Abstract：
:Macrophage Migration Inhibitory Factor (MIF) is the first human cytokine reported and was thought to have a central role in the regulation of inflammatory responses. Homologs of this molecule have been reported in bacteria, invertebrates and plants. Apart from cytokine activity, it also has two catalytic activities viz., tautomerase and di-sulfide oxidoreductase, which appear to be involved in immunological functions. The CXXC catalytic site is responsible for di-sulfide oxidoreductase activity of MIF. We have recently reported thiol-disulfide oxidoreductase activity of Macrophage Migration Inhibitory Factor-2 of Wuchereria bancrofti (Wba-MIF-2), although it lacks the CXXC motif. We hypothesized that three conserved cysteine residues might be involved in the formation of di-sulfide oxidoreductase catalytic site. Homology modeling of Wba-MIF-2 showed that among the three cysteine residues, Cys58 and Cys95 residues came in close proximity (3.23Å) in the tertiary structure with pKa value 9, indicating that these residues might play a role in the di-sulfide oxidoreductase catalytic activity. We carried out site directed mutagenesis of these residues (Cys58Ser & Cys95Ser) and expressed mutant proteins in Escherichia coli. The mutant proteins did not show any oxidoreductase activity in the insulin reduction assay, thus indicating that these two cysteine residues are vital for the catalytic activity of Wba-MIF-2.
journal_name
Acta Tropjournal_title
Acta tropicaauthors
Chauhan N,Hoti SLdoi
10.1016/j.actatropica.2015.09.017subject
Has Abstractpub_date
2016-01-01 00:00:00pages
14-20eissn
0001-706Xissn
1873-6254pii
S0001-706X(15)30114-5journal_volume
153pub_type
杂志文章相关文献
ACTA TROPICA文献大全abstract：:Exposure of 2 day old virgin females of Glossina fuscipes fuscipes, G. pallidipes, G. brevipalpis, and G. morsitans centralis to carbon dioxide anaesthesia for 15 sec has the effect of suppressing their subsequent insemination frequency. This insemination-inhibitory effect of the gas is more pronounced in G. f. fuscip...
journal_title：Acta tropica
pub_type： 杂志文章
doi：
更新日期：1975-01-01 00:00:00
abstract：:A survey was undertaken between November 1994 and January 1995 to assess the knowledge and practice with respect to malaria of 1946 randomly selected households in endemic urban and rural areas of Hormozgan, Kerman and Sistan and Baluchistan Provinces, southeast Iran. Knowledge of inhabitants was relatively poor in th...
journal_title：Acta tropica
pub_type： 杂志文章
doi：10.1016/s0001-706x(96)00609-2
更新日期：1997-04-15 00:00:00
abstract：:We focused on the epidemiology of alveolar echinococcosis especially in Japan and discussed control measures to prevent an epidemic. No effective control measures against alveolar echinococcosis have been identified thus far because it is difficult to fully understand the ecology of the parasite and its hosts, i.e. th...
journal_title：Acta tropica
pub_type： 杂志文章
doi：10.1016/j.actatropica.2003.09.001
更新日期：2003-12-01 00:00:00
abstract：:In the present investigation, it has been demonstrated that cattle become resistant to ticks after several heavy infestations, particularly with B. microplus. During development of the infestations, antibodies against salivary glands of B. microplus were detected using 2 techniques: indirect immunofluorescence and imm...
journal_title：Acta tropica
pub_type： 杂志文章
doi：
更新日期：1976-01-01 00:00:00
abstract：:With the paradigm shift from the reduction of morbidity and mortality to the interruption of transmission, the focus of malaria control broadens from symptomatic infections in children ≤5 years of age to include asymptomatic infections in older children and adults. In addition, as control efforts intensify and the num...
journal_title：Acta tropica
pub_type： 杂志文章
doi：10.1016/j.actatropica.2011.11.009
更新日期：2012-03-01 00:00:00
abstract：:The 4-year (2006-2010) alliance to optimize schistosomiasis control and transmission surveillance in sub-Saharan Africa (CONTRAST), funded by the European Commission, stands out as a 'game-changer' by successfully harnessing and combining new as well as previously available capabilities for schistosomiasis control in ...
journal_title：Acta tropica
pub_type： 杂志文章,评审
doi：10.1016/j.actatropica.2013.08.016
更新日期：2013-11-01 00:00:00
abstract：:The morphological and anatomical characters applied to determine species identity in the Bulinus africanus group species are insufficient to unambiguously discriminate between arbitrary species of given populations. In order to solve this problem, four snail populations from Kenya have been investigated morphologicall...
journal_title：Acta tropica
pub_type： 杂志文章
doi：10.1016/s0001-706x(99)00086-8
更新日期：2000-02-25 00:00:00
abstract：:393 primary school children were screened for Schistosoma haematobium using four indirect techniques (a) history of haematuria, (b) visual appearance of urine and use of chemical reagent strips to detect presence of (c) blood and (d) protein in urine. Results showed that the use of chemical reagent strips for the dete...
journal_title：Acta tropica
pub_type： 杂志文章
doi：
更新日期：1985-09-01 00:00:00
abstract：:Swine influenza is responsible for one of the most prevalent disease affecting the swine industry worldwide. Epidemiological surveys rarely focus on remote areas, because traditional farming systems characterized by locally consumed production and low pig densities are considered as having little influence on the emer...
journal_title：Acta tropica
pub_type： 杂志文章
doi：10.1016/j.actatropica.2011.11.012
更新日期：2012-04-01 00:00:00
abstract：:Malaria is second only to tuberculosis as the leading cause of morbidity and mortality as a consequence of a single infectious agent. Much of the pathology of malaria arises from the inappropriate or excessive immune response mounted by the host in an attempt to eliminate the parasite. We here report the inflammatory ...
journal_title：Acta tropica
pub_type： 杂志文章
doi：10.1016/j.actatropica.2011.04.020
更新日期：2011-10-01 00:00:00
abstract：:Chagas disease is a tropical parasitic disease endemic to Latin America which affects more than 10 million people, and kills more than 15,000 people each year. This condition, caused by the protozoan parasite Trypanosoma cruzi, is principally transmitted by kissing bugs (Triatominae). In Bolivia, recent reports by the...
journal_title：Acta tropica
pub_type： 杂志文章
doi：10.1016/j.actatropica.2010.10.005
更新日期：2011-02-01 00:00:00
abstract：:Toxoplasmosis is a common and serious parasitic disease with high prevalence and global distribution in human and other warm-blooded vertebrates. Though the infection of Toxoplasma gondii is usually asymptomatic in healthy people, it can lead to severe pathological effects to the fetus of infected women and immunocomp...
journal_title：Acta tropica
pub_type： 杂志文章,meta分析,评审
doi：10.1016/j.actatropica.2016.03.003
更新日期：2016-06-01 00:00:00
abstract：:The major international guidelines for health research have come a long way in setting standards and providing direction on acceptable practices for the conduct of research. However, the responsibilities of the key players have generally been addressed in a rather uncoordinated manner, and there still remain gaps in a...
journal_title：Acta tropica
pub_type： 杂志文章,评审
doi：10.1016/j.actatropica.2009.08.004
更新日期：2009-11-01 00:00:00
abstract：:Trematodes belonging to the superfamily Diplostomoidea have complex life cycles involving birds, mammals and reptiles as definitive hosts, and gastropods and different groups of invertebrates and vertebrates as intermediate hosts. Molecular studies of these parasites are numerous, but data from larval stages in mollus...
journal_title：Acta tropica
pub_type： 杂志文章
doi：10.1016/j.actatropica.2019.105082
更新日期：2019-11-01 00:00:00
abstract：:The Kato-Katz technique is the method routinely used for diagnosing human schistosomiasis mansoni by estimating faecal egg burdens. To improve the sensitivity of faecal diagnosis, we established and validated a novel separation technique based upon the greater density of viable schistosome eggs relative to faecal mate...
journal_title：Acta tropica
pub_type： 杂志文章
doi：10.1016/s0001-706x(02)00089-x
更新日期：2002-08-01 00:00:00
abstract：:The present study was undertaken to characterize more thoroughly aetiological agent of cystic echinococcosis that still represents a health problem in the Slovak Republic. Sequencing of the mitochondrial ND1 gene, Random Amplified Polymorphic DNA (RAPD) analysis and isoenzyme analyses were used to characterize genetic...
journal_title：Acta tropica
pub_type： 杂志文章
doi：10.1016/s0001-706x(02)00229-2
更新日期：2003-02-01 00:00:00
abstract：:Trypanosoma rangeli, a non-pathogenic hemoflagelate that in Central and South America infects humans, shares with Trypanosoma cruzi reservoirs and triatomine vectors, as well as geographical distribution. Recently, we have described in T. rangeli a truncated gene copy belonging to the group II of the trans-sialidase s...
journal_title：Acta tropica
pub_type： 杂志文章
doi：10.1016/j.actatropica.2009.05.003
更新日期：2009-09-01 00:00:00
abstract：:Using immunoassay and immunoblotting approaches, antibodies to Sj26, a glutathione S-transferase molecule (Mr = 26,000) of Schistosoma japonicum worms that is a vaccine candidate, have been sought in three large human serum banks. For these studies, a near-native recombinant Sj26 molecule produced in Escherichia coli ...
journal_title：Acta tropica
pub_type： 杂志文章
doi：10.1016/0001-706x(89)90023-5
更新日期：1989-07-01 00:00:00
abstract：:Malaria kills more than a million people a year, causes malady in about three hundred million people and poses risk to approximately 40% of the world's population living in malarious countries. This disease is re-emerging mainly due to the development of drug-resistant parasites and insecticide-resistant mosquitoes. T...
journal_title：Acta tropica
pub_type： 杂志文章,评审
doi：10.1016/j.actatropica.2009.10.024
更新日期：2010-06-01 00:00:00
abstract：:A study of host preference in tsetse flies using a modified heteroduplex PCR-based method is described. Domestic and wild animal blood samples were collected to extract the corresponding reference DNAs. In Campo (south Cameroon), tsetse flies (mainly Glossina palpalis palpalis) were trapped and 41 bloodmeals were coll...
journal_title：Acta tropica
pub_type： 杂志文章
doi：10.1016/j.actatropica.2004.03.006
更新日期：2004-07-01 00:00:00
abstract：:Neurocysticercosis (NC) is a serious public health problem mainly in developing countries. NC caused by the cysticercus stage from cestode Taenia solium is considered by the WHO and ITFDE as a potentially eradicable disease. Definitive diagnosis of NC is challenging because of the unspecific clinical manifestations su...
journal_title：Acta tropica
pub_type： 杂志文章
doi：10.1016/j.actatropica.2015.11.001
更新日期：2016-02-01 00:00:00
abstract：:Studies on in vitro skin permeation and in vivo anti-leishmanial activity in mice experimentally infected with Leishmania (Leishmania) major pointed out to the potential of a new paromomycin (PA) formulation (hydrophilic gel) for treatment of cutaneous leishmaniasis (CL). In this study, the activity of this formulatio...
journal_title：Acta tropica
pub_type： 杂志文章
doi：10.1016/j.actatropica.2004.10.007
更新日期：2005-02-01 00:00:00
abstract：:It has been reported a higher seroprevalence of HBV and HCV in leprosy patients than in the general population, but the reasons for these findings are not yet clear. On the other hand, there is evidence that these viruses may influence the onset of leprosy reactional episodes, an important cause of neurological sequel...
journal_title：Acta tropica
pub_type： 杂志文章
doi：10.1016/j.actatropica.2017.04.024
更新日期：2017-08-01 00:00:00
abstract：:In dogs, information on treatments against S. stercoralis infection is rare and anecdotal. The aim of the present work was to evaluate the treatment outcome of S. stercoralis natural infection in sheltered dogs. Furthermore, based on the potential risk of infection, people working in the infected shelter were also tes...
journal_title：Acta tropica
pub_type： 杂志文章
doi：10.1016/j.actatropica.2018.11.014
更新日期：2019-02-01 00:00:00
abstract：:Entomopathogenic fungi, especially Metarhizium anisopliae, have potential for integrated control of peridomestic triatomine bugs. However, the high susceptibility of these vectors to fungal infection at elevated ambient humidities decreases in the comparatively dry conditions that often prevail in their microhabitats....
journal_title：Acta tropica
pub_type： 杂志文章
doi：10.1016/j.actatropica.2011.11.014
更新日期：2012-04-01 00:00:00
abstract：:The several steps in the pathway for the biosynthesis of fibrillar collagen are reviewed to illustrate potential sites for the chemotherapeutic control of fibrosis. Particular emphasis is placed upon the properties and inhibition of lysyl oxidase, the enzyme which initiates the covalent crosslinking of extracellular c...
journal_title：Acta tropica
pub_type： 杂志文章,评审
doi：10.1016/s0001-706x(00)00128-5
更新日期：2000-10-23 00:00:00
abstract：:Blastospores or conidia (formulated or not) of entomopathogenic fungi were assessed against Aedes aegypti larvae. Larvae (L2) were exposed to 105, 106, 107, and 108 propagules mL-1 water suspension. Mineral oil at 0.1%, 0.5%, or 1.0% (v/v) was employed to observe the effect on larval survival. The 0.1% mineral oil did...
journal_title：Acta tropica
pub_type： 杂志文章
doi：10.1016/j.actatropica.2020.105732
更新日期：2021-01-01 00:00:00
abstract：:In addition to genetic history, environmental conditions during larval stages are critical to the development, success and phenotypic fate of the Aedes aegypti mosquito. In particular, previous studies have shown a strong genotype-by-environment component to adult mosquito body size in response to optimal vs stressed ...
journal_title：Acta tropica
pub_type： 杂志文章
doi：10.1016/j.actatropica.2017.06.018
更新日期：2017-10-01 00:00:00
abstract：:The efficacy of a 5-day treatment with intramuscular artemether (3.2-mg/kg loading dose followed by 1.6 mg/kg daily) was compared to that of the standard 7-day treatment with quinine (20-mg/kg loading dose followed by 10 mg/kg every 8 h) in a randomised clinical trial including 103 children aged 12-60 months with cere...
journal_title：Acta tropica
pub_type： 临床试验,杂志文章,随机对照试验
doi：10.1016/s0001-706x(99)00031-5
更新日期：1999-10-15 00:00:00
abstract：:A 30-kDa surface collagen binding protein peroxiredoxin of Entamoeba histolytica (EhCBP30) was evaluated either alone or fused to the chaperone (CHP) or ATPase (ATP) domains of heat shock protein 70 of Trypanosoma cruzi (TcHSP70) as a vaccine candidate in a hamster model of experimental amoebic liver abscess (ALA) dev...
journal_title：Acta tropica
pub_type： 杂志文章
doi：10.1016/j.actatropica.2011.12.007
更新日期：2012-05-01 00:00:00
