,Poly F

    更新日期:2012-06-12 00:00:00

  • Dissection of the cis-2-decenoic acid signaling network in Pseudomonas aeruginosa using microarray technique.

    abstract::Many bacterial pathogens use quorum-sensing (QS) signaling to regulate the expression of factors contributing to virulence and persistence. Bacteria produce signals of different chemical classes. The signal molecule, known as diffusible signal factor (DSF), is a cis-unsaturated fatty acid that was first described in t...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2015.00383

    authors: Rahmani-Badi A,Sepehr S,Fallahi H,Heidari-Keshel S

    更新日期:2015-04-28 00:00:00

  • Morphologically Distinct Escherichia coli Bacteriophages Differ in Their Efficacy and Ability to Stimulate Cytokine Release In Vitro.

    abstract::Due to a global increase in the range and number of infections caused by multi-resistant bacteria, phage therapy is currently experiencing a resurgence of interest. However, there are a number of well-known concerns over the use of phages to treat bacterial infections. In order to address concerns over safety and the ...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2016.00437

    authors: Khan Mirzaei M,Haileselassie Y,Navis M,Cooper C,Sverremark-Ekström E,Nilsson AS

    更新日期:2016-03-31 00:00:00

  • YtrASa, a GntR-Family Transcription Factor, Represses Two Genetic Loci Encoding Membrane Proteins in Sulfolobus acidocaldarius.

    abstract::In bacteria, the GntR family is a widespread family of transcription factors responsible for the regulation of a myriad of biological processes. In contrast, despite their occurrence in archaea only a little information is available on the function of GntR-like transcription factors in this domain of life. The thermoa...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2019.02084

    authors: Lemmens L,Tilleman L,De Koning E,Valegård K,Lindås AC,Van Nieuwerburgh F,Maes D,Peeters E

    更新日期:2019-09-10 00:00:00

  • NAuRA: Genomic Tool to Identify Staphylococcal Enterotoxins in Staphylococcus aureus Strains Responsible for FoodBorne Outbreaks.

    abstract::Food contamination by staphylococcal enterotoxins (SEs) is responsible for many food poisoning outbreaks (FPOs) each year, and they represent the third leading cause of FPOs in Europe. SEs constitute a protein family with 27 proteins. However, enzyme immunoassays can only detect directly in food the five classical SEs...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2020.01483

    authors: Merda D,Felten A,Vingadassalon N,Denayer S,Titouche Y,Decastelli L,Hickey B,Kourtis C,Daskalov H,Mistou MY,Hennekinne JA

    更新日期:2020-06-30 00:00:00

  • Development of Multiple Cross Displacement Amplification Label-Based Gold Nanoparticles Lateral Flow Biosensor for Detection of Shigella spp.

    abstract::Shigella spp., the etiological agent of shigellosis or "bacillary dysentery," are responsible for considerable morbidity and mortality in excess of a million deaths globally per year. Although PCR-based techniques (such as PCR-based dipstick biosensors) have been used for the molecular diagnosis of infectious disease,...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2016.01834

    authors: Wang Y,Wang Y,Xu J,Ye C

    更新日期:2016-11-18 00:00:00

  • Source Tracking Based on Core Genome SNV and CRISPR Typing of Salmonella enterica Serovar Heidelberg Isolates Involved in Foodborne Outbreaks in Québec, 2012.

    abstract::Whole-genome sequencing (WGS) is the method of choice for bacterial subtyping and it is rapidly replacing the more traditional methods such as pulsed-field gel electrophoresis (PFGE). Here we used the high-resolution core genome single nucleotide variant (cgSNV) typing method to characterize clinical and food from Sal...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2020.01317

    authors: Yousfi K,Usongo V,Berry C,Khan RH,Tremblay DM,Moineau S,Mulvey MR,Doualla-Bell F,Fournier E,Nadon C,Goodridge L,Bekal S

    更新日期:2020-06-17 00:00:00

  • Dynamics of Insect-Microbiome Interaction Influence Host and Microbial Symbiont.

    abstract::Insects share an intimate relationship with their gut microflora and this symbiotic association has developed into an essential evolutionary outcome intended for their survival through extreme environmental conditions. While it has been clearly established that insects, with very few exceptions, associate with several...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章,评审

    doi:10.3389/fmicb.2020.01357

    authors: Gupta A,Nair S

    更新日期:2020-06-26 00:00:00

  • Heterologous Production of a Novel Cyclic Peptide Compound, KK-1, in Aspergillus oryzae.

    abstract::A novel cyclic peptide compound, KK-1, was originally isolated from the plant-pathogenic fungus Curvularia clavata. It consists of 10 amino acid residues, including five N-methylated amino acid residues, and has potent antifungal activity. Recently, the genome-sequencing analysis of C. clavata was completed, and the b...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2018.00690

    authors: Yoshimi A,Yamaguchi S,Fujioka T,Kawai K,Gomi K,Machida M,Abe K

    更新日期:2018-04-09 00:00:00

  • Serotype Distribution and Antimicrobial Susceptibility of Streptococcus pneumoniae in Pre- and Post- PCV7/13 Eras, Taiwan, 2002-2018.

    abstract::In Taiwan, the 7-valent pneumococcal conjugate vaccine (PCV7) was introduced in 2006 and a PCV13 national childhood catchup program was implemented in 2013. To delineate the trend of serotype distribution and antimicrobial susceptibility following vaccination programs, we investigated a total of 1845 Streptococcus pne...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2020.557404

    authors: Wu CJ,Lai JF,Huang IW,Shiau YR,Wang HY,Lauderdale TL

    更新日期:2020-10-22 00:00:00

  • Comparative Evolutionary Patterns of Burkholderia cenocepacia and B. multivorans During Chronic Co-infection of a Cystic Fibrosis Patient Lung.

    abstract::During chronic respiratory infections of cystic fibrosis (CF) patients, bacteria adaptively evolve in response to the nutritional and immune environment as well as influence other infecting microbes. The present study was designed to gain insights into the genetic mechanisms underlying adaptation and diversification b...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2020.574626

    authors: Hassan AA,Dos Santos SC,Cooper VS,Sá-Correia I

    更新日期:2020-09-25 00:00:00

  • Aquaporin-4 Is Downregulated in the Basolateral Membrane of Ileum Epithelial Cells during Enterotoxigenic Escherichia coli-Induced Diarrhea in Mice.

    abstract::Enterotoxigenic Escherichia coli (ETEC) are opportunistic pathogens that colonize the small intestine, produce enterotoxins and induce diarrhea. Some aquaporins (AQPs), such as AQP3 and AQP8, have been reported to participate in diarrhea by decreasing cellular influx in the gastrointestinal (GI) tract. AQP4 is another...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2017.02655

    authors: Zhang D,Yang L,Su W,Zhao Y,Ma X,Zhou H,Xu B,Zhang K,Ma H

    更新日期:2018-01-09 00:00:00

  • Comparative Genomics of Completely Sequenced Lactobacillus helveticus Genomes Provides Insights into Strain-Specific Genes and Resolves Metagenomics Data Down to the Strain Level.

    abstract::Although complete genome sequences hold particular value for an accurate description of core genomes, the identification of strain-specific genes, and as the optimal basis for functional genomics studies, they are still largely underrepresented in public repositories. Based on an assessment of the genome assembly comp...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2018.00063

    authors: Schmid M,Muri J,Melidis D,Varadarajan AR,Somerville V,Wicki A,Moser A,Bourqui M,Wenzel C,Eugster-Meier E,Frey JE,Irmler S,Ahrens CH

    更新日期:2018-01-30 00:00:00

  • SesI May Be Associated with the Invasiveness of Staphylococcus epidermidis.

    abstract::Staphylococcus epidermidis is a commensal bacterium which widely colonizes in human skin and mucous membrane and rarely causes clinically manifested infections. S. epidermidis surface protein I (SesI) is considered to be the major virulence factor of S. epidermidis infection, but its pathogenesis is not clear. Here, w...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2017.02574

    authors: Qi X,Jin Y,Duan J,Hao Z,Wang S,Guo Y,Lv J,Hu L,Wang L,Yu F

    更新日期:2018-01-04 00:00:00

  • SMRT Sequencing Revealed Mitogenome Characteristics and Mitogenome-Wide DNA Modification Pattern in Ophiocordyceps sinensis.

    abstract::Single molecule, real-time (SMRT) sequencing was used to characterize mitochondrial (mt) genome of Ophiocordyceps sinensis and to analyze the mt genome-wide pattern of epigenetic DNA modification. The complete mt genome of O. sinensis, with a size of 157,539 bp, is the fourth largest Ascomycota mt genome sequenced to ...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2017.01422

    authors: Kang X,Hu L,Shen P,Li R,Liu D

    更新日期:2017-07-27 00:00:00

  • Analysis of the Rumen Microbiome and Metabolome to Study the Effect of an Antimethanogenic Treatment Applied in Early Life of Kid Goats.

    abstract::This work aimed to gain insight into the transition from milk to solid feeding at weaning combining genomics and metabolomics on rumen contents from goat kids treated with a methanogenic inhibitor (bromochloromethane, BCM). Sixteen goats giving birth to two kids were used. Eight does were treated (D+) with BCM after g...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2018.02227

    authors: Abecia L,Martínez-Fernandez G,Waddams K,Martín-García AI,Pinloche E,Creevey CJ,Denman SE,Newbold CJ,Yáñez-Ruiz DR

    更新日期:2018-10-09 00:00:00

  • Comparative Proteomics of Meat Spoilage Bacteria Predicts Drivers for Their Coexistence on Modified Atmosphere Packaged Meat.

    abstract::Besides intrinsic and extrinsic factors such as antagonism for organic substrates or temperature, the storage atmosphere of meat has a high influence on the development of its initial microbiota. Specific modified atmospheres (MAs) selectively suppress growth of aerobic and anaerobic bacteria, thus reshaping the initi...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2020.00209

    authors: Kolbeck S,Ludwig C,Meng C,Hilgarth M,Vogel RF

    更新日期:2020-02-14 00:00:00

  • xCT, not just an amino-acid transporter: a multi-functional regulator of microbial infection and associated diseases.

    abstract::Expression of xCT, a component of the xc (-) amino-acid transporter, is essential for the uptake of cystine required for intracellular glutathione (GSH) synthesis and maintenance of the intracellular redox balance. Therefore, xCT plays an important role not only in the survival of somatic and immune cells, but also in...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2015.00120

    authors: Dai L,Noverr MC,Parsons C,Kaleeba JA,Qin Z

    更新日期:2015-02-19 00:00:00

  • Viral Induced Microbial Mortality in Arctic Hypersaline Spring Sediments.

    abstract::Viruses are a primary influence on microbial mortality in the global ocean. The impacts of viruses on their microbial hosts in low-energy environments are poorly explored and are the focus of this study. To investigate the role of viruses in mediating mortality in low-energy environments where contacts between viruses...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2016.02158

    authors: Colangelo-Lillis J,Wing BA,Raymond-Bouchard I,Whyte LG

    更新日期:2017-01-23 00:00:00

  • Alpine Snow Algae Microbiome Diversity in the Coast Range of British Columbia.

    abstract::Snow algae blooms contain bacteria, fungi, and other microscopic organisms. We surveyed 55 alpine snow algae blooms, collecting a total of 68 samples, from 12 mountains in the Coast Range of British Columbia, Canada. We used microscopy and rDNA metabarcoding to document biodiversity and query species and taxonomic ass...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2020.01721

    authors: Yakimovich KM,Engstrom CB,Quarmby LM

    更新日期:2020-07-28 00:00:00

  • Helicobacter pylori Outer Membrane Vesicles Protect the Pathogen From Reactive Oxygen Species of the Respiratory Burst.

    abstract::Outer membrane vesicles (OMVs) play an important role in the persistence of Helicobacter pylori infection. Helicobacter OMVs carry a plethora of virulence factors, including catalase (KatA), an antioxidant enzyme that counteracts the host respiratory burst. We found KatA to be enriched and surface-associated in OMVs c...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2018.01837

    authors: Lekmeechai S,Su YC,Brant M,Alvarado-Kristensson M,Vallström A,Obi I,Arnqvist A,Riesbeck K

    更新日期:2018-09-07 00:00:00

  • Populations of Stored Product Mite Tyrophagus putrescentiae Differ in Their Bacterial Communities.

    abstract:BACKGROUND:Tyrophagus putrescentiae colonizes different human-related habitats and feeds on various post-harvest foods. The microbiota acquired by these mites can influence the nutritional plasticity in different populations. We compared the bacterial communities of five populations of T. putrescentiae and one mixed po...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2016.01046

    authors: Erban T,Klimov PB,Smrz J,Phillips TW,Nesvorna M,Kopecky J,Hubert J

    更新日期:2016-07-12 00:00:00

  • Evidence for horizontal gene transfer of anaerobic carbon monoxide dehydrogenases.

    abstract::Carbon monoxide (CO) is commonly known as a toxic gas, yet both cultivation studies and emerging genome sequences of bacteria and archaea establish that CO is a widely utilized microbial growth substrate. In this study, we determined the prevalence of anaerobic carbon monoxide dehydrogenases ([Ni,Fe]-CODHs) in current...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2012.00132

    authors: Techtmann SM,Lebedinsky AV,Colman AS,Sokolova TG,Woyke T,Goodwin L,Robb FT

    更新日期:2012-04-17 00:00:00

  • The Impact of the Antimicrobial Compounds Produced by Lactic Acid Bacteria on the Growth Performance of Mycobacterium avium subsp. paratuberculosis.

    abstract::Cell-free supernatants (CFSs) extracted from various lactic acid bacteria (LAB) cultures were applied to Mycobacterium avium subsp. paratuberculosis (MAP) cells to determine their effect on MAP viability. In addition, 5% lactic acid (LA; pH 3) and commercially synthetized nisin bacteriocin were also tested. This proce...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2018.00638

    authors: Kralik P,Babak V,Dziedzinska R

    更新日期:2018-04-03 00:00:00

  • Escherichia coli Cells Exposed to Lethal Doses of Electron Beam Irradiation Retain Their Ability to Propagate Bacteriophages and Are Metabolically Active.

    abstract::Reports in the literature suggest that bacteria exposed to lethal doses of ionizing radiation, i.e., electron beams, are unable to replicate yet they remain metabolically active. To investigate this phenomenon further, we electron beam irradiated Escherichia coli cells to a lethal dose and measured their membrane inte...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2018.02138

    authors: Hieke AC,Pillai SD

    更新日期:2018-09-10 00:00:00

  • No Evidence That Homologs of Key Circadian Clock Genes Direct Circadian Programs of Development or mRNA Abundance in Verticillium dahliae.

    abstract::Many organisms harbor circadian clocks that promote their adaptation to the rhythmic environment. While a broad knowledge of the molecular mechanism of circadian clocks has been gained through the fungal model Neurospora crassa, little is known about circadian clocks in other fungi. N. crassa belongs to the same class...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2020.01977

    authors: Cascant-Lopez E,Crosthwaite SK,Johnson LJ,Harrison RJ

    更新日期:2020-08-28 00:00:00

  • Design of Primers for Evaluation of Lactic Acid Bacteria Populations in Complex Biological Samples.

    abstract::Lactic acid bacteria (LAB) are important for human health. However, the relative abundance of LAB in complex samples, such as fecal samples, is low and their presence and diversity (at the species level) is understudied. Therefore, we designed LAB-specific primer pairs based on 16S rRNA gene consensus sequences from 4...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2018.02045

    authors: Hou Q,Bai X,Li W,Gao X,Zhang F,Sun Z,Zhang H

    更新日期:2018-08-31 00:00:00

  • Metabolomic and high-throughput sequencing analysis-modern approach for the assessment of biodeterioration of materials from historic buildings.

    abstract::Preservation of cultural heritage is of paramount importance worldwide. Microbial colonization of construction materials, such as wood, brick, mortar, and stone in historic buildings can lead to severe deterioration. The aim of the present study was to give modern insight into the phylogenetic diversity and activated ...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2015.00979

    authors: Gutarowska B,Celikkol-Aydin S,Bonifay V,Otlewska A,Aydin E,Oldham AL,Brauer JI,Duncan KE,Adamiak J,Sunner JA,Beech IB

    更新日期:2015-09-29 00:00:00

  • Functional Characterization of Two Putative DAHP Synthases of AroG1 and AroG2 and Their Links With Type III Secretion System in Ralstonia solanacearum.

    abstract::Type three secretion system (T3SS) is essential for Ralstonia solanacearum to cause disease in host plants and we previously screened AroG1 as a candidate with impact on the T3SS expression. Here, we focused on two putative DAHP synthases of AroG1 and AroG2, which control the first step of the shikimate pathway, a com...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2019.00183

    authors: Zhang W,Li J,Shi X,Hikichi Y,Zhang Y,Ohnishi K

    更新日期:2019-02-12 00:00:00

  • Diversity of laccase-coding genes in Fusarium oxysporum genomes.

    abstract::Multiple studies confirm laccase role in fungal pathogenicity and lignocellulose degradation. In spite of broad genomic research, laccases from plant wilt pathogen Fusarium oxysporum are still not characterized. The study aimed to identify F. oxysporum genes that may encode laccases sensu stricto and to characterize t...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2015.00933

    authors: Kwiatos N,Ryngajłło M,Bielecki S

    更新日期:2015-09-10 00:00:00