Abstract：
:Endothelial injury is regarded as the initial pathological process in diabetic vascular diseases, but effective therapy has not yet been identified. Although β-hydroxybutyrate plays various protective roles in the cardiovascular system, its ability to antagonize diabetic endothelial injury is unclear. β-hydroxybutyrate reportedly causes histone H3K9 β-hydroxybutyrylation (H3K9bhb), which activates gene expression; however, there has been no report regarding the role of H3K9bhb in up-regulation of vascular endothelial growth factor (VEGF), a crucial factor in endothelial integrity and function. Here, male Sprague-Dawley rats were intraperitoneally injected with streptozotocin to induce diabetes, and then treated with different concentrations of β-hydroxybutyrate. After 10 weeks, body weight, blood glucose, morphological changes and serum nitric oxide concentration were examined. Moreover, the mRNA expression level, protein content and distribution of VEGF in the aorta were investigated, as were total protein β-hydroxybutyrylation and H3K9bhb contents. The results showed injury of aortic endothelium, along with reductions of the concentration of nitric oxide and generation of VEGF in diabetic rats. However, β-hydroxybutyrate treatment attenuated diabetic injury of the endothelium and up-regulated the generation of VEGF. Furthermore, β-hydroxybutyrate treatment caused marked total protein β-hydroxybutyrylation and significant elevation of H3K9bhb content in the aorta of diabetic rats. The ability of β-hydroxybutyrate to protect against diabetic injury of the aortic endothelium was greatest for its intermediate concentration. In conclusion, moderately elevated β-hydroxybutyrate could antagonize aortic endothelial injury, potentially by causing H3K9bhb to promote generation of VEGF in diabetic rats.
journal_name
Tissue Celljournal_title
Tissue & cellauthors
Wu X,Miao D,Liu Z,Liu K,Zhang B,Li J,Li Y,Qi Jdoi
10.1016/j.tice.2020.101345subject
Has Abstractpub_date
2020-06-01 00:00:00pages
101345eissn
0040-8166issn
1532-3072pii
S0040-8166(19)30406-9journal_volume
64pub_type
杂志文章相关文献
TISSUE & CELL文献大全abstract：:Examination of livers from transgenic mice over expressing human growth hormone (hGH) revealed numerous alterations including a striking incidence of mitotic figures. The reason for increased proliferation is unclear, but could be related to effects of hGH, which is also acting as a lactogen in rodents. In order to id...
journal_title：Tissue & cell
pub_type： 杂志文章
doi：10.1016/0040-8166(94)90029-9
更新日期：1994-06-01 00:00:00
abstract：:Testicular peritubular tissue, also known as the tunica propria, surrounds the seminiferous tubules and is responsible for contractile, paracrine and transport functions. The aim of the present report is to describe the pathomorphology of peritubular tissue in association with tubular hyalinization in human testis. Tw...
journal_title：Tissue & cell
pub_type： 临床试验,杂志文章
doi：10.1054/tice.1999.0005
更新日期：1999-02-01 00:00:00
abstract：:Time lapse cinematography was used to study and compare the proliferation and migration activity of pulmonary endothelial cells and fibroblasts, two cell types with very different structural and functional properties. Endothelial cells were found to have a more rapid growth rate than fibroblasts. Contributing to the s...
journal_title：Tissue & cell
pub_type： 杂志文章
doi：10.1016/s0040-8166(81)80002-x
更新日期：1981-01-01 00:00:00
abstract：:The number of epithelial cells comprising larval midgut of the tobacco hornworm moth, Manduca sexta increases 200-fold in development from the first to the fifth instar. We have examined larvae periodically before and during molting to follow epithelial cell proliferation and differentiation. The midgut epithelium in ...
journal_title：Tissue & cell
pub_type： 杂志文章
doi：10.1016/0040-8166(91)90058-2
更新日期：1991-01-01 00:00:00
abstract：:The surface topologies of mouse adrenal cortex tumor cells of primary or clonal origin grown as monolayer cell cultures were observed by scanning electron microscopy following their exposure to substances that effect steroid release and/or cell rounding. ACTH induced cell rounding with a concomitant profuse developmen...
journal_title：Tissue & cell
pub_type： 杂志文章
doi：10.1016/0040-8166(77)90034-9
更新日期：1977-01-01 00:00:00
abstract：:To obtain information about the chemical composition of pore structures in antennal sensilla, the antennae were exposed to lipid solvents, or they were prepared to show negative-contrast images in electron micrographs. A heavy-metal tracer, lanthanum nitrate, was also used to indicate the permeability of the receptors...
journal_title：Tissue & cell
pub_type： 杂志文章
doi：10.1016/s0040-8166(71)80012-5
更新日期：1971-01-01 00:00:00
abstract：:The distribution of F-actin and intermediate filaments in the W/WV mouse was investigated by light and transmission electron microscopy, and fluorescence methods. No spermatogenic cells were detected in the seminiferous epithelium of the W/WV mouse. Its seminiferous tubule was one-half the diameter of that in the norm...
journal_title：Tissue & cell
pub_type： 杂志文章
doi：10.1016/0040-8166(92)90024-2
更新日期：1992-01-01 00:00:00
abstract：:Acute spinal cord injuries (ASCI) are common neural disorders in traumatology medicine. MicroRNA-210 (miR-210) plays a crucial role in cell survival, endothelial cell migration and cell regeneration. This paper is aim to validate the pathophysiological function of miR-210 on ASCI. We built a rat model of ASCI and util...
journal_title：Tissue & cell
pub_type： 杂志文章
doi：10.1016/j.tice.2019.02.004
更新日期：2019-04-01 00:00:00
abstract：:Rhodnius prolixus is an important vector of Trypanosoma cruzi, the etiological agent of Chagas disease. Insect adults have a pair of Metasternal Glands (MGs) and the secretion emitted by these glands acts as sex pheromone. Recent studies have focused on the chemical composition of this pheromone, electrophysiological ...
journal_title：Tissue & cell
pub_type： 杂志文章
doi：10.1016/j.tice.2020.101416
更新日期：2020-12-01 00:00:00
abstract：:Using microsurgical manipulations, hormone applications, and transmission electron microscopy we have investigated the regulation of differentiation of the follicular epithelium and formation of the vitelline envelope (VE) in primary follicles in the ovary of the mosquito, Aedes aegypti. During the first 3 days after ...
journal_title：Tissue & cell
pub_type： 杂志文章
doi：10.1016/0040-8166(91)90015-l
更新日期：1991-01-01 00:00:00
abstract：:Zebra Finches are the epitome of desert-adapted avian species; i.e. they are able to survive without drinking water for over a year. Whereas transepidermal water loss (TEWL) in naked Zebra Finch nestlings is lower than in adults, and desert adaptation is accompanied by intercellular deposition of epidermal multigranul...
journal_title：Tissue & cell
pub_type： 杂志文章
doi：10.1016/0040-8166(89)90023-2
更新日期：1989-01-01 00:00:00
abstract：:Research spanning almost 50 years has highlighted unique characteristics and irreplaceable list of diverse functions performed by peroxisomes in various model systems. Peroxisomes are single membrane bound highly dynamic organelles ubiquitous to most eukaryotic cells. Proliferation by division of pre-existing organell...
journal_title：Tissue & cell
pub_type： 杂志文章,评审
doi：10.1016/j.tice.2017.03.002
更新日期：2017-04-01 00:00:00
abstract：:Induction of NF-κB-mediated gene expression has been identified in the pathogenesis of alcoholic liver disease (ALD). Diethylcarbamazine (DEC) is a piperazine derivative drug with anti-inflammatory properties. The present study was designed to evaluate the effect of DEC on NF-κB pathways in mice undergoing alcoholism ...
journal_title：Tissue & cell
pub_type： 杂志文章
doi：10.1016/j.tice.2014.06.008
更新日期：2014-10-01 00:00:00
abstract：:Using a monoclonal antibody (GT 335) we previously demonstrated that glutamylation is a predominant posttranslational modification of alpha and beta tubulin isoforms in the axoneme of mouse spermatozoa (Fouquet et al., Cell Motil. Cytoskel. 27, 49, 1994). However, we noted that the staining intensity and/or distributi...
journal_title：Tissue & cell
pub_type： 杂志文章
doi：10.1016/s0040-8166(95)80053-0
更新日期：1995-06-01 00:00:00
abstract：:The existence and distribution of mammosomatotrophs (MS cells) containing growth hormone (GH) and prolactin (PRL) in bovine adenohypophysis were detailed by a combined method of mirror sections and immunohistochemical staining. MS cells always occurred in bovine adenohypophysis but their number was low. In the midsagi...
journal_title：Tissue & cell
pub_type： 杂志文章
doi：10.1054/tice.1999.0051
更新日期：1999-10-01 00:00:00
abstract：:Myosin as well as actin filaments could be seen in negatively stained preparations of fresh chicken gizzard homogenized in buffered KCI (I=0.12, pH 6.85), in a 1 : 1 ratio. Myosin filaments were also present when homogenates were diluted (1 : 9) with solutions containing additional Mg(++) and ATP provided naturally oc...
journal_title：Tissue & cell
pub_type： 杂志文章
doi：10.1016/s0040-8166(69)80007-8
更新日期：1969-01-01 00:00:00
abstract：:The sensory organs on the tarsi of the antenniform first legs of the whip spider Admetus pumilio C. L. Koch (Amblypygi, Arachnida) were examined with the scanning and transmission electron microscope. At least four different types of hair sensilla were found: (1) thick-walled bristles, which have the characteristics o...
journal_title：Tissue & cell
pub_type： 杂志文章
doi：10.1016/0040-8166(75)90009-9
更新日期：1975-01-01 00:00:00
abstract：:The expression of alpha(1a)-adrenoreceptors (alpha(1a)-ARs) within the muscle spindles of rabbit masseter muscle was investigated. The alpha(1a)-ARs were detected by immunohistochemical fluorescent method and examined along the entire length of 109 cross serially sectioned spindles. The sympathetic fibers were visuali...
journal_title：Tissue & cell
pub_type： 杂志文章
doi：10.1016/j.tice.2005.12.003
更新日期：2006-04-01 00:00:00
abstract：:Collodial gold coated with the glycoprotein, bovine submaxillary mucin (BSM-gold), was used to localize chemoreceptors known to be involved in triggering the discharge of cnidae in sea anemones. BSM-gold binds exclusively at the apical surface of the supporting cell, the cell adjacent to the cnidocyte (Watson and Hess...
journal_title：Tissue & cell
pub_type： 杂志文章
doi：10.1016/0040-8166(87)90016-4
更新日期：1987-01-01 00:00:00
abstract：:In the adult male rat, hemicastration (HC) induces a remarkable enhancement of testosterone secretion from the Leydig cells of the remaining testis. We have observed previously that the denervation of inferior spermatic nerves inhibits this enhancement. The present experiments were designed to assess morphometrically ...
journal_title：Tissue & cell
pub_type： 杂志文章
doi：10.1016/s0040-8166(98)80027-x
更新日期：1998-10-01 00:00:00
abstract：:This study evaluated ulceroprotective and antioxidant effect of 1,25 dihydroxyvitamin D3 against gastric damage in rats. Rats were treated intraperitoneally with either 1,25 dihydroxyvitamin D3 (0.25 μg/kg) or saline for 14 days. On day-15, the non-selective cyclooxygenase inhibitor indomethacin (10 mg/kg; subcutaneou...
journal_title：Tissue & cell
pub_type： 杂志文章
doi：10.1016/j.tice.2018.03.003
更新日期：2018-04-01 00:00:00
abstract：:The second half of the 20th century has witnessed the birth and growth of biological ultrastructure research--a branch of cell biology in which electron microscopy plays an important role. After a humble start in around 1950, when only a limited arsenal of instrumentation was available, a wealth of auxiliary methodolo...
journal_title：Tissue & cell
pub_type： 历史文章,杂志文章,评审
doi：10.1016/j.tice.2003.11.001
更新日期：2004-04-01 00:00:00
abstract：:Responses of cells in the tick salivary gland to parasitism by Theileria parva were studied by electron microscopy. The gland is composed of three distinct types of acini (I, II, III) which together include ten or more different cell types. Of some 30 infected cells observed in the present study, all were E-cells of a...
journal_title：Tissue & cell
pub_type： 杂志文章
doi：10.1016/0040-8166(82)90035-0
更新日期：1982-01-01 00:00:00
abstract：:Three different metal salts, silver nitrate, uranyl acetate and lead citrate, are mixed with a collagen gel to produce 3 metal/collagen sponges. These sponges were implanted subcutaneously in the rat and samples harvested after 5 days of implantation. TEM observation shows that sponges are degraded and digested by mac...
journal_title：Tissue & cell
pub_type： 杂志文章
doi：10.1016/0040-8166(90)90092-n
更新日期：1990-01-01 00:00:00
abstract：:The androgenic glands (AG) of male decapod crustaceans produce insulin-like androgenic gland (IAG) hormone that controls male sex differentiation, growth and behavior. Functions of the AG are inhibited by gonad-inhibiting hormone originating from X-organ-sinus gland complex in the eyestalk. The AG, and its interaction...
journal_title：Tissue & cell
pub_type： 杂志文章
doi：10.1016/j.tice.2010.07.003
更新日期：2010-10-01 00:00:00
abstract：OBJECTIVE:The current study proposes an automated machine learning approach for the quantification of cells in cell death pathways according to DNA fragmentation. METHODS:A total of 17 images of kidney histological slide samples from male Wistar rats were used. The slides were photographed using an Axio Zeiss Vert.A1 ...
journal_title：Tissue & cell
pub_type： 杂志文章
doi：10.1016/j.tice.2016.12.006
更新日期：2017-02-01 00:00:00
abstract：:The distribution of calcified microdeposits in non-atherosclerotic intima of the human aorta was studied by electron microscopy. Aortic specimens were obtained during aortic reconstruction and were embedded in Lowicryl resin. Non-stained ultrathin sections were analysed using an electron microscope equipped with an en...
journal_title：Tissue & cell
pub_type： 杂志文章
doi：10.1016/s0040-8166(98)80052-9
更新日期：1998-06-01 00:00:00
abstract：:Pyridoxal-5'-phosphate, the active form of vitamin B6, is associated with activities of several enzymes and the treatment of various neurological disorders. Here, we investigated the effects of pyridoxine on the immunoreactivity and protein levels of γ-aminobutyric acid (GABA)-synthesizing and degradation enzymes such...
journal_title：Tissue & cell
pub_type： 杂志文章
doi：10.1016/j.tice.2019.09.005
更新日期：2019-12-01 00:00:00
abstract：:In tendon lesions, inflammation indicates the beginning of tissue repair and influences cell proliferation and the remodeling of the extracellular matrix (ECM). Low level laser (LLL) therapy has been an important method to induce tissue repair, and several studies have sought to better understand the therapeutic possi...
journal_title：Tissue & cell
pub_type： 杂志文章
doi：10.1016/j.tice.2017.05.006
更新日期：2017-08-01 00:00:00
abstract：:Reptilian epidermis contains two types of keratin, soft (alpha) and hard (beta). The biosynthesis and molecular weight of beta-keratin during differentiation of lizard epidermis have been studied by autoradiography, immunocytochemistry and immunoblotting. Tritiated proline is mainly incorporated into differentiating a...
journal_title：Tissue & cell
pub_type： 杂志文章
doi：10.1016/j.tice.2004.06.001
更新日期：2004-10-01 00:00:00
