The Heat Shock Protein 70 Family of Chaperones Regulates All Phases of the Enterovirus A71 Life Cycle.

Abstract:

:Enterovirus A71 (EV-A71) is one of the major etiologic agents causing hand, foot, and mouth disease (HFMD) in children and occasionally causes severe neurological diseases or even death. EV-A71 replicates rapidly in host cells. For a successful infection, viruses produce large quantities of viral proteins in a short period, which requires cellular chaperone proteins for viral protein folding and viral particle assembly. In this study, we explored the roles of the heat shock protein 70 (HSP70) chaperone subnetwork in the EV-A71 life cycle. Our results revealed that EV-A71 exploits multiple HSP70s at each step of the viral life cycle, i.e., viral entry, translation, replication, assembly and release, and that each HSP70 typically functions in several stages of the life cycle. For example, the HSP70 isoforms HSPA1, HSPA8, and HSPA9 are required for viral entry and the translational steps of the infection. HSPA8 and HSPA9 may facilitate folding and stabilize viral proteins 3D and 2C, respectively, thus contributing to the formation of a replication complex. HSPA8 and HSPA9 also promote viral particle assembly, whereas HSPA1 and HSPA8 are involved in viral particle release. Because of the importance of various HSP70s at distinct steps of the viral life cycle, an allosteric inhibitor, JG40, which targets all HSP70s, significantly blocks EV-A71 infection. JG40 also blocks the replication of several other enteroviruses, such as coxsackievirus (CV) A16, CVB1, CVB3, and echovirus 11. Thus, targeting HSP70s may be a means of providing broad-spectrum antiviral therapy.

journal_name

Front Microbiol

authors

Su YS,Hsieh PY,Li JS,Pao YH,Chen CJ,Hwang LH

doi

10.3389/fmicb.2020.01656

subject

Has Abstract

pub_date

2020-07-14 00:00:00

pages

1656

issn

1664-302X

journal_volume

11

pub_type

杂志文章
  • PP2A-Like Protein Phosphatase (Sit4) Regulatory Subunits, Sap155 and Sap190, Regulate Candida albicans' Cell Growth, Morphogenesis, and Virulence.

    abstract::PP2A-like phosphatases share high homology with PP2A enzymes and are composed of a catalytic subunit and a regulatory subunit. In Candida albicans, the PP2A-like catalytic subunit SIT4 regulates cell growth, morphogenesis, and virulence. However, the functions of its regulatory subunits remain unclear. Here, by homolo...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2019.02943

    authors: Han Q,Pan C,Wang Y,Zhao L,Wang Y,Sang J

    更新日期:2019-12-20 00:00:00

  • Effect of climate change on Aspergillus flavus and aflatoxin B1 production.

    abstract::This review considers the available information on the potential impact of key environmental factors and their interactions on the molecular ecology, growth and aflatoxin production by Aspergillus flavus in vitro and in maize grain. The recent studies which have been carried out to examine the impact of water activity...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章,评审

    doi:10.3389/fmicb.2014.00348

    authors: Medina A,Rodriguez A,Magan N

    更新日期:2014-07-22 00:00:00

  • An Omics Perspective on Candida Infections: Toward Next-Generation Diagnosis and Therapy.

    abstract::Candida species can cause severe infections associated with high morbidity and mortality. Therefore, it is essential to gain more insight into the anti-fungal host defense response. The advent of omics technology and development of advanced systems biology tools has permitted to approach this in an unbiased and quanti...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章,评审

    doi:10.3389/fmicb.2016.00154

    authors: Smeekens SP,van de Veerdonk FL,Netea MG

    更新日期:2016-02-16 00:00:00

  • The in vitro Effect of Fibers With Different Degrees of Polymerization on Human Gut Bacteria.

    abstract::Human gut bacteria contribute significantly to human health and several studies have evaluated the effects of dietary fibers on human gut bacterial ecology. However, the relationship between different degrees of fiber polymerization and human gut bacteria is unknown. Here, we analyzed three fiber substrates with diffe...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2020.00819

    authors: Chen M,Fan B,Liu S,Imam KMSU,Xie Y,Wen B,Xin F

    更新日期:2020-05-15 00:00:00

  • Proteomic Analysis Reveals a Biofilm-Like Behavior of Planktonic Aggregates of Staphylococcus epidermidis Grown Under Environmental Pressure/Stress.

    abstract::Prosthetic joint replacement failure has a huge impact on quality of life and hospitalization costs. A leading cause of prosthetic joint infection is bacteria-forming biofilm on the surface of orthopedic devices. Staphylococcus epidermidis is an emergent, low-virulence pathogen implicated in chronic infections, barely...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2019.01909

    authors: Bottagisio M,Soggiu A,Piras C,Bidossi A,Greco V,Pieroni L,Bonizzi L,Roncada P,Lovati AB

    更新日期:2019-09-06 00:00:00

  • Effects of Wilting and Lactobacillus plantarum Addition on the Fermentation Quality and Microbial Community of Moringa oleifera Leaf Silage.

    abstract::The objective of this study was to evaluate the effects of wilting and Lactobacillus plantarum (LP) addition on the silage fermentation quality and microbial community of Moringa oleifera Lam. leaf silage. Unwilted (direct-cut) or wilted M. oleifera leaves were prepared either with or without LP (1.0 × 106 cfu/g) foll...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2018.01817

    authors: Wang Y,Wang C,Zhou W,Yang FY,Chen XY,Zhang Q

    更新日期:2018-08-06 00:00:00

  • Identification of Genes Essential for Antibiotic-Induced Up-Regulation of Plasmid-Transfer-Genes in Cephalosporin Resistant Escherichia coli.

    abstract::Bacterial conjugation is one of the most important mechanisms for spread of antibiotic resistance among bacteria. We have previously demonstrated that cefotaxime (CTX) exposure up-regulates expression of Type-IV conjugation transfer genes, and that this leads to increased transfer of a blaCTX-M-1 encoding IncI1 resist...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2019.02203

    authors: Liu G,Olsen JE,Thomsen LE

    更新日期:2019-09-24 00:00:00

  • Can Machines Learn Respiratory Virus Epidemiology?: A Comparative Study of Likelihood-Free Methods for the Estimation of Epidemiological Dynamics.

    abstract::To estimate and predict the transmission dynamics of respiratory viruses, the estimation of the basic reproduction number, R0, is essential. Recently, approximate Bayesian computation methods have been used as likelihood free methods to estimate epidemiological model parameters, particularly R0. In this paper, we expl...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2018.00343

    authors: Tessmer HL,Ito K,Omori R

    更新日期:2018-03-02 00:00:00

  • A Homeobox Transcription Factor UvHOX2 Regulates Chlamydospore Formation, Conidiogenesis, and Pathogenicity in Ustilaginoidea virens.

    abstract::Rice false smut fungus (teleomorph: Villosiclava virens; anamorph: Ustilaginoidea virens) can generate chlamydospores and survive winter under field conditions. The chlamydospore is considered as an important infection source of the disease. However, little is known about the regulatory mechanism of the chlamydospore ...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2019.01071

    authors: Yu J,Yu M,Song T,Cao H,Pan X,Yong M,Qi Z,Du Y,Zhang R,Yin X,Liu Y

    更新日期:2019-06-20 00:00:00

  • Co-existence of Methanogenesis and Sulfate Reduction with Common Substrates in Sulfate-Rich Estuarine Sediments.

    abstract::The competition between sulfate reducing bacteria and methanogens over common substrates has been proposed as a critical control for methane production. In this study, we examined the co-existence of methanogenesis and sulfate reduction with shared substrates over a large range of sulfate concentrations and rates of s...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2017.00766

    authors: Sela-Adler M,Ronen Z,Herut B,Antler G,Vigderovich H,Eckert W,Sivan O

    更新日期:2017-05-05 00:00:00

  • Deconjugated Bile Salts Produced by Extracellular Bile-Salt Hydrolase-Like Activities from the Probiotic Lactobacillus johnsonii La1 Inhibit Giardia duodenalis In vitro Growth.

    abstract::Giardiasis, currently considered a neglected disease, is caused by the intestinal protozoan parasite Giardia duodenalis and is widely spread in human as well as domestic and wild animals. The lack of appropriate medications and the spread of resistant parasite strains urgently call for the development of novel therape...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2016.01453

    authors: Travers MA,Sow C,Zirah S,Deregnaucourt C,Chaouch S,Queiroz RM,Charneau S,Allain T,Florent I,Grellier P

    更新日期:2016-09-27 00:00:00

  • Different metabolic features of Bacteroides fragilis growing in the presence of glucose and exopolysaccharides of bifidobacteria.

    abstract::Bacteroides is among the most abundant microorganism inhabiting the human intestine. They are saccharolytic bacteria able to use dietary or host-derived glycans as energy sources. Some Bacteroides fragilis strains contribute to the maturation of the immune system but it is also an opportunistic pathogen. The intestine...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2015.00825

    authors: Rios-Covian D,Sánchez B,Salazar N,Martínez N,Redruello B,Gueimonde M,de Los Reyes-Gavilán CG

    更新日期:2015-08-18 00:00:00

  • Functional Characterization of Cryptococcal Genes: Then and Now.

    abstract::Site-directed mutagenesis enables researchers to switch a gene of interest off for functional characterization of the gene. In the pathogenic yeasts, Cryptococcus neoformans and sister species C. deneoformans, this is almost exclusively achieved by introducing DNA into cells through either biolistic transformation or ...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章,评审

    doi:10.3389/fmicb.2018.02263

    authors: du Plooy LM,Sebolai OM,Pohl CH,Albertyn J

    更新日期:2018-09-20 00:00:00

  • Dynamics Relationship of Phyllosphere and Rhizosphere Bacterial Communities During the Development of Bothriochloa ischaemum in Copper Tailings.

    abstract::Copper mining and the byproducts associated with the industry have led to serious pollution in the Loess Plateau of China. There is a potential in improving the ecological restoration efficiency of such degraded land through combining microbial and plant remediation approaches. However, the community structure and fun...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2020.00869

    authors: Jia T,Yao Y,Wang R,Wu T,Chai B

    更新日期:2020-05-28 00:00:00

  • Functional Signatures of the Epiphytic Prokaryotic Microbiome of Agaves and Cacti.

    abstract::Microbial symbionts account for survival, development, fitness and evolution of eukaryotic hosts. These microorganisms together with their host form a biological unit known as holobiont. Recent studies have revealed that the holobiont of agaves and cacti comprises a diverse and structured microbiome, which might be im...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2019.03044

    authors: Flores-Núñez VM,Fonseca-García C,Desgarennes D,Eloe-Fadrosh E,Woyke T,Partida-Martínez LP

    更新日期:2020-01-17 00:00:00

  • Gastrointestinal Tract As Entry Route for Hantavirus Infection.

    abstract::Background: Hantaviruses are zoonotic agents that cause hemorrhagic fevers and are thought to be transmitted to humans by exposure to aerosolized excreta of infected rodents. Puumala virus (PUUV) is the predominant endemic hantavirus in Europe. A large proportion of PUUV-infected patients suffer from gastrointestinal ...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2017.01721

    authors: Witkowski PT,Perley CC,Brocato RL,Hooper JW,Jürgensen C,Schulzke JD,Krüger DH,Bücker R

    更新日期:2017-09-08 00:00:00

  • Inhibition of Rumen Methanogens by a Novel Archaeal Lytic Enzyme Displayed on Tailored Bionanoparticles.

    abstract::Methane is a potent greenhouse gas, 25 times more efficient at trapping heat than carbon dioxide. Ruminant methane emissions contribute almost 30% to anthropogenic sources of global atmospheric methane levels and a reduction in methane emissions would significantly contribute to slowing global temperature rises. Here ...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2018.02378

    authors: Altermann E,Schofield LR,Ronimus RS,Beatty AK,Reilly K

    更新日期:2018-10-09 00:00:00

  • In Vitro/Vivo Activity of Potential MCR-1 Inhibitor in Combination With Colistin Againsts mcr-1-Positive Klebsiella pneumonia.

    abstract::Carbapenem resistance among strains of the nosocomial pathogen Klebsiella pneumoniae is increasing worldwide, causing serious clinical infections and higher mortality rates. Polymyxins are some of the few "last resort" options for treatment of carbapenem-resistant Enterobacteriaceae, including K. pneumoniae, however, ...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2018.01615

    authors: Zhou Y,Wang T,Guo Y,Liu S,Wang J,Shen Y,Tang S,Wang Y,Deng X

    更新日期:2018-07-17 00:00:00

  • DNA Repair and Photoprotection: Mechanisms of Overcoming Environmental Ultraviolet Radiation Exposure in Halophilic Archaea.

    abstract::Halophilic archaea push the limits of life at several extremes. In particular, they are noted for their biochemical strategies in dealing with osmotic stress, low water activity and cycles of desiccation in their hypersaline environments. Another feature common to their habitats is intense ultraviolet (UV) radiation, ...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章,评审

    doi:10.3389/fmicb.2017.01882

    authors: Jones DL,Baxter BK

    更新日期:2017-09-29 00:00:00

  • An Integrative Genomic Island Affects the Adaptations of the Piezophilic Hyperthermophilic Archaeon Pyrococcus yayanosii to High Temperature and High Hydrostatic Pressure.

    abstract::Deep-sea hydrothermal vent environments are characterized by high hydrostatic pressure and sharp temperature and chemical gradients. Horizontal gene transfer is thought to play an important role in the microbial adaptation to such an extreme environment. In this study, a 21.4-kb DNA fragment was identified as a genomi...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2016.01927

    authors: Li Z,Li X,Xiao X,Xu J

    更新日期:2016-11-29 00:00:00

  • Diversity-Stability Dynamics of the Amphibian Skin Microbiome and Susceptibility to a Lethal Viral Pathogen.

    abstract::Variation among animals in their host-associated microbial communities is increasingly recognized as a key determinant of important life history traits including growth, metabolism, and resistance to disease. Quantitative estimates of the factors shaping the stability of host microbiomes over time at the individual le...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2019.02883

    authors: Harrison XA,Price SJ,Hopkins K,Leung WTM,Sergeant C,Garner TWJ

    更新日期:2019-12-20 00:00:00

  • Potential drivers of microbial community structure and function in Arctic spring snow.

    abstract::The Arctic seasonal snowpack can extend at times over a third of the Earth's land surface. This chemically dynamic environment interacts constantly with different environmental compartments such as atmosphere, soil and meltwater, and thus, strongly influences the entire biosphere. However, the microbial community asso...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2014.00413

    authors: Maccario L,Vogel TM,Larose C

    更新日期:2014-08-07 00:00:00

  • Quantifying Limits on Replication, Death, and Quiescence of Mycobacterium tuberculosis in Mice.

    abstract::When an individual is exposed to Mycobacterium tuberculosis (Mtb) three outcomes are possible: bacterial clearance, active disease, or latent infection. It is generally believed that most individuals exposed to Mtb become latently infected and carry the mycobacteria for life. How Mtb is maintained during this latent i...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2016.00862

    authors: McDaniel MM,Krishna N,Handagama WG,Eda S,Ganusov VV

    更新日期:2016-06-14 00:00:00

  • Differential NtcA Responsiveness to 2-Oxoglutarate Underlies the Diversity of C/N Balance Regulation in Prochlorococcus.

    abstract::Previous studies showed differences in the regulatory response to C/N balance in Prochlorococcus with respect to other cyanobacteria, but no information was available about its causes, or the ecological advantages conferred to thrive in oligotrophic environments. We addressed the changes in key enzymes (glutamine synt...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2017.02641

    authors: Domínguez-Martín MA,López-Lozano A,Clavería-Gimeno R,Velázquez-Campoy A,Seidel G,Burkovski A,Díez J,García-Fernández JM

    更新日期:2018-01-09 00:00:00

  • From Petri Dish to Patient: Bioavailability Estimation and Mechanism of Action for Antimicrobial and Immunomodulatory Natural Products.

    abstract::The new era of multidrug resistance of pathogens against frontline antibiotics has compromised the immense therapeutic gains of the 'golden age,' stimulating a resurgence in antimicrobial research focused on antimicrobial and immunomodulatory components of botanical, fungal or microbial origin. While much valuable inf...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章,评审

    doi:10.3389/fmicb.2019.02470

    authors: Sadgrove NJ,Jones GL

    更新日期:2019-10-31 00:00:00

  • Resistance Breeding of Common Bean Shapes the Physiology of the Rhizosphere Microbiome.

    abstract::The taxonomically diverse rhizosphere microbiome contributes to plant nutrition, growth and health, including protection against soil-borne pathogens. We previously showed that breeding for Fusarium-resistance in common bean changed the rhizosphere microbiome composition and functioning. Here, we assessed the impact o...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2019.02252

    authors: Mendes LW,de Chaves MG,Fonseca MC,Mendes R,Raaijmakers JM,Tsai SM

    更新日期:2019-10-01 00:00:00

  • An Assessment of Different Genomic Approaches for Inferring Phylogeny of Listeria monocytogenes.

    abstract::Background/objectives: Whole genome sequencing (WGS) has proven to be a powerful subtyping tool for foodborne pathogenic bacteria like L. monocytogenes. The interests of genome-scale analysis for national surveillance, outbreak detection or source tracking has been largely documented. The genomic data however can be e...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2017.02351

    authors: Henri C,Leekitcharoenphon P,Carleton HA,Radomski N,Kaas RS,Mariet JF,Felten A,Aarestrup FM,Gerner Smidt P,Roussel S,Guillier L,Mistou MY,Hendriksen RS

    更新日期:2017-11-29 00:00:00

  • Analysis of the Cell Type-Dependence on the Arenavirus Z-Mediated Virus-Like Particle Production.

    abstract::Several arenaviruses are highly pathogenic to humans, causing hemorrhagic fever. Discovery of anti-arenavirus drug candidates is urgently needed, although the molecular basis of the host- and organ-specific pathogenicity remains to be fully elucidated. The arenavirus Z protein facilitates production of virus-like part...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2020.562814

    authors: Mpingabo PI,Urata S,Yasuda J

    更新日期:2020-09-30 00:00:00

  • Mathematical Modeling Approaches for Assessing the Joint Toxicity of Chemical Mixtures Based on Luminescent Bacteria: A Systematic Review.

    abstract::Developments in industrial applications inevitably accelerate the discharge of enormous substances into the environment, whereas multi-component mixtures commonly cause joint toxicity which is distinct from the simple sum of independent effect. Thus, ecotoxicological assessment, by luminescent bioassays has recently b...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章,评审

    doi:10.3389/fmicb.2020.01651

    authors: Wang D,Wang S,Bai L,Nasir MS,Li S,Yan W

    更新日期:2020-07-31 00:00:00

  • De novo Transcriptome Analysis of Rhizoctonia solani AG1 IA Strain Early Invasion in Zoysia japonica Root.

    abstract::Zoysia japonica brown spot was caused by necrotrophic fungus Rhizoctonia solani invasion, which led to severe financial loss in city lawn and golf ground maintenance. However, little was known about the molecular mechanism of R. solani pathogenicity in Z. japonica. In this study we examined early stage interaction bet...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2016.00708

    authors: Zhu C,Ai L,Wang L,Yin P,Liu C,Li S,Zeng H

    更新日期:2016-05-18 00:00:00